Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 543,091 G→C 5.6% intergenic (+58/+170) glxK → / ← allE glycerate kinase II/S‑ureidoglycine aminohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913543,0910GC 5.6% 967.0 / 36.8 276intergenic (+58/+170)glxK/allEglycerate kinase II/S‑ureidoglycine aminohydrolase
Reads supporting (aligned to +/- strand):  ref base G (136/122);  new base C (7/9);  total (143/133)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-04

Only 100 of 278 total aligned reads displayed.

CGAAGCAGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCT  >  NC_000913/542822‑543340
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                         
tggaggaGTTTTATGTAGCGGTGTGTGGGATGGCTGGCGTATTTGGTGATGGTGTGTGTGTGGGGGACCAGTATGGCATTGACGCGGTATTCAGCATTTTGCCTCGGCTGGCACCTTTGGCCGAAGTGCTCGCCAGCGGTGAAACCAAGCTCTTCAACAGTGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGc                                                                                                                                                                                                                                                       <  4:253440/268‑1 (MQ=255)
      aGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATg                                                                                                                                                                                                                                                 <  2:486244/274‑1 (MQ=255)
                                           gggTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCCCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAACCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTTCAGTACCCCAGCCGGCAGCGGCATGAGTAACCTATCCATCCTGAAAATTCCGCAACTTCtaga                                                                                                                                                                                                           >  4:195368/1‑273 (MQ=255)
                                                 tGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTACAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCCATTTATATCGCATAAGAGTGCAGTACTCATGCCGGTTGCGGCTAGAGTACCCTAACCTTCCTTAAAATCGCCAAAATTCTATTTATTg                                                                                                                                                                                                      >  4:543922/1‑274 (MQ=255)
                                                                  aCCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                                                                                                                                  <  2:616200/213‑1 (MQ=255)
                                                                                      gTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCa                                                                                                                                                                  <  2:195371/273‑1 (MQ=255)
                                                                                      gTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAgg                                                                                                                                                                >  2:388815/1‑275 (MQ=255)
                                                                                                         gTCTTGCACCTTTAGCCGAAGTGCGCGCCAGGGGGGAAAACAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGcc                                                                                                                                             <  4:399775/275‑1 (MQ=255)
                                                                                                                                  cGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATc                                                                                                                                                                                                                         >  2:435438/1‑174 (MQ=255)
                                                                                                                                    ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                                                                                                                                <  7:65217/149‑1 (MQ=255)
                                                                                                                                    ccAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                                                                                                                                <  6:1478669/149‑1 (MQ=255)
                                                                                                                                         ggTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAcc                                                                                                                                                                                                                                           >  7:496176/1‑149 (MQ=255)
                                                                                                                                               aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                                                                                                                                     >  6:1075895/1‑149 (MQ=255)
                                                                                                                                               aCCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtcc                                                                                                                                                                                                                                     >  5:130163/1‑149 (MQ=255)
                                                                                                                                                ccAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGc                                                                                                                                                                                                                                                    <  7:1297611/133‑1 (MQ=255)
                                                                                                                                                  aagctctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTAATGCCGGATGCGGCATGAGTACCATAtccttc                                                                                                                                                                                                                                  >  8:1130566/4‑149 (MQ=255)
                                                                                                                                                   aTCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATAtccttcc                                                                                                                                                                                                                                 <  6:574733/149‑1 (MQ=255)
                                                                                                                                                    tctcTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCa                                                                                                                                                                                                                                          <  8:58629/139‑1 (MQ=255)
                                                                                                                                                      tctTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGa                                                                                                                                                                                                                              >  5:905079/1‑149 (MQ=255)
                                                                                                                                                       ctTCAAAAGGGCGCGAAAAATTGCCTGGGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAg                                                                                                <  4:127996/274‑1 (MQ=255)
                                                                                                                                                        ttCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGaaa                                                                                                                                                                                                                            >  7:341528/1‑149 (MQ=255)
                                                                                                                                                          cAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAACCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCCCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAACGAAGCGCATCATGCAATGTTACAAAAAAAGCAACGGATAAAAACGTTGCAAAATCCAACATTACTAAAATAAATAAGGcag                                                                                            >  4:304178/1‑273 (MQ=255)
                                                                                                                                                           aaCAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATc                                                                                                                                                                                                                         <  6:259470/149‑1 (MQ=255)
                                                                                                                                                                  cgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtatata                                                                                                                                                                                                         >  4:41373/1‑158 (MQ=255)
                                                                                                                                                                  cgcgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACtt                                                                                >  1:155969/1‑279 (MQ=255)
                                                                                                                                                                    cgAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCgcgc                                                                                                                                                                                                                     <  2:329109/144‑1 (MQ=255)
                                                                                                                                                                     gAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGAg                                                                                                                                                                                                                                               >  8:1330484/1‑117 (MQ=255)
                                                                                                                                                                      aaaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                                                                                                                                     <  2:41373/158‑1 (MQ=255)
                                                                                                                                                                       aaTATTGCCTGCGCCATTAATATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCta                                                                                                                                                                                                             <  7:1386299/149‑1 (MQ=255)
                                                                                                                                                                       aaTATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCATACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATtata                                                                                                                                                                                                             <  8:1498725/149‑1 (MQ=255)
                                                                                                                                                                          atTGCCTGCGCCATTAAAAAAGGTCAGGGAATTAAAAACTAAGCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtatac                                                                                                                                                                                                          >  7:993528/1‑148 (MQ=255)
                                                                                                                                                                           ttGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtatata                                                                                                                                                                                                         >  8:695035/1‑149 (MQ=255)
                                                                                                                                                                           ttGCCTGCGCCATTAAAATAGGTCAGGGAATCAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGACGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCtatata                                                                                                                                                                                                         >  8:1033874/1‑149 (MQ=255)
                                                                                                                                                                               cTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCta                                                                                                                                                                                                             >  5:206646/1‑141 (MQ=255)
                                                                                                                                                                               cTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                                                                                                                                     >  6:133158/1‑149 (MQ=255)
                                                                                                                                                                               cTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                                                                                                                                     <  5:1533614/149‑1 (MQ=255)
                                                                                                                                                                                 gcgcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGa                                                                                                                                                                                                                                                >  1:81402/1‑104 (MQ=255)
                                                                                                                                                                                  cgcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCaga                                                                                                                                                                                                  <  6:1298497/149‑1 (MQ=255)
                                                                                                                                                                                   gcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAc                                                                                                                                                                                                                                            <  6:989037/106‑1 (MQ=255)
                                                                                                                                                                                   gcCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTAc                                                                                                                                                                                                                                            >  5:989037/1‑106 (MQ=255)
                                                                                                                                                                                     cATTAAAATAGGTCAGGGAATTAAAAACTAACCATTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTTCAGAGAt                                                                                                                                                                                               >  5:807062/1‑149 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGTCAGGGAATTAAAATCTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCaga                                                                                                                                                                                                  <  5:60632/145‑1 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                                                                                                                              >  5:402578/1‑149 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                                                                                                                              >  5:54144/1‑149 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                                                                                                                              >  5:150670/1‑149 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGCATCCGGCATGa                                                                                                                                                                                                                                                >  8:844895/1‑99 (MQ=255)
                                                                                                                                                                                      aTTAAAATAGGACAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATc                                                                                                                                                                                              >  5:1276764/1‑149 (MQ=255)
                                                                                                                                                                                       ttAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCa                                                                                                                                                                                             >  6:639166/1‑149 (MQ=255)
                                                                                                                                                                                        tAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCAt                                                                                                                                                                                            <  5:1422576/149‑1 (MQ=255)
                                                                                                                                                                                            aTAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAg                                                                                                                                                                                        <  7:306669/149‑1 (MQ=255)
                                                                                                                                                                                              aGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGc                                                                                                                                                                                      <  6:777056/149‑1 (MQ=255)
                                                                                                                                                                                               ggTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATc                                                                                                                                                                                                                         >  4:784358/1‑113 (MQ=255)
                                                                                                                                                                                                gTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCt                                                                                                                                                                                    >  8:126534/1‑149 (MQ=255)
                                                                                                                                                                                                 tCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                                                                                                                                     <  5:1616759/131‑1 (MQ=255)
                                                                                                                                                                                                 tCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGc                                                                                                                                                                                                     >  6:1616759/1‑131 (MQ=255)
                                                                                                                                                                                                 tCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCg                                                  >  1:187317/1‑278 (MQ=255)
                                                                                                                                                                                                  cAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGa                                                                                                                                                                                  >  7:268176/1‑149 (MQ=255)
                                                                                                                                                                                                         ttAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATaagcga                                                                                                                                                                           >  8:1417324/1‑149 (MQ=255)
                                                                                                                                                                                                          tAAAAACTAACCCTTACGTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTAAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACa                                                                                                                 <  1:119260/206‑1 (MQ=255)
                                                                                                                                                                                                          tAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCCCGGTTACAATCTTTCGatgaaa                                            >  2:552335/1‑270 (MQ=255)
                                                                                                                                                                                                                 tAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATc                                                                                                                                                                   <  7:774441/149‑1 (MQ=255)
                                                                                                                                                                                                                   aCCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCt                                                                                                                                                                                                              <  1:23022/104‑1 (MQ=255)
                                                                                                                                                                                                                   aCCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCt                                                                                                                                                                                                              >  3:23022/1‑104 (MQ=255)
                                                                                                                                                                                                                      cTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTa                                                                                                                                                                                                                                             <  7:1475347/70‑1 (MQ=255)
                                                                                                                                                                                                                       ttACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCaa                                                                                                                                                             >  8:319057/1‑149 (MQ=255)
                                                                                                                                                                                                                             ttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTcacc                         <  2:523257/275‑1 (MQ=255)
                                                                                                                                                                                                                             ttAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCAGGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTcacc                         <  2:304182/275‑1 (MQ=255)
                                                                                                                                                                                                                                 ctcttaTGCGATATAAATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaa                                                                                                                                                   >  8:696739/6‑149 (MQ=255)
                                                                                                                                                                                                                                 aGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACaaaa                                                                                                                                                   >  8:1679616/1‑149 (MQ=255)
                                                                                                                                                                                                                                     gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAg                                                                                                                                               >  7:932881/1‑149 (MQ=255)
                                                                                                                                                                                                                                     gATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACg                      <  4:600241/270‑1 (MQ=255)
                                                                                                                                                                                                                                      aTGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGc                                                                                                                                              <  7:1033874/149‑1 (MQ=255)
                                                                                                                                                                                                                                         cGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCAt                                                                                                                                                                                                                                                  <  4:27864/46‑1 (MQ=255)
                                                                                                                                                                                                                                           aTTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACgg                                                                                                                                         >  8:891189/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    gCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCa                                                                                                                                                                                                    <  3:180047/81‑1 (MQ=255)
                                                                                                                                                                                                                                                    gCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCa                                                                                                                                                                                                    <  6:371/81‑1 (MQ=255)
                                                                                                                                                                                                                                                     cATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCt  >  4:449780/1‑274 (MQ=255)
                                                                                                                                                                                                                                                      aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTTTATACCGTg                                                                                                                              <  8:496176/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                                                                                                                              <  5:1143934/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      aTAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTaaaaa                                                                                                       >  2:25942/1‑172 (MQ=255)
                                                                                                                                                                                                                                                       tAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTTTATACCGTgg                                                                                                                             <  6:1692833/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGc                                                                                                                            >  7:415707/1‑149 (MQ=255)
                                                                                                                                                                                                                                                         agagTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTTTATACCGTGGct                                                                                                                           <  7:857780/149‑2 (MQ=255)
                                                                                                                                                                                                                                                          gagTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGNATCAGGCAATGTTACAAAAAAAANNACGGTATANACCGTGGCAAAATCCAACATAGCTAAAAATANACAGGCGAg                                                                                           >  3:65388/1‑180 (MQ=255)
                                                                                                                                                                                                                                                             tGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAgaga                                                                                                                                                                                                <  1:49242/76‑1 (MQ=255)
                                                                                                                                                                                                                                                             tGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCa                                                                                                                                                                                             >  2:140994/1‑79 (MQ=255)
                                                                                                                                                                                                                                                             tGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCa                                                                                                 >  2:465958/1‑171 (MQ=255)
                                                                                                                                                                                                                                                              gCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTTTATACCGTGGctttttt                                                                                                                      <  6:674545/149‑7 (MQ=255)
                                                                                                                                                                                                                                                               cAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATcc                                                                                                                     <  8:1589911/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTa                                                                                                                                                                                         <  4:140992/79‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCaa                                                                                                                   >  8:379934/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTg                                                                                                                                                                                   >  7:1627816/1‑81 (MQ=255)
                                                                                                                                                                                                                                                                     tCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATa                                                                                                               >  7:703168/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTg                                                                                                                              >  8:749004/1‑132 (MQ=255)
                                                                                                                                                                                                                                                                       aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAAt                                                                                                                       >  7:1524548/1‑139 (MQ=255)
                                                                                                                                                                                                                                                                       aTGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGc                                                                                                             >  5:752710/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                       aTGCCGCATCCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGc                                                                                                             >  6:1157022/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAGATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCt                                                                                                            >  6:317784/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        tGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAgaga                                                                                                                                                                                                <  2:715101/65‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAaataat                                                                                                   <  4:47689/153‑1 (MQ=255)
                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                         
CGAAGCAGTTTAATGTACCGGTGATTGGGATTGCTGGCGTATTGGGTGATGGCGTGGAAGTGGTGCACCAGTACGGCATTGACGCGGTATTCAGCATTTTGCCTCGTCTGGCACCTTTAGCCGAAGTGCTCGCCAGCGGTGAAACCAATCTCTTCAACAGCGCGCGAAATATTGCCTGCGCCATTAAAATAGGTCAGGGAATTAAAAACTAACCCTTACCTTTAAAGCGGATGCGATTTATATCGCATAAGAGTGCAGTACTCATGCCGGATGCGGCATGAGTACCATATCCTTCCTGAAAATCGCGCAAATTCTATATATTGCAGAGATCATGTAGGCCTGATAAGCGAAGCGCATCAGGCAATGTTACAAAAAAAGCCACGGTATAAACCGTGGCAAAATCCAACATAGCTAAAAATAATCAGGCGAGTGGTATGACTTAAATCTCTACGTCGCGGTTACAATCTTTCGAGTAAATATAGCTGAACGCTTCACCACGCCCTACACCATAACCAGCCT  >  NC_000913/542822‑543340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: