Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,311,619 A→G 6.0% intergenic (‑85/+27) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,311,6190AG 6.0% 970.3 / 33.0 294intergenic (‑85/+27)apbE/ompCputative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (142/133);  new base G (11/7);  total (154/140)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.87e-02

Only 100 of 300 total aligned reads displayed.

TTCTTTAAGTTCGGCGCTGCGTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACT  >  NC_000913/2311346‑2311890
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                               
                                              gCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAcc                                                                                                                                                                                                                                  <  2:644498/275‑1 (MQ=255)
                                                               gtaaGTTTTGCCTTCCAGTACAGTAGATTCGGTAGCTGGGTGTTTGGCGGGTTGTTCTGTTTTCTCGCAACCAACAAAGAAGAGCGCGGAAACCAGCAATGCGTGGCGGGTAAAGCTTATTTCCACACGGGATTACCCTCAGGCGAAGGGTCGCAAGAGTCGACCAAAGAACTAAGTTTGGACGCTGAAAACAAACAAAAAAGGGCCCGGAGGCCCTTTGTTCGATATCAATAGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGCTGTTAt                                                                                                                                                                                                                 <  4:613913/272‑1 (MQ=255)
                                                                       tGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGAGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCg                                                                                                                                                                                                                                                                    >  4:390062/1‑216 (MQ=255)
                                                                                                             ggCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                                                                                                                    >  2:547655/1‑275 (MQ=255)
                                                                                                                          ttttGATTGAAAACAACAAAGAAGGGGGATGCTGGCAGGCGTGCAACTCGGGTAAAGCCTATTTCCATCCGGTATTATCCTAAGGCGAAGGGTCGTAAGTGTAAACCAAAAAACGTAGTTTGTACGCTGCAAAAAATGGAAAAAGGGCACGCAGGCCCTTTGTTCGATATCACTAGAGGTTAGAACTGGTAAACCAGACCCAGAGCTACGATTTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                                                                                                                       <  4:161001/275‑1 (MQ=255)
                                                                                                                                tCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAgg                                                                                                                                                                                                                                                                               >  6:1517788/1‑149 (MQ=255)
                                                                                                                                  gCAACCAACAAGGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGcc                                                                                                                                                                                                                                                                             >  7:1329519/1‑149 (MQ=255)
                                                                                                                                       caacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCTTGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCtttt                                                                                                                                                                                                                                                                        <  6:1042950/149‑2 (MQ=255)
                                                                                                                                        aacaaAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCTGCGGGCCCttttt                                                                                                                                                                                                                                                                       <  8:1590640/149‑3 (MQ=255)
                                                                                                                                            aagaagAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGa                                                                                                                                                                                                                                                                   >  7:1187708/1‑149 (MQ=255)
                                                                                                                                             agaagaGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGAGCCCGCAGGCCCTTTGTTCGat                                                                                                                                                                                                                                                                  >  5:1303340/1‑149 (MQ=255)
                                                                                                                                                  agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                                                                                                               >  2:492008/1‑275 (MQ=255)
                                                                                                                                                  agCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                                                                                                               >  2:153343/1‑275 (MQ=255)
                                                                                                                                                    cgcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                                                                                                                                                                                               >  4:736397/1‑145 (MQ=255)
                                                                                                                                                    cgcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATc                                                                                                                                                                                                                                                           <  5:596757/149‑1 (MQ=255)
                                                                                                                                                    cgcgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGa                                                                                                                                                                                                                                                  >  3:26726/1‑158 (MQ=255)
                                                                                                                                                      cgGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                                                                                                                                                                                                             <  2:701822/177‑1 (MQ=255)
                                                                                                                                                       ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACGCCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgag                                                                                                                                                                                                                                                        <  5:56228/149‑1 (MQ=255)
                                                                                                                                                       ggCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGa                                                                                                                          <  4:547651/275‑1 (MQ=255)
                                                                                                                                                        gCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCgaga                                                                                                                                                                                                                                                       >  8:815146/1‑149 (MQ=255)
                                                                                                                                                         cagccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGaa                                                                                                                         >  2:202025/1‑274 (MQ=255)
                                                                                                                                                           gccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTa                                                                                                                                                                                                                                                    >  5:793191/1‑149 (MQ=255)
                                                                                                                                                            ccagcAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAg                                                                                                                                                                                                                                                   >  6:713120/1‑149 (MQ=255)
                                                                                                                                                              agcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGaa                                                                                                                                                                                                                                                 <  7:1627444/149‑1 (MQ=255)
                                                                                                                                                               gcagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAAc                                                                                                                                                                                                                                                >  5:268260/1‑149 (MQ=255)
                                                                                                                                                                cagTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCAAGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACt                                                                                                                                                                                                                                               <  7:1452966/149‑1 (MQ=255)
                                                                                                                                                                  gTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                                                                                                                                                             <  6:837541/149‑1 (MQ=255)
                                                                                                                                                                     ccACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaa                                                                                                                                                                                                                                          <  5:757659/149‑1 (MQ=255)
                                                                                                                                                                       aCGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                                                                                                                                                                                                        >  7:753958/1‑149 (MQ=255)
                                                                                                                                                                           gggTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGa                                                                                                                                                                                                                                    <  8:1329519/149‑1 (MQ=255)
                                                                                                                                                                             gTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGAcc                                                                                                                                                                                                                                  >  8:75285/1‑149 (MQ=255)
                                                                                                                                                                              tAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTGGCAAGAGGACACCAAAGAACTGAGTTTGTACGCTGAAAACAATGAAAAAACGGCCCGCAGGCCCTTTGTTCGATATGAATCGAGATTAGAACTGGGAAACCAGAgcc                                                                                                                                                                                                                                 >  8:1409571/1‑149 (MQ=255)
                                                                                                                                                                              tAAAGCTTATTTCCATACGTGATTAGCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACcaacatcaac                                                                                                 <  1:15198/277‑1 (MQ=255)
                                                                                                                                                                                  gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                                                                                                                                                                                                             >  7:1379903/1‑149 (MQ=255)
                                                                                                                                                                                  gCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCagag                                                                                                                                                                                                                             >  7:675487/1‑149 (MQ=255)
                                                                                                                                                                                        ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGa                                                                                                                                                                                                                       <  5:907424/149‑1 (MQ=255)
                                                                                                                                                                                        ttCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGa                                                                                                                                                                                                                       <  6:895399/149‑1 (MQ=255)
                                                                                                                                                                                         tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                                                                                                                                                      <  5:601991/149‑1 (MQ=255)
                                                                                                                                                                                         tCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGAt                                                                                                                                                                                                                      <  8:380552/149‑1 (MQ=255)
                                                                                                                                                                                          ccATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATg                                                                                                                                                                                                                     <  5:1600097/149‑1 (MQ=255)
                                                                                                                                                                                             tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATc                                                                                                                                                                                                                                                               >  2:339753/1‑104 (MQ=255)
                                                                                                                                                                                             tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                                                                                                                                                  >  7:332812/1‑149 (MQ=255)
                                                                                                                                                                                             tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                                                                                                                                                  <  8:1674976/149‑1 (MQ=255)
                                                                                                                                                                                             tACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAATGTAGGTGGACATGTTTTTGTTTAAGTAGTAGGTAGCCCCAACATCAACATATTTCAGGaaa                                                                                    >  4:213124/1‑273 (MQ=255)
                                                                                                                                                                                                 tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAGGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTa                                                                                                                                                                                                                  <  5:190142/145‑1 (MQ=255)
                                                                                                                                                                                                 tGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATc                                                                                                                                                                                                                                                           <  4:339749/104‑1 (MQ=255)
                                                                                                                                                                                                    ttATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAgtgt                                                                                                                                                                                                           <  7:217685/149‑1 (MQ=255)
                                                                                                                                                                                                       tCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGa                                                                                                                                                                                                        <  7:122429/149‑1 (MQ=255)
                                                                                                                                                                                                         cacATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCTTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAc                                                                                                                                                                                                                                        >  8:1212815/3‑115 (MQ=255)
                                                                                                                                                                                                         cTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATg                                                                                                                                                                                                      >  6:1413117/1‑149 (MQ=255)
                                                                                                                                                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                                                                                                                                     <  6:1446270/149‑1 (MQ=255)
                                                                                                                                                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                                                                                                                                     >  6:198397/1‑149 (MQ=255)
                                                                                                                                                                                                          tCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGc                                                                                                                                                                                                     >  5:1047260/1‑149 (MQ=255)
                                                                                                                                                                                                              gCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGc                                                                                                                                                                                                 >  6:1302356/1‑149 (MQ=255)
                                                                                                                                                                                                                 aaCGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTa                                                                                                                                                       <  1:248668/188‑1 (MQ=255)
                                                                                                                                                                                                                     ttCGCAAGAGTACACCAAAAAACTGAGTTTGTAAGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGAccc                                                            <  2:416104/274‑1 (MQ=255)
                                                                                                                                                                                                                     gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTgg                                                                                                                                                                                                                                             <  4:774675/98‑1 (MQ=255)
                                                                                                                                                                                                                     gTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                                                                                                                                                          <  6:526228/149‑1 (MQ=255)
                                                                                                                                                                                                                      tCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGatat                                                                                                                                                                                                                                                                >  3:241536/1‑78 (MQ=255)
                                                                                                                                                                                                                        gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGa                                                                                                                                                                                          >  7:974606/1‑146 (MQ=255)
                                                                                                                                                                                                                        gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                                                                                                                                       <  6:575134/149‑1 (MQ=255)
                                                                                                                                                                                                                        gCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTg                                                                                                                                                                                       >  6:787710/1‑149 (MQ=255)
                                                                                                                                                                                                                          aaGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGaa                                                                                                                                                                                     >  7:90783/1‑149 (MQ=255)
                                                                                                                                                                                                                              gTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGt                                                                                                                                                                                                                                            >  1:55389/1‑90 (MQ=255)
                                                                                                                                                                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                                                                                                                               <  8:1646312/149‑1 (MQ=255)
                                                                                                                                                                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                                                                                                                               <  7:555014/149‑1 (MQ=255)
                                                                                                                                                                                                                                acacCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTAcc                                                 >  2:52478/1‑275 (MQ=255)
                                                                                                                                                                                                                                acacAAAAAAACTGAGTTTGTCCGCTGAAAACAAGGAAAAAAGGGCCCGCAGGCCATTTGTGCGATATCAATCGAGATTAGAACTTGTAAACCAGCCCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGtt                                                                                                                                                                               <  5:31897/149‑1 (MQ=255)
                                                                                                                                                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGatat                                                                                                                                                                                                                                                                <  5:1430486/66‑1 (MQ=255)
                                                                                                                                                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                                                                                                                             >  6:30034/1‑149 (MQ=255)
                                                                                                                                                                                                                                  acCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgt                                                                                                                                                                             >  6:646369/1‑149 (MQ=255)
                                                                                                                                                                                                                                   ccAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGt                                                                                                                                               <  2:738636/178‑1 (MQ=255)
                                                                                                                                                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTaaa                                                                                                                                                                                                                                         <  8:563557/87‑1 (MQ=255)
                                                                                                                                                                                                                                    cAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcg                                                                                                                                                                           >  6:1639059/1‑149 (MQ=255)
                                                                                                                                                                                                                                     aaaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgt                                                                                                                                                                          >  7:1084126/1‑149 (MQ=255)
                                                                                                                                                                                                                                      aaaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTgtcgtc                                                                                                                                                                         <  6:1140552/149‑1 (MQ=255)
                                                                                                                                                                                                                                       aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTa                                                                                                                                                                                                                                           >  4:678726/1‑82 (MQ=255)
                                                                                                                                                                                                                                       aaaaCTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTcc                                                                                                                                                                        <  5:1517788/149‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAAcc                                                                                                                                                                                                                                       <  2:678731/82‑1 (MQ=255)
                                                                                                                                                                                                                                           cTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCcagca                                                                                                                                                                    <  7:1665748/149‑1 (MQ=255)
                                                                                                                                                                                                                                               gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                                                                                                                                                >  8:937258/1‑149 (MQ=255)
                                                                                                                                                                                                                                               gTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGtt                                                                                                                                                                >  5:1470418/1‑149 (MQ=255)
                                                                                                                                                                                                                                                tttGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTg                                                                                                                                                               >  5:1163973/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aCGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGAtttt                                                                                                                                                          <  7:904569/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       gCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGt                                                                                                                                                        <  8:702369/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           aaaaCAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTc                                                                                                                                                    >  7:805367/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  tGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTTTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAg                                                                                                                                             >  5:1678663/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   gAACAAAGGGCCTGCGGGCCCTTTTTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAgg                                                                                                                                            >  6:397602/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     aaaaaGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACg                                                                                                                                                <  6:800455/143‑1 (MQ=255)
                                                                                                                                                                                                                                                                       aaaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGa                                                                                                                                        >  6:565554/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        aaGGGCCTGCGGGCCCTTTGTTCTATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                                                                                                                           <  7:1161464/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aaGGGCCTGCGGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCTGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGt                                                                                                                                           >  8:1161464/1‑145 (MQ=255)
                                                                                                                                                                                                                                                                         aGGGCCCGAAGGCCCTGTGTTCGATATCAATCGAGAATAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGt        <  4:103838/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ggCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCg      <  2:302212/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tcAGGCCCTTTTTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGTGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGCTTGTCGTCCAGCAGGTAGATTTTGCAGTCATCGTAGGTGGACTTGttttg                                                                                                                               <  8:48884/148‑2 (MQ=255)
                                                                                                                                                                                                                                                                                gCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTACACCAGACCCAGAGCTACGATATTATCAGCGCTGATGCCAGCGTCGCGAGTGAACTGGTTGTCGTCCAGCAGGTTGTTTTTGTAGTCAACGTAGGTGGACATGttttt                                                                                                                               <  7:1409571/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGttttt                                                                                                                               <  7:1592506/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                                                                                             <  5:50568/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                                                                                             <  8:27520/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  aGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTttgt                                                                                                                             <  8:1084126/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                               
TTCTTTAAGTTCGGCGCTGCGTTTGGCGTCAATGCCCGGGATGCTGGCACGCCAGAAGGTACCCATAGTTTTGCCTTCAAGAACGGTAACTTCAGTAGCGTGGGTTTTGGCGGGTTGTGGTTTTTGATCGCAACCAACAAAGAAGAGCGCGGCAGCCAGCAGTGCCACGCGGGTAAAGCTTATTTCCATACGTGATTATCCTCATGCGAACGGTCGCAAGAGTACACCAAAAAACTGAGTTTGTACGCTGAAAACAATGAAAAAAGGGCCCGCAGGCCCTTTGTTCGATATCAATCGAGATTAGAACTGGTAAACCAGACCCAGAGCTACGATGTTATCAGTGTTGATGCCAGCGTCACGAGTGAACTGGTTGTCGTCCAGCAGGTTGATTTTGTAGTCAACGTAGGTGGACATGTTTTTGTTGAAGTAGTAGGTAGCACCAACATCAACATATTTCAGGATATCTTCGTCGTCGTAGCCACGACCCAGGTTTTTACCTTTAGACTGCAGGTAAGCCAGGGACGGACGCAGACCGAAGTCGAACT  >  NC_000913/2311346‑2311890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: