Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,186,138 A→T 5.0% intergenic (+48/‑49) yqiG → / → insC1 pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,186,1380AT 5.0% 557.8 / 14.9 160intergenic (+48/‑49)yqiG/insC1pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA
Reads supporting (aligned to +/- strand):  ref base A (74/77);  new base T (4/4);  total (79/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-01

Only 100 of 161 total aligned reads displayed.

AGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAG  >  NC_000913/3185868‑3186356
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                          
aGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCCGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTCATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGTTGTATAAAAAATTAAAATCAACCACAATAAGCGACTTGATTAAAAATCTTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCCCTTAAATaa                                                                                                                                                                                                                         >  2:264842/1‑274 (MQ=255)
                 gttCGTATGGAAGCCCCAAACAAAGATGCCACACCGGTCTACTTTTACGGCAAGGATGACTACAAGAATGCTTAAGAGTAATAGCATGTGGCGGGGTTAGCACGGGCAAATTATAAACGGAATAATGATAAGTGGATCAGATGTATAAAAAATCAAAATTAACCAAGATACGCGAACTGATTAAAAATATCTATTGTTCATTTTCCGTTATTCGACTGGGTTCCTGAATTAGCTGACAACCAATGAAACTTCAATAAGTGATAGTGTTAATAcg                                                                                                                                                                                                        <  4:264839/272‑2 (MQ=255)
                      tATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGa                                                                                                                                                                                              <  1:27556/279‑1 (MQ=255)
                                                                             tACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAAACACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGa                                                                                                                                           <  2:149393/275‑1 (MQ=255)
                                                                                                                           cacaTCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAAt                                                                                                                                                                                                                           >  5:275696/1‑149 (MQ=255)
                                                                                                                              atcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGCCTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAg                                                                                                                                                                                                                        >  7:150134/1‑149 (MQ=255)
                                                                                                                              atcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAg                                                                                                                                                                                                                        <  5:706663/149‑1 (MQ=255)
                                                                                                                              atcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAg                                                                                                                                                                                                                        <  5:1039647/149‑1 (MQ=255)
                                                                                                                               tcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                                       <  7:1515116/149‑1 (MQ=255)
                                                                                                                               tcatTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                                       <  5:227717/149‑1 (MQ=255)
                                                                                                                                 atTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTATTTAAGTGa                                                                                                                                                                                                                     >  6:827155/1‑149 (MQ=255)
                                                                                                                                    aaCGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAg                                                                                                                                                                                                                  >  5:1701622/1‑149 (MQ=255)
                                                                                                                                     aCGGATTAATGATAAGTGGATCAGATGTATAAAGAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGt                                                                                                                                                                                                                 >  5:1069264/1‑149 (MQ=255)
                                                                                                                                     aCGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTATTTAAGTGATatt                                                                                                                                                                                                                 <  5:1658521/149‑3 (MQ=255)
                                                                                                                                     aCGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGt                                                                                                                                                                                                                 <  7:1687953/149‑1 (MQ=255)
                                                                                                                                     aCGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGt                                                                                                                                                                                                                 >  7:163970/1‑149 (MQ=255)
                                                                                                                                      cGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTATTTAAGTGATattg                                                                                                                                                                                                                <  5:827155/149‑4 (MQ=255)
                                                                                                                                          ttAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                                            <  5:1486800/149‑1 (MQ=255)
                                                                                                                                          ttAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                                            <  8:1067236/149‑1 (MQ=255)
                                                                                                                                          ttAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                                            >  8:227508/1‑149 (MQ=255)
                                                                                                                                             aTGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATAc                                                                                                                                                                                                         <  6:1371305/149‑1 (MQ=255)
                                                                                                                                             aTGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATAc                                                                                                                                                                                                         >  5:1337838/1‑149 (MQ=255)
                                                                                                                                             aTGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATAc                                                                                                                                                                                                         >  5:636876/1‑149 (MQ=255)
                                                                                                                                              tGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACt                                                                                                                                                                                                        >  8:1591441/1‑149 (MQ=255)
                                                                                                                                               gATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTATTTAAGTGATattggt                                                                                                                                                                                                              <  5:1612871/142‑6 (MQ=255)
                                                                                                                                               gATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTATTTAAGTGATattggt                                                                                                                                                                                                              >  6:1612871/1‑137 (MQ=255)
                                                                                                                                               gATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGa                                                                                                                                                                                                                     >  8:521171/1‑135 (MQ=255)
                                                                                                                                               gATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGa                                                                                                                                                                                                                     <  7:521171/135‑1 (MQ=255)
                                                                                                                                                aTAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                                                                                                                                                                                                      <  6:1342660/149‑1 (MQ=255)
                                                                                                                                                   aGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                                       >  8:219666/1‑129 (MQ=255)
                                                                                                                                                   aGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGt                                                                                                                                                                                                                       <  7:219666/129‑1 (MQ=255)
                                                                                                                                                   aGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGttt                                                                                                                                                                                                   <  8:834156/149‑1 (MQ=255)
                                                                                                                                                    gTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGtttt                                                                                                                                                                                                  >  8:344526/1‑149 (MQ=255)
                                                                                                                                                      ggATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTa                                                                                                                                                                                                <  6:966690/149‑1 (MQ=255)
                                                                                                                                                       gATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAg                                                                                                                                                                                               >  7:803365/1‑149 (MQ=255)
                                                                                                                                                        aTCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATa                                                                                                                                                                                                                   >  8:1290284/1‑128 (MQ=255)
                                                                                                                                                         tCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGAc                                                                                                                                                                                             <  5:918236/149‑1 (MQ=255)
                                                                                                                                                           aGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTa                                                                                                                                                                                           >  7:924347/1‑149 (MQ=255)
                                                                                                                                                             aTGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                                         >  8:405647/1‑149 (MQ=255)
                                                                                                                                                             aTGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                                         <  6:296988/149‑1 (MQ=255)
                                                                                                                                                               gTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACt                                                                                                                                                                                                        <  1:116808/132‑1 (MQ=255)
                                                                                                                                                               gTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACt                                                                                                                                                                                                        >  3:116808/1‑132 (MQ=255)
                                                                                                                                                               gTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCa                                                                                                                                                                                       >  8:1161282/1‑149 (MQ=255)
                                                                                                                                                                 ataAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAtt                                                                                                                                                                                     <  8:803365/149‑1 (MQ=255)
                                                                                                                                                                    aaaaaTTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGga                                                                                                                                                                                  <  8:162813/149‑1 (MQ=255)
                                                                                                                                                                    aaaaaTTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGga                                                                                                                                                                                  >  7:597373/1‑149 (MQ=255)
                                                                                                                                                                     aaaattaaaattAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGgag                                                                                                                                                                                 >  7:387043/1‑149 (MQ=255)
                                                                                                                                                                     aaaattaaaattAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGgag                                                                                                                                                                                 <  7:986231/149‑1 (MQ=255)
                                                                                                                                                                       aattaaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGaa                                                                                                                                                                               <  6:1069264/149‑1 (MQ=255)
                                                                                                                                                                        attaaaattaaTCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGATATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACATAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGaa                                                                                                                                                                               >  8:1158527/1‑148 (MQ=255)
                                                                                                                                                                        attaaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGaa                                                                                                                                                                               <  7:1158527/148‑1 (MQ=255)
                                                                                                                                                                         ttaaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACGAGTTTTTAGACTAGTCATTGGAGcaca                                                                                                                                                                             >  8:1457674/1‑149 (MQ=255)
                                                                                                                                                                           aaaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAga                                                                                                                                                                           <  6:679988/149‑1 (MQ=255)
                                                                                                                                                                            aaattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgat                                                                                                                                                                          <  7:1161282/149‑1 (MQ=255)
                                                                                                                                                                             aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                                            <  6:499485/114‑1 (MQ=255)
                                                                                                                                                                             aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTaa                                                                                                                                                                                                            >  5:499485/1‑114 (MQ=255)
                                                                                                                                                                             aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatg                                                                                                                                                                         >  8:624691/1‑149 (MQ=255)
                                                                                                                                                                             aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatg                                                                                                                                                                         >  6:1312590/1‑149 (MQ=255)
                                                                                                                                                                             aattaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatg                                                                                                                                                                         >  5:172903/1‑149 (MQ=255)
                                                                                                                                                                               ttaaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAgatgat                                                                                                                                                                       >  8:36597/1‑149 (MQ=255)
                                                                                                                                                                                taaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgatt                                                                                                                                                                      <  7:1363989/149‑1 (MQ=255)
                                                                                                                                                                                 aaCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattg                                                                                                                                                                     >  5:1466439/1‑149 (MQ=255)
                                                                                                                                                                                   ccACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattgat                                                                                                                                                                   <  7:344526/149‑1 (MQ=255)
                                                                                                                                                                                   ccACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattgat                                                                                                                                                                   <  7:1271627/149‑1 (MQ=255)
                                                                                                                                                                                    cacaATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAg                                                                                                                                                                                               <  6:1384765/120‑1 (MQ=255)
                                                                                                                                                                                     acaATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGt                                                                                                                                                                 <  6:160896/149‑1 (MQ=255)
                                                                                                                                                                                       aaTAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCt                                                                                                                                                               <  7:244576/149‑1 (MQ=255)
                                                                                                                                                                                          aaGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAg                                                                                                                                                            <  6:1540761/149‑1 (MQ=255)
                                                                                                                                                                                          aaGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGACGCTTGATGTCTTAg                                                                                                                                                            <  7:125038/149‑1 (MQ=255)
                                                                                                                                                                                           aGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAgg                                                                                                                                                           <  7:405647/149‑1 (MQ=255)
                                                                                                                                                                                            gCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAg                                                                                                                                                                                                                        <  8:388584/87‑1 (MQ=255)
                                                                                                                                                                                             cGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGc                                                                                                                                                         <  6:172903/149‑1 (MQ=255)
                                                                                                                                                                                                    aTTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTa                                                                                                                                                                                           <  6:168839/108‑1 (MQ=255)
                                                                                                                                                                                                    aTTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaa                                                                                                                                                  >  8:385616/1‑149 (MQ=255)
                                                                                                                                                                                                     ttAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaaa                                                                                                                                                 >  7:1340862/1‑149 (MQ=255)
                                                                                                                                                                                                     ttAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaaa                                                                                                                                                 >  6:31129/1‑149 (MQ=255)
                                                                                                                                                                                                     ttAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaaa                                                                                                                                                 <  8:150134/149‑1 (MQ=255)
                                                                                                                                                                                                        caaaTATTTATTGATCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATACGTCATAGTCTTAATACTAGGTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGc                                                                                                                                              >  8:1462071/2‑149 (MQ=255)
                                                                                                                                                                                                            tatttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTc                                                                                                                                                                                                                >  7:24275/1‑79 (MQ=255)
                                                                                                                                                                                                              tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACgg                                                                                                                                        >  7:1590632/1‑149 (MQ=255)
                                                                                                                                                                                                              tttattGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACgg                                                                                                                                        >  6:871850/1‑149 (MQ=255)
                                                                                                                                                                                                                  ttGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTa                                                                                                                                    <  5:1351492/149‑1 (MQ=255)
                                                                                                                                                                                                                     ttCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACca                                                                                                                                 <  8:1064544/149‑1 (MQ=40)
                                                                                                                                                                                                                      tCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcac                                                                                                                                >  7:99629/1‑149 (MQ=40)
                                                                                                                                                                                                                       cATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGgaaggaag  >  2:547421/1‑274 (MQ=34)
                                                                                                                                                                                                                        aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTg                                                                                                                                                                                    <  8:1097103/95‑1 (MQ=255)
                                                                                                                                                                                                                        aTTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcacac                                                                                                                              >  8:198933/1‑149 (MQ=38)
                                                                                                                                                                                                                         ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcacaca                                                                                                                             >  5:474185/1‑149 (MQ=38)
                                                                                                                                                                                                                         ttATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcacaca                                                                                                                             <  8:1687340/149‑1 (MQ=38)
                                                                                                                                                                                                                          tATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAg                                                                                                                            <  6:225370/149‑1 (MQ=37)
                                                                                                                                                                                                                            tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTg                                                                                                                                                                                    <  6:64487/91‑1 (MQ=38)
                                                                                                                                                                                                                            tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTg                                                                                                                                                                                    >  5:64487/1‑91 (MQ=38)
                                                                                                                                                                                                                            tCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGga                                                                                                                                                                                  <  7:1189192/93‑1 (MQ=38)
                                                                                                                                                                                                                               gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGaaaa                                                                                                                       >  5:1185017/1‑149 (MQ=32)
                                                                                                                                                                                                                               gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGaaaa                                                                                                                       <  5:211491/149‑1 (MQ=32)
                                                                                                                                                                                                                               gTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGaaaa                                                                                                                       >  7:800515/1‑149 (MQ=32)
                                                                                                                                                                                                                                ttattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAg                                                                                                                      >  5:954502/1‑149 (MQ=32)
                                                                                                                                                                                                                                 tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTGAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAGTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGa                                                                                                                     <  5:375400/149‑1 (MQ=14)
                                                                                                                                                                                                                                 tattaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGa                                                                                                                     <  5:154523/149‑1 (MQ=31)
                                                                                                                                                                                                                                  attaGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGAt                                                                                                                    <  6:1337838/149‑1 (MQ=11)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                          
AGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATACCAGGAAGGAAG  >  NC_000913/3185868‑3186356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: