Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,062,371 A→T 6.8% N42I (AAT→ATT)  yihN → MFS transporter family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,062,3710AT 6.8% 1027.7 / 46.4 295N42I (AAT→ATT) yihNMFS transporter family protein
Reads supporting (aligned to +/- strand):  ref base A (129/144);  new base T (7/13);  total (138/157)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 299 total aligned reads displayed.

AAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTC  >  NC_000913/4062117‑4062643
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                 
                                           tACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                                                                                                                                                                                                                              >  2:562072/1‑249 (MQ=255)
                                               cAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTca                                                                                                                                                                                                                                          <  4:562068/249‑1 (MQ=255)
                                                                ccATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACcg                                                                                                                                                                                           <  1:279294/279‑1 (MQ=255)
                                                                                      aTTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTAGGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAAcg                                                                                                                                                                                               >  2:102243/1‑252 (MQ=255)
                                                                                          ccTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGCAAAAGAACTGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGACAGATGCGTTTTATATCCCGATGCAGGAATATTTCCCTTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTGCTGTATATTTATTTTGCCAATAAACTACCGAACAGATACACCCTGTCATTctcact                                                                                                                                                                >  1:106071/1‑279 (MQ=255)
                                                                                                        aTTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCCCAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCCCAgt                                                                                                                                                                                     >  2:642742/1‑244 (MQ=255)
                                                                                                            tAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCATTg                                                                                                                                                                                                                                                                                 <  6:954551/149‑1 (MQ=255)
                                                                                                                 aTCGGTTAGGTGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTgg                                                                                                                                            <  1:15165/276‑1 (MQ=255)
                                                                                                                   cGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCATTGGTCAAAt                                                                                                                                                                                                                                                                          <  6:489112/149‑1 (MQ=255)
                                                                                                                    gCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCATTGGTCAAAtg                                                                                                                                                                                                                                                                         <  8:1192015/149‑2 (MQ=255)
                                                                                                                     cTTAGGGGATGGTATGCTCACGAAAAAGAGATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTg                                                                                                                                                                                                                                                                        >  7:986507/1‑149 (MQ=255)
                                                                                                                      ttAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCATTGGTCAAAtgga                                                                                                                                                                                                                                                                       <  8:226558/149‑4 (MQ=255)
                                                                                                                      ttAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTgg                                                                                                                                                                                                                                                                       >  5:891524/1‑149 (MQ=255)
                                                                                                                       tAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCATTGGTCAAAtggaa                                                                                                                                                                                                                                                                      <  6:1154131/149‑5 (MQ=255)
                                                                                                                        aGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTa                                                                                                                                                                                                                                                                     <  7:1114756/149‑1 (MQ=255)
                                                                                                                         ggggATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                                                                                                                                                                                                                                    <  8:151562/149‑1 (MQ=255)
                                                                                                                         ggggATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTaa                                                                                                                                                                                                                                                                    >  8:1120308/1‑149 (MQ=255)
                                                                                                                          gggATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAAt                                                                                                                                                                                                                                                                   >  8:1209834/1‑149 (MQ=255)
                                                                                                                          gggATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAAt                                                                                                                                                                                                                                                                   >  7:947867/1‑149 (MQ=255)
                                                                                                                               gTTATGCTCACGAAAAAGACCTGGGCGGTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACAAATGGTCAAATTNGTAATGCTATGTCGGTAAACTCATTTGTAACCTCAGTGGGCTTTTTTAGGACTATTTATTTTGCCTACAAACTACCGCCCNGATTAAACATTACATTCGCCTCCCANTCGACAAGATTAATGGGTGGTttttgtaagaca                                                                                                                           >  3:154983/1‑271 (MQ=255)
                                                                                                                               gTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTAt                                                                                                                                                                                                                                                              <  6:1185274/149‑1 (MQ=255)
                                                                                                                                 tATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGt                                                                                                                                                                                                                                                            <  8:155424/149‑1 (MQ=255)
                                                                                                                                 tATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGt                                                                                                                                                                                                                                                            <  7:1638801/149‑1 (MQ=255)
                                                                                                                                      tCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTa                                                                                                                                                                                                                                                       >  5:369888/1‑149 (MQ=255)
                                                                                                                                       aaagAAAAAGAAATTGGCGTTATTTAGTCTANNAACACTGTTTGGCGGTACAATTTATAAATTNCCGTCACTTAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCNATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCg                                                                                                                     <  3:126064/274‑1 (MQ=255)
                                                                                                                                        aCGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTaaa                                                                                                                                                                                                                                                     >  8:362393/1‑149 (MQ=255)
                                                                                                                                            aaaaGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCa                                                                                                                                                                                                                                                 <  8:701021/149‑1 (MQ=255)
                                                                                                                                             aaaGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAAt                                                                                                                                                                                                                                                                                  <  7:220906/115‑1 (MQ=255)
                                                                                                                                              aaGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAtt                                                                                                                                                                                                                                               <  7:1209834/149‑1 (MQ=255)
                                                                                                                                               aGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                                                                                                                                                                                                                              <  6:1507766/149‑1 (MQ=255)
                                                                                                                                                    tGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGt                                                                                                                                                                                                                                                            <  8:1378345/130‑1 (MQ=255)
                                                                                                                                                      ggCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTcacca                                                                                                                                                                                                                                       >  8:1656259/1‑149 (MQ=255)
                                                                                                                                                            aTTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTgg                                                                                                                                                                                                                                 <  6:831101/149‑1 (MQ=255)
                                                                                                                                                             tttAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTggg                                                                                                                                                                                                                                >  7:318426/1‑149 (MQ=255)
                                                                                                                                                                     aTTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAt                                                                                                                                                                                                                                                >  5:217385/1‑125 (MQ=255)
                                                                                                                                                                     aTTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAt                                                                                                                                                                                                                                                <  6:217385/125‑1 (MQ=255)
                                                                                                                                                                     aTTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCttttt                                                                                                                                                                                                                          >  4:171290/1‑147 (MQ=255)
                                                                                                                                                                      ttAACCCTGTGTGGCGTTACAAATTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGgc                                                                                        <  4:377025/275‑1 (MQ=255)
                                                                                                                                                                      ttAACACTGTGTGGCGTTACAATNTATAAANTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTAtttt                                                                                                                                                                                                          <  3:42233/162‑1 (MQ=255)
                                                                                                                                                                      ttAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                                                                                                                                                                                                                       <  6:730553/149‑1 (MQ=255)
                                                                                                                                                                      ttAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                                                                                                                                                                                                                       <  8:671017/149‑1 (MQ=255)
                                                                                                                                                                      ttAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                                                                                                                                                                                                                       <  8:438422/149‑1 (MQ=255)
                                                                                                                                                                      ttAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                                                                                                                                                                                                                       <  8:984771/149‑1 (MQ=255)
                                                                                                                                                                       tAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                                                                                                                                                                                                                      <  5:699901/149‑1 (MQ=255)
                                                                                                                                                                       tAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACcg                                                                                                                                                                                           <  1:182752/176‑1 (MQ=255)
                                                                                                                                                                        aaCACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGt                                                                                                                                                                                                                     <  6:891524/149‑1 (MQ=255)
                                                                                                                                                                         acacTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTg                                                                                                                                                                                                                      <  2:171293/147‑1 (MQ=255)
                                                                                                                                                                           acTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCtttt                                                                                                                                                                                                                           <  2:272598/140‑1 (MQ=255)
                                                                                                                                                                           acTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGa                                                                                                                                                                                     >  2:768659/1‑178 (MQ=255)
                                                                                                                                                                              gtgtgGCGGTACAATTTATAAATTACCGTCGCTGAAAGTAGCGTTTTATACCCCGATGCAGGAATCTTTCCATTTGACCACTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTCCTGTCTATATATTTTGCAGATAAACTACCGCTCAGATACAcc                                                                                                                                                                               >  4:606541/1‑181 (MQ=255)
                                                                                                                                                                              gtgtgGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGACAAATTg                                                                                                                                                                                                                                                                        <  1:134666/92‑1 (MQ=255)
                                                                                                                                                                               tgtgGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCTTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATaca                                                                                                                                                                                 <  4:768653/178‑1 (MQ=255)
                                                                                                                                                                               tgtgGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCtattta                                                                                                                                                                                                              >  8:59136/1‑149 (MQ=255)
                                                                                                                                                                                  ggCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCg                                                                                                                     <  2:205368/234‑1 (MQ=255)
                                                                                                                                                                                   gCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTAtttt                                                                                                                                                                                                          <  7:683020/149‑1 (MQ=255)
                                                                                                                                                                                    cGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCAt                                                                                                                                                                             <  2:473699/177‑1 (MQ=255)
                                                                                                                                                                                     ggTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGc                                                                                                                                                                                                        <  6:1691830/149‑1 (MQ=255)
                                                                                                                                                                                     ggTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGc                                                                                                                                                                                                        <  6:1570346/149‑1 (MQ=255)
                                                                                                                                                                                      gTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTCTCTGTCTATTTATTTTGcc                                                                                                                                                                                                       <  8:1431952/149‑1 (MQ=255)
                                                                                                                                                                                       tACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTTTcacc                                                                                                                                                                                                                                        <  5:1557933/115‑1 (MQ=255)
                                                                                                                                                                                       tACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCg                                                                                                                                                                                                      >  5:1251544/1‑149 (MQ=255)
                                                                                                                                                                                        aCAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTGTGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGa                                                                                                                                                                                                     <  5:1472458/149‑1 (MQ=255)
                                                                                                                                                                                         cAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                                                                                                                                                                                                                              <  5:1287072/107‑1 (MQ=255)
                                                                                                                                                                                         cAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                                                                                                                                                                                                                              >  6:1287072/1‑107 (MQ=255)
                                                                                                                                                                                         cAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTTCCGATAAANTACCNCGCAGATACACCATGTCATTCTCACTCATTGc                                                                                                                                                          >  4:716344/1‑190 (MQ=255)
                                                                                                                                                                                          aaTTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATa                                                                                                                                                                                                   >  7:1594406/1‑149 (MQ=255)
                                                                                                                                                                                          aaTTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATa                                                                                                                                                                                                   >  7:710503/1‑149 (MQ=255)
                                                                                                                                                                                            tttATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATaaa                                                                                                                                                                                                 <  7:1375873/149‑1 (MQ=255)
                                                                                                                                                                                             ttATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACa                                                                                                                                                      <  2:716349/190‑1 (MQ=255)
                                                                                                                                                                                                 aaaTTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCg                                                                                                                                                                                                      <  5:928826/139‑1 (MQ=255)
                                                                                                                                                                                                   aTTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCAttt                                                                                                                                                                                                                                              <  6:1319554/97‑1 (MQ=255)
                                                                                                                                                                                                           cGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATac                                                                                                                                                                                  <  8:12997/149‑1 (MQ=255)
                                                                                                                                                                                                             cTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTg                                             >  1:103848/1‑279 (MQ=255)
                                                                                                                                                                                                                   gATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTc                                                                                                                                                                          <  7:457231/149‑1 (MQ=255)
                                                                                                                                                                                                                    aTGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCa                                                                                                                                                                         >  6:254546/1‑149 (MQ=255)
                                                                                                                                                                                                                         ttttATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATtctc                                                                                                                                                                   >  7:1056539/1‑149 (MQ=255)
                                                                                                                                                                                                                         ttttATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGAGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATtctc                                                                                                                                                                   >  6:1637789/1‑149 (MQ=255)
                                                                                                                                                                                                                                tCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCAtt                                                                                                                                                            <  7:587647/149‑1 (MQ=255)
                                                                                                                                                                                                                                  ccGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGc                                                                                                                                                          >  5:316519/1‑149 (MQ=255)
                                                                                                                                                                                                                                  ccGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGc                                                                                                                                                          >  7:1444986/1‑149 (MQ=255)
                                                                                                                                                                                                                                   cGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTAc                                                                                                                                                                                             >  2:614364/1‑114 (MQ=255)
                                                                                                                                                                                                                                    gATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGa                                                                                                                                                        <  6:1247596/149‑1 (MQ=255)
                                                                                                                                                                                                                                        cAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAgg                                                                                                                                                    >  6:1442463/1‑149 (MQ=255)
                                                                                                                                                                                                                                         aGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCt                                                                                                                                                                                                                       >  7:1143469/1‑82 (MQ=255)
                                                                                                                                                                                                                                            aaTATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAAc                                                                                                                                                                                                <  6:562224/102‑1 (MQ=255)
                                                                                                                                                                                                                                            aaTATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAAc                                                                                                                                                                                                >  5:562224/1‑102 (MQ=255)
                                                                                                                                                                                                                                             atatTTCCATTTGACCAATGGTTAAAATGGTAATGATATGTCGGGAAACTCATTTGTCACCACAGTGTGCTTTTTTCTGTGTATTTCTTTTGCCGCTAAACTACCGCGCAGCTACACCTTGTCATTCTTCCTCATGGCGACAGGTTTACTTGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATa                 <  4:274332/275‑1 (MQ=255)
                                                                                                                                                                                                                                              attaccaaTTTGACCATTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACt                                                                                                                                              >  7:153688/8‑149 (MQ=255)
                                                                                                                                                                                                                                                tttCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCtt                                                                                                 >  4:621306/1‑192 (MQ=255)
                                                                                                                                                                                                                                                    aaTTTGACCATTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGa                                                                                                                                                   <  7:1190829/137‑1 (MQ=255)
                                                                                                                                                                                                                                                     aTTTGACCATTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGtt                                                                                                                                       >  7:771208/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aTTTGACCATTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGtt                                                                                                                                       >  7:53028/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aTTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGtt                                                                                                                                       >  7:778875/1‑149 (MQ=255)
                                                                                                                                                                                                                                                       ttGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACa                                                                                                                           >  4:495172/1‑159 (MQ=255)
                                                                                                                                                                                                                                                         gACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTAtt                                                                                                                                   <  8:466471/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTg                                                                                                                                 >  5:732877/1‑149 (MQ=255)
                                                                                                                                                                                                                                                           ccAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGc                                                                                                                       <  2:495176/159‑1 (MQ=255)
                                                                                                                                                                                                                                                            cAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTga                                                                                                                                <  7:739935/149‑1 (MQ=255)
                                                                                                                                                                                                                                                            cAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTga                                                                                                                                >  7:366674/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             aaTGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTgac                                                                                                                               <  8:805478/149‑1 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                 
AAGAGGCAAACCATCTTCTGAACCACTAATAAGTTTTAACTCATACGCAGTTAATAAAACCATTCCATATGAATGGTCGGTATCTTATTGCCTTTAAAACAGTGATTTTAAAAATCGCTTAGGGGATGTTATGCTCACGAAAAAGAAATGGGCGTTATTTAGTCTATTAACACTGTGTGGCGGTACAATTTATAAATTACCGTCGCTGAAAGATGCGTTTTATATCCCGATGCAGGAATATTTCCATTTGACCAATGGTCAAATTGGTAATGCTATGTCGGTAAACTCATTTGTCACCACAGTGGGCTTTTTTCTGTCTATTTATTTTGCCGATAAACTACCGCGCAGATACACCATGTCATTCTCACTCATTGCGACAGGATTACTGGGTGTTTATTTGACGACAATGCCGGGGTATTGGGGCATCCTCTTTGTCTGGGCGCTATTTGGCGTTACTTGCGACATGATGAACTGGCCGGTCTTGCTCAAGTCGGTAAGTCGATTGGGCAATAGCGAACAACAAGGTC  >  NC_000913/4062117‑4062643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: