Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,056,539 T→A 5.2% intergenic (‑200/+86) glnL ← / ← glnA sensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,056,5390TA 5.2% 1028.7 / 32.5 292intergenic (‑200/+86)glnL/glnAsensory histidine kinase in two‑component regulatory system with GlnG/glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base T (142/134);  new base A (5/10);  total (148/144)
Fisher's exact test for biased strand distribution p-value = 1.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01

Only 100 of 300 total aligned reads displayed.

ATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGA  >  NC_000913/4056268‑4056812
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                 
                           cGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTTATGTGCTTGc                                                                                                                                                                                                                                                      >  2:266570/1‑274 (MQ=255)
                           cGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGAGTGATGTGCTTGc                                                                                                                                                                                                                                                      >  2:599214/1‑274 (MQ=255)
                           cGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGAAAAGACGCATTTGCTCCCCATCCGGccgtatttgcttcc                                                                                                                                                                                                                                                      >  4:263498/1‑261 (MQ=255)
                                                    ggCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGAc                                                                                                                                                                                                                             >  2:171047/1‑274 (MQ=255)
                                                                             aggctcCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGTTAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaa                                                                                                                                                                                                   <  2:694244/272‑1 (MQ=255)
                                                                               tctcCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCTCATCCGGCGTGAGGTGATTGCCACCCATCCACGGCCTGCCAGAGACCGGCGAAAAGTTTCCATGGCAACTaaaa                                                                                                                                                                                                 >  4:425262/1‑275 (MQ=255)
                                                                                       aCAGGTTGCACCATTTTAGTGCATTATAGCTTTTAAAGGGTAAAACTAGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCATGGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCTTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa                                                                                                                                                                                         <  4:118623/275‑1 (MQ=255)
                                                                                                             aTTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCAcc                                                                                                                                                                                                                                                  <  2:548641/196‑1 (MQ=255)
                                                                                                                ataGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTAc                                                                                                                                                                <  2:425266/275‑1 (MQ=255)
                                                                                                                           cGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAt                                                                                                                                                                                                                                                                                   <  7:1251715/149‑1 (MQ=255)
                                                                                                                               tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                                                                                                                                                               >  5:778851/1‑149 (MQ=255)
                                                                                                                               tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                                                                                                                                                               >  6:1540071/1‑149 (MQ=255)
                                                                                                                               tAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGc                                                                                                                                                                                                                                                                               >  7:79036/1‑149 (MQ=255)
                                                                                                                                  aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                                                                                                                                                            <  5:844050/149‑1 (MQ=255)
                                                                                                                                  aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                                                                                                                                                            <  7:457715/149‑1 (MQ=255)
                                                                                                                                  aaaGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTc                                                                                                                                                                                                                                                                            <  6:714366/149‑1 (MQ=255)
                                                                                                                                      cgtgAATCATCAAAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg                                                                                                                                          <  4:178400/272‑1 (MQ=255)
                                                                                                                                      cgcgAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttat                                                                                                                                                                                                                                                                        <  6:489661/149‑5 (MQ=255)
                                                                                                                                       gcgAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttatc                                                                                                                                                                                                                                                                       <  8:536249/149‑6 (MQ=255)
                                                                                                                                       gcgAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttatc                                                                                                                                                                                                                                                                       <  8:606400/149‑6 (MQ=255)
                                                                                                                                         gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                                                                                                                                                     >  8:290029/1‑149 (MQ=255)
                                                                                                                                         gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAAATGCGTctta                                                                                                                                                                                                                                                                         >  8:462297/1‑142 (MQ=255)
                                                                                                                                         gAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGAAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCg                                                                                                                                                                                                                                                                     >  7:1144403/1‑149 (MQ=255)
                                                                                                                                          aaGCCTCAGAGAATTGACGGAGAAAAAAGCCCATGCAGATATGGGCTCCAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGATCCGGCCCACCACATCATCACCGTGTAGGCCGGATGTGACGCAAATGCGTCTTATCCgg                                                                                                                                                                                                                                                                    <  8:1420002/149‑1 (MQ=255)
                                                                                                                                                cAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                                                                                                                                                              <  6:227093/149‑1 (MQ=255)
                                                                                                                                                 agagAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtg                                                                                                                                                                                                                                                             >  6:530901/1‑149 (MQ=255)
                                                                                                                                                  gagaATTGACGGAGAAAAAAGCCTATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCAttt                                                                                                                                                                                                                                                                                 >  6:838967/1‑128 (MQ=255)
                                                                                                                                                  gagaATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAtgt                                                                                                                                                                                                                                                            >  7:556185/1‑149 (MQ=255)
                                                                                                                                                       ttGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCaa                                                                                                                                                                                                       >  2:150877/1‑197 (MQ=255)
                                                                                                                                                          aCGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACg                                                                                                                                                                                                           <  3:233387/190‑1 (MQ=255)
                                                                                                                                                           cGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaa                                                                                                                                                                                                   <  4:150875/197‑1 (MQ=255)
                                                                                                                                                             gagaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCg                                                                                                                                                                                                                                                 >  8:1667874/1‑149 (MQ=255)
                                                                                                                                                               gaAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGAt                                                                                                                                                                                                                                               >  8:1504211/1‑149 (MQ=255)
                                                                                                                                                                 aaaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATcc                                                                                                                                                                                                                                             <  7:128951/149‑1 (MQ=255)
                                                                                                                                                                  aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGAt                                                                                                                                                                                                                                                              >  6:99769/1‑131 (MQ=255)
                                                                                                                                                                  aaaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATccc                                                                                                                                                                                                                                            >  7:1303306/1‑149 (MQ=255)
                                                                                                                                                                   aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                                                                                                                                                           <  7:888498/149‑1 (MQ=255)
                                                                                                                                                                   aaaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                                                                                                                                                           <  8:1167465/149‑1 (MQ=255)
                                                                                                                                                                    aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                                                                                                                                                          <  5:610992/149‑1 (MQ=255)
                                                                                                                                                                    aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                                                                                                                                                          <  8:720072/149‑1 (MQ=255)
                                                                                                                                                                    aaGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                                                                                                                                                          >  8:728170/1‑149 (MQ=255)
                                                                                                                                                                     aGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAgg                                                                                                                                                                                                                                         <  7:290029/149‑1 (MQ=255)
                                                                                                                                                                      gCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTCTTATCCGGcc                                                                                                                                                                                                                                                                  >  8:631599/1‑122 (MQ=38)
                                                                                                                                                                         cATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTg                                                                                                                                                                                                                                     >  7:57989/1‑149 (MQ=255)
                                                                                                                                                                          aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                                                                                                                                                                    >  7:421545/1‑149 (MQ=255)
                                                                                                                                                                          aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGc                                                                                                                                                                                                                                    <  8:75548/149‑1 (MQ=255)
                                                                                                                                                                          aTGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCg                                                                                                                                                        >  1:239992/1‑225 (MQ=255)
                                                                                                                                                                            gCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATGCGACGCAAATGCGTcttat                                                                                                                                                                                                                                                                        <  8:1294574/111‑5 (MQ=255)
                                                                                                                                                                              agagATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCaga                                                                                                                                                                                                                                >  7:587009/1‑149 (MQ=255)
                                                                                                                                                                                agaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAgaga                                                                                                                                                                                                                              <  5:497292/149‑1 (MQ=255)
                                                                                                                                                                                 gaTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGAc                                                                                                                                                                                                                             <  5:530901/149‑1 (MQ=255)
                                                                                                                                                                                  aTGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACa                                                                                                                                                                                                                            <  5:1052693/149‑1 (MQ=255)
                                                                                                                                                                                     ggCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTg                                                                                                                                                                                                                                                       >  1:10941/1‑119 (MQ=255)
                                                                                                                                                                                       cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa                                                                                                                                                                                                                       <  6:605111/149‑1 (MQ=255)
                                                                                                                                                                                       cTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGa                                                                                                                                                                                                                       >  5:1081344/1‑149 (MQ=255)
                                                                                                                                                                                         aCAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaa                                                                                                                                                                                                                     <  5:1218880/149‑1 (MQ=255)
                                                                                                                                                                                               aGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAACCACTTAGACGCTGTAGTACAGCCCACACTCTACCGGATGCGGACTCATAACCCCGCGGTCATCTTCTTCGCCCACCAGAGCCATGTACGCATCAAATGCTTCgccgcgtaa                                                                                  >  4:633881/1‑266 (MQ=255)
                                                                                                                                                                                                gCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAac                                                                                 >  2:321099/1‑274 (MQ=255)
                                                                                                                                                                                                        aCTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAAc                                                                                                                                                                                                      <  8:859263/149‑1 (MQ=255)
                                                                                                                                                                                                             aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                                                                                                                                                 <  8:1020326/149‑1 (MQ=255)
                                                                                                                                                                                                             aCGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaaaa                                                                                                                                                                                                 <  7:1667874/149‑1 (MQ=255)
                                                                                                                                                                                                                ttGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                                                                                                                                                    <  6:1014394/127‑1 (MQ=255)
                                                                                                                                                                                                                ttGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAacac                                                                                                                                                                                              <  5:430335/149‑1 (MQ=255)
                                                                                                                                                                                                                     gagCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTc                                                                                                                                                                                                               >  7:1537326/1‑127 (MQ=255)
                                                                                                                                                                                                                       gCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCCTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTcg                                                         <  2:136919/275‑1 (MQ=255)
                                                                                                                                                                                                                        cGACGCACCGGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTcgc                                                        <  2:2204/275‑1 (MQ=255)
                                                                                                                                                                                                                            tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                                                                                                                                                  >  8:1541004/1‑149 (MQ=255)
                                                                                                                                                                                                                            tCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                                                                                                                                                  >  5:1004998/1‑149 (MQ=255)
                                                                                                                                                                                                                              aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                                                                                                                                                           <  5:206789/106‑1 (MQ=255)
                                                                                                                                                                                                                              aCCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgtagt                                                                                                                                                                                >  5:1402362/1‑149 (MQ=255)
                                                                                                                                                                                                                               ccTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAg                                                                                                                                                                                                                                          >  7:140420/1‑90 (MQ=255)
                                                                                                                                                                                                                                cTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCAAGGCAACTAAAAAACTTAGACGCTGTAGTAc                                                                                                                                                                              >  5:1291062/1‑149 (MQ=255)
                                                                                                                                                                                                                                 tGCTCCGGCCTACCACATCATGCCCGTGTGGGCCGGATAAGGCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGCGACAGGCGAAACGTTTCCACGGCAACTAAAACGCTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCa                                                                                                                    <  2:400907/206‑1 (MQ=255)
                                                                                                                                                                                                                                 tGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCa                                                                                                                                                                                                                                           >  8:1143215/1‑87 (MQ=255)
                                                                                                                                                                                                                                    tCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGGAGTACAGCt                                                                                                                                                                          >  7:364406/1‑149 (MQ=255)
                                                                                                                                                                                                                                           tACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAActc                                                                                                                                                                   >  7:1189764/1‑149 (MQ=255)
                                                                                                                                                                                                                                           tACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAActc                                                                                                                                                                   >  6:1047169/1‑149 (MQ=255)
                                                                                                                                                                                                                                             ccACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                                                                                                                                                    <  5:750157/98‑1 (MQ=255)
                                                                                                                                                                                                                                             ccACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGaaaa                                                                                                                                                                                                                    >  6:750157/1‑98 (MQ=255)
                                                                                                                                                                                                                                                catcatCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAg                                                                                                                                                                                                                           <  6:1693504/88‑1 (MQ=255)
                                                                                                                                                                                                                                                 atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCgg                                                                                                                                                             >  5:1614521/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 atcatcACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCgg                                                                                                                                                             >  6:1273676/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     tcaCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGc                                                                                                                                                         >  8:1385976/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      caCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCg                                                                                                                                                        >  6:1171507/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      caCCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGGACAGCTCAAACTCTACCGGATGCg                                                                                                                                                        >  6:1119896/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        ccGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGa                                                                                                                                                      <  5:684892/149‑1 (MQ=255)
                                                                                                                                                                                                                                                         cGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAg                                                                                                                                                     <  7:728170/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGa                                                                                                                                                                                         <  1:60054/111‑1 (MQ=255)
                                                                                                                                                                                                                                                           tgtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTc                                                                                                                                                   <  8:1181876/149‑1 (MQ=255)
                                                                                                                                                                                                                                                            gtAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCagag                    <  2:505726/275‑1 (MQ=255)
                                                                                                                                                                                                                                                             tAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCagaga                   <  2:343368/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ccGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTaa                                                                                                                                                                                                   <  4:508990/95‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATacgcacg                                                                                                                                          >  6:324420/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      tAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCAcgcg                                                                                                                                        <  7:634284/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTc                                                                                                                                     >  8:542713/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                          aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCa                                                                                                                                    >  8:373125/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                          aCGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCAAGCGGTCa                                                                                                                                    <  7:697047/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                                                                                                                                                  >  7:1082441/1‑101 (MQ=255)
                                                                                                                                                                                                                                                                            gCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTgta                                                                                                                                                                                  <  8:1082441/101‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCAtct                                                                                                                                 <  6:201911/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                 
ATCGATTAACAAAATACTGTTAATCAGCGAGTTGAGGATCTGCCCAGCATCGGGCTGCGTGCCTGTTGCCATAAAGCAGTCTCCTGAACAGGTTGCACCATTTTAGTGCATTATAGCTTTTTACGGATAAAAAGCGCGAAGCATCAGAGAATTGACGGAGAAAAAAGCCCATGCAGAGATGGGCTACAGATAGCTGACAAACTTCACGTTGGAGAGCGACTCACCTGCTCCGGCCTACCACATCATCACCGTGTAGGCCGGATAAGACGCATTTGCGTCGCATCCGGCGTGATGTGCTTGCCACCGATCCCAGGCCTGCCAGAGACAGGCGAAAAGTTTCCACGGCAACTAAAACACTTAGACGCTGTAGTACAGCTCAAACTCTACCGGATGCGGAGTCATACGCACGCGGTCATCTTCTTCGCGACGCAGAGCGATGTACGCATCAATTGCTTCGTCAGTGAACACGCCACCGGCTTTCAGGAACTCGCGGTCCAGATCCAGTTCGTTCAGTGCTTCTTCCAGAGAGCCTGCAACCTGTGGGA  >  NC_000913/4056268‑4056812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: