Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,590 C→A 9.2% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5900CA 9.2% 1062.3 / 67.8 310intergenic (+72/‑33)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base C (119/160);  new base A (18/11);  total (137/173)
Fisher's exact test for biased strand distribution p-value = 5.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 314 total aligned reads displayed.

CGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTG  >  NC_000913/3156318‑3156855
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                         
         gccgcGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCCCCGGTGa                                                                                                                                                                                                                                                                >  2:592955/1‑275 (MQ=255)
          ccgcGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCCCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAg                                                                                                                                                                                                                                                               >  4:508671/1‑275 (MQ=255)
                               aTTTCGTTGACCAATTAGGCGTGCATCCCCACCTCTCCGACTACGGTCTGTACCGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                                                                                                                                                                                      <  1:90606/279‑1 (MQ=255)
                                                    tGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                                                                                                                                                                                                     <  2:267155/275‑1 (MQ=255)
                                                        caCCCACCGCTCCGAGTGCGGTCTGGAGTGGAGGTCCATCCCGGGTTTGCGTAAAAAACTGGAAGGGCACGGGATGCCCCAACTGGGCTAAAATAATGACATTACTTTGGATGTCAGCCGCCGTTTATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                                                                                                                                                                                                  <  4:596656/273‑1 (MQ=255)
                                                               ctctCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGCGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGc                                                                                                                                                                                                       >  1:168611/1‑278 (MQ=255)
                                                               ctccCCGACTACGGTCGGGACGGCAGTGCCATCCCGGCTTTGCTAAAAAAACGGGAAGGGCAAGGCATGACCCAACGGGGCGAAAATCATGACATTACGTTGGAGGGCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                                                                                                                                           <  4:315306/274‑1 (MQ=255)
                                                                       cTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTCCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCCCCGGTGATTTCAGATAAAGGAGGAAAGTATGGCCAATCCACCCGTTATTAAGCAACAGGAGGCCAATGt                                                                                                                                                                                                  >  4:327565/1‑275 (MQ=255)
                                                                             tCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCATATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCGCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                                                                                                                                                                                            <  2:133660/275‑1 (MQ=255)
                                                                                ggACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCa                                                                                                                                                                                         >  2:234250/1‑275 (MQ=255)
                                                                                                                           cACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTCATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGCGGAAGTAATCACCg                                                                                                                                          >  1:10115/1‑279 (MQ=255)
                                                                                                                               gCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGc                                                                                                                                                                                                                                                                        <  8:399755/149‑1 (MQ=255)
                                                                                                                                      ccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                                                                                                                                                 >  8:332786/1‑149 (MQ=255)
                                                                                                                                      caaattGGGCGAAATACATGCAATTACTTTGGAGTGCGACCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTNAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGGGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaa                                                                                                                                <  3:203176/273‑1 (MQ=255)
                                                                                                                                           tGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTc                                                                                                                                                                                                                                                            <  5:1160727/149‑1 (MQ=255)
                                                                                                                                             ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCat                                                                                                                                                                                                                                                          <  5:1298537/149‑1 (MQ=255)
                                                                                                                                             ggCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGTGAggtgtt                                                                                                                                                                                                                                                          <  7:1503268/149‑6 (MQ=255)
                                                                                                                                               cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCatat                                                                                                                                                                                                                                                        >  4:272850/1‑149 (MQ=255)
                                                                                                                                                gAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                                                                                                                                       >  7:703042/1‑149 (MQ=255)
                                                                                                                                                  aaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATaaa                                                                                                                                                                                                                                                     <  6:1462106/149‑1 (MQ=255)
                                                                                                                                                         gACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                                                                                                                                                  >  7:863076/1‑145 (MQ=255)
                                                                                                                                                          aCATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAAc                                                                                                                                                                                                                                             >  5:931196/1‑149 (MQ=255)
                                                                                                                                                              tACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTAt                                                                                                                                                                                                                                         <  7:1551252/149‑1 (MQ=255)
                                                                                                                                                                cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTGCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACATATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCt                                                                                                         <  2:191177/275‑1 (MQ=255)
                                                                                                                                                                  ttGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                                                                                                                                           <  2:298668/175‑1 (MQ=255)
                                                                                                                                                                         tCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCaa                                                                                                                                                                                                                              >  6:1614010/1‑149 (MQ=255)
                                                                                                                                                                           aGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAcc                                                                                                                                                                                                                            <  8:1389252/149‑1 (MQ=255)
                                                                                                                                                                           aGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCaa                                                                                                                                                                                                     >  4:84617/1‑172 (MQ=255)
                                                                                                                                                                            gccgccGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCg                                                                                                                                                                                                                           >  5:33423/1‑149 (MQ=255)
                                                                                                                                                                            gccgccGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCCCCGGTGa                                                                                                                                                                                                                                                                >  7:185882/1‑112 (MQ=255)
                                                                                                                                                                               gccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagc                                                                                                                                                                 <  2:669779/204‑1 (MQ=255)
                                                                                                                                                                                 cgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                                                                                                                                         >  4:149530/1‑146 (MQ=255)
                                                                                                                                                                                   tatataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                                                                                                                                                    >  7:935365/1‑149 (MQ=255)
                                                                                                                                                                                       taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTa                                                                                                                                                                                                                >  7:725905/1‑149 (MQ=255)
                                                                                                                                                                                          gAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGa                                                                                                                                                                                                                                                                <  7:493334/98‑1 (MQ=255)
                                                                                                                                                                                             gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                                                                                                                                                                                                                                  >  2:758950/1‑93 (MQ=255)
                                                                                                                                                                                             gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGAt                                                                                                                                                                                                          <  5:1686703/149‑1 (MQ=255)
                                                                                                                                                                                             gccgccCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaa                                                                                                                                <  1:280234/223‑1 (MQ=255)
                                                                                                                                                                                               cgcccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                                                                                                                                           <  5:891940/146‑1 (MQ=255)
                                                                                                                                                                                                 cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGAt                                                                                                                                                                                                          >  2:318443/1‑145 (MQ=255)
                                                                                                                                                                                                 cccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGt                                                                                                                                                             >  4:246928/1‑190 (MQ=255)
                                                                                                                                                                                                  ccgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACgaag                                                                        <  2:436719/274‑1 (MQ=255)
                                                                                                                                                                                                       aaGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCa                                                                                                                                                                                                >  8:1222027/1‑149 (MQ=255)
                                                                                                                                                                                                         gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACTCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATg                                                                                                                                                                                              <  8:168223/149‑1 (MQ=255)
                                                                                                                                                                                                         gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGt                                                                                                                                                                                                                                                                  <  5:741808/81‑1 (MQ=39)
                                                                                                                                                                                                         gCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACAACTCACCGGAGCAGGCTCCGGt                                                                                                                                                                                                                                                                  >  6:741808/1‑81 (MQ=39)
                                                                                                                                                                                                          cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGc                                                                                                                                                                                             <  5:1614661/149‑1 (MQ=255)
                                                                                                                                                                                                          cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGc                                                                                                                                                                                             <  7:1222027/149‑1 (MQ=255)
                                                                                                                                                                                                          cTTTTCACCCCTCCAATCTTCCTTTTNNGGGATNTCTGTCCAGCAGTAAGTTGGCAACACCTCCCTGGAGCTTGCTCCGGGTAGTTAAGTTAAAGGAGGATCGTATGGCTAATCCAACCGGTATTAAGCTACCGGATGGGAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTACTCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCa                                                            <  3:12717/279‑1 (MQ=255)
                                                                                                                                                                                                           tttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                                                                                                                                                                                               >  6:1415081/1‑82 (MQ=255)
                                                                                                                                                                                                           tttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                                                                                                                                                                                               <  5:1415081/82‑1 (MQ=255)
                                                                                                                                                                                                           ttattACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTaa                                                                                                                                                                                                                    >  8:1499480/4‑125 (MQ=255)
                                                                                                                                                                                                               tACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCaaa                                                          <  2:55681/275‑1 (MQ=255)
                                                                                                                                                                                                                   ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGAt                                                                                                                                                                                                          >  6:111701/1‑127 (MQ=255)
                                                                                                                                                                                                                   ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                                                                                                                                                    <  5:432760/149‑1 (MQ=255)
                                                                                                                                                                                                                   ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                                                                                                                                                    <  6:129036/149‑1 (MQ=255)
                                                                                                                                                                                                                    cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                                                                                                                                                   >  8:794189/1‑149 (MQ=255)
                                                                                                                                                                                                                    cTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                                                                                                                                                   >  6:1686799/1‑149 (MQ=255)
                                                                                                                                                                                                                     taaaaCTTTCGGTTGCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                                                                                                                                                  >  6:389640/3‑149 (MQ=255)
                                                                                                                                                                                                                     tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGc                                                                                                                                                                                                                                                                        >  7:538989/1‑63 (MQ=255)
                                                                                                                                                                                                                     tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGa                                                                                                                                                                                  >  6:1573120/1‑149 (MQ=255)
                                                                                                                                                                                                                       aaaCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                                                                                                                                           >  8:1649412/1‑122 (MQ=255)
                                                                                                                                                                                                                        aaCTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTg                                                                                                                                                                               >  5:248764/1‑149 (MQ=255)
                                                                                                                                                                                                                           tttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGc                                                                                                                                                                            >  6:328556/1‑149 (MQ=255)
                                                                                                                                                                                                                            ttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCg                                                                                                                                                                           >  6:1210161/1‑149 (MQ=255)
                                                                                                                                                                                                                              cGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGt                                                                                                                                                                          >  4:381193/1‑148 (MQ=255)
                                                                                                                                                                                                                                ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTg                                                                                                                                                                       >  8:1111768/1‑149 (MQ=255)
                                                                                                                                                                                                                                ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTg                                                                                                                                                                       <  8:703042/149‑1 (MQ=255)
                                                                                                                                                                                                                                  ttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTgg                                                                                                                                                                      <  2:381197/148‑1 (MQ=255)
                                                                                                                                                                                                                                    cGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaa                                                                                                                                                                   <  5:1346614/149‑1 (MQ=255)
                                                                                                                                                                                                                                      ggCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTCTTAAGCTACAGGATGGCAATGTCGTGCCCCAGCTGGGACTGGGCGTCTGgcaagc                                                                                                                                                                 >  5:491913/1‑149 (MQ=255)
                                                                                                                                                                                                                                        cATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagcaa                                                                                                                                                               <  7:1012125/149‑1 (MQ=255)
                                                                                                                                                                                                                                            tCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTaa                                                                                                                                                           <  6:1064323/149‑1 (MQ=255)
                                                                                                                                                                                                                                             cGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAAt                                                                                                                                                          <  8:725905/149‑1 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata                                                                                                                                                                                                                                                         <  8:53106/52‑1 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                                                                                                                                        <  7:1528254/149‑1 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                                                                                                                                        <  5:1540925/149‑1 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                                                                                                                                        >  5:1227087/1‑149 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCACGCCCCATTTGGCACTGTGCCTCTGGCAAGCACGTaagaa                                                                                                                                                        <  8:466718/149‑4 (MQ=255)
                                                                                                                                                                                                                                                tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTCCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                                                                                                                                        <  7:1151762/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   aGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCATCTGGCAAGCAAGTAATGAGGa                                                                                                                                                     >  6:672931/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       ttAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAAt                                                                                                                                                                                                    <  6:820643/98‑1 (MQ=255)
                                                                                                                                                                                                                                                            ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCaa                                                                                                                                                                                                                              <  6:150345/67‑1 (MQ=255)
                                                                                                                                                                                                                                                            ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGTAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGaa                                                                                                                                                    <  7:1419081/141‑1 (MQ=255)
                                                                                                                                                                                                                                                            ttCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAc                                                                                                                                            >  7:1374490/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               acaacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGc                                                                                                                                         >  6:539088/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                caacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTTTGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAc                                                                                                                                            <  5:297521/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                caacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAc                                                                                                                                            >  6:297521/1‑145 (MQ=255)
                                                                                                                                                                                                                                                                 aacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                                                                                                                                        <  8:1530363/132‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAg                                                                                                                                                       >  6:908844/1‑128 (MQ=255)
                                                                                                                                                                                                                                                                      cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCa                                                                                                                                       <  8:56041/144‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaa                                                                                                                                <  6:413850/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaa                                                                                                                                >  8:1060087/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         aCCGGAGCAGGCTCGGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaaaa                                                                                                                               >  7:127587/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         aCCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc                                                                                                                                                                                 >  6:1353283/1‑99 (MQ=255)
                                                                                                                                                                                                                                                                           cGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGc                                                                                                                             <  7:33948/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgca                                                                                                                                                                    <  1:56721/108‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gAGCATGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgca                                                                                                                                                                    >  3:56721/1‑108 (MQ=255)
                                                                                                                                                                                                                                                                               gCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTa                                                                                                                         <  7:633186/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaagca                                                                                                                                                                >  5:524354/1‑109 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                         
CGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGAAGTGGGTTATCGCTCGATTGATACCGCCGCGGCCTACAAGAACGAAGAAGGTGTCGGCAAAGCCCTGAAAAATGCCTCAGTCAACAGAGAAGAACTGTTCATCACCACTAAGCTGTG  >  NC_000913/3156318‑3156855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: