Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,774,222 A→G 5.4% intergenic (+28/‑202) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,774,2220AG 5.4% 4842.2 / 157.8 1429intergenic (+28/‑202)mtlA/mtlDmannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (729/617);  new base G (50/27);  total (785/644)
Fisher's exact test for biased strand distribution p-value = 7.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01

Only 100 of 1453 total aligned reads displayed.

TACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAAAAACATTGATGAAGGTTAATACTATGAAAGCATTACATTTTGGCGCAGGTAATATCGGTCGTGGCTTTATCGGTAAACTGCTGGCAGACGCGGGTA  >  NC_000913/3773945‑3774496
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                   
tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGAGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCCGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCGGGCAGGTCGTAAGTAATCCAATCCCACACTCTCCACATGGAGAAg                                                                                                                                                                                                                                                                                    >  1:204540/1‑279 (MQ=255)
          gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTTCGCTTCGGTGAAGAAGAAGATGACATTTCCCGTCTGGTGCTTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCTCTGGATGATGAGTCCGTCATCCAGCGTCTGGCACACACCACCAGCGTGGTTGCAGTGCTGGAATTGCTGGCAGGTCGTAAGTACTCCAATACCACACTCCCCCCACGGAGAAggagcg                                                                                                                                                                                                                                                                               >  4:553858/1‑270 (MQ=255)
                            aaaaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc                                                                                                                                                                                                                                                             >  2:22217/1‑274 (MQ=255)
                                                                        cGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGc                                                                                                                                                                                                                                                        >  2:284933/1‑235 (MQ=255)
                                                                                         aCATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcg                                                                                                                                                                                                                                                              <  2:559084/212‑1 (MQ=255)
                                                                                                                       cccGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCCCGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTg                                                                                                                                                                >  2:494467/1‑275 (MQ=255)
                                                                                                                                  gAGCACATTCAGGTTATCACCAGCCTGATCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACGAGAGTGGATGATGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                                                                                                                                                    >  6:1227891/1‑149 (MQ=255)
                                                                                                                                  gAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAg                                                                                                                                                                                                                                                                                    <  6:1293871/149‑1 (MQ=255)
                                                                                                                                    gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                                                                                                                                                                                  >  8:1386669/1‑149 (MQ=255)
                                                                                                                                    gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGt                                                                                                                                                                                                                                                                                  >  8:1645292/1‑149 (MQ=255)
                                                                                                                                      acaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                                                                                                                                                                                <  6:977924/149‑1 (MQ=255)
                                                                                                                                       caTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAGGGTggg                                                                                                                                                                                                                                                                               <  6:1548234/149‑1 (MQ=255)
                                                                                                                                        aTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgggg                                                                                                                                                                                                                                                                              <  6:711597/149‑1 (MQ=255)
                                                                                                                                         ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGt                                                                                                                                                                                                                                                                             >  7:808335/1‑149 (MQ=255)
                                                                                                                                         ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGt                                                                                                                                                                                                                                                                             >  8:210298/1‑149 (MQ=255)
                                                                                                                                          tCAGGTTATCACCAGCCTAACCAATGCACTGGAGGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGA                                                                                                                                              <  2:626240‑M1/274‑1 (MQ=255)
                                                                                                                                             ggTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                                                                                                                                                                         <  6:435262/149‑1 (MQ=255)
                                                                                                                                              gttactcccAGCCTTACCAAGGAACTGGATGATGAGCCCGTCATCGGGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGtt                                                                                                                                         <  4:245160/268‑1 (MQ=255)
                                                                                                                                               ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGGGGGGTTAATTg                                                                                                                                                                                                                                                                       >  7:1688373/1‑149 (MQ=255)
                                                                                                                                                  tCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                                                                                                                                                                                <  5:818919/137‑1 (MQ=255)
                                                                                                                                                    aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                                                                                                                                                  >  6:253660/1‑149 (MQ=255)
                                                                                                                                                    aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                                                                                                                                                  >  7:897692/1‑149 (MQ=255)
                                                                                                                                                    aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                                                                                                                                                  >  8:790828/1‑149 (MQ=255)
                                                                                                                                                    aCCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                                                                                                                                                  >  5:321009/1‑149 (MQ=255)
                                                                                                                                                     ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt                                                                                                                                                                                                                                                                 >  6:910491/1‑149 (MQ=255)
                                                                                                                                                     ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt                                                                                                                                                                                                                                                                 >  7:1546770/1‑149 (MQ=255)
                                                                                                                                                     ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGATCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGAt                                                                                                                                                                                                                                                                 >  7:1597324/1‑149 (MQ=255)
                                                                                                                                                      cAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCa                                                                                                                                                                                                              <  4:32112/199‑1 (MQ=255)
                                                                                                                                                        gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTa                                                                                                                                                                                                                                                     >  2:346649/1‑158 (MQ=255)
                                                                                                                                                        gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGNGGGGTTAATTGCCTGATGCGCTACGCTTATCCGGCCTACAGGATGCATCACAATTTTTTGAATTTGCACNTTCTTTTAGCCCGGATAAGGCCCTTAAGCCGCAACACGCGCCGCCNCNCCCCCCCCGGAGAGGGttggggtgggggcaa                                                                                                                            >  3:76199‑M1/1‑263 (MQ=255)
                                                                                                                                                          cTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                                                                                                                                                                                >  6:534361/1‑129 (MQ=255)
                                                                                                                                                          cTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCt                                                                                                                                                                                                                                                            >  7:447150/1‑149 (MQ=255)
                                                                                                                                                            gACCAATGCACTGGATGATGAGTCCGTCATCGAGCGGCTGGCACCCCCCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATCGAGAAGGCGGGGTTAATTGCCTGATGTGCTATGCTTATCAGTCCTACAGGATGCATCACAATTTGTTGACTTTGCACTTTATTGTAGGCTTGATAAGGCGCTTACTCCGCATCCGGCGACTACCCTACACCACCGGAGAGGGTTTggtgtagggaca                                                                                                                            >  4:388429/1‑264 (MQ=255)
                                                                                                                                                               cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCt                                                                                                                                                                                                                                                       >  5:631319/1‑149 (MQ=255)
                                                                                                                                                                aaTGCACTGGACGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCtt                                                                                                                                                                                                                                                      >  5:1276174/1‑149 (MQ=255)
                                                                                                                                                                       tggctcatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAggt                                                                                                                                                                                                                                               <  8:1455718/143‑2 (MQ=255)
                                                                                                                                                                       tGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                                                                                                                                                               >  6:806455/1‑149 (MQ=255)
                                                                                                                                                                       tGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                                                                                                                                                               <  7:313047/149‑1 (MQ=255)
                                                                                                                                                                       tGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                                                                                                                                                               >  5:921770/1‑149 (MQ=255)
                                                                                                                                                                        ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGcc                                                                                                                                                                                                                                              >  5:697328/1‑149 (MQ=255)
                                                                                                                                                                           tgatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTAc                                                                                                                                                                                                                                           <  7:729591/149‑1 (MQ=255)
                                                                                                                                                                            gatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                                                                                                                                                                          <  5:1095173/149‑1 (MQ=255)
                                                                                                                                                                                 gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATg                                                                                                                                                                                                                                     >  8:1172552/1‑149 (MQ=255)
                                                                                                                                                                                     gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATc                                                                                                                                                                                                                                 >  5:979316/1‑149 (MQ=255)
                                                                                                                                                                                         tCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACaa                                                                                                                                                                                                                             >  7:980406/1‑149 (MQ=255)
                                                                                                                                                                                           gAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAtt                                                                                                                                                                                                                           >  8:1491016/1‑149 (MQ=255)
                                                                                                                                                                                            aGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCAACACAAttt                                                                                                                                                                                                                          >  7:1178639/1‑149 (MQ=255)
                                                                                                                                                                                             gCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                                                                                                                                                                                                                                                                        <  8:1084268/102‑1 (MQ=255)
                                                                                                                                                                                                tCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                                                                                                                                                                                                                                                                      <  6:1380/101‑1 (MQ=255)
                                                                                                                                                                                                tCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGA                                                                                                                                                <  4:640481‑M1/218‑1 (MQ=255)
                                                                                                                                                                                                      acacacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTg                                                                                                                                                                                                                >  8:1331556/1‑149 (MQ=255)
                                                                                                                                                                                                        acacCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCa                                                                                                                                                                                                              >  5:1634609/1‑149 (MQ=255)
                                                                                                                                                                                                          accaccAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                                                                                                                                                                                    >  5:1200792/1‑125 (MQ=255)
                                                                                                                                                                                                             accaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                                                                                                                                                             <  8:73091/113‑1 (MQ=255)
                                                                                                                                                                                                              ccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAACGCCTCACCCCAGCCCTCTCGGGTAAAAACATTGATGAAGGTTACTACTATGaaa                                                                     >  1:293017/1‑279 (MQ=255)
                                                                                                                                                                                                                  cGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGCTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGCGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAACAACATTGCTGAAGGCTAATACTATGaa                                                                      >  2:414406/1‑274 (MQ=255)
                                                                                                                                                                                                                    tGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTg                                                                                                                                                                                                                                                                   >  3:248537/1‑84 (MQ=255)
                                                                                                                                                                                                                       aTGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCg                                                                                                                                                                                               >  5:1455510/1‑149 (MQ=255)
                                                                                                                                                                                                                        tGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGaa                                                                                                                             <  2:103940/213‑1 (MQ=255)
                                                                                                                                                                                                                          aaGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                                                                                                                                                                          <  4:738889/103‑1 (MQ=255)
                                                                                                                                                                                                                            gTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATaa                                                                                                                                                                                          <  6:1177659/149‑1 (MQ=255)
                                                                                                                                                                                                                               cTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgc                                                                                                                                                                                       >  8:645673/1‑149 (MQ=255)
                                                                                                                                                                                                                                tGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGa                                                                                                                                                                                                                                                                  <  8:46635/73‑1 (MQ=255)
                                                                                                                                                                                                                                 ggAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgcgc                                                                                                                                                                                     >  5:37788/1‑149 (MQ=255)
                                                                                                                                                                                                                                   aaCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCtt                                                                                                                                                                                   >  6:1661912/1‑149 (MQ=255)
                                                                                                                                                                                                                                      tgctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCtt                                                                                                                                                                                                                                                      >  8:1247474/1‑79 (MQ=255)
                                                                                                                                                                                                                                      tgctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcg                                                                                                                                                                                >  8:1579891/1‑149 (MQ=255)
                                                                                                                                                                                                                                            cAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCAt                                                                                                                                                                          <  7:225879/149‑1 (MQ=255)
                                                                                                                                                                                                                                             aGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                                                                                                                                                                       <  6:1476387/103‑1 (MQ=255)
                                                                                                                                                                                                                                             aGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATc                                                                                                                                                                         >  8:401021/1‑149 (MQ=255)
                                                                                                                                                                                                                                               gTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCg                                                                                                                                                                       >  7:1479321/1‑149 (MQ=255)
                                                                                                                                                                                                                                                tCGTAAGTAATCCAGTCCCACTCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTAAGCTTATCAGGCCTACAGGAAGCATCTCAATTTGTTGAATTTGCACGTTCGTGTAGGCCGGATAAGGCGCTTACGCCGCATCCgg                                                                                                                                                                      <  5:21463/149‑1 (MQ=25)
                                                                                                                                                                                                                                                   taagtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGc                                                                                                                                                                                                               >  5:570779/1‑105 (MQ=255)
                                                                                                                                                                                                                                                   taagtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGgcgc                                                                                                                                                                  >  7:1414581/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       taaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGcc                                                                                                                                                              >  7:1350286/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        aatccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGccc                                                                                                                                                             <  6:65266/149‑1 (MQ=255)
                                                                                                                                                                                                                                                          tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                                                                                                                                                           >  5:774620/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                                                                                                                                                           >  5:1388474/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                                                                                                                                                           >  5:463111/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                                                                                                                                                           >  7:625983/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           ccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCctc                                                                                                                                                          >  6:596883/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             aatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGccc                                                                                                                                                             <  7:1267175/144‑1 (MQ=255)
                                                                                                                                                                                                                                                              atccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGgcg                                                                                                                                                                    <  4:488394/137‑1 (MQ=255)
                                                                                                                                                                                                                                                               tccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGACCCTctccg                                                                                                                                                      >  7:538133/1‑148 (MQ=255)
                                                                                                                                                                                                                                                               cCCCACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcct                                                                                                                                                      >  7:1035008‑M2/2‑149 (MQ=255)
                                                                                                                                                                                                                                                                cccACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcctc                                                                                                                                                     >  8:55297/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  cACCTTCTCCATGTGGAGAGGGTGGGATTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCAc                                                                                                                                                   >  6:108238/1‑149 (MQ=25)
                                                                                                                                                                                                                                                                  cACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTctcc                                                                                                                                                       >  6:191573/1‑145 (MQ=255)
                                                                                                                                                                                                                                                                    cccTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAAAAACAtt                                                                                           >  2:743531/1‑203 (MQ=255)
                                                                                                                                                                                                                                                                       tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAAAAACATTGATGAAGGTTAATACTATGAAAGCATTACATTTTGGCGCAGGTAATATCGGTCGTGGCTTTATCGGTAAACTGCTGGCa            <  1:106586/279‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGA                                                                                                                                              >  5:1527480‑M1/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGA                                                                                                                                              <  7:385185‑M1/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGA                                                                                                                                              <  8:328928‑M1/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                        ctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCAc                                                                                                                                                   >  4:96198/1‑143 (MQ=255)
                                                                                                                                                                                                                                                                        ctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAG                                                                                                                                             <  7:1113334‑M1/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gccACATGGAGAAGTTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcgcc                                                                                                                                                                              <  1:65467/115‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ccACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGG                                                                                                                                           <  6:347422‑M1/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           cacaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGt                                                                                                                                          >  8:887756/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                            acaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAG                                                                                                                                               <  2:96199‑M1/143‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gaAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg                                                                                                                                      <  5:172397/145‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                   
TACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGGGTGAGGGAAAAGCCTCACCCCAGCCCTCTCGGGTAAAAACATTGATGAAGGTTAATACTATGAAAGCATTACATTTTGGCGCAGGTAATATCGGTCGTGGCTTTATCGGTAAACTGCTGGCAGACGCGGGTA  >  NC_000913/3773945‑3774496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: