Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,314,801 G→T 5.5% Q438H (CAG→CAT) ‡ rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,314,8010GT 5.5% 904.9 / 27.1 256Q438H (CAG→CAT) ‡rcsDphosphotransfer intermediate protein in two‑component regulatory system with RcsBC
Reads supporting (aligned to +/- strand):  ref base G (127/114);  new base T (7/8);  total (134/122)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.76e-01

Only 100 of 260 total aligned reads displayed.

CGAATTGCGCATTTTACGGGCAATCAATGAAGAGATAGTCTCACTGCTGCCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTG  >  NC_000913/2314531‑2315074
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                 
cGAATTGCGCATTTTACGGGCAATCAATGAAGAGATAGTCTCACTGCTGCCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCCCTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACCAGCTGGATGAGATCCGAATGTTCCGCAGCCAGGTCGCGCCGCGGCCACAAATTTTAATTATTAGCGATCAGGaac                                                                                                                                                                                                                                                                               >  4:520771/1‑273 (MQ=255)
                                 gaTAGTCTCACTGCTGCCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCCAAACATCACAACCATTGCGGAACAGCATCAGGGGATTATTAAGGCGACGGTCAATAACGCGCTGTCTGAGATCCGCATGTTCCGCATCACGGTCGGGCCGCCCAACCCAATTTTCATTATTAGCTACCAGGCTCGCGCAGTGCTGCGAACCAAACAACTCAAACAgg                                                                                                                                                                                                                                              >  4:75233/1‑275 (MQ=255)
                                     gTCTCACTGCTGCCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCGCCACCATGGCGGAACAGCATCAGAGGATTATTCAGGCGACGATAAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGGTCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGACGCa                                                                                                                                                                                                                                          <  2:309200/275‑1 (MQ=255)
                                               tgcCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                                                                                                                                                                                                 <  2:691565/274‑1 (MQ=255)
                                                                gttacagagaaGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCAGTTGCTGACGCATTTGAATCTGAAAAACATCACCACCATGGCGGAACAGCAGCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAgg                                                                                                                                                                                                                <  4:192763/264‑1 (MQ=255)
                                                                                       cACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTCTGAGATCCGCATGTTCCGCAGCCAGGTCGCTCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAAAAAATCAAGCAGGCGCCGCGTCGGTATGAGAAAACCCAACAGGGAGCGATGATCTTTACTaaaaaac                                                                                                                                                                                        >  4:155688/1‑273 (MQ=255)
                                                                                                      cAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgc                                                                                                                                                                                                                                                                             <  3:146767/175‑1 (MQ=255)
                                                                                                      cAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCCATGCGCTGa                                                                                                                                                                         >  2:183902/1‑275 (MQ=255)
                                                                                                      cAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGGATGAGAAAAACCAGCAGGGGCGGATGATCGTTATGAAAAACATTGGCAGTGCGCTGa                                                                                                                                                                         >  4:361113/1‑275 (MQ=255)
                                                                                                          aTTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaa                                                                                                                                                                                                                                                                <  4:683473/184‑1 (MQ=255)
                                                                                                                                 tttGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgcg                                                                                                                                                                                                                                                                            >  7:708800/1‑149 (MQ=255)
                                                                                                                                 tttGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATcgcg                                                                                                                                                                                                                                                                            >  7:913964/1‑149 (MQ=255)
                                                                                                                                 tttGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATc                                                                                                                                                                                                                                                                               <  8:64810/146‑1 (MQ=255)
                                                                                                                                   tGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaa                                                                                                                                                                                                                                                                          <  7:1240550/149‑1 (MQ=255)
                                                                                                                                   tGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaa                                                                                                                                                                                                                                                                          <  8:35560/149‑1 (MQ=255)
                                                                                                                                   tGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCCTGATCGCGaa                                                                                                                                                                                                                                                                          <  5:1533431/149‑1 (MQ=255)
                                                                                                                                      aTCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTg                                                                                                                                                                                                                                                                       >  5:921821/1‑149 (MQ=255)
                                                                                                                                       tCTGCAAAACATCACCACCATGGCGGAACTGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGc                                                                                                                                                                                                                                                                      <  5:993464/149‑1 (MQ=255)
                                                                                                                                         tGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGCAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAACGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCt                                                                                                                                      >  2:270868/1‑275 (MQ=255)
                                                                                                                                         tGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTg                                                                                                                                                                                                                                                                    <  8:604895/149‑1 (MQ=255)
                                                                                                                                         tGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTg                                                                                                                                                                                                                                                                    <  7:345329/149‑1 (MQ=255)
                                                                                                                                          gCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGc                                                                                                                                                                                                                                                                      >  2:579030/1‑146 (MQ=255)
                                                                                                                                              aaCATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTaaa                                                                                                                                                                                                                                                               >  5:330944/1‑149 (MQ=255)
                                                                                                                                               aCATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAAc                                                                                                                                                                                                                                                              >  6:15744/1‑149 (MQ=255)
                                                                                                                                                  tCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAg                                                                                                                                                                                                                                                           >  7:415053/1‑149 (MQ=255)
                                                                                                                                                   caccacCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGa                                                                                                                                                                                                                                                          >  7:1009214/1‑149 (MQ=255)
                                                                                                                                                    accaccATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaa                                                                                                                                                                                                                                                         >  6:225968/1‑149 (MQ=255)
                                                                                                                                                     ccaccaTGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaaa                                                                                                                                                                                                                                                        <  8:415053/149‑1 (MQ=255)
                                                                                                                                                          aTGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCaa                                                                                                                                                                                                                                                   <  8:581851/149‑1 (MQ=255)
                                                                                                                                                          aTGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCaa                                                                                                                                                                                                                                                   <  8:708800/149‑1 (MQ=255)
                                                                                                                                                          aTGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCaa                                                                                                                                                                                                                                                   <  5:1591396/149‑1 (MQ=255)
                                                                                                                                                          aTGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGa                                                                                                                     <  2:520775/275‑1 (MQ=255)
                                                                                                                                                               ggAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAgg                                                                                                                                                                                                                                              >  5:678386/1‑149 (MQ=255)
                                                                                                                                                                 aaCAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAg                                                                                                                                                                                                                                                                                   >  8:1011022/1‑110 (MQ=255)
                                                                                                                                                                     gCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGc                                                                                                                                                                                                                                        >  8:1317647/1‑149 (MQ=255)
                                                                                                                                                                       aTCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGt                                                                                                                                                                                                                                      >  7:1338584/1‑149 (MQ=255)
                                                                                                                                                                       aTCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGt                                                                                                                                                                                                                                      >  8:18980/1‑149 (MQ=255)
                                                                                                                                                                            gggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTa                                                                                                                                                                                                                                 <  7:73404/149‑1 (MQ=255)
                                                                                                                                                                             ggATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTAt                                                                                                                                                                                                                                <  6:945400/149‑1 (MQ=255)
                                                                                                                                                                              gATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaaa                                                                                                                                                                                                                                                        <  7:1603333/124‑1 (MQ=255)
                                                                                                                                                                                ttattCAGGCGACGATCAATAACGAGCTGTCTGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcag                                                                                                                                                                                                                    >  4:780577/1‑158 (MQ=255)
                                                                                                                                                                                ttattCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTg                                                                                                                                                                                                                                                                    >  7:1030838/1‑110 (MQ=255)
                                                                                                                                                                                ttattCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAACGCAAACAACTGGCGAATCAg                                                                                               >  2:672645/1‑275 (MQ=255)
                                                                                                                                                                                 tattCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATgaga                                                                                                                                                                                                                            <  5:1699477/149‑1 (MQ=255)
                                                                                                                                                                                   ttCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaa                                                                                                                                                                                                                          >  6:1497532/1‑149 (MQ=255)
                                                                                                                                                                                   ttCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaa                                                                                                                                                                                                                          >  7:878563/1‑149 (MQ=255)
                                                                                                                                                                                   ttCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGaaa                                                                                                                                                                                                                          >  6:46729/1‑149 (MQ=255)
                                                                                                                                                                                      aGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAAc                                                                                                                                                                                                                       >  7:1529235/1‑149 (MQ=255)
                                                                                                                                                                                        gCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTg                                                                                                                                                                                                                                                                    <  7:553569/102‑1 (MQ=255)
                                                                                                                                                                                        gCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACca                                                                                                                                                                                                                     <  6:1190448/149‑1 (MQ=255)
                                                                                                                                                                                          gacgaTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagc                                                                                                                                                                                                                   >  8:1343976/1‑149 (MQ=255)
                                                                                                                                                                                              aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAggg                                                                                                                                                                                                               >  5:821168/1‑149 (MQ=255)
                                                                                                                                                                                              aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAggg                                                                                                                                                                                                               >  8:1244860/1‑149 (MQ=255)
                                                                                                                                                                                              aTCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAggg                                                                                                                                                                                                               <  5:274949/149‑1 (MQ=255)
                                                                                                                                                                                                cAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGc                                                                                                                                                                                                             <  6:730691/149‑1 (MQ=255)
                                                                                                                                                                                                   tAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGga                                                                                                                                                                                                          >  7:496078/1‑149 (MQ=255)
                                                                                                                                                                                                     aCGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTTAAGCAGGCGCAGCGTCTGTATGAGAAAATCCAGCAGGGGCGgatg                                                                                                                                                                                                        >  8:263443/1‑149 (MQ=255)
                                                                                                                                                                                                       gAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatgat                                                                                                                                                                                                      <  8:1088834/149‑1 (MQ=255)
                                                                                                                                                                                                       gAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatgat                                                                                                                                                                                                      >  8:739873/1‑149 (MQ=255)
                                                                                                                                                                                                         gCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTCGTAAACAAGAAACTCAAGCAGGCTCAGCGTCGGTCTGAGCAAAACCAGCAGGGNCGGATGATCTTTATGAAAAACATTGGAGATGCGCCGAAAGAACCCGCAAAGTCCCCGGCGGAGAGCAGGGAGAAACTAAACGCACCGGAAAGCAAACAACTggtgtnannagta                                                                                            >  3:190419/1‑242 (MQ=255)
                                                                                                                                                                                                          cTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCtt                                                                                                                                                                                                   <  7:18980/149‑1 (MQ=255)
                                                                                                                                                                                                           tGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAgcgc                                                                                                                                          >  4:23138/1‑205 (MQ=255)
                                                                                                                                                                                                             tATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATTATCTTTAt                                                                                                                                                                                                <  6:558639/149‑1 (MQ=255)
                                                                                                                                                                                                                 agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCttt                                                                                                                                                                                                  >  6:967686/1‑143 (MQ=255)
                                                                                                                                                                                                                 agaTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCg                                                                                                                                                                                  <  4:654328/159‑1 (MQ=255)
                                                                                                                                                                                                                   aTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATc                                                                                                                                                                                                     <  6:1367901/138‑1 (MQ=255)
                                                                                                                                                                                                                   aTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATc                                                                                                                                                                                                     >  5:1367901/1‑138 (MQ=255)
                                                                                                                                                                                                                   aTCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGaaaaa                                                                                                                                                                                          <  6:1259562/149‑1 (MQ=255)
                                                                                                                                                                                                                     ccGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaaa                                                                                                                                                                                                                                                        >  7:1642108/1‑85 (MQ=255)
                                                                                                                                                                                                                     ccGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGaaa                                                                                                                                                                                                                                                        <  8:1642108/85‑1 (MQ=255)
                                                                                                                                                                                                                     ccGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGGTGAAATACCGt                                                          >  2:229294/1‑275 (MQ=255)
                                                                                                                                                                                                                     ccGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGt                                                          <  2:155690/275‑1 (MQ=255)
                                                                                                                                                                                                                       gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCt                                                                                                                                                                                                                                                                     >  2:330629/1‑70 (MQ=255)
                                                                                                                                                                                                                       gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGt                                                                                                                                                                                                                                      >  2:428603/1‑101 (MQ=255)
                                                                                                                                                                                                                       gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACcagc                                                                                                                                                                                                                   >  1:183671/1‑120 (MQ=255)
                                                                                                                                                                                                                       gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACAtt                                                                                                                                                                                      <  6:710577/149‑1 (MQ=255)
                                                                                                                                                                                                                       gCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGt                                                                                                                                                          <  1:277661/177‑1 (MQ=255)
                                                                                                                                                                                                                         aTGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATTAAAAACATTgg                                                                                                                                                                                    <  7:985885/149‑1 (MQ=255)
                                                                                                                                                                                                                          tGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGc                                                                                                                                                                                   <  7:1260284/149‑1 (MQ=255)
                                                                                                                                                                                                                           gTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGt                                                                                                                                                                                                                                  <  4:428599/101‑1 (MQ=255)
                                                                                                                                                                                                                            gaccGCAGCCAGAGCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACAt                                                <  1:102563/276‑1 (MQ=255)
                                                                                                                                                                                                                                gCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTc                                                                                                                                                                                                                                                     <  6:88409/77‑1 (MQ=255)
                                                                                                                                                                                                                                     cAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATga                                                                                                                                                                                                                              <  7:1108533/95‑1 (MQ=255)
                                                                                                                                                                                                                                      aGGTCGCGCCGCGCACACAAATTTTCATTATTCTCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatgat                                                                                                                                                                                                      >  5:524475/1‑118 (MQ=255)
                                                                                                                                                                                                                                      aGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGgatgat                                                                                                                                                                                                      <  6:524475/118‑1 (MQ=255)
                                                                                                                                                                                                                                      aGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGaaa                                                                                                                                                                       <  5:1497532/149‑1 (MQ=255)
                                                                                                                                                                                                                                       ggTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTAt                                                                                                                                                                                                                                >  5:635460/1‑91 (MQ=255)
                                                                                                                                                                                                                                         tCGCGCCGCGCAGACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGaa                                                                                                                                                                    <  5:779917/149‑1 (MQ=255)
                                                                                                                                                                                                                                             gccgcgcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCg                                                                                                                                                                >  5:229405/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  gcACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGAACTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTgg                             >  2:645926/1‑275 (MQ=255)
                                                                                                                                                                                                                                                    acacaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCt                                                                                                                                                                                                    <  5:480613/106‑1 (MQ=255)
                                                                                                                                                                                                                                                    acacaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTc                                                                                                                                                         <  5:950380/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      acaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACTCGCACAGTccc                                                                                                                                                       >  8:1485636/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      acaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTccc                                                                                                                                                       >  5:374926/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      acaAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTccc                                                                                                                                                       >  8:911485/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          aTTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGc                                                                                                                                                   >  6:373678/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                attattCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGagag                                                                                                                                             >  7:291253/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                attattCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGagag                                                                                                                                             >  7:765929/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                attattCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTg                                                                          >  2:727660/1‑216 (MQ=255)
                                                                                                                                                                                                                                                                            cAGGATCGCGAAGGGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGt   <  2:656446/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                 
CGAATTGCGCATTTTACGGGCAATCAATGAAGAGATAGTCTCACTGCTGCCGCTCGGCCTGCTGGTTCACGATCAGGAATCGAACCGCACTGTCATAAGTAACAAAATTGCCGATCATTTGCTGCCGCATTTGAATCTGCAAAACATCACCACCATGGCGGAACAGCATCAGGGGATTATTCAGGCGACGATCAATAACGAGCTGTATGAGATCCGCATGTTCCGCAGCCAGGTCGCGCCGCGCACACAAATTTTCATTATTCGCGATCAGGATCGCGAAGTGCTGGTAAACAAGAAACTCAAGCAGGCGCAGCGTCTGTATGAGAAAAACCAGCAGGGGCGGATGATCTTTATGAAAAACATTGGCGATGCGCTGAAAGAACCCGCACAGTCCCTGGCGGAGAGCGCGGCTAAACTCAACGCCCCGGAAAGCAAACAACTGGCGAATCAGGCAGATGTGCTGGTGCGGCTGGTTGATGAAATACAGTTAGCGAACATGCTTGCGGATGATAGCTGGAAAAGTGAGACGGTGCTGTTCTCCGTG  >  NC_000913/2314531‑2315074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑