Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,315 C→G 5.9% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3150CG 5.9% 780.6 / 30.8 223intergenic (+28/+75)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (103/106);  new base G (5/8);  total (109/114)
Fisher's exact test for biased strand distribution p-value = 5.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 228 total aligned reads displayed.

CGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCCATCGTCATTAACTTTCACCTGGTTAGAATCGGC  >  NC_000913/2286050‑2286587
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                 
cGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttt                                                                                                                                                                                                                                                                          >  2:679158/1‑275 (MQ=255)
      gaaaaggtgaaagaTGAAAAACCACATTTAAGTTTTTTATTGCAAGTGCTGACAGACGGGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtat                                                                                                                                                                                                                                                                    <  4:129272/269‑1 (MQ=255)
                             acaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAAGCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATACCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACa                                                                                                                                                                                                                                             <  2:613546/275‑1 (MQ=255)
                                          ttttttcAAGTTCTTCCAGAAGAGAATCGTTTTTTCTCGAACTGTTTCATTATAAATCAGTTAGCGAAAGATCTTCCTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGCGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTAGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACAt                                                                                                                                                                                                                               <  4:679153/269‑1 (MQ=255)
                                              tGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCCATTACTTCGATAAACAGACCGTG‑ACACATca                                                                                                                                                                                                                             >  2:338089/1‑273 (MQ=255)
                                                              cGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTACCGTGCCGCCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATATGCACTTAATTCGATCAACA‑ACCCTGTACCCATCACATCCTGTTTAtttttt                                                                                                                                                                                                            >  4:190125/1‑272 (MQ=255)
                                                                                      attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCa                                                                                                                                                                                                                                                             >  4:264354/1‑202 (MQ=255)
                                                                                           ttaTAAATCAGTTAGCGAAAGATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCAACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                                                                                                                                                              <  2:599297/275‑1 (MQ=255)
                                                                                              taAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGATCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACAGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCGGACCCAACCCGCCTGAg                                                                                                                                                                           >  4:430980/1‑275 (MQ=255)
                                                                                                                    tACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ac                                                                                                                                                                                                                                   >  2:499718/1‑197 (MQ=255)
                                                                                                                         gCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggtt                                                                                                                                                                                                                                                                               >  6:550090/1‑149 (MQ=255)
                                                                                                                           aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttgg                                                                                                                                                                                                                                                                             <  5:226272/149‑1 (MQ=255)
                                                                                                                            aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCTTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggt                                                                                                                                                                                                                                                                            <  5:1149150/149‑1 (MQ=255)
                                                                                                                            aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                                                                                                                                                            <  8:66970/149‑1 (MQ=255)
                                                                                                                            aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                                                                                                                                                            <  7:1488752/149‑1 (MQ=255)
                                                                                                                             tCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                                                                                                                                                           <  5:1239993/149‑1 (MQ=255)
                                                                                                                              cGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttt                                                                                                                                                                                                                                                                          <  5:493986/149‑1 (MQ=255)
                                                                                                                                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                                                                                                                                                        <  8:1350831/149‑1 (MQ=255)
                                                                                                                                gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                                                                                                                                                        <  7:895122/149‑1 (MQ=255)
                                                                                                                                gtatgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                                                                                                                                                        <  7:527147/146‑1 (MQ=255)
                                                                                                                                 tgtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                                                                                                                                                       <  5:550090/149‑1 (MQ=255)
                                                                                                                                  gtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttttt                                                                                                                                                                                                                                                                      >  5:1104495/1‑149 (MQ=255)
                                                                                                                                        aaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCt                                                                                                                                                                                                                                                                >  7:1021731/1‑149 (MQ=255)
                                                                                                                                         aCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctgac                                                                                                                                <  2:195981/275‑1 (MQ=255)
                                                                                                                                           ggTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttt                                                                                                                                                                                                                                                                          >  8:1260863/1‑136 (MQ=255)
                                                                                                                                           ggTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggt                                                                                                                                                                                                                                                                                <  1:3741/130‑1 (MQ=255)
                                                                                                                                           ggTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCa                                                                                                                                                                                                                                                             >  6:1392790/1‑149 (MQ=255)
                                                                                                                                            gtagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                                                                                                                                                       <  6:815260/138‑1 (MQ=255)
                                                                                                                                             tagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAtttt                                                                                                                                                                                                              <  1:232545/193‑1 (MQ=255)
                                                                                                                                             tagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGCCGATTCTACCCCTGCGCCATTGCCATAGctgactga                                                                                                                             >  2:426224/1‑274 (MQ=255)
                                                                                                                                                  ttAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaatt                                                                                                                                                                                                                                                      >  6:489690/1‑149 (MQ=255)
                                                                                                                                                      cagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                                                                                                                                                                  <  5:1519296/149‑1 (MQ=255)
                                                                                                                                                       atccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAACTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCATACCACACCCGCCTGAGTATTCCACGGCGATTCTCACCCTGCGCCATTGCCATAGCTGACTGACACATGAcctc                                                                                                                  >  2:144200/3‑273 (MQ=255)
                                                                                                                                                         ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                                                                                                                                                               >  8:1064994/1‑149 (MQ=255)
                                                                                                                                                          cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                                                                                                                                                       <  7:1202786/124‑1 (MQ=255)
                                                                                                                                                          cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTGTATCTGCAATTAATTCGATAAAc                                                                                                                                                                                                                                              <  6:415370/149‑1 (MQ=255)
                                                                                                                                                          cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTNTATATCTGCAATTAATTCGATAANCAGANCCTG‑ACACATCAAAGCCTGTTTATTTTCTGTTATCAGCACGTCCAGACCacac                                                                                                                                                                                    >  4:703317/1‑206 (MQ=255)
                                                                                                                                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                                                                                                                                                   >  6:481711/1‑111 (MQ=255)
                                                                                                                                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                                                                                                                                                   <  5:481711/111‑1 (MQ=255)
                                                                                                                                                           aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                                                                                                                                                                                                              >  6:1652866/1‑148 (MQ=255)
                                                                                                                                                              aGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc                                                                                                                                                                                              <  2:703322/192‑1 (MQ=255)
                                                                                                                                                                 cGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                                                                                                                                                    >  6:777189/1‑136 (MQ=255)
                                                                                                                                                                     aCGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑aca                                                                                                                                                                                                                                  >  7:1085271/1‑149 (MQ=255)
                                                                                                                                                                      cGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑acac                                                                                                                                                                                                                                 >  8:1621526/1‑149 (MQ=255)
                                                                                                                                                                       gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑Acaca                                                                                                                                                                                                                                >  8:2912/1‑149 (MQ=255)
                                                                                                                                                                           cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGAt                                                                                              <  2:281940/275‑1 (MQ=255)
                                                                                                                                                                           cgcAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGAt                                                                                              <  2:190128/275‑1 (MQ=255)
                                                                                                                                                                                  tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGt                                                                                                                                                                                                                     <  6:256570/149‑1 (MQ=255)
                                                                                                                                                                                   ggTAGCGCACCATCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGtt                                                                                                                                                                                                                    <  8:710868/149‑1 (MQ=255)
                                                                                                                                                                                      aGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAt                                                                                                                                                                                                                 >  7:1562667/1‑149 (MQ=255)
                                                                                                                                                                                        cgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAttt                                                                                                                                                                                                               <  8:1562667/149‑1 (MQ=255)
                                                                                                                                                                                         gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAtttt                                                                                                                                                                                                              <  5:806840/149‑1 (MQ=255)
                                                                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑a                                                                                                                                                                                                                                    >  7:1662435/1‑125 (MQ=255)
                                                                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑a                                                                                                                                                                                                                                    <  8:1662435/125‑1 (MQ=255)
                                                                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAtt                                                                                                                                                                                                                <  5:1664822/145‑1 (MQ=255)
                                                                                                                                                                                           aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                                                            >  6:942831/1‑149 (MQ=255)
                                                                                                                                                                                             cGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTAtt                                                                                                                                                                                                                >  4:480630/1‑143 (MQ=255)
                                                                                                                                                                                              gTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                                                                                                                                                <  1:102882/174‑1 (MQ=255)
                                                                                                                                                                                              gTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                                                                                                                                                >  3:102882/1‑174 (MQ=255)
                                                                                                                                                                                               tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTc                                                                                                                                                                                                             >  7:1629015/1‑144 (MQ=255)
                                                                                                                                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                                                                                                                                                           <  6:1204537/81‑1 (MQ=38)
                                                                                                                                                                                                 aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTCCTTTTTTc                                                                                                                                                                                                      >  6:1254645/1‑149 (MQ=255)
                                                                                                                                                                                                    gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGa                                                                                                                                                                                                   >  5:778580/1‑149 (MQ=255)
                                                                                                                                                                                                     ggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGaa                                                                                                                                                                                                  <  5:1392790/149‑1 (MQ=255)
                                                                                                                                                                                                          cGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTcc                                                                                                                                                                                             <  5:1196327/149‑1 (MQ=255)
                                                                                                                                                                                                           gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCg                                                                                                                                                                                                                                        >  4:315339/1‑106 (MQ=255)
                                                                                                                                                                                                           gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCa                                                                                                                                                                                            >  6:1410724/1‑149 (MQ=255)
                                                                                                                                                                                                             gggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACg                                                                                                                                                                                                                                                                                  <  2:461645/62‑1 (MQ=255)
                                                                                                                                                                                                              ggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAc                                                                                                                                                                                         >  5:876442/1‑149 (MQ=255)
                                                                                                                                                                                                                tCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCt                                                                                                                                                                                                            >  7:241209/1‑128 (MQ=255)
                                                                                                                                                                                                                   gAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCacac                                                                                                                                                                                    <  5:282390/149‑1 (MQ=255)
                                                                                                                                                                                                                      gTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCaaacttg                                                                                                                                                                                 >  7:1338444/1‑146 (MQ=255)
                                                                                                                                                                                                                      gTTCAAATCCTCTCGTGCCGACCAAAAAACCCAAGAAAAATCCAGACCTTACAGTTGGTTTTGTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTTTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCAcat                                                   <  2:246404/275‑1 (MQ=255)
                                                                                                                                                                                                                       ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTc                                                                                                                                                                                              <  2:569702/135‑1 (MQ=255)
                                                                                                                                                                                                                       ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                                                                                                                                                >  7:20212/1‑149 (MQ=255)
                                                                                                                                                                                                                          aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                                                                                                                                                                                                   >  5:957415/1‑64 (MQ=255)
                                                                                                                                                                                                                          aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTg                                                                                                                                                                             >  6:1465705/1‑149 (MQ=255)
                                                                                                                                                                                                                           aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACGCATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                                                                                                                                                            >  5:677913/1‑149 (MQ=255)
                                                                                                                                                                                                                               ctctCGTGCCGACCAAAAATACCAAGAAAACACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAt                                                                                                                                                                        <  6:1116318/149‑1 (MQ=255)
                                                                                                                                                                                                                                tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                                                                                                                                                       >  8:1226906/1‑149 (MQ=255)
                                                                                                                                                                                                                                tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                                                                                                                                                       >  6:1155044/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTcc                                                                                                                                                                     >  5:1352001/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGAtt                                                                                                                                                            >  7:416511/1‑146 (MQ=255)
                                                                                                                                                                                                                                              aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGAtt                                                                                                                                                            <  8:416511/146‑1 (MQ=255)
                                                                                                                                                                                                                                               aaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                                                                                                                                                        >  6:849704/1‑149 (MQ=255)
                                                                                                                                                                                                                                                aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaa                                                                                                                                                                                                                                                        <  5:378244/53‑1 (MQ=255)
                                                                                                                                                                                                                                                aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                                                                                                                                                              >  5:1424233/1‑126 (MQ=255)
                                                                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCa                                                                                                                                                                    >  7:455848/1‑131 (MQ=255)
                                                                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCa                                                                                                                                                                    <  8:455848/131‑1 (MQ=255)
                                                                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                                                                                                                                                  >  6:1591062/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCACACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                                                                                                                                                  >  5:947103/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      aaGAAAAAACCAACCCTTATGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcg                                                                                                                                                 >  7:992203/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAt                                                                                                                                             >  7:717918/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           aaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt                                                                                                                                            <  5:1026508/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATc                                                                                                                                                                                                                              >  8:33500/1‑65 (MQ=255)
                                                                                                                                                                                                                                                                caaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt                                                                                                                                       <  8:20212/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                caaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt                                                                                                                                       >  6:1718741/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                caaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt                                                                                                                                       >  8:853384/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  accCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAg                                                                                                                                     <  5:1627517/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCAc                                                                  <  4:560743/211‑1 (MQ=255)
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                 
CGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTG‑ACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACTGACAAATGACCGCTTAACGTCGGGGTAAACGATGACCTTATACCAGCCTGATAAACGCCACTGGTTCCAGCCACATCATTATTAAATTTGCCATCGTCATTAACTTTCACCTGGTTAGAATCGGC  >  NC_000913/2286050‑2286587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑