Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,462,677 A→T 5.3% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,6770AT 5.3% 940.5 / 30.7 270intergenic (+31/‑335)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (118/130);  new base T (7/7);  total (129/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.74e-02

Only 100 of 276 total aligned reads displayed.

CTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTT  >  NC_000913/2462418‑2462946
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
   tgatgaCAGCCCAGTTCCTGGAATGAATCGTTCTATTTCCATTCCGGACCGGGACCGTTTCTGGTTGGTGGAAGGAACGACTTACGCATTTAATAAAGATGCTTCAGTCAAAGTGGGGGTTTCTGAGATGTACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAAc                                                                                                                                                                                                                                                              <  4:598436/274‑1 (MQ=255)
                      gCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGAAGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                                                                                                                                            >  2:217273/1‑273 (MQ=255)
                            aaTCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCCCCGAAAGTAgtgt                                                                                                                                                                                                                                     >  2:58233/1‑274 (MQ=255)
                                 ttCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCGGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGACGGCCCATACCCGTTCGAGTCTGGAGGTAAAGCCTGGATGTTCGGTACTAACTTTAACTCCGCGTTCTGAAAACGCGTTCGCCTGGACAACGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCCCACGTAGAGTACTCAt                                                                                                                                                                                                                               >  4:355048/1‑275 (MQ=255)
                                  tttaccaccATTCCGGGCCAGGTCCGTTTCTGGNGGAGTGATGGTACGATTTACGACATNAATAAAGCTGATACATTCGGAGGTGGTGTTTCTTATAGTCACGGTAAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTNGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          <  3:182247/272‑1 (MQ=255)
                                            ttCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGCCTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCCCGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGCAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCTTTCTTATAACGCGTTCGCCTTGATAACGTCACCTGCCTAGCCGGTGACTTTAACTCCCCCACTTCACCGACAGTAGTTTCCTAATAGCTTACCTCCTGac                                                                                                                                                                                                                >  1:200914/1‑278 (MQ=255)
                                             tacgaccaaGGAACGTTTCTGGCTGAGTGCAGGTACGGATTACGCATTTAATAAAAATGCTTCAGTGGACGTTGGTGTTTCTTGTATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcctcct                                                                                                                                                                                                                   <  4:169794/267‑1 (MQ=255)
                                                                                    ttACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCa                                                                                                                                                                             <  2:355052/274‑1 (MQ=255)
                                                                                                        cTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcaca                                                                                                                                                        >  4:326472/1‑275 (MQ=255)
                                                                                                            aGTCGACGTTGGTGTTTCTTATATGCACGGTAAGGGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACa                                                                                                                                                    <  2:626934/275‑1 (MQ=255)
                                                                                                               cGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCa                                                                                                                                                                                                                                                                                <  6:1718158/149‑1 (MQ=255)
                                                                                                               cGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCa                                                                                                                                                                                                                                                                                <  7:1199055/149‑1 (MQ=255)
                                                                                                                gACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCAt                                                                                                                                                                                                                                                                               >  5:788164/1‑149 (MQ=255)
                                                                                                                 aCGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATa                                                                                                                                                                                                                                                                              <  7:1580623/149‑1 (MQ=255)
                                                                                                                    ttGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTCCTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCa                                                                                                                                                                                                                                                                          >  8:606386/1‑149 (MQ=255)
                                                                                                                        tgtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTg                                                                                                                                                                                                                                                                      >  5:900601/1‑149 (MQ=255)
                                                                                                                         gtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGTTAAAGTCACCTGCTATGCAGGTGa                                                                                                                                                                                                                                                                     <  7:96960/149‑1 (MQ=255)
                                                                                                                         gtTTCTTATATGAACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                                                                                                                                                     <  7:606386/149‑1 (MQ=255)
                                                                                                                          tttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGAc                                                                                                                                                                                                                                                                    <  6:725720/149‑1 (MQ=255)
                                                                                                                          tttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGAc                                                                                                                                                                                                                                                                    >  6:981539/1‑149 (MQ=255)
                                                                                                                          tttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGAc                                                                                                                                                                                                                                                                    >  6:408660/1‑149 (MQ=255)
                                                                                                                            tCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACtt                                                                                                                                                                                                                                                                  <  7:988985/149‑1 (MQ=255)
                                                                                                                                 tatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGt                                                                                                                                                                                                               >  2:505093/1‑195 (MQ=255)
                                                                                                                                    gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                                                                                                                                          <  6:749376/149‑1 (MQ=255)
                                                                                                                                    gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                                                                                                                                          >  8:652144/1‑149 (MQ=255)
                                                                                                                                     cACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTg                                                                                                                                                                                                           <  4:505089/195‑1 (MQ=255)
                                                                                                                                      aCGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTc                                                                                                                                                                                                                                 <  2:261821/172‑1 (MQ=255)
                                                                                                                                        ggTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAACCCTGAAGGGAAAGGCATATTTTCAg                                                                                                                         >  4:234438/1‑274 (MQ=255)
                                                                                                                                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGa                                                                                                                                                                                                                                                                     >  6:260055/1‑129 (MQ=255)
                                                                                                                                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                                                                                                                                                 <  5:1711155/149‑1 (MQ=255)
                                                                                                                                                 gTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                                                                                                                                                 <  6:885944/145‑1 (MQ=255)
                                                                                                                                                  tGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                                                                                                                                            <  7:1406967/149‑1 (MQ=255)
                                                                                                                                                      aaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGa                                                                                                                                                                                                                                              >  7:303232/1‑143 (MQ=255)
                                                                                                                                                        ttACCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTGCACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAgtg                                                                                                                                                                                                                                      >  8:1437613/1‑149 (MQ=255)
                                                                                                                                                           aCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTcc                                                                                                                                                                                                                                   >  7:475916/1‑149 (MQ=255)
                                                                                                                                                                ggCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCAtt                                                                                                                                                                                                                              <  8:1515215/149‑1 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                                                                                                                                                     >  8:1557064/1‑107 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                                                                                                                                                 <  8:24907/111‑1 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                                                                                                                                                 >  7:24907/1‑111 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                                                                                                                                           >  5:374037/1‑149 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                                                                                                                                           <  6:1554870/149‑1 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          <  7:550583/149‑1 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          >  8:1023261/1‑149 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          <  5:266481/149‑1 (MQ=255)
                                                                                                                                                                     aTACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTa                                                                                                                                                                                                                         >  7:185938/1‑149 (MQ=255)
                                                                                                                                                                      tACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAc                                                                                                                                                                                                                        <  8:812501/149‑1 (MQ=255)
                                                                                                                                                                       aCCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcc                                                                                                                                                                                                                       >  5:472225/1‑149 (MQ=255)
                                                                                                                                                                        ccAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                                                                                                                                                 >  5:993824/1‑122 (MQ=255)
                                                                                                                                                                         cAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagta                                                                                                                                                                                                                                         <  7:855154/129‑1 (MQ=255)
                                                                                                                                                                          aGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACtt                                                                                                                                                                                                                                                                  <  8:287916/103‑1 (MQ=255)
                                                                                                                                                                           gTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACNGAAAGTANTGTNCTCATTNCTTACCTCCTGAGTTTTGCAAAc                                                                                                                                                                                                      >  3:39859/1‑162 (MQ=255)
                                                                                                                                                                             tCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGACAGGCATATTTTCAGGCg                                                                                                                      >  1:289103/1‑240 (MQ=255)
                                                                                                                                                                                 gTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACa                                                                                                                                                    >  1:106792/1‑206 (MQ=255)
                                                                                                                                                                                  tCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACttt                                                                                                                                                                                                                                                                 <  7:1527869/96‑1 (MQ=39)
                                                                                                                                                                                  tCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGtttt                                                                                                                                                                                                            <  6:472225/149‑1 (MQ=255)
                                                                                                                                                                                    tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGa                                                                                                                                                                                                                 >  8:1287707/1‑142 (MQ=255)
                                                                                                                                                                                    tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGc                                                                                                                                                                                                          >  8:1392295/1‑149 (MQ=255)
                                                                                                                                                                                         gTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACa                                                                                                                                                                                                     >  5:1330639/1‑149 (MQ=255)
                                                                                                                                                                                           aaaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACtt                                                                                                                                                                                                                                                                  <  5:1222067/86‑1 (MQ=38)
                                                                                                                                                                                               ccTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAg                                                                                                                                                                                                                                                                         <  4:706015/75‑1 (MQ=255)
                                                                                                                                                                                                cTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                                                                                                                                              <  7:1292686/149‑1 (MQ=255)
                                                                                                                                                                                                cTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                                                                                                                                              <  6:801626/149‑1 (MQ=255)
                                                                                                                                                                                                  ggCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTgg                                                                                                                                                                                            <  8:185938/149‑1 (MQ=255)
                                                                                                                                                                                                    cTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCa                                                                                                                                                                                          <  7:1115601/149‑1 (MQ=255)
                                                                                                                                                                                                       ttCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGc                                                                                                                                                                                                   <  4:734714/137‑1 (MQ=255)
                                                                                                                                                                                                           gTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACt                                                                                                                                                                                                                                                     <  2:16324/83‑1 (MQ=255)
                                                                                                                                                                                                           gTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGcc                                                                                                                                                                                   >  6:1163879/1‑149 (MQ=255)
                                                                                                                                                                                                              cTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCtt                                                                                                                                                                                >  5:1404253/1‑149 (MQ=255)
                                                                                                                                                                                                              cTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCtt                                                                                                                                                                                >  7:345651/1‑149 (MQ=255)
                                                                                                                                                                                                               tAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                                                                                                                                               <  8:475916/149‑1 (MQ=255)
                                                                                                                                                                                                                aaCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTc                                                                                                                                                                              <  7:1387802/149‑1 (MQ=255)
                                                                                                                                                                                                                aaCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATtgtg                                                <  2:326476/275‑1 (MQ=255)
                                                                                                                                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGc                                                                                                                                                                                                   <  3:151640/127‑1 (MQ=255)
                                                                                                                                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGc                                                                                                                                                                                                   >  1:151640/1‑127 (MQ=255)
                                                                                                                                                                                                                  cTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAc                                                                                                                                                                                                                        >  8:1674098/1‑105 (MQ=255)
                                                                                                                                                                                                                   tttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGt                                                                                                                                                                                                                                                                       <  8:592128/57‑1 (MQ=255)
                                                                                                                                                                                                                   tttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                                                                                                                                                 <  5:1622548/63‑1 (MQ=255)
                                                                                                                                                                                                                         tACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATa                                                                                                                                                                     >  5:142021/1‑149 (MQ=255)
                                                                                                                                                                                                                          aCGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                                                                                                                                                    <  2:722882/149‑1 (MQ=255)
                                                                                                                                                                                                                            tcgTTCGGATAAAGCGTTCGCCTGGATAAAGGCACCTGCATAGCAGGTGACTTTAACTCCCCCACGTCACCGAAAGTAGTTTCCTCATTGACTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCaaa                                <  1:72933/278‑1 (MQ=255)
                                                                                                                                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                                                                                                                                                  >  8:1235698/1‑149 (MQ=255)
                                                                                                                                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                                                                                                                                                  >  6:458972/1‑149 (MQ=255)
                                                                                                                                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                                                                                                                                                  <  7:1437666/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                                                                                                                                         >  5:504809/1‑149 (MQ=255)
                                                                                                                                                                                                                                     tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                                                                                                                                         >  6:61165/1‑149 (MQ=255)
                                                                                                                                                                                                                                      aaCTCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcaca                                                                                                                                                        <  6:1249968/149‑1 (MQ=255)
                                                                                                                                                                                                                                         gcgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGAc                                                                                                                                                     >  7:767491/1‑149 (MQ=255)
                                                                                                                                                                                                                                                ccTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTg                                                                                                                                              >  7:1624374/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGa                                                                                                                                             >  7:673831/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   ggATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAg                                                                                                                                           <  6:1492188/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      tAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                                                                                                                                               <  5:841569/110‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGaaa                                                                                                                                      <  5:61165/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         aGTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                                                                                                                                              <  8:115126/92‑1 (MQ=39)
                                                                                                                                                                                                                                                         aGTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAg                                                                                                                                     >  7:1150116/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCGAAGAGTTTACTTTTCTGCa                                                                                 >  2:33168/1‑201 (MQ=255)
                                                                                                                                                                                                                                                          gTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGtt                                                                                                                                                                                                              <  8:862285/75‑1 (MQ=38)
                                                                                                                                                                                                                                                           tCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGc                                                                                                                                   >  5:150993/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           tCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGc                                                                                                                                   <  8:32088/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCGAAGAGTTTACTTTTCTGCATTTc                                                                             <  4:33168/201‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aTAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTc                                                                                                                           <  7:529220/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                              
CTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTT  >  NC_000913/2462418‑2462946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: