Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 657,535 C→A 7.7% intergenic (+34/+20) cspE → / ← flc constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913657,5350CA 7.7% 1034.0 / 54.9 308intergenic (+34/+20)cspE/flcconstitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein/fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand):  ref base C (151/130);  new base A (10/14);  total (162/146)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-04

Only 100 of 308 total aligned reads displayed.

TCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAAT  >  NC_000913/657265‑657812
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                       
tCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGTTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAg                                                                                                                                                                                                                                                                                       >  2:409448/1‑273 (MQ=255)
   aTTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACTCTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGt                                                                                                                                                                                                                                                                                  <  1:119673/275‑1 (MQ=255)
                                              aCGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGGTGAGGCCGAAAAcctgtctctt                                                                                                                                                                                                                                    >  1:99449/1‑269 (MQ=255)
                                                    aGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCACAGGCCCTTCTGCTGCAAACGTCATCGCTCTG‑TAAGATCCGTCAGCAAGAATTCAAAACCCGCTTCATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTTAGGCCGAAAAC‑AGCCAGAATGCCAGTg                                                                                                                                                                                                                            >  1:280875/1‑279 (MQ=255)
                                                                  tCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGc                                                                                                                                                                                                                  >  4:644390/1‑275 (MQ=255)
                                                                      aaGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGcc                                                                                                                                                                                                                                                   <  2:654740/239‑1 (MQ=255)
                                                                       aGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTATTGTGCGGTTGAGGCCGAAAAC‑AGCCACAATGCCAGTGCGGGCATGGCAAAAg                                                                                                                                                                                                             >  2:173725/1‑275 (MQ=255)
                                                                                                                             ctctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGc                                                                                                                                                                                                                                                                                      <  5:459349/149‑1 (MQ=255)
                                                                                                                               ctGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCgg                                                                                                                                                                                                                                                                                    <  7:86873/149‑1 (MQ=255)
                                                                                                                                tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                                                                                                                                                                                                                   >  5:1075637/1‑149 (MQ=255)
                                                                                                                                tGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCggg                                                                                                                                                                                                                                                                                   >  7:15104/1‑149 (MQ=255)
                                                                                                                                 gCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCg                                                                                                                                                                                                                                                                                     <  5:1609347/146‑1 (MQ=255)
                                                                                                                                   aaTCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                                                                                                                                                                                                                <  7:1600098/149‑1 (MQ=255)
                                                                                                                                     tCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTTTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaa                                                                                                                                                >  2:62671/1‑274 (MQ=255)
                                                                                                                                      ccAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttga                                                                                                                                                                                                                                                                             <  6:462114/149‑3 (MQ=255)
                                                                                                                                       cAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                                                                                                                                                                                                            <  7:1103667/149‑4 (MQ=255)
                                                                                                                                        aGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTGATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGtttttttt                                                                                                                                                                                                                                                                           <  7:975197/149‑1 (MQ=255)
                                                                                                                                          aCTTATGGTTTTAAAACTCTTGCTGAATTTCAGCGCGTAGCGTCCGAATTCACTAACGGTGCCAACGGGCCTTCTGATGCAAACGTAATCGCTCTT‑TAAGCTACGTCAGCTAGAATTCATAACCCTTTTAAGCAGCGGGCTTTTCTTGCTCTTTAGTCTGCGTTTGAGGCCGAAAAC‑ACCCAGAATGCCAGTCCGTGCATGGCACAAGACCCCAGAAGGTTGACGAAAACGTTAAGTAAGGTCCAGCCAACGCGGCCCCCTTATACCaacacaac                                                                                                                                          >  4:465321/1‑269 (MQ=255)
                                                                                                                                          aCTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTgg                                                                                                                                                                                                                                                                         <  8:392070/149‑1 (MQ=255)
                                                                                                                                             aaTGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCt                                                                                                                                                                                                                                                                      <  5:1371849/149‑1 (MQ=255)
                                                                                                                                               tGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGACTGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACtt                                                                                                                                     >  2:216898/1‑275 (MQ=255)
                                                                                                                                                ggTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCtt                                                                                                                                                                                                                                                                     <  5:1301813/147‑1 (MQ=255)
                                                                                                                                                ggTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCtt                                                                                                                                                                                                                                                                     <  5:1645115/147‑1 (MQ=255)
                                                                                                                                                ggTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTa                                                                                                                                                                                                                                                                   <  5:206838/149‑1 (MQ=255)
                                                                                                                                                 gTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAg                                                                                                                                                                                                                                                                  <  5:1239881/149‑1 (MQ=255)
                                                                                                                                                   tttAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgtg                                                                                                                                                                                                                                                                >  6:36867/1‑149 (MQ=255)
                                                                                                                                                    ttAAAACTCTTGCTGAAGGTCATCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                                      <  1:191067/221‑1 (MQ=255)
                                                                                                                                                         aCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt                                                                                                                                                                                                                                                                 >  5:1397103/1‑142 (MQ=255)
                                                                                                                                                         aCTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAgt                                                                                                                                                                                                                                                                 <  6:1397103/142‑1 (MQ=255)
                                                                                                                                                            ctTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGCGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCACAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGa                                                                                                                                                                                                                                                       <  8:1375851/149‑1 (MQ=255)
                                                                                                                                                              tGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAgg                                                                                                                                                                                                                                                     <  7:1250753/149‑1 (MQ=255)
                                                                                                                                                               gCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGc                                                                                                                                                                                                                                                    <  5:154498/149‑1 (MQ=255)
                                                                                                                                                                 tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                                                                                                                                                                                                                <  7:574681/119‑1 (MQ=255)
                                                                                                                                                                 tGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttt                                                                                                                                                                                                                                                                                >  8:574681/1‑119 (MQ=255)
                                                                                                                                                                  gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGa                                                                                                                                                                                                                                                 >  6:1550104/1‑149 (MQ=255)
                                                                                                                                                                  gAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGa                                                                                                                                                                                                                                                 >  8:345586/1‑149 (MQ=255)
                                                                                                                                                                        cAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCCTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑a                                                                                                                                                                                                                                           <  5:742409/148‑1 (MQ=255)
                                                                                                                                                                           cgcgTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑a                                                                                                                                                                                                                                           <  4:101466/145‑1 (MQ=255)
                                                                                                                                                                              gTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGaa                                                                                                                                                                                                                                    <  6:1000932/149‑1 (MQ=255)
                                                                                                                                                                                    ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAg                                                                                                                                                                                                                              >  6:1617739/1‑149 (MQ=255)
                                                                                                                                                                                    ttCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGttttgaa                                                                                                                                                                                                                                                                            >  8:1467698/1‑101 (MQ=39)
                                                                                                                                                                                     tCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGt                                                                                                                                                                                                                             <  6:1124284/149‑1 (MQ=255)
                                                                                                                                                                                     tCGAAATCACTAACGGGGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCcaca                                                                                               <  2:390121/274‑1 (MQ=255)
                                                                                                                                                                                          atcccaaaCTGTCCCAAAGTCCCTTCTGCTGCANGGTTACTCGCTCTG‑TAATATACGTCNGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCNAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCggtg                                                                                     <  3:180740/273‑1 (MQ=255)
                                                                                                                                                                                           tCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTc                                                                                                                                                                                                                       >  6:1280686/1‑149 (MQ=255)
                                                                                                                                                                                            cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCg                                                                                        <  2:343408/274‑1 (MQ=255)
                                                                                                                                                                                            cACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTAAGGCCGAAAAC‑AGCCCGAATGCCAGTTCGGTCATGGCAAAAGAACCCAGAAGTTTGACGAAAACGTTAAGTAATGCCCAGCCAAAGCGGCCATCTTGTAACAAAAACACCAATTCTGCCGAAAATTTTGCTAAGG‑TTTGTAGACCGAAACAACATCCgg                                                                                       >  4:578273/1‑275 (MQ=255)
                                                                                                                                                                                             aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCAt                                                                                                                                                                                                                     <  7:473581/149‑1 (MQ=255)
                                                                                                                                                                                             aCTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGATTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCAt                                                                                                                                                                                                                     >  8:1103517/1‑149 (MQ=255)
                                                                                                                                                                                              cTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCCAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCAAGTGCGGTCATGGCAAAAGACCCCAGCAGGTTGACGAAAACGTTCTGTAATTCCAAGCCAAAGCGGCCCTCTTTTAACAAAAACACCACTTCTACCGAAACTGTTGNNNAGG‑TGGTTAGACCACCCAAAAAACCtgtggtt                                                                                  >  3:266243/1‑277 (MQ=255)
                                                                                                                                                                                               tAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATgg                                                                                                                                                                                                                   <  6:298303/149‑1 (MQ=255)
                                                                                                                                                                                                aaCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATg                                                                                                                                                                                                                    <  2:277951/147‑1 (MQ=255)
                                                                                                                                                                                                 aCGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCa                                                                                                                                                                                                                 >  6:437242/1‑149 (MQ=255)
                                                                                                                                                                                                       ccAAAGGCCGTTCTGCTGGAAACG‑AATTGTACTGTTAACATACGGAATCAAGAATTCAAAAACGGGTGAAGGAGCGGGTTTTTTTTGGTCTTTAGTGTGGCGTAGAGGCCGAAAAA‑AGCCAGAAGGCAAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAGGAAAACGTTCAGCAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAAACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAAt                                                                            <  4:296738/275‑1 (MQ=255)
                                                                                                                                                                                                       ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGa                                                                                                                                                                                                     <  1:221910/155‑1 (MQ=255)
                                                                                                                                                                                                       ccAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCANGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTTTACCAAAAACACCACTTNAGCCGAAAATGTTTAGAAGGTTTGGNAAACC‑CCAAAAAACCAGGTGGTTATTAATACt                                                                         >  3:158485/1‑278 (MQ=255)
                                                                                                                                                                                                        cAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTGATTAAGCGGGtt                                                                                                                                                                                                                                                                                 <  5:461460/79‑1 (MQ=38)
                                                                                                                                                                                                         aaaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGAccc                                                                                                                                                                                                         >  8:673863/1‑149 (MQ=255)
                                                                                                                                                                                                          aaGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                                                                                                                                                                                                        >  7:645832/1‑149 (MQ=255)
                                                                                                                                                                                                          aaGGCCCTTCTGCTGCAAACGTAATCACTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGAcccc                                                                                                                                                                                                        >  6:1208830/1‑149 (MQ=255)
                                                                                                                                                                                                           aGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGGTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                                    <  4:400163/168‑1 (MQ=255)
                                                                                                                                                                                                             gCCCTTCTGCTGACAACGTAAGCCGTCTG‑TAAAGAACGGAAGAATGAGTTCAAAACCCGCGTAATCAGCGGGTTTTATTTGGTGTTTAGTGGGCGGTTGAGGCCGAAAAC‑AGCGAGAATGCCAGTGCGGTCATGGGAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAGGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTc                                                                      <  4:192648/275‑1 (MQ=255)
                                                                                                                                                                                                               ccTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCaaaa                                                                                                                                                                                                              <  6:671358/138‑1 (MQ=255)
                                                                                                                                                                                                                 ttCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                                      <  1:17367/160‑1 (MQ=255)
                                                                                                                                                                                                                  tCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGtt                                                                                                                                                                                                <  5:1141945/149‑1 (MQ=255)
                                                                                                                                                                                                                     gctgcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc                                                                                                                                                                                             <  8:263516/149‑1 (MQ=255)
                                                                                                                                                                                                                        gcAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTTCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGCAAATTTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGGGCTTAATTCTTTCAacacctgtct                                                           >  4:256098/1‑269 (MQ=255)
                                                                                                                                                                                                                          aaaCGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGaaaa                                                                                                                                                                                        >  6:1305655/1‑149 (MQ=255)
                                                                                                                                                                                                                           aaCGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAAc                                                                                                                                                                                       <  7:244006/149‑1 (MQ=255)
                                                                                                                                                                                                                            aCGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACg                                                                                                                                                                                      >  6:121447/1‑149 (MQ=255)
                                                                                                                                                                                                                              gTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                                    >  5:511850/1‑149 (MQ=255)
                                                                                                                                                                                                                              gTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                                    <  5:334768/149‑1 (MQ=255)
                                                                                                                                                                                                                                 aTCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAg                                                                                                                                                                                 >  5:542460/1‑149 (MQ=255)
                                                                                                                                                                                                                                     ctctG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTc                                                                                                                                                                                                                       >  7:359346/1‑107 (MQ=255)
                                                                                                                                                                                                                                        tG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGcc                                                                                                                                                                          >  5:396887/1‑149 (MQ=255)
                                                                                                                                                                                                                                           tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGtt                                                                                                                                                                                    <  7:1002012/137‑1 (MQ=255)
                                                                                                                                                                                                                                           tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCa                                                                                                                                                                        <  8:774274/149‑1 (MQ=255)
                                                                                                                                                                                                                                           tAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCa                                                                                                                                                                        >  5:800189/1‑149 (MQ=255)
                                                                                                                                                                                                                                               aTACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCa                                                                                                                                                                                                                      <  2:520276/99‑1 (MQ=255)
                                                                                                                                                                                                                                                   gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCa                                                                                                                                                                                                                 >  4:731037/1‑100 (MQ=255)
                                                                                                                                                                                                                                                   gTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGc                                                                                                                                                                <  5:842121/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     cAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGAACGCCACAAAATCCGGTGGTGATTAATACTTTCCCCACTGGATCAATGTTCGTCATCCTGCTGCACCATGCg                               >  2:30204/1‑275 (MQ=255)
                                                                                                                                                                                                                                                       gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAg                                                                                                                                                                                                             <  2:731042/100‑1 (MQ=255)
                                                                                                                                                                                                                                                       gCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGcc                                                                                                                                                            <  5:930219/149‑1 (MQ=255)
                                                                                                                                                                                                                                                             aaaaaaaaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGc                                                                                                                                                                      >  6:756061/5‑133 (MQ=255)
                                                                                                                                                                                                                                                              ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGTTCTTTATTGTGCGGTTG‑GGCCCAAAACAAGCAAAATTGCAATTGGGGTAAGGCAAAAAGACCCCGGAAGGTTAAGGAAAAGTTTAAGAATTCCCCAGCAAAAGCGCCcttcttta                                                                                                                                                     >  8:1230479/1‑147 (MQ=255)
                                                                                                                                                                                                                                                              ttCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGTCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCgtgt                                                                                                                                                     >  6:848627/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                cAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTaa                                                                                                                                                   <  6:460456/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCtt                                                                                                                                                       >  8:1622756/1‑144 (MQ=255)
                                                                                                                                                                                                                                                                 aaaaCCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAAc                                                                                                                                                  >  7:1456586/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                 aaaaCCCGCTTAATAGGCGGGGTTTTTTTGGTTTTTAGGGTGGGGTGGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGAAAAAGACCCGAGAAGGTTGACGAAAACGTTCAGTAATGCACAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCGGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAAtcctattcct                   <  4:570061/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaa                                                                                                                                                >  5:645494/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCGCTGATTAAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaa                                                                                                                                                >  7:789736/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                      ccGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACaaaaa                                                                                                                                             >  6:1515332/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                        gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGtt                                                                                                                                                                                                                                                         >  7:67515/1‑40 (MQ=255)
                                                                                                                                                                                                                                                                        gCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGcc                                                                                                                                                                          >  4:330908/1‑118 (MQ=255)
                                                                                                                                                                                                                                                                         cTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGAc                                                                                                                                                                                             >  6:1657745/1‑98 (MQ=255)
                                                                                                                                                                                                                                                                           tAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGCCCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAAcacca                                                                                                                                        <  7:1361766/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATg         <  2:576546/275‑1 (MQ=255)
                                                                                                                                                                                                                                                                             aTCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATTCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACAACt                                                                                                                                      >  8:1273515/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                       
TCTATTTTTCATGTAAAGGTAATTTTGATGTCTAAGATTAAAGGTAACGTTAAGTGGTTTAATGAGTCCAAAGGATTCGGTTTCATTACTCCGGAAGACGGCAGCAAAGACGTGTTCGTACACTTCTCTGCAATCCAGACTAATGGTTTTAAAACTCTTGCTGAAGGTCAGCGCGTAGAGTTCGAAATCACTAACGGTGCCAAAGGCCCTTCTGCTGCAAACGTAATCGCTCTG‑TAAGATACGTCAGCAAGAATTCAAAACCCGCTTAATCAGCGGGTTTTTTTTGGTCTTTAGTGTGCGGTTGAGGCCGAAAAC‑AGCCAGAATGCCAGTGCGGTCATGGCAAAAGACCCCAGAAGGTTGACGAAAACGTTCAGTAATGCCCAGCCAAAGCGGCCCTCTTGTAACAAAAACACCACTTCTGCCGAAAATGTTGAGAAGG‑TTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAAT  >  NC_000913/657265‑657812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑