BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,900,322	C→T	5.6%	intergenic (‑49/‑270)	ygcW ← / → yqcE	putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,900,322	0	C	T	5.6%	700.5 / 21.4	214	intergenic (‑49/‑270)	ygcW/yqcE	putative SDR family oxidoreductase/putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (91/110); new base T (6/6); total (98/116)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.21e-01

Only 100 of 220 total aligned reads displayed.

GATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGA  >  NC_000913/2900049‑2900584

gATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTTCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCGGGGAGAAAAACTCATTTGAGAACGCATTGAGATATTCCATTGCCATAGTTAGCTCCAATTCCCGTAAGCCCAAACAAACGAAAGGTAACTTccccc                                                                                                                                                                                                                                                                        >  4:113663/1‑275 (MQ=255)

      cACCTGAATGAAGTCCACCTCAACAACCTGGTTTTCAATCATTTCCTTTGTTTCTGCGTTATCTTTGACGAAAATAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCAATGGCAAATGCCTGGCCTAAACCGCCATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCgaga                                                                                                                                                                                                                                                                  <  4:200257/275‑1 (MQ=255)

         cTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAANNNAAAAAAAGACAACTTCCAACACAAGA‑GGGactt                                                                                                                                                                                                                                                          >  3:273361/1‑276 (MQ=255)

              tGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGt                                                                                                                                                                                                                                                          <  2:27529/274‑1 (MQ=255)

                   tCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCtt                                                                                                                                                                                                                                                    >  2:390394/1‑275 (MQ=255)

                      aCCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtct                                                                                                                                                                                                                                                 >  2:35712/1‑275 (MQ=255)

                           aaCACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTa                                                                                                                                                                                                                                            >  2:417756/1‑275 (MQ=255)

                                  tGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCTTGGCCTAAACCGCTATTCCCACCGGTAAAAATTGCGGTTTTACCTTTCAGGGTGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTATCTCCCATTTCCGATAAGCAAAAAAAACGAAATGTAACTTCCCCCGCTACG‑GGTAACATACCTTTCTCATTaaccttgg                                                                                                                                                                                                                                     >  4:69520/1‑268 (MQ=255)

                                    tttttCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTg                                                                                                                                                                                                                               >  1:219866/1‑279 (MQ=255)

                                            tCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGTAATAAAGATATTTGTCCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTCAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCCGGGAGCAAAAATCCATTGAGAACGTATTGAGGGTTTCGATTGACATAGTTACCTCCATTTTCCTTAATGCAAAAATAAAGCAAGGTAACTTCCCCTG‑GATATGGGAAGTTACCTTTACCATTTTCCCTGGAAACTGgagt                                                                                                                                                                                                                           >  4:325177/1‑274 (MQ=255)

                                                 tCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTTCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTACAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTAGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTACCTCAGATAACGGGAGATAAtt                                                                                                                                                                                                                      >  4:486670/1‑275 (MQ=255)

                                                                 ttccTTTGACGAAACAAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                                                                                                                                      <  4:555778/272‑1 (MQ=255)

                                                                             aaCTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACCTAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAATTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAAATAATTTTTAGCATAAGGGCATAACTGCTAAtgt                                                                                                                                                                                          >  2:241118/1‑275 (MQ=255)

                                                                                      taaaataatTTCCGCC‑AGCTTTGGGCAACCCCATTGCAACATCCTGGCCTAAACCCGAATTCCCACCGGTAAAAATTGCGGTTTTACCTTTCAGCGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGGCATAGTTAGCTCCAATTTCCGTAAGGCCAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                                                                                                                                 <  4:35712/268‑1 (MQ=255)

                                                                                        aaaaaaattGCGCCAAGCTTTGGCC‑ACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATca                                                                                                                                                                               <  2:489046/268‑1 (MQ=255)

                                                                                              tttGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt                                                                                                                                                                         >  4:385160/1‑275 (MQ=255)

                                                                                              tttGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtg                                                                                                                                                                          >  2:44817/1‑274 (MQ=255)

                                                                                                                                  cTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCcgcg                                                                                                                                                                                                                                                                     >  7:875892/1‑149 (MQ=255)

                                                                                                                                      ccTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGaga‑g                                                                                                                                                                                                                                                                <  5:766007/149‑1 (MQ=255)

                                                                                                                                      aatAAACTGCTATTCCAATCGGTAAAAAAGTGGNTTTTAACTTTCAAGAAGAAAAAATCNATTTAGAATGCATTGAGATGTGAGAATGAAATAGTTAGATCCAATTTCTGGAAGGAAAAAAAAAAGAAAGGTAACTGCCCNCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAt                                                                                                                              <  3:155479/277‑1 (MQ=255)

                                                                                                                                        tAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑ggg                                                                                                                                                                                                                                                              >  5:225377/1‑149 (MQ=255)

                                                                                                                                          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGaa                                                                                                                                                                                                                                                            <  8:494013/149‑1 (MQ=255)

                                                                                                                                          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGaa                                                                                                                                                                                                                                                            <  8:923282/149‑1 (MQ=255)

                                                                                                                                          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATAtt                                                                                                                                                                   >  2:195227/1‑238 (MQ=255)

                                                                                                                                          aaCCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTAct                                                                                                                          >  1:273431/1‑279 (MQ=255)

                                                                                                                                               cTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTAc                                                                                                                                                                                                                                                       >  6:90699/1‑149 (MQ=255)

                                                                                                                                                     ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAGAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtct                                                                                                                                                                                                                                                 >  8:1372218/1‑149 (MQ=255)

                                                                                                                                                     ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtct                                                                                                                                                                                                                                                 >  7:1146796/1‑149 (MQ=255)

                                                                                                                                                     ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtct                                                                                                                                                                                                                                                 >  6:269061/1‑149 (MQ=255)

                                                                                                                                                     ccACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtct                                                                                                                                                                                                                                                 <  8:289264/149‑1 (MQ=255)

                                                                                                                                                      cACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTtctc                                                                                                                                                                                                                                                >  7:1198345/1‑149 (MQ=255)

                                                                                                                                                             aaCAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTAtct                                                                                                                                                                                                                                         <  7:607512/149‑1 (MQ=255)

                                                                                                                                                              aCAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTAtctc                                                                                                                                                                                                                                        <  8:418588/149‑1 (MQ=255)

                                                                                                                                                                aaTTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTg                                                                                                                                                                                                                                      >  7:1439845/1‑149 (MQ=255)

                                                                                                                                                                 aTTGCGGATTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGaa                                                                                                                                                                                                                                                            >  8:382214/1‑126 (MQ=255)

                                                                                                                                                                          ttACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATGGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                                                                                                                                             <  3:233559/196‑1 (MQ=255)

                                                                                                                                                                               tttCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAt                                                                                                                                                                                                                       >  7:903193/1‑149 (MQ=255)

                                                                                                                                                                                 tCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttt                                                                                                                                                                                                                     <  8:1198345/149‑1 (MQ=255)

                                                                                                                                                                                  cAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAtttt                                                                                                                                                                                                                    <  6:1519044/149‑1 (MQ=255)

                                                                                                                                                                                   aGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAAttttt                                                                                                                                                                                                                   <  8:17766/149‑1 (MQ=255)

                                                                                                                                                                                    gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                                                                                                                                  <  5:827425/149‑1 (MQ=255)

                                                                                                                                                                                    gggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                                                                                                                                  <  5:909424/149‑1 (MQ=255)

                                                                                                                                                                                     ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCt                                                                                                                                                                                                                                                     <  6:366574/113‑1 (MQ=255)

                                                                                                                                                                                     ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGt                                                                                                                                                                                                                                    >  5:1193914/1‑130 (MQ=255)

                                                                                                                                                                                     ggAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAg                                                                                                                                                                                                                 <  6:688628/149‑1 (MQ=255)

                                                                                                                                                                                     ggAGAAAAAATCCATTGAGAAAGCATTGAGAGATTCGATTGACATAGTTAGCTCCAAGTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTattatt                                                                                    <  2:113664/274‑1 (MQ=255)

                                                                                                                                                                                         aaaaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATa                                                                                                                                                                                                             >  7:699018/1‑149 (MQ=255)

                                                                                                                                                                                           aaaaTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTAc                                                                                                                                                                                                                                                       <  4:90388/105‑1 (MQ=255)

                                                                                                                                                                                              aTCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGc                                                                                                                                                                                                        >  6:182516/1‑149 (MQ=255)

                                                                                                                                                                                                  aTTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                                                                                                                                    >  7:1595703/1‑149 (MQ=255)

                                                                                                                                                                                                  aTTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATaa                                                                                                                                                                                                    <  8:1439845/149‑1 (MQ=255)

                                                                                                                                                                                                   ttGAGAACGCATTGAAAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAc                                                                                                                                                                                                   <  8:15776/149‑1 (MQ=255)

                                                                                                                                                                                                      agaACGCATTGAGAGATTCGATTGGCATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGc                                                                                                                                                                                                >  7:1659103/1‑149 (MQ=255)

                                                                                                                                                                                                          cGCATTGAGAGATTCGTCTGAGAAAGTTATGTCCAATTTCCGAAAGGCAAAAAAAAAGAAAGGTATCTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTATCTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAt                                                                                                                                                                                            <  8:601805/149‑1 (MQ=255)

                                                                                                                                                                                                               tGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGAt                                                                                                                                                                                       <  7:316091/149‑1 (MQ=255)

                                                                                                                                                                                                                gagagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATc                                                                                                                                                                                      <  7:1060887/149‑1 (MQ=255)

                                                                                                                                                                                                                 agagaTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                                                                                                                                             <  4:440712/157‑1 (MQ=255)

                                                                                                                                                                                                                  gagaTTCGATTGACATATTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACt                                                                                                                                                                                                                                <  7:1418172/105‑1 (MQ=255)

                                                                                                                                                                                                                     aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCAt                                                                                                                                                                                                              <  1:246338/120‑1 (MQ=255)

                                                                                                                                                                                                                     aTTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAAt                                                                                                                                                                                 <  5:1502756/149‑1 (MQ=255)

                                                                                                                                                                                                                         gATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAg                                                           <  2:208122/263‑1 (MQ=255)

                                                                                                                                                                                                                         gATTGACATAGTTAGCTCCAATTTCCGCAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcaca                                                                                                                                                                             <  5:1389830/149‑1 (MQ=255)

                                                                                                                                                                                                                           ttGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgt                                                                                                                                                                           >  8:749159/1‑149 (MQ=255)

                                                                                                                                                                                                                            tGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAgg                                                                                                                                                                                                          >  2:571731/1‑117 (MQ=255)

                                                                                                                                                                                                                             gACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCCCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAgtgt                                                                                                                                                                         <  7:39876/149‑1 (MQ=255)

                                                                                                                                                                                                                              aCATAGTTAGCCCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGtgtg                                                                                                                                                                        >  6:1622436/1‑149 (MQ=255)

                                                                                                                                                                                                                              aCATACTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCAt                                                                                                                                                                                                      <  7:544710/119‑1 (MQ=255)

                                                                                                                                                                                                                               cATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGa                                                                                                                                                                       >  8:1004508/1‑149 (MQ=255)

                                                                                                                                                                                                                                      aGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGt                                                                                                                                                                <  6:578920/149‑1 (MQ=255)

                                                                                                                                                                                                                                       gCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTa                                                                                                                                                               <  8:1311310/149‑1 (MQ=255)

                                                                                                                                                                                                                                         tCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                                                                                                                                             >  8:538211/1‑149 (MQ=255)

                                                                                                                                                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAt                                                                                                                                                                                            >  5:1090801/1‑116 (MQ=255)

                                                                                                                                                                                                                                           cAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGt                                                                                                                                                           <  7:228661/149‑1 (MQ=255)

                                                                                                                                                                                                                                             aTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTa                                                                                                                                                                                                                  >  4:224426/1‑92 (MQ=255)

                                                                                                                                                                                                                                             aTTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATcac                                                                                                                                                                              >  4:613653/1‑128 (MQ=255)

                                                                                                                                                                                                                                               ttCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCaaa                                                                                                                                                       <  6:432860/149‑1 (MQ=255)

                                                                                                                                                                                                                                                tCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAaat                                                                                                                                                      >  7:1643388/1‑149 (MQ=255)

                                                                                                                                                                                                                                                   gTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAAATGCTAATTTGATCGTAATCACCGTGTGATATTTGTATTGTCAaattat                                                                                                                                                   >  5:1296189/1‑149 (MQ=255)

                                                                                                                                                                                                                                                       ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                                                                                                                                             >  6:530409/1‑135 (MQ=255)

                                                                                                                                                                                                                                                       ggCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTAtt                                                                                                                                                             <  5:530409/135‑1 (MQ=255)

                                                                                                                                                                                                                                                          aaaaaaaaaGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTaaaa                                                                                                                                            >  8:802949/1‑149 (MQ=255)

                                                                                                                                                                                                                                                            aaaaaaaGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAg                                                                                                                                          <  5:338309/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              aaaaaGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                                                                                                                                        <  7:675491/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              aaaaaGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCa                                                                                                                                        <  6:809118/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                  agaaagGTAACTTCCCTCTCGCGG‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAg                                                                                                                                    >  7:487959/1‑149 (MQ=39)

                                                                                                                                                                                                                                                                  agaaagGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAg                                                                                                                                    >  7:733441/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                  agaaagGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcac                                                                                                                     >  2:751191/1‑164 (MQ=255)

                                                                                                                                                                                                                                                                    ataagTAACTTCCCCCGCGAGA‑TGTAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAAGGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGa  <  1:168423/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                      agGTAACTTCCCTCTCGCGG‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAAtt                                                                                                                                                  <  6:1456896/131‑1 (MQ=255)

                                                                                                                                                                                                                                                                      agGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTg                                                                                                                                <  6:111897/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                         tAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGAtt                                                                                                                             >  5:1698239/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                           aCTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTAc                                                                                                                           >  5:27055/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                            cTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGGTATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTAct                                                                                                                          <  5:386866/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                             ttCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGc                                                                                                                                   <  5:803725/139‑1 (MQ=255)

                                                                                                                                                                                                                                                                             ttCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc                                                                                                                         <  6:241589/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                             ttCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActc                                                                                                                         >  5:374146/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                             ttCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTT‑AAAAGCAGAAGCGTGATTActct                                                                                                                        >  5:195968/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                              tCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActct                                                                                                                        <  6:1053951/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                              tCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTActct                                                                                                                        <  5:1599945/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 cccGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTcac                                                                                                                     >  6:602555/1‑149 (MQ=255)

GATACCCACCTGCATGAAGTCCACCTCAACACCCTGTTTTTCAATCATTTCCTTTGTTTCGCCGTTATCTTTGACGAAACTAGGAATAAAGATATTTGCGCC‑AGCTTTGGCCAACGCCATGGCAAATGCCTGGCCTAAACCGCTATTCCCACCGGTAACAATTGCGGTTTTACCTTTCAGGGAGAAAAAATCCATTGAGAACGCATTGAGAGATTCGATTGACATAGTTAGCTCCAATTTCCGTAAGGCAAAAAAAAAGAAAGGTAACTTCCCCCGCGAGA‑GGGAAGTTACCTTTCTCATTATCTCTGGTAACTGGAGATAATTTTTAGCATAAGGGCATAACTGCTAATGTGATCGTAATCACAGTGTGATATTCGTATTGTCAAATAATTTTAAAAAGCAGAAGCGTGATTACTCTCACACAACACCTCTGCTTTCCATGTTATTTATTATTTCAATTACTAGAGACCTGAGAAAAGTAATCCACCTGAAAGGGCGGCTTACTTTTCTCTTTTTTTTGTCTATTACAAATGGGA  >  NC_000913/2900049‑2900584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`