Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,900,410 A→T 5.3% P56P (CCT→CCA yieL ← putative xylanase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,900,4100AT 5.3% 1067.4 / 35.0 303P56P (CCT→CCAyieLputative xylanase
Reads supporting (aligned to +/- strand):  ref base A (149/138);  new base T (11/5);  total (160/143)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-03

Only 100 of 308 total aligned reads displayed.

CAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCA  >  NC_000913/3900149‑3900677
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
                                                                      tGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaaa                                                                                                                                                                                                                                                                     <  2:76024/200‑1 (MQ=255)
                                                                                     ttCATTTCTGGGCATGTATAAATGCTACATACCNCATAAAACTTGAAAAAATACTCGTANCGGTTGCCTTTCAGGATAGGTGTGCGCAACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACNGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctgct                                                                                                                                                                       <  3:172176/278‑1 (MQ=255)
                                                                                          tGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACCTTTTTTGCCCCAGGGGCAAAGTAGCGAAANNNCACGCTg                                                                                                                                                                                                                                                  >  4:757310/1‑199 (MQ=255)
                                                                                          tGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctg                                                                                                                                                                         >  1:278016/1‑272 (MQ=255)
                                                                                                    gTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctgg                                                                                                                                                                                        >  1:102774/1‑247 (MQ=255)
                                                                                                     tATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACt                                                                                                                                                             <  2:189957/273‑1 (MQ=255)
                                                                                                           tgctgcGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACAt                                                                                                                                                                                                                         <  3:124634/207‑1 (MQ=255)
                                                                                                                cGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                                                                                                                                             >  4:339001/1‑182 (MQ=255)
                                                                                                                 gTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCa                                                                                                                                                                                                                                                                             >  7:622209/1‑149 (MQ=255)
                                                                                                                  tACACCATTAACATCGAAAAAATACTCGTACTGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGATTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAg                                                                                                                                                                                                                                                                            <  7:1044570/149‑1 (MQ=255)
                                                                                                                   acacCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAgg                                                                                                                                                                                                                                                                           <  8:1465784/149‑1 (MQ=255)
                                                                                                                    cacCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                                                                                                                                                                                                  <  4:274778/189‑1 (MQ=255)
                                                                                                                    cacCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctgct                                                                                                                                                                       <  2:440029/248‑1 (MQ=255)
                                                                                                                         tCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaaa                                                                                                                                                                                                                                                                     >  8:454444/1‑149 (MQ=255)
                                                                                                                          cAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt                                                                                                                                       >  2:278750/1‑274 (MQ=255)
                                                                                                                              aTTGAAAAAATACTCGTACAGGTTGCCTTTCATGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCATGGGCAAAGTAGc                                                                                                                                                                                                                                                                >  7:811155/1‑149 (MQ=255)
                                                                                                                               ttGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCg                                                                                                                                                                                                                                                               <  5:293143/149‑1 (MQ=255)
                                                                                                                                  aaaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                                                                                                                                            >  6:1160987/1‑149 (MQ=255)
                                                                                                                                   aaaaaTACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAg                                                                                                                                                                                                                                                           <  5:1050890/149‑1 (MQ=255)
                                                                                                                                          cTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGc                                                                                                                                                                                                                                                    <  5:951837/149‑1 (MQ=255)
                                                                                                                                           tCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCt                                                                                                                                                                                                                                                   >  6:1033347/1‑149 (MQ=255)
                                                                                                                                            cGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCNCAGGGGCNAAGNAGCTAANGGTCACGCTGTTATCGGTATTGAc                                                                                                                                                                                                                                    >  3:33663/1‑163 (MQ=255)
                                                                                                                                             gTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCTGGGGCaaaaaat                                                                                                                                                                                                                                                                 >  6:912984/1‑129 (MQ=255)
                                                                                                                                             gTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGt                                                                                                                                                                                                                                                 >  8:1619882/1‑149 (MQ=255)
                                                                                                                                                 aGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                                                                                                                                             >  8:602296/1‑149 (MQ=255)
                                                                                                                                                 aGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATc                                                                                                                                                                                                                                             <  6:198498/149‑1 (MQ=255)
                                                                                                                                                   gTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaaa                                                                                                                                                                                                                                                                     <  8:1132851/123‑1 (MQ=255)
                                                                                                                                                   gTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGt                                                                                                                                                                                                                                                         <  7:1613252/135‑1 (MQ=255)
                                                                                                                                                    ttGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTCCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGc                                                                                                                                                                                                                                          >  8:724770/1‑149 (MQ=255)
                                                                                                                                                     tGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTTATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAg                                                                                                                                                                                                                               >  3:249345/1‑159 (MQ=255)
                                                                                                                                                            cAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                                                                                                                                                                                                  <  6:749894/149‑1 (MQ=255)
                                                                                                                                                              ggATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                                                                                                                                                                                                <  7:425282/149‑1 (MQ=255)
                                                                                                                                                               gATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAg                                                                                                                                                                                                                               >  5:1388253/1‑149 (MQ=255)
                                                                                                                                                                    gtgtGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGaaa                                                                                                                                                                                                                                                            <  7:16765/115‑1 (MQ=255)
                                                                                                                                                                    gtgtGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGAAGCGaaa                                                                                                                                                                                                                                                            >  8:16765/1‑115 (MQ=255)
                                                                                                                                                                       tgCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgc                                                                                                                                                                           >  2:10176/1‑193 (MQ=255)
                                                                                                                                                                         cgcCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTg                                                                                                                                                                                                                     >  6:337654/1‑149 (MQ=255)
                                                                                                                                                                            cACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGttt                                                                                                                                                                                                                  >  6:443919/1‑149 (MQ=255)
                                                                                                                                                                             aCGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTc                                                                                                                                                                                                                 >  7:667402/1‑149 (MQ=255)
                                                                                                                                                                             aCGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTc                                                                                                                                                                                                                 >  7:1311511/1‑149 (MQ=255)
                                                                                                                                                                               gACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGa                                                                                                                                                                                                                                >  1:206643/1‑132 (MQ=255)
                                                                                                                                                                                aCCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCAcc                                                                                                                                                                                                              <  8:1285370/149‑1 (MQ=255)
                                                                                                                                                                                      cTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATa                                                                                                                                                                                                        <  5:996998/149‑1 (MQ=255)
                                                                                                                                                                                       tCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAg                                                                                                                                                                                                       >  6:1342324/1‑149 (MQ=255)
                                                                                                                                                                                        ccTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGt                                                                                                                                                                                                      <  8:1266447/149‑1 (MQ=255)
                                                                                                                                                                                        ccTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGt                                                                                                                                                                                                      <  5:1615085/149‑1 (MQ=255)
                                                                                                                                                                                         cTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTg                                                                                                                                                                                                     >  7:313421/1‑149 (MQ=255)
                                                                                                                                                                                          tGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCt                                                                                                                                                                                                                                  >  6:1629936/1‑119 (MQ=255)
                                                                                                                                                                                          tGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTgg                                                                                                                                                                                                    <  5:1160987/149‑1 (MQ=255)
                                                                                                                                                                                          tGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTgg                                                                                                                                                                                                    >  8:660976/1‑149 (MQ=255)
                                                                                                                                                                                                 tCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCgg                                                                                                                                                                                             >  5:1085486/1‑149 (MQ=255)
                                                                                                                                                                                                 tCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATGTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCgg                                                                                                                                                                                             <  5:1317543/149‑1 (MQ=255)
                                                                                                                                                                                                   tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCaaa                                                                                                                                                                                                                                                                     >  8:35071/1‑75 (MQ=255)
                                                                                                                                                                                                   tttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggc                                                                                                                                                                                           >  7:86849/1‑149 (MQ=255)
                                                                                                                                                                                                    ttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggct                                                                                                                                                                                          <  5:919752/149‑1 (MQ=255)
                                                                                                                                                                                                    ttGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAagca                                                                                                                       >  2:399602/1‑216 (MQ=255)
                                                                                                                                                                                                     tGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctg                                                                                                                                                                                         >  7:223790/1‑149 (MQ=255)
                                                                                                                                                                                                       gTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGGAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctggc                                                                                                                                                                                       <  5:1446223/149‑1 (MQ=255)
                                                                                                                                                                                                        tCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctggct                                                                                                                                                                                      >  6:284477/1‑149 (MQ=255)
                                                                                                                                                                                                        tCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggctggct                                                                                                                                                                                      >  5:688859/1‑149 (MQ=255)
                                                                                                                                                                                                         cATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATa                                                                                                                                                                                                        <  7:1531977/130‑1 (MQ=255)
                                                                                                                                                                                                          aTCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGctggctgg                                                                                                                                                                                    <  8:235016/149‑1 (MQ=255)
                                                                                                                                                                                                          aTCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGctggctgg                                                                                                                                                                                    <  5:866255/149‑1 (MQ=255)
                                                                                                                                                                                                           tCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCtggctggc                                                                                                                                                                                   >  5:616729/1‑149 (MQ=255)
                                                                                                                                                                                                               gTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCatat                                                                                                                                                                               >  6:1077472/1‑149 (MQ=255)
                                                                                                                                                                                                                 gAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGgctggctg                                                                                                                                                                                     <  7:488683/141‑1 (MQ=255)
                                                                                                                                                                                                                  aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCg                                                                                                                                                                            <  6:1388253/149‑1 (MQ=255)
                                                                                                                                                                                                                  aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCg                                                                                                                                                                            >  7:1091938/1‑149 (MQ=255)
                                                                                                                                                                                                                  aaTATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt                                                                                                                                       >  2:371446/1‑186 (MQ=255)
                                                                                                                                                                                                                    tatTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGggc                                                                                                                                                                                           <  8:516567/132‑1 (MQ=255)
                                                                                                                                                                                                                            ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGc                                                                                                                                                                  >  5:893375/1‑149 (MQ=255)
                                                                                                                                                                                                                            ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGc                                                                                                                                                                  >  7:1223857/1‑149 (MQ=255)
                                                                                                                                                                                                                            ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGc                                                                                                                                                                  >  5:1511036/1‑149 (MQ=255)
                                                                                                                                                                                                                            ggaaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGc                                                                                                                                                                  >  6:1548853/1‑149 (MQ=255)
                                                                                                                                                                                                                              aaccggaacACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACTTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCTGCTGCTATGGCGCACCGTTCCGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAAGTTTTATATTCATCGTAACATTCCTTTCCTTAAGTTCAATTACCACCAGATTTCAGCCTGGGCACCGCCGGCCAACTGgtc                                  >  4:278782/1‑275 (MQ=255)
                                                                                                                                                                                                                                     acaaCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGAATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGc                                                                                                                                                         <  7:974716/149‑1 (MQ=255)
                                                                                                                                                                                                                                             cacTACAGAGACATTTTTTGCCCCAGGGGCCAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATACGCTGCTATGGCCCACTGAGCAGAAATAc                                                                                                                                                 <  7:1007754/149‑1 (MQ=255)
                                                                                                                                                                                                                                              acTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATAcc                                                                                                                                                >  5:1699018/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 aCAGAGACATCTTTTGCCCCACGAGCAAAGTAGCGAAAAGACACGCTGTTATCGGCATTGGCCTGAGTCACATACTGATTCAACCGGATAGTGGGTGCCGGGCTGGCTGGCATACCCCCAGCTATGGCCCACTGAGCAGAAATACAGCt                                                                                                                                             <  8:622209/149‑1 (MQ=255)
                                                                                                                                                                                                                                                   agagACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATgg                                                                                                                                                                   >  6:807/1‑125 (MQ=255)
                                                                                                                                                                                                                                                   agagACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATgg                                                                                                                                                                   <  5:807/125‑1 (MQ=255)
                                                                                                                                                                                                                                                    gagaCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGc                                                                                                                                          <  8:223790/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      gaCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt                                                                                                                                       >  2:552564/1‑150 (MQ=255)
                                                                                                                                                                                                                                                       aCATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAAt                                                                                                                                       >  4:389171/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        gctactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATa                                                                                                                                      >  8:874206/6‑149 (MQ=255)
                                                                                                                                                                                                                                                          ttttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGttt                                                                                                                                                                                                                  >  4:412091/1‑71 (MQ=255)
                                                                                                                                                                                                                                                          tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGaaa                                                                                                                                                    >  8:1164714/4‑133 (MQ=255)
                                                                                                                                                                                                                                                          tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGaaa                                                                                                                                                    <  7:1164714/130‑1 (MQ=255)
                                                                                                                                                                                                                                                          tactttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGc                                                                                                                                    >  5:467595/4‑149 (MQ=255)
                                                                                                                                                                                                                                                           tttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCa                                                                                                       <  2:331693/177‑1 (MQ=255)
                                                                                                                                                                                                                                                           actttGCCCCTGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGcc                                                                                                                                   >  7:258498/3‑149 (MQ=255)
                                                                                                                                                                                                                                                            ttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCa                                                                                                                                                                                  >  2:617019/1‑101 (MQ=255)
                                                                                                                                                                                                                                                            ttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa                                                                                                                                  <  7:602296/149‑1 (MQ=255)
                                                                                                                                                                                                                                                            ttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCa                                                                                                                                  <  5:234014/149‑1 (MQ=255)
                                                                                                                                                                                                                                                               tGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATTGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTCTATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCa  >  2:70478/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                gCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATc                                                                                                                                                                              <  4:617015/101‑1 (MQ=255)
                                                                                                                                                                                                                                                                   ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCg                                                                                                                                                                            >  1:136862/1‑100 (MQ=255)
                                                                                                                                                                                                                                                                   ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACt                                                                                                                                                             >  4:478683/1‑115 (MQ=255)
                                                                                                                                                                                                                                                                   ccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaa                                                                                                                           >  6:949873/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    cAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTaaa                                                                                                                          <  6:150241/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                           
CAAATCACCATGCGCAACAGAACGCGTATCCAGATAACTGCCTGGCACCAGAATCATACTGGAGTTAACCTGGCGCTGAGGATTAGTCATTGCGGTGCCTGTATCAATGCTGCGTACACCATCAACATTGAAAAAATACTCGTACAGGTTGCCTTTCAGGATAGGTGTGCGCCACGACCAGACTCCTGCTTCGTCTTTGGTCATCGGGTGAATATTGTCCGGAACCGGAACACCCACCACTACAGAGACATTTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCTGGCAATAGCCAGCGTTAAAGCAGCAATTTTTATATTCATCGTAATATTCCTTTCATTAAGTTCCATTACCACCAGATTTCAGCCTGGGCACCGACAGCAAACTGGTCGTCTTTATTGTCTTCAAAGGTGAAGCCATCCA  >  NC_000913/3900149‑3900677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: