BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,972,652	A→T	5.5%	intergenic (+15/+17)	tas → / ← lplT	putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,972,652	0	A	T	5.5%	862.7 / 25.8	236	intergenic (+15/+17)	tas/lplT	putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
Reads supporting (aligned to +/- strand): ref base A (114/109); new base T (6/7); total (120/116)
Fisher's exact test for biased strand distribution p-value = 7.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.32e-01

Only 100 of 239 total aligned reads displayed.

CCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAAC  >  NC_000913/2972384‑2972930

cccGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCg                                                                                                                                                                                                                                                                                  <  2:106466/275‑1 (MQ=255)

               aGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACGCTGCTGGGCGCAACCACGACGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATATTTATCCGGACCCATAAGAATAANACCGGNNAAAAAACGCGGTAAAAATTAAtagat                                                                                                                                                                                                                                                               >  3:17085/1‑275 (MQ=255)

                  aaaCGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgcg                                                                                                                                                                                                                                                            <  1:123299/279‑1 (MQ=255)

                                       cgTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgcg                                                                                                                                                                                                                                           <  4:232407/275‑1 (MQ=255)

                                                 tatCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGTTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTg                                                                                                                                                                                                                                  >  4:647516/1‑274 (MQ=255)

                                                        aGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGGTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                                                                                                                                                           <  2:554034/274‑1 (MQ=255)

                                                                      ggACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaa                                                                                                                                                                                                                                                                                       >  2:404657/1‑200 (MQ=255)

                                                                       gACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGaa                                                                                                                                                                                                            >  2:526284/1‑274 (MQ=255)

                                                                       gACCCTGCTCAGATGGAGCTCGCGTTTGTACGCCGTCAACAGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCGGATCAAAAGCGCCGTTATTGCCAGCGCAAACAGAGCACCGAAgccaa                                                                                                                                                                                                       >  1:212017/1‑279 (MQ=255)

                                                                             gCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCCTAAGATTAACGCCGGTTAAATCCGGTGTTAAATATTACTGACGCCGCTGCCAGATCCCCAGCCCCATTATTCCCAGACCATACACCCCCCCGAAcccaaa                                                                                                                                                                                                      >  4:345017/1‑273 (MQ=255)

                                                                                                        cGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgcg                                                                                                                                                                                                                                                            <  1:73440/193‑1 (MQ=255)

                                                                                                        cGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgcg                                                                                                                                                                                                                                                            >  3:73440/1‑193 (MQ=255)

                                                                                                                gTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCCCCAg                                                                                                                                                               >  1:280471/1‑278 (MQ=255)

                                                                                                                                  gctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGTTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGt                                                                                                                                                                                                                                                                              <  6:1505067/149‑1 (MQ=255)

                                                                                                                                     gggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaa                                                                                                                                                                                                                                                                           >  6:906526/1‑149 (MQ=255)

                                                                                                                                           aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaa                                                                                                                                                                                                                         >  2:456052/1‑193 (MQ=255)

                                                                                                                                             ccACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCgg                                                                                                                                                                                                                                                                                 >  6:439283/1‑135 (MQ=255)

                                                                                                                                             ccACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCgg                                                                                                                                                                                                                                                                                 <  5:439283/135‑1 (MQ=255)

                                                                                                                                               aCGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGccc                                                                                                                                                  <  2:664244/260‑1 (MQ=255)

                                                                                                                                                 gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACg                                                                                                                                                                                                                                                               >  8:35140/1‑149 (MQ=255)

                                                                                                                                                   tGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgc                                                                                                                                                                                                                                                             <  6:774581/149‑1 (MQ=255)

                                                                                                                                                     gATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgcgc                                                                                                                                                                                                                                                           <  8:1340207/149‑1 (MQ=255)

                                                                                                                                                         aGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGcc                                                                                                                                                                                                                                                       >  6:1719495/1‑149 (MQ=255)

                                                                                                                                                            tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAat                                                                                                                                                                                                                                                                         >  7:302460/1‑128 (MQ=255)

                                                                                                                                                            tGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAat                                                                                                                                                                                                                                                                         <  8:302460/128‑1 (MQ=255)

                                                                                                                                                             gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAt                                                                                                                                                                                                                                                   >  6:343522/1‑149 (MQ=255)

                                                                                                                                                               aaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCgg                                                                                                                                                                                    >  2:318045/1‑210 (MQ=255)

                                                                                                                                                               aaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACATATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATcc                                                                                                                                                                                                                                                 <  6:1020781/149‑1 (MQ=255)

                                                                                                                                                                   tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                                                                                                                                                              <  7:719407/116‑1 (MQ=255)

                                                                                                                                                                   tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATAtt                                                                                                                                                                                                                                                                      >  6:694137/1‑124 (MQ=255)

                                                                                                                                                                   tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                                                                                                                                             <  7:760963/149‑1 (MQ=255)

                                                                                                                                                                   tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                                                                                                                                                             <  6:1217717/149‑1 (MQ=255)

                                                                                                                                                                     aCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaa                                                                                                                                                                                                 >  2:162584/1‑191 (MQ=255)

                                                                                                                                                                       aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                                                                                                                                                         >  8:629381/1‑149 (MQ=255)

                                                                                                                                                                       aTCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                                                                                                                                                         >  5:929233/1‑149 (MQ=255)

                                                                                                                                                                        tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGc                                                                                                                                                                                                                                                                                <  6:605974/109‑1 (MQ=255)

                                                                                                                                                                        tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGc                                                                                                                                                                                                                                                                                >  5:605974/1‑109 (MQ=255)

                                                                                                                                                                         cGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTa                                                                                                                                                                                                                                                                     <  7:1681225/119‑1 (MQ=255)

                                                                                                                                                                         cGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGgc                                                                                                                                                                                                                                                             <  2:483380/127‑1 (MQ=255)

                                                                                                                                                                          gAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGtt                                                                                                                                                                                                                                      >  7:797403/1‑149 (MQ=255)

                                                                                                                                                                               tttGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGcc                                                                                                                                                                 >  4:25071/1‑213 (MQ=255)

                                                                                                                                                                                 tGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCa                                                                                                                                                                                                                               <  7:14812/149‑1 (MQ=255)

                                                                                                                                                                                  gCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAg                                                                                                                                                                                                                              <  6:1569064/149‑1 (MQ=255)

                                                                                                                                                                                   cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgc                                                                                                                                                                                                                             >  7:422306/1‑149 (MQ=255)

                                                                                                                                                                                   cATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCg                                                                                                                                                             <  2:25071/213‑1 (MQ=255)

                                                                                                                                                                                     tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                                                                                                                                                           <  7:1176972/149‑1 (MQ=255)

                                                                                                                                                                                     tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                                                                                                                                                           >  7:1351647/1‑149 (MQ=255)

                                                                                                                                                                                     tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                                                                                                                                                           >  5:845409/1‑149 (MQ=255)

                                                                                                                                                                                        ggAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaaa                                                                                                                                                                                                                        <  5:123676/149‑1 (MQ=255)

                                                                                                                                                                                          aGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACa                                                                                                                                                                                                                      <  6:117615/149‑1 (MQ=255)

                                                                                                                                                                                           gTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAg                                                                                                                                                                                                                     <  5:387611/149‑1 (MQ=255)

                                                                                                                                                                                                    aGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCgg                                                                                                                                                                                                                                                                                 >  4:719023/1‑80 (MQ=255)

                                                                                                                                                                                                    aGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGaa                                                                                                                                                                                                            <  5:1573323/149‑1 (MQ=255)

                                                                                                                                                                                                      aCGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgc                                                                                                                                                                                                          <  5:190046/149‑1 (MQ=255)

                                                                                                                                                                                                        gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGtt                                                                                                                                                                                                                                                                             <  2:719028/80‑1 (MQ=255)

                                                                                                                                                                                                        gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaaa                                                                                                                                                                                                                                                                          <  5:320648/83‑1 (MQ=255)

                                                                                                                                                                                                        gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaaa                                                                                                                                                                                                                                                                          >  6:320648/1‑83 (MQ=255)

                                                                                                                                                                                                        gTATTAGCTGAAATTGAAGCAGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCg                                                                                                                                                             <  2:204268/192‑1 (MQ=255)

                                                                                                                                                                                                         tATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaa                                                                                                                                                                                                       >  8:741576/1‑149 (MQ=255)

                                                                                                                                                                                                         tATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaa                                                                                                                                                                                                       >  8:1513521/1‑149 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      >  7:32705/1‑149 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      <  7:1595212/149‑1 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      >  7:478719/1‑149 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      <  7:647807/149‑1 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt                                                                                                                                             <  4:443819/206‑1 (MQ=255)

                                                                                                                                                                                                          aTTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATCGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      <  6:977913/149‑1 (MQ=255)

                                                                                                                                                                                                              gCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTGAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCaa                                                                                                                                                                                                                         <  6:499743/126‑1 (MQ=255)

                                                                                                                                                                                                              gCTGAAATTCGAGCGGTGCATCCGGTTTCTACGTCTCCGGCACCAAAATGTTAACGCCGGTTAAAACCGGGCTTAAAGATTAATGACGGCGCTTCCAGATCCCCCGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTAccc                                                                    <  4:294023/275‑1 (MQ=255)

                                                                                                                                                                                                               cTGAAAGTGAAGGGGTACACAATGTTTAAACTGATCCGGCACCATAAGATTAACGCCGGTTAAAGCCGGCGTTAAAAAGTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAGGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTAcccc                                                                   <  4:526280/275‑1 (MQ=255)

                                                                                                                                                                                                                tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCGTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGTTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAtccaatgccaat                                                                                                                                                                                                <  5:1399890/149‑1 (MQ=255)

                                                                                                                                                                                                                tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                                                                                                                                                   >  5:1280369/1‑114 (MQ=255)

                                                                                                                                                                                                                tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                                                                                                                                                <  8:1429193/149‑1 (MQ=255)

                                                                                                                                                                                                                       gAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACg                                                                                                                                                                                         >  5:880309/1‑149 (MQ=255)

                                                                                                                                                                                                                        aaGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAcc                                                                                                                                                                                                               <  1:167633/126‑1 (MQ=255)

                                                                                                                                                                                                                              tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATggg                                                                                                                                                                                             <  3:223687/138‑1 (MQ=255)

                                                                                                                                                                                                                                  tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAgc                                                                                                                                                                                                                    >  6:1409876/1‑111 (MQ=255)

                                                                                                                                                                                                                                  tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                                                                                                                                                <  8:532247/131‑1 (MQ=255)

                                                                                                                                                                                                                                  tCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCgg                                                                                                                                                                                    <  6:1592742/143‑1 (MQ=255)

                                                                                                                                                                                                                                    aGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTa                                                                                                                                                                            <  7:899485/149‑1 (MQ=255)

                                                                                                                                                                                                                                      gTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                                                                                                                                                          >  7:1384785/1‑149 (MQ=255)

                                                                                                                                                                                                                                        ttATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCAt                                                                                                                                                                        >  7:1077767/1‑149 (MQ=255)

                                                                                                                                                                                                                                              ttATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGc                                                                                                                                                                  <  7:1244055/149‑1 (MQ=255)

                                                                                                                                                                                                                                                aTCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTCGTACTGCAATCGCATTCCCAGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGCCa                                   >  2:229277/1‑274 (MQ=255)

                                                                                                                                                                                                                                                    ggCACCATAAGATTAATGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTcc                                                              <  2:374626/243‑1 (MQ=255)

                                                                                                                                                                                                                                                    ggCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTg                                                                                                                                                                                                                                  <  2:90600/79‑1 (MQ=255)

                                                                                                                                                                                                                                                    ggCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                                                                                                                                                      <  4:709403/107‑1 (MQ=255)

                                                                                                                                                                                                                                                    ggCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGa                                                                                                                                                            <  8:782473/149‑1 (MQ=255)

                                                                                                                                                                                                                                                        ccATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCa                      <  1:272040/279‑1 (MQ=255)

                                                                                                                                                                                                                                                          aTAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                                                                                                                                                      >  5:1085291/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATg                                                                                                                                                     >  5:1704806/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCTCTGTTTTCGCCAAGGTTTTGTACTGCAATCGAATTCCCCCCCCCGACGCTTTTTTTACCCCGCTCCTTTAGCAAAGCATTGAGCGGAACGACAAAAACGccccc                        >  2:282273/1‑274 (MQ=255)

                                                                                                                                                                                                                                                               tttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCg                                                                                                                                                             <  8:663369/136‑1 (MQ=255)

                                                                                                                                                                                                                                                                  aaCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAcc                                                                                                                                                                    <  5:290109/127‑1 (MQ=255)

                                                                                                                                                                                                                                                                   aCGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCACCAGCGAGTAAATGCCCAGCAt                                                                                                                                             >  5:1473732/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   aCGCCGGTTAAAGCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCa                                                                                                                    <  1:111666/174‑1 (MQ=255)

                                                                                                                                                                                                                                                                   aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa                                                                                                                                                                                  >  4:490613/1‑112 (MQ=255)

                                                                                                                                                                                                                                                                   aCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt                                                                                                                                             >  5:162956/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                    cGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATc                                                                                                                                            <  7:629381/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                       cGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaac                                                                                                                                         <  6:252329/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                         gTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa                                                                                                                                       <  6:1275363/149‑1 (MQ=255)

CCCGCTATAGCGGTGAGCAAACGCAAAAAGCCGTCGCGGCGTATGTTGATATCGCCAGACGTCATGGCCTGGACCCTGCTCAGATGGCGCTCGCGTTTGTACGCCGTCAACCGTTTGTTGCCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTGTTTTCGCCAAGGTTTTGTACTGCAATCGCATTCCCCGCCCCGACGCTTTTTTTACCCCGCTCCTGTAGCAACGCATTGAGCGGAACGACAAAAAAGCCCCCCATCACGCCAATCAGCATCAAC  >  NC_000913/2972384‑2972930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`