BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	770,810	T→C	6.4%	intergenic (+199/‑648)	mngB → / → cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	770,810	0	T	C	6.4%	537.8 / 16.0	165	intergenic (+199/‑648)	mngB/cydA	alpha‑mannosidase/cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand): ref base T (74/80); new base C (5/6); total (79/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 177 total aligned reads displayed.

CGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGACCAAA  >  NC_000913/770533‑770955

cagaaaaaaTCAAGTTTTAAATATTTCGTGGCTNNNTTTACCCTGCCAGTAACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                                  <  3:290435/274‑1 (MQ=39)

    gAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                                  <  2:60507/275‑1 (MQ=255)

    gAAAATAAAGGGTCAAAACATACTGTGCCTTTTTTTACCGGCCGGTCACGGACGTGAAGTTACCGGCTTGCCTGAGAGACAATCAAACCGAAGCCACTGAGACGCGGCCAGATTGTTTACAAAGGGCGATTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTGTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                                  <  4:482564/275‑1 (MQ=255)

                      ttttctggtccTGTTTTCCCCGCCCAGTCCCGTACCTGCCGTTACCGACTGTCCTTAATATCAATCAAACCGAATCCAAATATGCGCGTCCGAATTGTTGATAAAGGGCGCTTTGTTCATGCCGGATACTGCATGATCGCGTTATTCGGTCTACAAAAGTAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGTGCATCAGGTAATTTTGCTTTTATCTTCACTCTCAAGCCACATATATGTGGCTTTATTTTTAACaa                                                                                                                                <  4:413639/266‑1 (MQ=255)

                      cTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaa                                                                                                                                <  2:362876/275‑1 (MQ=255)

                           tgggCCTTGTTTACCCGTCCAGTCCCGAACTTTGTGTTACCGGCTTGCCTGGATATGAATCAAGCCGAATCCACATATGTGCGGCCAGATTGTTGACTAAGGGCGCTTTGGTCATGCCGGTGACGTCATGAACGCGTTTGTCGGTCTACGAAAGCAGGCAAGTTCAATATAGTGCATAGATTATGTAGACACTTGCAAGCGTCGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTAGATGTGGCTGTATTTTTGACAAaagaa                                                                                                                           <  4:240028/274‑1 (MQ=255)

                                                                                                                                  cgcTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATAtg                                                                                                                                                  <  5:480373/149‑1 (MQ=255)

                                                                                                                                     tttGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTgg                                                                                                                                               <  8:847809/149‑1 (MQ=255)

                                                                                                                                        gTTCATGCCGTATCCGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCtt                                                                                                                                            >  7:435369/1‑149 (MQ=255)

                                                                                                                                         ttCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCttt                                                                                                                                           >  7:1667920/1‑149 (MQ=255)

                                                                                                                                         ttCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCttt                                                                                                                                           >  7:556718/1‑149 (MQ=255)

                                                                                                                                          tCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                          <  8:1253440/149‑3 (MQ=255)

                                                                                                                                            aTGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttgaga                                                                                                                                        <  5:490081/149‑5 (MQ=255)

                                                                                                                                              gCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAtttt                                                                                                                                      <  6:1485375/149‑1 (MQ=255)

                                                                                                                                               ccGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttttt                                                                                                                                     <  5:563722/149‑1 (MQ=255)

                                                                                                                                                 ggATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTaa                                                                                                                                   <  6:467521/149‑1 (MQ=255)

                                                                                                                                                   aTACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACa                                                                                                                                 >  6:1514044/1‑149 (MQ=255)

                                                                                                                                                       ggCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaat                                                                                                                             >  6:116776/1‑149 (MQ=255)

                                                                                                                                                        gCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaata                                                                                                                            >  6:1311226/1‑149 (MQ=255)

                                                                                                                                                           tGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAataata                                                                                                                         >  8:682886/1‑149 (MQ=255)

                                                                                                                                                            gAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAAtaataa                                                                                                                        >  8:339117/1‑149 (MQ=255)

                                                                                                                                                              aCGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAAcc                                                                                                                      >  7:1639741/1‑149 (MQ=255)

                                                                                                                                                                gCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCt                                                                                                                    >  7:19915/1‑149 (MQ=255)

                                                                                                                                                                gCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCt                                                                                                                    <  5:116776/149‑1 (MQ=255)

                                                                                                                                                                  tttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                                  <  5:390323/149‑1 (MQ=255)

                                                                                                                                                                  tttATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                                  <  8:1252479/149‑1 (MQ=255)

                                                                                                                                                                     aTTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTAttt                                                                                                                                       <  8:1655470/125‑1 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                              >  6:428169/1‑149 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                              <  6:1247959/149‑1 (MQ=255)

                                                                                                                                                                      ttCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGa                                                                                                              <  7:139626/149‑1 (MQ=255)

                                                                                                                                                                       tCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                                                                                                                          <  4:64279/136‑1 (MQ=255)

                                                                                                                                                                        cGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGt                                                                                                            >  6:151847/1‑149 (MQ=255)

                                                                                                                                                                               cAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtat                                                                                                     >  7:1345103/1‑149 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtata                                                                                                    >  7:26726/1‑149 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtata                                                                                                    >  6:1074047/1‑149 (MQ=255)

                                                                                                                                                                                aaaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACATATACGTGGCttga                                                                                                                                          >  6:743838/1‑109 (MQ=255)

                                                                                                                                                                                 aaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataa                                                                                                   <  5:747304/149‑1 (MQ=255)

                                                                                                                                                                                 aaaGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataa                                                                                                   <  5:1410566/149‑1 (MQ=255)

                                                                                                                                                                                           aaTTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATtata                                                                                         >  8:739959/1‑149 (MQ=255)

                                                                                                                                                                                            aTTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATaa                                                                                        <  8:435369/149‑1 (MQ=255)

                                                                                                                                                                                             ttCAATATATTGCAGAGATGGTGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAg                                                                                       <  6:797256/149‑1 (MQ=255)

                                                                                                                                                                                             ttCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAg                                                                                       >  7:1526220/1‑149 (MQ=255)

                                                                                                                                                                                                    tatTGCAGAGATGATGTAGACACTGGAAAGCGTAGCGCATCAGGTAATTTTGGGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATATTTATAAGTTAACTa                                                                                >  8:372317/1‑149 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaa                                                                                                                                >  3:260786/1‑98 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                                                                                                                          <  1:253056/104‑1 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATta                                                                                                      <  3:145110/124‑1 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATta                                                                                                      >  1:145110/1‑124 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAAt                                                                             <  8:1392737/149‑1 (MQ=255)

                                                                                                                                                                                                       tGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTATAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAAt                                                                             <  5:467827/149‑1 (MQ=255)

                                                                                                                                                                                                        gCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATg                                                                            >  8:1635457/1‑149 (MQ=255)

                                                                                                                                                                                                          agagATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGtt                                                                          >  8:1465656/1‑149 (MQ=255)

                                                                                                                                                                                                             gatgatGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAat                                                                       >  5:1431014/1‑149 (MQ=255)

                                                                                                                                                                                                              atgatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCt                                                                                                                    >  2:545718/1‑103 (MQ=255)

                                                                                                                                                                                                                gatgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataat                                                                                                                          <  2:230981/95‑1 (MQ=255)

                                                                                                                                                                                                                 atgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTg                                                                   <  8:1526220/149‑1 (MQ=255)

                                                                                                                                                                                                                 atgTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTg                                                                   <  5:1057193/149‑1 (MQ=255)

                                                                                                                                                                                                                   gTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGc                                                                 >  5:1124951/1‑149 (MQ=255)

                                                                                                                                                                                                                     aGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTgg                                                                                                                  >  7:1654846/1‑98 (MQ=255)

                                                                                                                                                                                                                        cacTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                            <  8:684329/149‑1 (MQ=255)

                                                                                                                                                                                                                        cacTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                            >  7:1469963/1‑149 (MQ=255)

                                                                                                                                                                                                                         acTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTaa                                                                                                         >  6:778917/1‑103 (MQ=255)

                                                                                                                                                                                                                         acTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTaa                                                                                                         <  5:778917/103‑1 (MQ=255)

                                                                                                                                                                                                                           tGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACaaa                                                                                                                               >  2:648173/1‑79 (MQ=255)

                                                                                                                                                                                                                             gCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGTGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGAtt                                                       <  5:1514044/149‑1 (MQ=255)

                                                                                                                                                                                                                               aaGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAaataa                                                                                                                           <  4:648168/79‑1 (MQ=255)

                                                                                                                                                                                                                               aaGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATta                                                                                           <  6:1395756/111‑1 (MQ=255)

                                                                                                                                                                                                                                aGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTAt                                                    <  6:524580/149‑1 (MQ=255)

                                                                                                                                                                                                                                 gCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATg                                                   <  7:455299/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                          <  8:1639741/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                          <  8:1469963/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                          <  6:86855/149‑1 (MQ=255)

                                                                                                                                                                                                                                          aTCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTttatta                                          <  8:13612/149‑1 (MQ=255)

                                                                                                                                                                                                                                                tAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATc                                    <  7:208247/149‑1 (MQ=255)

                                                                                                                                                                                                                                                         gTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAActc                           >  7:709691/1‑149 (MQ=255)

                                                                                                                                                                                                                                                           ttATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTTTAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTg                         <  6:240991/149‑1 (MQ=255)

                                                                                                                                                                                                                                                            tATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGc                        <  8:197329/149‑1 (MQ=255)

                                                                                                                                                                                                                                                            tATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGc                        <  5:715525/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              tCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCATGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCggg                                                              >  6:1239825/1‑109 (MQ=255)

                                                                                                                                                                                                                                                               cTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtc                     <  6:1124951/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                                  <  5:1341945/87‑1 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcg                    >  7:364831/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                ttCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcg                    >  5:1527407/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                 tCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGcgtcgt                   >  8:612843/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   aCTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataa                                                                                                   >  5:1627470/1‑67 (MQ=255)

                                                                                                                                                                                                                                                                     tctcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAAc                                                                                  >  7:782066/1‑82 (MQ=255)

                                                                                                                                                                                                                                                                     tctcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTa               >  7:100257/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                       tcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCt                                  >  8:1084838/1‑128 (MQ=255)

                                                                                                                                                                                                                                                                       tcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCt                                  <  7:1084838/128‑1 (MQ=255)

                                                                                                                                                                                                                                                                       tcAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGc             <  5:1248985/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aaGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCa           >  5:1230262/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                           gCCACATATACGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataatataa                                                                                              <  8:1538410/64‑1 (MQ=255)

                                                                                                                                                                                                                                                                           gCCACATATACGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATtataatataa                                                                                              >  7:1538410/1‑64 (MQ=39)

                                                                                                                                                                                                                                                                           gCCACATATACGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGt         >  5:458375/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                            ccACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTg        >  8:413395/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                            ccACCTATATGTGGCTTTATTTTTAAAAAAATAATAACCTTGGGTGACGTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTAGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTg        <  8:1052729/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                             cACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGa       <  8:94524/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                              aCGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGAttt                                                      <  7:934595/101‑1 (MQ=255)

                                                                                                                                                                                                                                                                               cGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGAcc     <  5:1717400/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                                  atatGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGACCaaa  >  7:881839/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                                   tatGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGt                                                            <  6:158004/90‑1 (MQ=255)

CGAGGAAAATCAAGGTTCAAATCTATCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGAATAGCAATCAAACCGAAGCCACATATGCGCGGCCAGATTGTTGACAAAGGGCGCTTTGTTCATGCCGGATACGGCATGAACGCTTTATTCGGTCTACAAAAGCAGGCAAATTCAATATATTGCAGAGATGATGTAGACACTGGCAAGCGTAGCGCATCAGGTAATTTTGCGTTTATCTTCACTCTCAAGCCACGTATATGTGGCTTTATTTTTAACAAAATAATAACCCTGGGTGAGTTAATTATAATATAATTATAAGTTAACTAAATGTTAATATTGGCGGGGTGGATTTATGCCTTTATTAGTAATCCTGAAACTCTGCGTCGTATTAGCCAGTGACCAAA  >  NC_000913/770533‑770955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`