| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 657,531 | T→G | 8.0% | intergenic (+30/+24) | cspE → / ← flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 657,531 | 0 | T | G | 8.0% | 972.5 / 61.2 | 302 | intergenic (+30/+24) | cspE/flc | constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (150/126); new base G (11/15); total (161/141) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.13e-02 | |||||||||||
Only 100 of 306 total aligned reads displayed.
TCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATG > NC_000913/657265‑657805 | tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGTTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg > 2:409448/1‑273 (MQ=255)tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg > 2:652159/1‑273 (MQ=255) aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACTCTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt < 1:119673/275‑1 (MQ=255) aTTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATTGTTTTAACACTATTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGCCCCTTCAGATGCAACCGTAATCGCACTG‑AAAGATACGTCAGCAAGAAGTAAAAACCCGCTTAATCAACGGGATTTTTTTGGTCTTTAgtg > 4:390117/1‑275 (MQ=255) tttAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGc > 4:463453/1‑275 (MQ=255) gACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCGAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGTTCTTTAGTGTGCGGCTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCCAAAGACGCCAAACGGTTGACGaaactgt > 2:668613/1‑270 (MQ=255) acTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATGCAAAACCCGCTTAAt > 6:500660/1‑149 (MQ=255) ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa > 6:1141945/1‑149 (MQ=255) ttCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCa < 7:553197/149‑1 (MQ=255) tctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg > 8:1172501/1‑149 (MQ=255) tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg > 5:488093/1‑149 (MQ=255) tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCATAATGCCAGTTCGGTAATGGCAAAAGACCCCAGAAGGTTTACGAAAACGTTAAGTAATGCCCAGCCAAAGCGGACCACTTGt > 4:576542/1‑274 (MQ=255) cAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtt > 5:54142/1‑149 (MQ=255) aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt < 7:1586281/149‑1 (MQ=255) aTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttt > 5:1209541/1‑149 (MQ=255) tCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGttttt > 8:1230871/1‑149 (MQ=255) cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa < 7:1103667/149‑4 (MQ=255) gACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg > 5:393934/1‑149 (MQ=255) tAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTc > 8:797839/1‑149 (MQ=255) aaTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt < 5:1371849/149‑1 (MQ=255) ggTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCtt < 5:1301813/147‑1 (MQ=255) ttttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt > 7:1317415/1‑149 (MQ=255) tttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTg > 5:1639046/1‑139 (MQ=255) tttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtg > 6:36867/1‑149 (MQ=255) aaaaCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGc > 6:1111265/1‑149 (MQ=255) aCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt > 5:1397103/1‑142 (MQ=255) ctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa < 6:1297687/149‑1 (MQ=255) ctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGt > 2:192651/1‑275 (MQ=255) tGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg < 7:1250753/149‑1 (MQ=255) tCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGaa > 2:101467/1‑145 (MQ=255) aGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGc < 8:15104/149‑1 (MQ=255) gcgcGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc > 5:1044488/1‑149 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACa < 4:101466/145‑1 (MQ=255) cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCa < 5:1607038/149‑1 (MQ=255) gTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCa < 5:1126710/149‑1 (MQ=255) ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAAt < 5:995202/144‑1 (MQ=255) ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg > 5:1673655/1‑149 (MQ=255) ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAg > 5:770158/1‑149 (MQ=255) tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGt < 6:1209541/149‑1 (MQ=255) tCGAAATCACTAACGGGGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCcaca < 2:390121/274‑1 (MQ=255) atcccaaaCTGTCCCAAAGTCCCTTCTGCTGCANGGTTACTCGCTCTG‑TAATATACGTCNGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCNAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCggtg < 3:180740/273‑1 (MQ=255) tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc < 7:764222/132‑1 (MQ=255) cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCg < 2:343408/274‑1 (MQ=255) cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCCAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCAAGTGCGGTCATGGCAAAAGACCCCAGCAGGTTGACGAAAACGTTCTGTAATTCCAAGCCAAAGCGGCCCTCTTTTAACAAAAACACCACTTCTACCGAAACTGTTGNNNAGG‑TGGTTAGACCACCCAAAAAACCtgtggtt > 3:266243/1‑277 (MQ=255) tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa > 6:461190/1‑114 (MQ=255) tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa < 5:461190/114‑1 (MQ=255) tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATgg < 6:298303/149‑1 (MQ=255) aCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCa > 6:437242/1‑149 (MQ=255) ggTGCCAAAGGCCCTTCTTCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt < 1:266243/279‑1 (MQ=255) ggTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGAt < 2:575074/275‑1 (MQ=255) gTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa < 6:121731/149‑1 (MQ=255) ccAAAGGCCGTTCTGCTGGAAACG‑AATTGTACTGTTAACATACGGAATCAAGAATTCAAAAACGGGTGAAGGAGCGGGTTTTTTTTGGTCTTTAGTGTGGCGTAGAGGCCGAAAAAAGCCAGAAGGCAAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAGGAAAACGTTCAGCAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAAACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt < 4:296738/275‑1 (MQ=255) ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAc > 7:1478921/1‑149 (MQ=255) ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCANGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTTTACCAAAAACACCACTTNAGCCGAAAATGTTTAGAAGGTTTGGNAAACC‑CCAAAAAACCAGGTGGTTATTAATACt > 3:158485/1‑278 (MQ=255) ccAAAGGCCCTTCGGCTCCAACCGTTCACTGTCGG‑AAGGATACGTCAGCAAGAATTCAAAACCAGCTTAATCGGCGGGGTTTTTTTGGTGTTTAGTGTGAGGTGGAGGCCGAAAACAGACAGAAGGCCAGTGCTGTAATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTTAGTGATACCCAGCCTAAGCGGGCAATAAATAACAAAAACGCCACTTCAGACGAAAATAGTGAGAAGA‑TTGTTATACCGCCACAAAATCCGGTGGTGATTaag < 4:62671/275‑2 (MQ=255) gacaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt < 2:256101/271‑1 (MQ=255) cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcc < 5:817159/149‑1 (MQ=255) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt < 8:416433/79‑1 (MQ=38) aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt > 7:416433/1‑79 (MQ=38) aaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc > 7:645832/1‑149 (MQ=255) aaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc > 5:573784/1‑149 (MQ=255) gCCCTTCTGCTGACAACGTAAGCCGTCTG‑TAAAGAACGGAAGAATGAGTTCAAAACCCGCGTAATCAGCGGGTTTTATTTGGTGTTTAGTGGGCGGTTGAGGCCGAAAACAGCGAGAATGCCAGTGCGGTCATGGGAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAGGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc < 4:192648/275‑1 (MQ=255) cccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa < 8:1006598/149‑1 (MQ=255) cccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGaa < 7:797839/149‑1 (MQ=255) ccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGcc > 4:659864/1‑112 (MQ=255) ccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa > 5:671358/1‑138 (MQ=255) ccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa < 6:671358/138‑1 (MQ=255) cTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAgg > 6:1332082/1‑149 (MQ=255) ttCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg < 1:17367/160‑1 (MQ=255) gctgcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGCGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt < 2:502477/146‑1 (MQ=255) gctgcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc < 5:314671/149‑1 (MQ=255) gcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTTCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGCAAATTTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGGGCTTAATTCTTTCAacacctgtct > 4:256098/1‑269 (MQ=255) gcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa > 7:1407210/1‑129 (MQ=255) gcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCaaaa < 8:1407210/129‑1 (MQ=255) gTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt > 7:967528/1‑149 (MQ=255) gaaTCACTCTG‑TAAGATACGTAAGTAAGAATTCAAAATCCGCTGAATCAGCGGGTTGTTTTTGGGCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGAGGTCATGGCAAAAGACCCCAGAAGGTGGAAGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCa < 1:201062/278‑1 (MQ=255) aTCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTTAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAg > 6:1576513/1‑149 (MQ=255) ctctG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTc < 8:359346/107‑1 (MQ=255) tctG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGt < 5:432384/58‑1 (MQ=255) tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCg > 4:520272/1‑99 (MQ=255) tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt < 7:1002012/137‑1 (MQ=255) gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt > 8:267094/1‑117 (MQ=255) aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTg < 7:268235/83‑1 (MQ=255) aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct > 7:1657736/1‑149 (MQ=255) aaGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCct > 6:1636774/1‑149 (MQ=255) aGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTGTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtccta < 1:281432/277‑1 (MQ=255) aaTTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGATGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCtt > 8:132489/1‑149 (MQ=255) aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc > 6:756061/5‑133 (MQ=255) ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGTCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCgtgt > 6:848627/1‑149 (MQ=255) tCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTa > 8:1666569/1‑149 (MQ=255) aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc < 6:1419945/94‑1 (MQ=255) aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc > 6:1536584/1‑149 (MQ=255) aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc > 5:909919/1‑149 (MQ=255) aaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCAAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattccta < 2:613030/274‑1 (MQ=255) ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaaa > 8:584985/1‑149 (MQ=255) ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTTGACTGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTc > 2:287384/1‑237 (MQ=255) gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGtt < 8:67515/40‑1 (MQ=255) cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGc > 4:325612/1‑77 (MQ=255) cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc > 6:1657745/1‑98 (MQ=255) cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAacac < 5:1497406/149‑1 (MQ=255) | TCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAACAGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATG > NC_000913/657265‑657805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |