Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,603 T→A 7.2% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,6030TA 7.2% 853.2 / 40.6 247intergenic (+36/+383)hdeD/arrSacid‑resistance membrane protein/antisense sRNA ArrS, function unknown
Reads supporting (aligned to +/- strand):  ref base T (105/122);  new base A (10/8);  total (116/131)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 259 total aligned reads displayed.

TTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATGAGTTGGTATCGTCAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCATGGCTGATATTTTCCGTAGTCAGGTTTAATGTTTTAAAAGTGCTGTGGGAAAGTGAACAAAGAGTTCCGTAAGCGTTGATGCTATGGGCGGTTAA  >  NC_000913/3657333‑3657856
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                             
tttttttgtggtggCAGGGGTTATGGTCCTGNNGAGTTGGGATTGTATGCTTGTAATGGAATGNAACTGGGTACCGGTCGTTTTTGTTGGGCTGGATATGGTCATGGCCGGGATATTCCGTGGTGAAACGGAGATTGTNTCTGGAACGCTGGTTTCAACACTGGTGGGAATTGAACTGATATTGAGCGCCGCCAGCCTGTTCAGCTTGGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACt                                                                                                                                                                                                                                                         <  3:107779/264‑1 (MQ=255)
         tgtTGCAGGGGTTATTCGCCTGATGAGTTGGTATCGTCAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATc                                                                                                                                                                                                                                                   >  2:671903/1‑274 (MQ=255)
              acggggttttctcccTGGTGGGTTGGTTTGGTCATGGTGCAATGAAAGGCAGAGGGTTACAGCTTGTTAATGGTGTGTTGGGTATGGTCATTGCCTGTATATTCCTTGGTGCAACGCCGTTGGTGTCTGTAACGCTGGTTTCCACACTGCTGGTAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTa                                                                                                                                                                                                                                             <  4:404413/262‑1 (MQ=255)
                      aTTCGCCTGATGAGTTGGTATCGTCAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTTTGCTGGATAACGTCATTGCCTGGATATTCCTTGGTTCATCGCCTATGGTGTCTGTAACGCCGGTTTTCACACTGGTGGGAATTGAACCGATATTTAGCGCCGCCAGCCTGTTCAGCGTCGCCAGCCTGTTCGCTAAGCATCAATCCTTCACCCGGGTCTCCCCCGGAACATCGTCCCTCGAGGTGACTATGTTCCCACTAAtccact                                                                                                                                                                                                                                      >  4:154288/1‑269 (MQ=255)
                              gatgaGTTGGTATCGTCAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                                                                                                                          <  1:157997/278‑1 (MQ=255)
                                tgaGTTGGTATCGTCGGCGGTCAATGAAAGGCAGCTGGTTACAGCTCGTGATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGAGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                                                                                                                                            <  2:191663/274‑1 (MQ=255)
                                 gaGTTGGTATCGTAAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGc                                                                                                                                                                                                                           <  2:140237/274‑1 (MQ=255)
                                     gggTATCGTCAGCGTTCAATGAAAGGCAGCTGGTTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCCGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtgt                                                                                                                                                                                                                      <  2:590024/274‑1 (MQ=255)
                                                                  cTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                                                                                                                                                                                >  2:508183/1‑252 (MQ=255)
                                                                                                   cGTCATTGCCTGGATATTCCTTGGTTCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTAATATCTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTACGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGCTCCGATTCATACTTTCCCCGCTACGCAGTGTTGTAGATCAATTGCGCTCTATCCTTGAAAACATTACCTGCTAGTGATTATTTCaccttcatg                                                                                                                                                        >  4:375039/1‑267 (MQ=255)
                                                                                                                         ggTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATATGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                                                                                                                                            >  1:252309/1‑185 (MQ=255)
                                                                                                                          gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                                                                                                                                                                                               <  6:950150/149‑1 (MQ=255)
                                                                                                                            gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgt                                                                                                                                                                                                                                                             <  6:707623/149‑1 (MQ=255)
                                                                                                                            gCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgt                                                                                                                                                                                                                                                             <  5:1008558/149‑1 (MQ=255)
                                                                                                                              aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGa                                                                                                                                                                                                                                                           <  6:619939/149‑1 (MQ=255)
                                                                                                                              aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTCGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGCGa                                                                                                                                                                                                                                                           <  7:1123159/149‑1 (MQ=255)
                                                                                                                                 gCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTa                                                                                                                                                                                                                                                        <  7:1071243/149‑1 (MQ=255)
                                                                                                                                  ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                                                                                                                                                                                       <  5:1619313/149‑1 (MQ=255)
                                                                                                                                  ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                                                                                                                                                                                       <  6:1299059/149‑1 (MQ=255)
                                                                                                                                     aTGGTGTCTGAAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATACCCCGGTTGTCACCCGGATCATAGTCACTTGATGNGACTATGATCCGATTATTACTCTTTCCCCTACGCAgtgt                                                                                                                                                                                                                      >  3:134639/1‑178 (MQ=255)
                                                                                                                                     aTGGTGTCCGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATACTTACCCCGGTTGTCACCCGGATCATAGTCACTTGATTTGACTATGATCCGATTAATACTCTCTCCGCTACGAAGTGTTGTAGCTCAATTgcgcc                                                                                                                                                                                                     >  1:235134/1‑195 (MQ=255)
                                                                                                                                       ggTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATcc                                                                                                                                                                                                                                                  <  5:1366275/149‑1 (MQ=255)
                                                                                                                                         tgtCTGTAACACTGGTTTCCAAACTTGTGGGAAGTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAATTTGTTCGTTAATCAGCAATAATTACCCCGGTTGTCCCCCGGATCATAGTTACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACACAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg                                                                                                                   <  4:64572/274‑1 (MQ=255)
                                                                                                                                             tGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTaa                                                                                                                                                                                                                                            <  7:1515718/149‑1 (MQ=255)
                                                                                                                                             tGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTaa                                                                                                                                                                                                                                            >  8:1159234/1‑149 (MQ=255)
                                                                                                                                               tAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATa                                                                                                                                                                                                                                          >  5:1524413/1‑149 (MQ=255)
                                                                                                                                                aaCGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                                                                                                                                                                                         >  6:899781/1‑149 (MQ=255)
                                                                                                                                                  cGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                                                                                                                                                                                       <  8:1313231/149‑1 (MQ=255)
                                                                                                                                                    cTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                                                                                                                                                                                     <  6:410924/149‑1 (MQ=255)
                                                                                                                                                    cTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                                                                                                                                                                                     <  8:1490755/149‑1 (MQ=255)
                                                                                                                                                     tGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctct                                                                                                                                                                                                                                    <  5:1555217/149‑1 (MQ=255)
                                                                                                                                                      ggTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGGTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCGGATTAATACtctctc                                                                                                                                                                                                                                   >  7:1345881/1‑149 (MQ=255)
                                                                                                                                                       gTTTCCNCACTGGTGNNAATTGAACTGATATTTAGCGCCGNCATCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActct                                                                                                                                                                                                                                      <  3:63695/145‑1 (MQ=255)
                                                                                                                                                         ttCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGc                                                                                                                                                                                                                                >  5:814445/1‑149 (MQ=255)
                                                                                                                                                               acTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                                                                                                                                                                                          <  7:27259/149‑1 (MQ=255)
                                                                                                                                                                  ggtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtg                                                                                                                                                                                                                       <  6:330495/149‑1 (MQ=255)
                                                                                                                                                                    tggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                                                                                                                                     >  6:1091923/1‑149 (MQ=255)
                                                                                                                                                                      ggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                                                                                                                                   <  8:246527/149‑1 (MQ=255)
                                                                                                                                                                       gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                                                                                                                                                                                  <  6:136523/149‑1 (MQ=255)
                                                                                                                                                                       gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGCTGTa                                                                                                                                                                                                                  <  5:1462344/149‑1 (MQ=255)
                                                                                                                                                                         aTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                                                                                                                                                                                <  5:291175/149‑1 (MQ=255)
                                                                                                                                                                               cTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                                                                                                                                                                                          <  8:1593818/149‑1 (MQ=255)
                                                                                                                                                                                tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGa                                                                                                                                                                                                                                                     >  6:496915/1‑105 (MQ=255)
                                                                                                                                                                                tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTg                                                                                                                                                                                                         >  5:543245/1‑149 (MQ=255)
                                                                                                                                                                                 gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgc                                                                                                                                                                                                        <  5:899781/149‑1 (MQ=255)
                                                                                                                                                                                     tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCAc                                                                                                                                                                                                    >  8:832552/1‑149 (MQ=255)
                                                                                                                                                                                         gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                                                                                                                                                                                       <  7:1616674/94‑1 (MQ=38)
                                                                                                                                                                                           gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                                                                                                                                                                                <  7:834326/131‑1 (MQ=255)
                                                                                                                                                                                            ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAtt                                                                                                                                                                                             <  8:1021025/149‑1 (MQ=255)
                                                                                                                                                                                             cgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAt                                                                                                                                                                                                                                                    >  3:36261/1‑93 (MQ=255)
                                                                                                                                                                                               ccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTa                                                                                                                                                                                                                                                        >  4:523396/1‑87 (MQ=255)
                                                                                                                                                                                                cAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                                                                                                                                                                                         <  6:1213143/149‑1 (MQ=255)
                                                                                                                                                                                                 aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaat                                                                                                                                                                                        <  7:1032374/149‑1 (MQ=255)
                                                                                                                                                                                                 aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAaat                                                                                                                                                                                        >  5:446247/1‑149 (MQ=255)
                                                                                                                                                                                                    cTGTTCAGCTTGGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACGTGAGGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGAACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCAGGGCTGATATGTTCCGTAGGCAGGTTTAATGTTTTAAAAGTg                                                       <  2:385465/275‑1 (MQ=255)
                                                                                                                                                                                                       ttCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                                                                                                                                                  >  8:1621970/1‑149 (MQ=255)
                                                                                                                                                                                                       ttCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                                                                                                                                                                                  <  5:1307950/149‑1 (MQ=255)
                                                                                                                                                                                                        tCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAcc                                                                                                                                                                                 >  8:1008628/1‑149 (MQ=255)
                                                                                                                                                                                                         cAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCt                                                                                                                                                                                <  6:977958/149‑1 (MQ=255)
                                                                                                                                                                                                            cTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCt                                                                                                                                                                             >  1:68036/1‑149 (MQ=255)
                                                                                                                                                                                                                 ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGa                                                                                                                                                                        >  6:178051/1‑149 (MQ=255)
                                                                                                                                                                                                                 ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGa                                                                                                                                                                        <  5:1271405/149‑1 (MQ=255)
                                                                                                                                                                                                                 ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGa                                                                                                                                                                        <  6:809503/149‑1 (MQ=255)
                                                                                                                                                                                                                  cAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCt                                                                                                                                                                                >  5:968336/1‑140 (MQ=255)
                                                                                                                                                                                                                          tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa                                                                                                                                                               <  6:1511763/149‑1 (MQ=255)
                                                                                                                                                                                                                            gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                                                                                                                                             >  8:549328/1‑149 (MQ=255)
                                                                                                                                                                                                                                 gcagcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                                                                                                                                   >  5:346597/1‑90 (MQ=255)
                                                                                                                                                                                                                                   agcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGaa                                                                                                                                                      <  8:205347/149‑1 (MQ=255)
                                                                                                                                                                                                                                   agcaATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGaa                                                                                                                                                      >  8:929230/1‑149 (MQ=255)
                                                                                                                                                                                                                                    gcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAAt                                                                                                                                                     >  7:513761/1‑149 (MQ=255)
                                                                                                                                                                                                                                     caATAATTCCCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                                                                                                                                     <  1:37743/164‑1 (MQ=255)
                                                                                                                                                                                                                                       ataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTc                                                                                                                                                  <  6:1539590/149‑1 (MQ=255)
                                                                                                                                                                                                                                           ttACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAtt                                                                                                                                                                                             <  8:769934/102‑1 (MQ=255)
                                                                                                                                                                                                                                           ttACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATc                                                                                                                                               <  5:208555/148‑1 (MQ=255)
                                                                                                                                                                                                                                              ccccGGTTGTCACCCGTATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAt                                                                                                                                           <  6:158612/149‑1 (MQ=255)
                                                                                                                                                                                                                                               cccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCATGGCTGATATTTTCCGTAGTCAGGTTTAATGTTTTAAAAGTGCTGTGGGAAAGTGAACAAAGAGTTCCGTAAGCGTTGATGCTAt            >  2:519523/1‑275 (MQ=255)
                                                                                                                                                                                                                                                 cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAtttt                                                                                                                                        <  5:1091923/149‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAtttt                                                                                                                                        >  8:125008/1‑149 (MQ=255)
                                                                                                                                                                                                                                                  ggTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttt                                                                                                                                       <  6:377320/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   gTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAACCAt                                                                                                      >  2:715318/1‑181 (MQ=255)
                                                                                                                                                                                                                                                     tGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCATGGCTGATATTTTCCGTAGTCAGGTTTAATGTTTTAAAAGTGCTGTGGGAAAGTGAACAAAGAGTTCCGTAAGCGTTGATGCTATGGGCGGTTaa  <  1:125218/279‑1 (MQ=255)
                                                                                                                                                                                                                                                          cccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTACTTTTTTTCActct                                                                                                                               <  6:280921/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           tcGGATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa                                                                                                                              >  8:36818/2‑149 (MQ=255)
                                                                                                                                                                                                                                                           ccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGt                                                                                                                                                                          <  8:1377847/105‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTa                                                                                                                              >  5:1206629/1‑149 (MQ=255)
                                                                                                                                                                                                                                                            cGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTAt                                                                                                                             <  7:218516/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattat                                                                                                                                                                    >  7:1392822/1‑107 (MQ=39)
                                                                                                                                                                                                                                                                tCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                                                                                                                                   >  7:677345/1‑59 (MQ=255)
                                                                                                                                                                                                                                                                tCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                                                                                                                                   <  8:677345/59‑1 (MQ=255)
                                                                                                                                                                                                                                                                  aTAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCaaa                                                                                                                       >  7:933306/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   tAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAt                                                                                                                      >  8:1425620/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     gTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa                                                                                                                    >  5:515061/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg                                                                                                                   >  7:760221/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        aCTTGATGTGACTATGTTCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCa                                                                                                                                   <  7:1014040/131‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGcc                                                                                                                 >  5:6732/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         cTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCATGGc                                                                                             <  3:23761/168‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ttGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCaaa                                                                                                                       >  5:369143/1‑141 (MQ=255)
                                                                                                                                                                                                                                                                          ttGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCAt                                                                                                               >  5:7038/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              tgtgACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCa                                                                                                           >  6:1314821/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              tgtgACTATGATCCGATTAAGACTCTCGCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCa                                                                                                           >  6:1681268/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                             
TTTGTTCTGTGTTGCAGGGGTTATTCGCCTGATGAGTTGGTATCGTCAGCGTTCAATGAAAGGCAGCTGGCTACAGCTCGTTATTGGTGTGCTGGATATCGTCATTGCCTGGATATTCCTTGGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAAACATTATCATGGCTGATATTTTCCGTAGTCAGGTTTAATGTTTTAAAAGTGCTGTGGGAAAGTGAACAAAGAGTTCCGTAAGCGTTGATGCTATGGGCGGTTAA  >  NC_000913/3657333‑3657856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: