BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	151,522	G→T	5.4%	A26A (GCC→GCA)	yadK ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	151,522	0	G	T	5.4%	1108.2 / 34.8	310	A26A (GCC→GCA)	yadK	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base G (122/171); new base T (9/8); total (131/179)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-02

Only 100 of 315 total aligned reads displayed.

AGAGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACA  >  NC_000913/151261‑151788

                       tAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacaga                                                                                                                                                                                                                                           <  2:100542/272‑1 (MQ=255)

                                       aaTATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGa                                                                                                                                                                                                                                               >  2:193381/1‑252 (MQ=255)

                                           tgcaaCTGATTTATCACATGGCCAGTTTCCTGTAATGCACAATCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTTAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacaga                                                                                                                                                                                                                                           <  4:193378/249‑1 (MQ=255)

                                                     ttAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGAGACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCCATATTACCCTGGTTACTGCTTCCGATTTTGCAGGTACTGTTTATTACATTGGCACCAAGATCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCCAAACATTAATAACAGCAACAGAATTATTCTCTTAATCTGCTTACGTTGa                                                                                                                                                                                                          >  4:357610/1‑275 (MQ=255)

                                                            aTCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    <  2:55247/274‑1 (MQ=255)

                                                                              tGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGa                                                                                                                                                                                  <  1:85059/274‑1 (MQ=255)

                                                                                               tGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacaga                                                                                                                                                                                                                                     >  4:215266/1‑206 (MQ=255)

                                                                                                       aaCTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGt                                                                                                                                                         <  2:680681/274‑1 (MQ=255)

                                                                                                            ttttCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCCATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTCAGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTTTTGTTTCCTGTAAAt                                                                                                                                               >  1:32431/1‑279 (MQ=255)

                                                                                                                     tGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATTTCCTGTCCAGCAATGGCTg                                                                                                                                                                                                                                                                        >  6:25073/1‑149 (MQ=255)

                                                                                                                       gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATGGCTAGa                                                                                                                                                                                                                                                                      >  8:403288/1‑149 (MQ=255)

                                                                                                                       gTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGa                                                                                                                                                                                                                                                                      >  5:615608/1‑149 (MQ=255)

                                                                                                                        ttGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGAc                                                                                                                                                                                                                                                                     <  5:1300871/149‑1 (MQ=255)

                                                                                                                           aTAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAgg                                                                                                                                                                                                                                                                  <  8:919495/149‑1 (MQ=255)

                                                                                                                            tAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGACCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                                                                                                                                                                                                                 <  5:298993/149‑1 (MQ=255)

                                                                                                                                  tCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                                                                                                                                                                                                            >  2:737151/1‑180 (MQ=255)

                                                                                                                                           aCATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                                                                                                                                                                                                                 >  6:1267432/1‑134 (MQ=255)

                                                                                                                                                 ccTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCaa                                                                                                                                                                                                                                                          <  1:108498/135‑1 (MQ=255)

                                                                                                                                                  cTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacaga                                                                                                                                                                                                                                           >  6:79759/1‑149 (MQ=255)

                                                                                                                                                   tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAgga                                                                                                                                                                                                                                                                 >  7:871374/1‑125 (MQ=255)

                                                                                                                                                   tGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaa                                                                                                                                                                                                                                          >  7:1608483/1‑149 (MQ=255)

                                                                                                                                                         atatCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacagaa                                                                                                                                                                                                                                    <  7:1322278/149‑1 (MQ=255)

                                                                                                                                                           atCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAatt                                                                                                                                                                                                                                  >  6:678120/1‑149 (MQ=255)

                                                                                                                                                             cccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                                                                                                                                                                                                                 <  6:1477902/116‑1 (MQ=255)

                                                                                                                                                             cccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGc                                                                                                                                                                                                                                                                 >  5:1477902/1‑116 (MQ=255)

                                                                                                                                                              ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                                                                                                                                               <  7:786595/149‑1 (MQ=255)

                                                                                                                                                              ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                                                                                                                                               <  7:595990/149‑1 (MQ=255)

                                                                                                                                                              ccACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAattatt                                                                                                                                                                                                                               >  6:1528135/1‑149 (MQ=255)

                                                                                                                                                                 ccccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATtctc                                                                                                                                                                                                                            <  5:938703/149‑1 (MQ=255)

                                                                                                                                                                  cccAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTctct                                                                                                                                                                                                                           >  5:1078549/1‑149 (MQ=255)

                                                                                                                                                                      acgacgCCCAGAACAATATTACCCTGGTTACTGATTCCGCTTTTGCCGGTGCTGTTTTTTACATTGACAACAAGGTCAATATCCTGTCCAGCAATGGCTTGACAGGCAATTCAAACCAGTAATGACCGAATCAGAATGATACTCTTCATAAGCTTACGTTGAGTAGGATCATTCTCTAGTCCCTGAATCTGAAAGTCTGTTAGTAATAGCGTCCTGTAAATATGACTGGTGCAGACAACTTACGCTCAGTGACACTGTTTTCTGTTTTAttttt                                                                                          >  4:116083/1‑274 (MQ=255)

                                                                                                                                                                           gCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGACACAGACTTATTCTCTTCATCAGCt                                                                                                                                                                                                                  >  6:880174/1‑149 (MQ=255)

                                                                                                                                                                             cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGacagaaacagaa                                                                                                                                                                                                                                    <  5:165479/129‑1 (MQ=255)

                                                                                                                                                                             cAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAAGTATTCTCTTCATCAGCTTa                                                                                                                                                                                                                >  8:1149819/1‑149 (MQ=255)

                                                                                                                                                                              aaGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTAc                                                                                                                                                                                                               <  5:1610247/149‑1 (MQ=255)

                                                                                                                                                                              aaGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAc                                                                                 <  2:230692/275‑1 (MQ=255)

                                                                                                                                                                                 aTCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCCATTGCTGGACAg                                                                                                                                                                                                                                                                   >  7:1165687/1‑94 (MQ=255)

                                                                                                                                                                                    aaTATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAg                                                                                                                                                                                                         >  5:1420028/1‑149 (MQ=255)

                                                                                                                                                                                      tatTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTg                                                                                                                                                                                                       <  7:1062233/149‑1 (MQ=255)

                                                                                                                                                                                       atTACCCTGGTTACTGATTCCGCTTTTGCATGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg                                                                                                                                                                                                      <  5:1384170/149‑1 (MQ=255)

                                                                                                                                                                                       atTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg                                                                                                                                                                                                      <  8:868637/149‑1 (MQ=255)

                                                                                                                                                                                        ttACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTggg                                                                                                                                                                                                     <  7:1218361/149‑1 (MQ=255)

                                                                                                                                                                                         tACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    <  8:1608483/149‑1 (MQ=255)

                                                                                                                                                                                          aCCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt                                                                                                                                                                                                   <  7:502452/149‑1 (MQ=255)

                                                                                                                                                                                           cccTGGTTACTGATGCCGCTTTTGCAGGGGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATgg                                                                                                                                                                                                                                                                           <  3:151360/76‑1 (MQ=255)

                                                                                                                                                                                                gTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGAt                                                                                                                                                                                                   <  8:539369/143‑1 (MQ=255)

                                                                                                                                                                                                   aCTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCAtatata                                                                                                                                                                                          <  6:1575101/149‑1 (MQ=255)

                                                                                                                                                                                                     tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCACTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATTTGGCTGGTGCAGAAAACTTACCCGCAGTGTCAGTTTTTACGGTTTTATTTTTTTCCCCCACCCGGCGTGCACTCATTggtaaaa                                                          >  4:691067/1‑270 (MQ=255)

                                                                                                                                                                                                     tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCata                                                                                                                                                                                              <  7:971624/143‑1 (MQ=255)

                                                                                                                                                                                                     tGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGt                                                                                                                                                                                        >  7:1141898/1‑149 (MQ=255)

                                                                                                                                                                                                      gATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGtt                                                                                                                                                                                       <  7:1627068/149‑1 (MQ=255)

                                                                                                                                                                                                         tCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTg                                                                                                                                                                     <  4:759098/164‑1 (MQ=255)

                                                                                                                                                                                                          ccGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGa                                                                                                                                                                                                                                                                      >  2:692290/1‑66 (MQ=255)

                                                                                                                                                                                                          ccGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCtt                                                                                                                                                                                                                          <  8:119555/110‑1 (MQ=255)

                                                                                                                                                                                                          ccGCATTTGCAGGTTCTGTTTTTTACATTGGCAACATGGTCAATATCCTGTTCAGCAATGGCTTGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTCTTCTCTTCATCAGCTTACGTTGAGTTGGATGCATATCTAGTTCCTGAATCTGTCAGTCTGTTAGTTATAGGt                                                                                                                                                         >  4:468218/1‑175 (MQ=255)

                                                                                                                                                                                                            gCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATACCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGaa                                                                                                                                                                                 <  7:403288/149‑1 (MQ=255)

                                                                                                                                                                                                              ttttGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACt                                                                                                                                                     <  2:468222/175‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  5:145427/149‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  8:1348228/149‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  7:135120/149‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  7:301340/149‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  6:1413696/149‑1 (MQ=255)

                                                                                                                                                                                                                   cAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  6:1326247/149‑1 (MQ=255)

                                                                                                                                                                                                                    aGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAg                                                                                                                                                                         >  5:702229/1‑149 (MQ=255)

                                                                                                                                                                                                                       tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt                                                                                                                                                                                                        <  5:831880/115‑1 (MQ=255)

                                                                                                                                                                                                                       tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt                                                                                                                                                                                                        >  4:290999/1‑115 (MQ=255)

                                                                                                                                                                                                                       tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt                                                                                                                                                                                                        >  6:831880/1‑115 (MQ=255)

                                                                                                                                                                                                                       tGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCt                                                                                                                                                                      <  6:1651794/149‑1 (MQ=255)

                                                                                                                                                                                                                         cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg                                                                                                                                                                                                                                                             >  7:197412/1‑60 (MQ=255)

                                                                                                                                                                                                                         cTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATg                                                                                                                                                                                                                                                             <  8:197412/60‑1 (MQ=255)

                                                                                                                                                                                                                           gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    <  2:291002/115‑1 (MQ=255)

                                                                                                                                                                                                                           gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtta                                                                                                                                                                  >  6:450560/1‑149 (MQ=255)

                                                                                                                                                                                                                           gTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgtta                                                                                                                                                                  <  8:1401593/149‑1 (MQ=255)

                                                                                                                                                                                                                            ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttag                                                                                                                                                                 >  8:609671/1‑149 (MQ=255)

                                                                                                                                                                                                                            ttttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttag                                                                                                                                                                 >  5:432013/1‑149 (MQ=255)

                                                                                                                                                                                                                              ttttACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTgttagtt                                                                                                                                                               <  6:1078549/149‑1 (MQ=255)

                                                                                                                                                                                                                                      tGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    <  1:240504/104‑1 (MQ=255)

                                                                                                                                                                                                                                      tGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    >  3:240504/1‑104 (MQ=255)

                                                                                                                                                                                                                                      tGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCACCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAAt                                                                                                                                                                                >  2:532050/1‑124 (MQ=255)

                                                                                                                                                                                                                                       ggCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTAc                                                                                                                                                      >  5:1197201/1‑149 (MQ=255)

                                                                                                                                                                                                                                        gCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTa                                                                                                                                                                                                                                                   <  4:719906/55‑1 (MQ=255)

                                                                                                                                                                                                                                          tacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGt                                                                                                                                                   >  8:1233142/2‑149 (MQ=255)

                                                                                                                                                                                                                                          aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTg                                                                                                                                                    >  8:1138621/1‑148 (MQ=255)

                                                                                                                                                                                                                                          aacaaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCACCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGt                                                                                                                                                                            <  4:532046/124‑1 (MQ=255)

                                                                                                                                                                                                                                             aaGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTaaa                                                                                                                                                <  8:1476882/149‑1 (MQ=255)

                                                                                                                                                                                                                                                  cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTgg                                                                                                                                                                                                      <  1:148632/90‑1 (MQ=255)

                                                                                                                                                                                                                                                  cAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTgg                                                                                                                                           <  6:1352228/149‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGt                                                                                                                                                                                                        >  4:589444/1‑87 (MQ=255)

                                                                                                                                                                                                                                                   aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          <  6:168103/117‑1 (MQ=255)

                                                                                                                                                                                                                                                   aaTATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTaa                                                                                                                                                                          >  5:168103/1‑117 (MQ=255)

                                                                                                                                                                                                                                                       tCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGa                                                                                                                                                                                                    <  2:589448/87‑1 (MQ=255)

                                                                                                                                                                                                                                                       tCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGt                                                                                                                                      >  5:1333950/1‑149 (MQ=255)

                                                                                                                                                                                                                                                              cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCt                                                                                                                                                                              <  6:96644/102‑1 (MQ=255)

                                                                                                                                                                                                                                                              cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCt                                                                                                                                                                              >  5:96644/1‑102 (MQ=255)

                                                                                                                                                                                                                                                              cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaa                                                                                                                               >  7:730530/1‑149 (MQ=255)

                                                                                                                                                                                                                                                              cAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGga                                 <  2:99971/243‑1 (MQ=255)

                                                                                                                                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCatata                                                                                                                                                                                            <  2:316681/87‑1 (MQ=255)

                                                                                                                                                                                                                                                               aGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaaa                                                                                                                              <  8:730530/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                  aaTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACtt                                                                                                                           >  8:1269011/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   aTGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTa                                                                                                                          <  8:1066291/149‑1 (MQ=255)

AGAGCCAGCAGCCATGACACCGCTAAGGGCTCTAAAATCAATATGTAACTGATTTAGCACATCGCCAGTTCCCTGTAATGCACAGTCGGATACAGTGATAGTGAACTCTTTTCCACCTGGTTGATAACTTTCAGGAGTAACATTACCTGAAAAATATCCCACCCCAACGACGCCAAGATCAATATTACCCTGGTTACTGATTCCGCTTTTGCAGGTGCTGTTTTTTACATTGGCAACAAGGTCAATATCCTGTCCAGCAATGGCTGGACAGGCGATGCAAAACAGTAATGACAGAAACAGAATTATTCTCTTCATCAGCTTACGTTGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACA  >  NC_000913/151261‑151788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`