BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	770,805	G→A	6.7%	intergenic (+194/‑653)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	770,805	0	G	A	6.7%	566.6 / 18.2	176	intergenic (+194/‑653)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand): ref base G (78/85); new base A (5/7); total (83/93)
Fisher's exact test for biased strand distribution p-value = 7.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 176 total aligned reads displayed.

CGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGACC  >  NC_000913/770533‑770952

cagaaaaaaTCAAGTTTTAAATATTTCGTGGCTNNNTTTACCCTGCCAGTAACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                               <  3:290435/274‑1 (MQ=39)

    gAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                               <  2:60507/275‑1 (MQ=255)

    gAAAATAAAGGGTCAAAACATACTGTGCCTTTTTTTACCGGCCGGTCACGGACGTGAAGTTACCGGCTTGCCTGAGAGACAATCAAACCGAAGCCACTGAGACGCGGCCAGATTGTTTACAAAGGGCGATTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTGTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                               <  4:482564/275‑1 (MQ=255)

                      ttttctggtccTGTTTTCCCCGCCCAGTCCCGTACCTGCCGTTACCGACTGTCCTTAATATCAATCAAACCGAATCCAAATATGCGCGTCCGAATTGTTGATAAAGGGCGCTTTGTTCATGCCGGATACTGCATGATCGCGTTATTCGGTCTACAAAAGTAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGTGCATCAGGTAATTTTGCTTTTATCTTCACTCTCAAGCCACATATATGTGGCTTTATTTTTAACaa                                                                                                                             <  4:413639/266‑1 (MQ=255)

                      cTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaa                                                                                                                             <  2:362876/275‑1 (MQ=255)

                           tgggCCTTGTTTACCCGTCCAGTCCCGAACTTTGTGTTACCGGCTTGCCTGGATATGAATCAAGCCGAATCCACATATGTGCGGCCAGATTGTTGACTAAGGGCGCTTTGGTCATGCCGGTGACGTCATGAACGCGTTTGTCGGTCTACGAAAGCAGGCAAGTTCAATATAGTGCATAGATTATGTAGACACTTGCAAGCGTCGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTAGATGTGGCTGTATTTTTGACAAaagaa                                                                                                                        <  4:240028/274‑1 (MQ=255)

                                                                                                                              aGGGCGATTTGTTCATGCCGGATACGGCATGAACGCTCTATTCGGACTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGta                                                                                                                                                   >  7:716677/1‑149 (MQ=255)

                                                                                                                                 gcgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTatat                                                                                                                                                <  5:1633655/149‑1 (MQ=255)

                                                                                                                                 gcgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTatat                                                                                                                                                >  5:524580/1‑149 (MQ=255)

                                                                                                                                  cgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                               <  5:480373/149‑1 (MQ=255)

                                                                                                                                   gcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtgt                                                                                                                                              <  5:598841/149‑1 (MQ=255)

                                                                                                                                     tttGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTgg                                                                                                                                            <  8:847809/149‑1 (MQ=255)

                                                                                                                                     tttGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTgg                                                                                                                                            >  5:467521/1‑149 (MQ=255)

                                                                                                                                         ttCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCttt                                                                                                                                        <  5:474850/149‑1 (MQ=255)

                                                                                                                                         ttCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCttt                                                                                                                                        >  7:556718/1‑149 (MQ=255)

                                                                                                                                          tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                       <  8:1253440/149‑3 (MQ=255)

                                                                                                                                          tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCCTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTa                                                                                                                                       <  8:1053129/149‑1 (MQ=255)

                                                                                                                                              gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAtttt                                                                                                                                   <  6:1485375/149‑1 (MQ=255)

                                                                                                                                                 ggATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTaa                                                                                                                                <  6:467521/149‑1 (MQ=255)

                                                                                                                                                       ggCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaat                                                                                                                          >  6:116776/1‑149 (MQ=255)

                                                                                                                                                           tGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAataata                                                                                                                      >  8:1634311/1‑149 (MQ=255)

                                                                                                                                                           tGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAataata                                                                                                                      >  8:682886/1‑149 (MQ=255)

                                                                                                                                                            gAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAAtaataa                                                                                                                     <  8:1667920/149‑1 (MQ=255)

                                                                                                                                                              aCGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAAcc                                                                                                                   >  7:1639741/1‑149 (MQ=255)

                                                                                                                                                                gCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCt                                                                                                                 <  7:1520738/149‑1 (MQ=255)

                                                                                                                                                                  tttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                               >  8:208247/1‑149 (MQ=255)

                                                                                                                                                                  tttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                               >  6:747304/1‑149 (MQ=255)

                                                                                                                                                                  tttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                               <  8:421196/149‑1 (MQ=255)

                                                                                                                                                                   ttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTggg                                                                                                              >  6:1248985/1‑149 (MQ=255)

                                                                                                                                                                     aTTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttt                                                                                                                                    >  7:1655470/1‑125 (MQ=255)

                                                                                                                                                                     aTTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttt                                                                                                                                    <  8:1655470/125‑1 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                           >  6:428169/1‑149 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                           <  7:139626/149‑1 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                       >  5:889113/1‑119 (MQ=255)

                                                                                                                                                                            cTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAAt                                                                                                     <  6:1530357/149‑1 (MQ=255)

                                                                                                                                                                             tACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGta                                                                                                                                                   <  8:537566/102‑1 (MQ=255)

                                                                                                                                                                             tACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGta                                                                                                                                                   >  7:537566/1‑102 (MQ=255)

                                                                                                                                                                               cAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                           <  5:436199/140‑1 (MQ=255)

                                                                                                                                                                               cAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtat                                                                                                  >  7:1345103/1‑149 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtata                                                                                                 >  7:26726/1‑149 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                       <  5:743838/111‑3 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                       >  6:743838/1‑109 (MQ=255)

                                                                                                                                                                                 aaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataa                                                                                                <  5:747304/149‑1 (MQ=255)

                                                                                                                                                                                           aaTTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATtata                                                                                      >  8:739959/1‑149 (MQ=255)

                                                                                                                                                                                             ttCAATATATTGCAGAGATGGTGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAg                                                                                    <  6:797256/149‑1 (MQ=255)

                                                                                                                                                                                                 atatatTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTaa                                                                                <  7:339117/149‑1 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaa                                                                                                                             >  3:260786/1‑98 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                                                                                                                       <  1:253056/104‑1 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATta                                                                                                   >  1:145110/1‑124 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATta                                                                                                   <  3:145110/124‑1 (MQ=255)

                                                                                                                                                                                                             gatgatGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAat                                                                    >  5:1431014/1‑149 (MQ=255)

                                                                                                                                                                                                              atgatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCt                                                                                                                 >  2:545718/1‑103 (MQ=255)

                                                                                                                                                                                                                gatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                                                                                                                       <  2:230981/95‑1 (MQ=255)

                                                                                                                                                                                                                gatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGt                                                                        <  6:1167017/142‑1 (MQ=255)

                                                                                                                                                                                                                gatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGt                                                                        >  5:1167017/1‑142 (MQ=255)

                                                                                                                                                                                                                 atgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                               <  8:1366821/134‑1 (MQ=255)

                                                                                                                                                                                                                 atgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTg                                                                <  5:1057193/149‑1 (MQ=255)

                                                                                                                                                                                                                 atgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTg                                                                <  8:1526220/149‑1 (MQ=255)

                                                                                                                                                                                                                  tgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGt                                                                                                             <  4:545714/103‑1 (MQ=255)

                                                                                                                                                                                                                   gTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGc                                                              >  5:1124951/1‑149 (MQ=255)

                                                                                                                                                                                                                     aGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                               >  7:1654846/1‑98 (MQ=255)

                                                                                                                                                                                                                        cacTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                         <  8:684329/149‑1 (MQ=255)

                                                                                                                                                                                                                        cacTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                         >  7:197329/1‑149 (MQ=255)

                                                                                                                                                                                                                        cacTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                         >  7:1469963/1‑149 (MQ=255)

                                                                                                                                                                                                                           tGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaaa                                                                                                                            >  2:648173/1‑79 (MQ=255)

                                                                                                                                                                                                                             gCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGTGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGAtt                                                    <  5:1514044/149‑1 (MQ=255)

                                                                                                                                                                                                                               aaGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataa                                                                                                                        <  4:648168/79‑1 (MQ=255)

                                                                                                                                                                                                                               aaGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATta                                                                                        <  6:1395756/111‑1 (MQ=255)

                                                                                                                                                                                                                               aaGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATta                                                                                        >  5:1395756/1‑111 (MQ=255)

                                                                                                                                                                                                                                aGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTAt                                                 <  6:524580/149‑1 (MQ=255)

                                                                                                                                                                                                                                 gCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATg                                                <  7:455299/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                       <  6:86855/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                       <  8:1639741/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                       <  8:1469963/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                       <  8:13612/149‑1 (MQ=255)

                                                                                                                                                                                                                                               gTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAAt                                  <  6:1514408/149‑1 (MQ=255)

                                                                                                                                                                                                                                                tAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATc                                 <  7:208247/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              tCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCATGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCggg                                                           >  6:1239825/1‑109 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                               >  6:1341945/1‑87 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                               <  5:1341945/87‑1 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAAt                                                                          >  8:1405833/1‑92 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcg                 >  5:1527407/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcg                 >  6:110718/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcg                 >  8:359479/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 tCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcgt                >  8:612843/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   aCTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataa                                                                                                <  6:1627470/67‑1 (MQ=255)

                                                                                                                                                                                                                                                                   aCTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTTTGCATTTTTTAGTAATCCTGAAACTCTCCGTCGTAt              >  7:1052729/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                     tctcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                               >  7:782066/1‑82 (MQ=255)

                                                                                                                                                                                                                                                                     tctcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTa            >  7:100257/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                       tcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGc          <  5:1248985/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGATGAGTTAATTATTATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGc          >  8:1239980/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                         aaaccACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCa        >  8:1528742/4‑149 (MQ=255)

                                                                                                                                                                                                                                                                         aaGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCa        >  5:1230262/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                           gCCACATATACGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataatataa                                                                                           >  7:1538410/1‑64 (MQ=39)

                                                                                                                                                                                                                                                                            ccACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTg     >  8:413395/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                            ccACCTATATGTGGCTTTATTTTTAAAAAAATAATAACCTTGGGTGACGTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTAGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTg     <  8:1052729/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                             cACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGa    <  8:94524/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                             cACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGa    <  7:372317/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                              aCGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGAttt                                                   <  7:934595/101‑1 (MQ=255)

                                                                                                                                                                                                                                                                               cGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGAcc  <  5:1717400/149‑1 (MQ=255)

CGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGACC  >  NC_000913/770533‑770952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`