Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,335,684 G→A 5.0% intergenic (+226/‑147) yciX → / → acnA uncharacterized protein/aconitate hydratase 1; aconitase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,335,6840GA 5.0% 1065.3 / 26.7 315intergenic (+226/‑147)yciX/acnAuncharacterized protein/aconitate hydratase 1; aconitase A
Reads supporting (aligned to +/- strand):  ref base G (137/162);  new base A (6/10);  total (143/172)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

Only 100 of 337 total aligned reads displayed.

ATGATCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACC  >  NC_000913/1335439‑1335946
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                      
                  aatTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtt                                                                                                                                                                                                                                                          >  4:124354/1‑242 (MQ=255)
                                                        gacagcgtttAATAAACCGAAGCAATAAAAGCTTGAGTTATATCAAAACAAAATAATGACCGTTAAGGGTGTGGCCTATGATCAACACAAATATGAAAGATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   <  4:175178/268‑1 (MQ=255)
                                                                             gcaATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                                                                                                                                                                                     <  2:141658/188‑1 (MQ=255)
                                                                                                     acacAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGcggcgg                                                                                                                                                                                                                                                                    <  8:107636/149‑1 (MQ=255)
                                                                                                      cacaAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggg                                                                                                                                                                                                                                                                   <  8:182774/149‑1 (MQ=255)
                                                                                                      cacaAAATAATAACCGTTAAGAGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGTGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggg                                                                                                                                                                                                                                                                   <  5:720279/149‑1 (MQ=255)
                                                                                                       acaAAATAATACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGt                                                                                                                                                                                                                                                                 >  6:1165812/1‑149 (MQ=255)
                                                                                                         aaaaTAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGtt                                                                                                                                                                                                                                                                <  8:251764/149‑1 (MQ=255)
                                                                                                         aaaaTAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGtt                                                                                                                                                                                                                                                                >  5:901952/1‑149 (MQ=255)
                                                                                                          aaaTAATAACCGTTAAGGGTGTAGCCTATGATCAACACGAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTCCTGGGCCGACCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCCGCAGCGGGGtca                                                                                                                                                                                                                                                               <  7:1541631/149‑3 (MQ=255)
                                                                                                           aataatAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGt                                                                                                                                                                                                                                                              >  8:1526899/1‑149 (MQ=255)
                                                                                                            ataataACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc                                                                                                                                                                                                                                                             >  6:526504/1‑149 (MQ=255)
                                                                                                                     gTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggg                                                                                                                                                                                                                                                                   >  8:648546/1‑134 (MQ=255)
                                                                                                                      ttAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTGTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggg                                                                                                                                                                                                                                                                   >  8:1608893/1‑133 (MQ=255)
                                                                                                                      ttAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCggg                                                                                                                                                                                                                                                                   <  7:1608893/133‑1 (MQ=255)
                                                                                                                       tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttgc                                                                                                                                                                                                                                                  <  6:896410/149‑1 (MQ=255)
                                                                                                                              gtATCCTNTGTTCAACACAAATNNGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGTTGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                                                                                                                                              <  3:150610/226‑1 (MQ=255)
                                                                                                                              gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                                                                                                                                              >  1:150610/1‑226 (MQ=255)
                                                                                                                               tAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGt                                                                                                                                                                                                                                          <  7:1639640/149‑1 (MQ=255)
                                                                                                                                    tATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtctc                                                                                                                                                                                                                                     >  8:770718/1‑149 (MQ=255)
                                                                                                                                       gATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTctcctc                                                                                                                                                                                                                                  <  8:352491/149‑1 (MQ=255)
                                                                                                                                        aTCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCctct                                                                                                                                                                                                                                 <  6:1214599/149‑1 (MQ=255)
                                                                                                                                        aTCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCctct                                                                                                                                                                                                                                 >  8:830322/1‑149 (MQ=255)
                                                                                                                                        aTCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCctct                                                                                                                                                                                                                                 >  5:103120/1‑149 (MQ=255)
                                                                                                                                          cAACACCAAAATGAAATATGGGTCCTGGATGGGGGCGTTTTCTCTGTGGTTGTCCTTCTGGGCCTAACTCCTCTGGATCATTACTCACTGATCCTTTACCCCGCGGCGGCGGGGTTGTCCTTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACgaga                                                                                                  <  4:449269/274‑1 (MQ=255)
                                                                                                                                           aaCACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                                                                                                                                                                                              >  8:522121/1‑149 (MQ=255)
                                                                                                                                           aaCACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                                                                                                                                                                                              <  6:794993/149‑1 (MQ=255)
                                                                                                                                              acaAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                                                                                                                                                                                              >  8:1286652/1‑146 (MQ=255)
                                                                                                                                                     tGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTgg                                                                                                                                                                                                                    <  5:671077/149‑1 (MQ=255)
                                                                                                                                                     tGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTgg                                                                                                                                                                                                                    <  5:615896/149‑1 (MQ=255)
                                                                                                                                                         atatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGttt                                                                                                                                                                                                                                        >  5:929652/1‑125 (MQ=255)
                                                                                                                                                         atatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCt                                                                                                                                       <  2:54074/222‑1 (MQ=255)
                                                                                                                                                           atTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttgtt                                                                                                                                                                                                              <  7:338965/149‑1 (MQ=255)
                                                                                                                                                           atTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAcc                                                                                                                                         <  2:383694/218‑1 (MQ=255)
                                                                                                                                                             tGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTAt                                                                                                                                                                                                            <  7:1214295/149‑1 (MQ=255)
                                                                                                                                                              ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTa                                                                                                                                                                                                  >  1:90736/1‑158 (MQ=255)
                                                                                                                                                              ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg                                                                                                                             >  3:222200/1‑227 (MQ=255)
                                                                                                                                                                tCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgct                                                                                                                                                                                                                                                      >  7:994400/1‑104 (MQ=255)
                                                                                                                                                                  cTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtc                                                                                                                                                                                                                                       <  7:792166/117‑1 (MQ=255)
                                                                                                                                                                  cTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAttt                                                                                                                                                                                                                      <  7:1336525/134‑1 (MQ=255)
                                                                                                                                                                   tGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                                                                                                                                                                                      <  6:427519/149‑1 (MQ=255)
                                                                                                                                                                        gggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAc                                                                                                                                                                                                 <  5:1209248/149‑1 (MQ=255)
                                                                                                                                                                          gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGtt                                                                                                                                                                                         >  2:326121/1‑155 (MQ=255)
                                                                                                                                                                              gTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                                                                                                                                                                                     <  4:326117/155‑1 (MQ=255)
                                                                                                                                                                               ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtg                                                                                                                                                                                       >  4:782693/1‑152 (MQ=255)
                                                                                                                                                                                   ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCGTTTGCTTTGGCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgt                                                                                                                                                                                      <  6:1623146/149‑1 (MQ=255)
                                                                                                                                                                                   ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   <  2:4757/152‑1 (MQ=255)
                                                                                                                                                                                   ctctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   <  2:782699/152‑1 (MQ=255)
                                                                                                                                                                                    tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTgcggc                                                                                                                                                                                                                                                                      <  6:355775/68‑1 (MQ=255)
                                                                                                                                                                                      tGTCGATGCTCGTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   >  6:959714/1‑149 (MQ=255)
                                                                                                                                                                                       gTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa                                                                                                                                                                                  <  6:460619/149‑1 (MQ=255)
                                                                                                                                                                                          gATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGt                                                                                                                                                                                                                                          <  6:1327284/90‑1 (MQ=255)
                                                                                                                                                                                          gATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGt                                                                                                                                                                                                                                          >  5:1327284/1‑90 (MQ=255)
                                                                                                                                                                                           aTGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTc                                                                                                                                                                                                                                                             >  6:1665201/1‑70 (MQ=255)
                                                                                                                                                                                                 ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCtttt                                                                                                                                                                                                                              >  8:614273/1‑95 (MQ=255)
                                                                                                                                                                                                 ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttgtt                                                                                                                                                                                                              >  7:879391/1‑111 (MQ=255)
                                                                                                                                                                                                 ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                                                                                                                                                                                      >  5:463537/1‑119 (MQ=255)
                                                                                                                                                                                                     gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAc                                                                                                                                                                    <  8:1368914/149‑1 (MQ=255)
                                                                                                                                                                                                     gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAc                                                                                                                                                                    <  6:1472126/149‑1 (MQ=255)
                                                                                                                                                                                                      ggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAag                                                                                                                                                             <  4:658401/155‑1 (MQ=255)
                                                                                                                                                                                                           aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAATGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTTTCTTTAATATTCATCCTACAGAAACTCAGGGCTTCGGAACCTTGTCCTTAAGGCGGACCTATGTCGTCAACACTCCGAGAAGCCAGTAAGTACACGTTGCAGGGCAAAGATAAAACTTACCACTAATACAGACCACCGCTtgaggc                                >  4:369809/1‑271 (MQ=255)
                                                                                                                                                                                                           aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCttt                                                                                                                                                                             >  1:204758/1‑134 (MQ=255)
                                                                                                                                                                                                           aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                                                                                                                                              <  6:179022/149‑1 (MQ=255)
                                                                                                                                                                                                             ctcctcTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAaga                                                                                                                                                            <  6:313860/149‑1 (MQ=255)
                                                                                                                                                                                                               cctcTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAgaga                                                                                                                                                          <  5:530531/149‑1 (MQ=255)
                                                                                                                                                                                                                ctctGGATCATTACTCAGTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGCTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGCTGTTTGTGTTATCTTTAATATTCCCCCTGAAGAGaa                                                                                                                                                         <  8:1424747/149‑1 (MQ=255)
                                                                                                                                                                                                                ctctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGaa                                                                                                                                                         <  5:977062/149‑1 (MQ=255)
                                                                                                                                                                                                                 tctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGttt                                                                                                                                                                                        <  7:270765/117‑1 (MQ=255)
                                                                                                                                                                                                                 tctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAAt                                                                                                                                                        >  5:1165206/1‑149 (MQ=255)
                                                                                                                                                                                                                  ctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATc                                                                                                                                                       >  7:1284232/1‑149 (MQ=255)
                                                                                                                                                                                                                     gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAgg                                                                                                                                                    <  7:728668/149‑1 (MQ=255)
                                                                                                                                                                                                                      aTCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAggg                                                                                                                                                   <  7:431938/149‑1 (MQ=255)
                                                                                                                                                                                                                          ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGt                                                                                                                                                                                          <  1:244612/106‑1 (MQ=255)
                                                                                                                                                                                                                          ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTc                                                                                                                                               >  7:187794/1‑149 (MQ=255)
                                                                                                                                                                                                                           gaCTCACTGTTTCTTGACCCGCTTGAGGCGTGTNNNTCATTTGCTTTGCAAAAAGGTTTTTCCTCTTTTACTAATTTTGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAAAATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTGATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa            <  3:282811/278‑1 (MQ=255)
                                                                                                                                                                                                                              tCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                            <  4:512092/228‑1 (MQ=255)
                                                                                                                                                                                                                              tCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCa                                                        <  2:687905/232‑1 (MQ=255)
                                                                                                                                                                                                                                aCTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATc                                                                                                                                                       >  2:632918/1‑135 (MQ=255)
                                                                                                                                                                                                                                aCTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAcc                                                                                                                                         >  5:250339/1‑149 (MQ=255)
                                                                                                                                                                                                                                  tGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTg                                                                                                                                                                                                                     >  8:1222152/1‑71 (MQ=255)
                                                                                                                                                                                                                                  tGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   <  7:1404026/105‑1 (MQ=255)
                                                                                                                                                                                                                                  tGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGaa                                                                                                                                                         >  4:269453/1‑131 (MQ=255)
                                                                                                                                                                                                                                    aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAggg                                                                                                                                                   <  4:632913/135‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGt                                                                                                                                     >  5:417631/1‑149 (MQ=255)
                                                                                                                                                                                                                                    aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGt                                                                                                                                     >  7:1509914/1‑149 (MQ=255)
                                                                                                                                                                                                                                      ccTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                                                                                                                                     <  2:269456/131‑1 (MQ=255)
                                                                                                                                                                                                                                       cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAc                                                                                                                                                                    >  7:104149/1‑115 (MQ=255)
                                                                                                                                                                                                                                       cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTc                                                                                                                                    <  6:1065502/147‑1 (MQ=255)
                                                                                                                                                                                                                                       cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt                                                                                                                                  >  5:1667171/1‑149 (MQ=255)
                                                                                                                                                                                                                                          gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTAt                                                                                                                                                                                                            >  2:224319/1‑72 (MQ=255)
                                                                                                                                                                                                                                          gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTaa                                                                                                                               <  8:1509914/149‑1 (MQ=255)
                                                                                                                                                                                                                                          aaCCCCGCCGCAGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAc                                                                                                                                                                    <  5:1631197/111‑1 (MQ=255)
                                                                                                                                                                                                                                           aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAag                                                                                                                              <  6:592831/149‑1 (MQ=255)
                                                                                                                                                                                                                                           aCCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAag                                                                                                                              <  5:173736/149‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                                                                                                                                        <  4:224316/72‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAaggag                                                                                                                           >  6:702376/1‑149 (MQ=255)
                                                                                                                                                                                                                                               cGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAaggagg                                                                                                                          <  6:684320/149‑1 (MQ=255)
                                                                                                                                                                                                                                                gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                                                                                                                                                   <  1:110056/91‑1 (MQ=255)
                                                                                                                                                                                                                                                gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAggagga                                                                                                                         <  5:842086/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     ggcggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCGCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGATCTAt                                                                                                                    <  8:308615/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                      
ATGATCAGCCGAAACAATAATTATCATCATTCTTATTACCCATTTTTAATGAATTAAAGGGCTTTTAATACACCGCAGCAATAACAGCTTGAGTTATCTCAACACAAAATAATAACCGTTAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACC  >  NC_000913/1335439‑1335946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: