Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,462,678 T→A 5.2% intergenic (+32/‑334) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,6780TA 5.2% 974.3 / 30.3 270intergenic (+32/‑334)fadL/yfdFlong‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (121/135);  new base A (7/7);  total (128/142)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-02

Only 100 of 273 total aligned reads displayed.

CTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTT  >  NC_000913/2462418‑2462946
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                              
                      gCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGAAGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                                                                                                                                             >  2:217273/1‑273 (MQ=255)
                                 ttCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCGGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGACGGCCCATACCCGTTCGAGTCTGGAGGTAAAGCCTGGATGTTCGGTACTAACTTTAACTCCGCGTTCTGAAAACGCGTTCGCCTGGACAACGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCCCACGTAGAGTACTCAt                                                                                                                                                                                                                               >  4:355048/1‑275 (MQ=255)
                                 ttCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCAt                                                                                                                                                                                                                               >  2:557093/1‑275 (MQ=255)
                                            ttCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGCCTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCCCGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGCAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCTTTCTTATAACGCGTTCGCCTTGATAACGTCACCTGCCTAGCCGGTGACTTTAACTCCCCCACTTCACCGACAGTAGTTTCCTAATAGCTTACCTCCTGac                                                                                                                                                                                                                >  1:200914/1‑278 (MQ=255)
                                             tacgaccaaGGAACGTTTCTGGCTGAGTGCAGGTACGGATTACGCATTTAATAAAAATGCTTCAGTGGACGTTGGTGTTTCTTGTATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcctcct                                                                                                                                                                                                                   <  4:169794/267‑1 (MQ=255)
                                                            tttCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTCACTTTAACTACGCGTTCTGATAACGCGTTCCCCTTGATAAAGTAACCTGCCTAGCAGGTCACTTTAAATCCCACACTTCACCGAAAGTAGTGCACCTCTGTGCTCACCCTTGAGTTTTGCAAACAg                                                                                                                                                                                                    >  4:626930/1‑275 (MQ=255)
                                                                                    ttACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCa                                                                                                                                                                             <  2:355052/274‑1 (MQ=255)
                                                                                                        cTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTACAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcaca                                                                                                                                                        >  4:48891/1‑275 (MQ=255)
                                                                                                            aGTCGACGTTGGTGTTTCTTATATGCACGGTAAGGGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACa                                                                                                                                                    <  2:626934/275‑1 (MQ=255)
                                                                                                                 aCGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtagt                                                                                                                                                                                                                                        >  3:162745/1‑187 (MQ=255)
                                                                                                                    ttGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCa                                                                                                                                                                                                                                                                           <  5:1592101/149‑1 (MQ=255)
                                                                                                                    ttGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCCGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCa                                                                                                                                                                                                                                                                           <  6:175216/149‑1 (MQ=255)
                                                                                                                        tgtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTg                                                                                                                                                                                                                                                                       >  5:900601/1‑149 (MQ=255)
                                                                                                                         gtTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                                                                                                                                                      <  8:1430337/149‑1 (MQ=255)
                                                                                                                          tttCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGAc                                                                                                                                                                                                                                                                     >  6:408660/1‑149 (MQ=255)
                                                                                                                            tCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACtt                                                                                                                                                                                                                                                                   <  7:988985/149‑1 (MQ=255)
                                                                                                                               tataTGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTaa                                                                                                                                                                                                                                                                <  5:1186239/149‑1 (MQ=255)
                                                                                                                                 tatGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGt                                                                                                                                                                                                               >  2:505093/1‑195 (MQ=255)
                                                                                                                                    gCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTccc                                                                                                                                                                                                                                                           >  8:652144/1‑149 (MQ=255)
                                                                                                                                      aCGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTc                                                                                                                                                                                                                                 <  2:261821/172‑1 (MQ=255)
                                                                                                                                       cGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAACCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTTCCCCa                                                                                                                                                                                                                                                        <  5:491455/149‑1 (MQ=255)
                                                                                                                                          tCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATa                                                                                                                                                                                                                                                                               <  7:922340/124‑1 (MQ=255)
                                                                                                                                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGa                                                                                                                                                                                                                                                                      >  6:260055/1‑129 (MQ=255)
                                                                                                                                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGa                                                                                                                                                                                                                                                                      <  5:260055/129‑1 (MQ=255)
                                                                                                                                             gagCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                                                                                                                                                  <  6:739507/149‑1 (MQ=255)
                                                                                                                                                    aaaaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCg                                                                                                             >  2:305919/1‑275 (MQ=255)
                                                                                                                                                     aaaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagta                                                                                                                                                                                                                                          >  7:1587660/1‑149 (MQ=255)
                                                                                                                                                      aaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGa                                                                                                                                                                                                                                               <  8:303232/143‑1 (MQ=255)
                                                                                                                                                      aaTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGa                                                                                                                                                                                                                                               >  7:303232/1‑143 (MQ=255)
                                                                                                                                                       aTTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagtagt                                                                                                                                                                                                                                        <  5:312559/149‑1 (MQ=255)
                                                                                                                                                         tAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGAATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAgtgt                                                                                                                                                                                                                                     >  6:1475811/1‑149 (MQ=255)
                                                                                                                                                           aCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTcc                                                                                                                                                                                                                                   <  5:1699712/149‑1 (MQ=255)
                                                                                                                                                           aCGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTcc                                                                                                                                                                                                                                   >  7:475916/1‑149 (MQ=255)
                                                                                                                                                             gAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTc                                                                                                                                                                                                                                                             <  7:597158/122‑1 (MQ=255)
                                                                                                                                                              aaGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCa                                                                                                                                                                                                                                >  7:1677940/1‑149 (MQ=255)
                                                                                                                                                                 gCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTg                                                                                                                                                                                                                             >  5:1492188/1‑149 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATTGCAGGTGa                                                                                                                                                                                                                                                                      <  7:1557064/107‑1 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGa                                                                                                                                                                                                                                                                      >  8:1557064/1‑107 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                                                                                                                                                  <  8:24907/111‑1 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACttt                                                                                                                                                                                                                                                                  >  7:24907/1‑111 (MQ=255)
                                                                                                                                                                   ccATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCt                                                                                                                                                                                                                           <  6:1554870/149‑1 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          >  8:1023261/1‑149 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          <  6:788164/149‑1 (MQ=255)
                                                                                                                                                                    cATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCtt                                                                                                                                                                                                                          <  7:550583/149‑1 (MQ=255)
                                                                                                                                                                      tACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAc                                                                                                                                                                                                                        <  8:812501/149‑1 (MQ=255)
                                                                                                                                                                       aCCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcc                                                                                                                                                                                                                       >  5:801626/1‑149 (MQ=255)
                                                                                                                                                                        ccAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCAc                                                                                                                                                                                                                                                  <  6:993824/122‑1 (MQ=255)
                                                                                                                                                                        ccAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                                                                                                                                             >  7:1149276/1‑127 (MQ=255)
                                                                                                                                                                        ccAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGaaa                                                                                                                                                                                                                                             <  8:1149276/127‑1 (MQ=255)
                                                                                                                                                                         cAGTTCGAGTCTGAAGGTAAAGCCTTGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCTTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTAcctc                                                                                                                                                                                                                     <  8:182676/149‑1 (MQ=255)
                                                                                                                                                                           gTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACNGAAAGTANTGTNCTCATTNCTTACCTCCTGAGTTTTGCAAAc                                                                                                                                                                                                      >  3:39859/1‑162 (MQ=255)
                                                                                                                                                                           gTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAAc                                                                                                                                                                                                      <  1:39859/162‑1 (MQ=255)
                                                                                                                                                                           gTTCGAGTCGTAAGGGAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGGTCGCCTGGATAAAAGTCACCTTCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCGTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAGCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCGCAAAGAGTTTACtttt                                                                                      <  4:305915/275‑1 (MQ=255)
                                                                                                                                                                             tCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGACAGGCATATTTTCAGGCg                                                                                                                      >  1:289103/1‑240 (MQ=255)
                                                                                                                                                                                 gTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACa                                                                                                                                                    >  1:106792/1‑206 (MQ=255)
                                                                                                                                                                                  tCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACt                                                                                                                                                                                                                                                                    <  8:1202406/94‑1 (MQ=39)
                                                                                                                                                                                    tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGa                                                                                                                                                                                                                 >  8:1287707/1‑142 (MQ=255)
                                                                                                                                                                                    tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGc                                                                                                                                                                                                          >  8:1392295/1‑149 (MQ=255)
                                                                                                                                                                                    tGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCGGa                                                                                                                                                                                                                 <  7:1287707/142‑1 (MQ=255)
                                                                                                                                                                                       aGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagta                                                                                                                                                                                                                                          >  6:1126409/1‑115 (MQ=255)
                                                                                                                                                                                       aGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCaaa                                                                                                                                                                                                       >  6:560817/1‑149 (MQ=255)
                                                                                                                                                                                          tAAAGCCTGGCGGTTCGGTACTAACTTTAAGAGAGGGGTCTGATAACGCGTTCGCCGTGTTAAAGTCACCTGCATAGCAGGGGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATa                                                                      <  4:58233/275‑1 (MQ=255)
                                                                                                                                                                                           aaaGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCTATGCAGGTGACtt                                                                                                                                                                                                                                                                   >  6:1222067/1‑86 (MQ=38)
                                                                                                                                                                                                cTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                                                                                                                                              <  8:612909/149‑1 (MQ=255)
                                                                                                                                                                                                   gCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAAc                                                                                                                                                                                                                                                               >  5:1248994/1‑82 (MQ=255)
                                                                                                                                                                                                        tCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCtt                                                                                                                                                                                      <  7:1518004/149‑1 (MQ=255)
                                                                                                                                                                                                           gTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACt                                                                                                                                                                                                                                                      <  2:16324/83‑1 (MQ=255)
                                                                                                                                                                                                              cTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCtt                                                                                                                                                                                >  7:345651/1‑149 (MQ=255)
                                                                                                                                                                                                               tAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                                                                                                                                               <  8:475916/149‑1 (MQ=255)
                                                                                                                                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCAt                                                                                                                                                                                                                               >  4:206277/1‑99 (MQ=255)
                                                                                                                                                                                                                 aCTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGc                                                                                                                                                                                                   >  1:151640/1‑127 (MQ=255)
                                                                                                                                                                                                                   tttAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGt                                                                                                                                                                                                                                                                        <  8:592128/57‑1 (MQ=255)
                                                                                                                                                                                                                      aaCTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCa                                                                                                                                                                        >  4:722877/1‑149 (MQ=255)
                                                                                                                                                                                                                        cTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAAt                                                                                                                                                                      <  8:1587660/149‑1 (MQ=255)
                                                                                                                                                                                                                          aCGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                                                                                                                                                    <  7:1392295/149‑1 (MQ=255)
                                                                                                                                                                                                                          aCGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                                                                                                                                                    <  2:722882/149‑1 (MQ=255)
                                                                                                                                                                                                                          aCGCGTTATCAGGACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                                                                                                                                                    >  6:656812/1‑149 (MQ=255)
                                                                                                                                                                                                                          aCGCGTTATCAGAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATaa                                                                                                                                                                    >  5:141819/1‑149 (MQ=255)
                                                                                                                                                                                                                            gcgTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGc                                                                                                                                                                  >  6:458972/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATc                                                                                                                                                            >  5:1403127/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGATAACGCGTTCGCCCGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATc                                                                                                                                                            <  7:1437613/149‑1 (MQ=255)
                                                                                                                                                                                                                                   gATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATcc                                                                                                                                                           <  5:746944/149‑1 (MQ=255)
                                                                                                                                                                                                                                     tAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcac                                                                                                                                                         >  5:504809/1‑149 (MQ=255)
                                                                                                                                                                                                                                      aaCTCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCcaca                                                                                                                                                        <  6:1249968/149‑1 (MQ=255)
                                                                                                                                                                                                                                         gcgTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGAc                                                                                                                                                     >  7:767491/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 cTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGa                                                                                                                                             >  7:673831/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    gATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAgg                                                                                                                                          >  5:420571/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      tAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                                                                                                                                               <  5:841569/110‑1 (MQ=255)
                                                                                                                                                                                                                                                      tAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCttt                                                                                                                                                                               >  6:841569/1‑110 (MQ=255)
                                                                                                                                                                                                                                                        aaaGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGaa                                                                                                                                       <  6:900601/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAagta                                                                                                                                                                                                                                          >  3:13933/1‑49 (MQ=255)
                                                                                                                                                                                                                                                          aGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCGAAGAGTTTACTTTTCTGCa                                                                                 >  2:33168/1‑201 (MQ=255)
                                                                                                                                                                                                                                                          aGTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGtt                                                                                                                                                                                              >  7:115126/1‑92 (MQ=39)
                                                                                                                                                                                                                                                           gTCACCTGCTATGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGtt                                                                                                                                                                                                              <  8:862285/75‑1 (MQ=38)
                                                                                                                                                                                                                                                            tCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGc                                                                                                                                   >  8:1512235/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            tCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGc                                                                                                                                   <  8:32088/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGa                                                                                                                                                      <  7:851728/128‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCGAAGAGTTTACTTTTCTGCATTTc                                                                             <  4:33168/201‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATAtttt                                                                                                                            <  7:1372569/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                    aTAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTc                                                                                                                           <  7:529220/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                              
CTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTATCTCCATTCCGGACCAGGACCGTTTCTGGCTGAGTGCAGGTACGACTTACGCATTTAATAAAGATGCTTCAGTCGACGTTGGTGTTTCTTATATGCACGGTCAGAGCGTGAAAATTAACGAAGGCCCATACCAGTTCGAGTCTGAAGGTAAAGCCTGGCTGTTCGGTACTAACTTTAACTACGCGTTCTGATAACGCGTTCGCCTGGATAAAGTCACCTGCATAGCAGGTGACTTTAACTCCCCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTT  >  NC_000913/2462418‑2462946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: