Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,795,228 | G→C | 6.0% | intergenic (‑76/+25) | btuC ← / ← ihfA | vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,795,228 | 0 | G | C | 6.0% | 698.5 / 26.3 | 200 | intergenic (‑76/+25) | btuC/ihfA | vitamin B12 ABC transporter permease/integration host factor (IHF), DNA‑binding protein, alpha subunit |
Reads supporting (aligned to +/- strand): ref base G (105/82); new base C (7/5); total (112/88) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01 |
Only 100 of 203 total aligned reads displayed.
CAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCT > NC_000913/1794961‑1795498 | cAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCg < 2:267843/272‑1 (MQ=255) gacgcgcGTACGTGGAAGGCGAATTTGCCAGACGAACAGGTCGCCACGAGGGGTAAACCAGTGACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGAATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTtta < 4:560617/270‑1 (MQ=255) ggagTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCCGATTACTCGTCTTTGGGCGAAGCGTTTTCGATCGGGCtt > 4:186836/1‑274 (MQ=255) tCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGcc > 2:617110/1‑275 (MQ=255) acagacATTGTTAAACGGCGCAAAGGATTAAGAGAGTCGCCAGGAGCATCAAAACTGACAGGAATAATAACTAGGGAATAGTTGGTCGGTGTTGTTGGCGGGAAAGTGTCGGGTTGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTAGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGa < 4:89328/269‑1 (MQ=255) tCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGTTCTGAAGGTccc > 1:31675/1‑276 (MQ=255) aCCGGCGCAAAGGCTTAAGTGAAGCGCCAGCAGCAGCAAAACGGACAGGCATAAGAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcgcg < 2:64768/274‑1 (MQ=255) cGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGt > 2:89328/1‑255 (MQ=255) cGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCg > 2:548059/1‑275 (MQ=255) aaGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATACCCAGCGAATATTTTGTCGCTGTGTTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTa < 2:170303/275‑1 (MQ=255) aGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGcc > 1:203702/1‑279 (MQ=255) aGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGGAATGGGAATATCCt > 2:218666/1‑275 (MQ=255) gtCAAAAGTGACAGGCATAAGAACCTGCGAATATTTTTTCGCTGTTGTTGGAGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTc < 2:431748/273‑1 (MQ=255) tCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGc > 7:989494/1‑149 (MQ=255) tCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGc > 6:191092/1‑149 (MQ=255) aaaaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑t > 7:747652/1‑149 (MQ=255) aaaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑tt < 6:514617/149‑1 (MQ=255) aaCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑ttt < 6:1623272/149‑1 (MQ=255) aCTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCGGAAGTGTCATGGCGTTGATTTTACGGTGCCTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCC‑tttt < 7:44874/149‑1 (MQ=255) tGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCTCTGCGGCC‑tttttt < 5:836388/149‑2 (MQ=255) aCAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTttagt < 6:1132013/149‑1 (MQ=255) tgcttattaaCCAGTGATGATTTTGTTGTTGTTTTTGGTGGGCAAGTGTAAGCATGGTCGCCGTTATGCTGAAGTGTTATNGCGTTGTTTTTACGGTGACTATTCGACAGTGAAAAGAAAAAAGGCCGCAGAGAGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCg < 3:215999/271‑1 (MQ=255) gCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGa > 5:331850/1‑149 (MQ=255) cATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGGATATCCTCGCCCGTTTTCGGGTTCCGTccccgc > 4:271809/1‑271 (MQ=255) aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCg > 5:1217408/1‑123 (MQ=255) aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTc > 1:122429/1‑259 (MQ=255) aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCg > 1:215999/1‑266 (MQ=255) aGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTCAGATCAGATTACTc < 6:331850/149‑1 (MQ=255) aGCGAATATTTTGTCGCTGTTGTTAGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTAGATTTTACGGTGACTCTTCGACAGTAAGAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATGAGATTACTc > 7:1425965/1‑149 (MQ=255) ctaaTATTTTGTCGGGGTGGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGGGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACg < 1:211090/277‑1 (MQ=255) gAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTtta > 4:780692/1‑129 (MQ=255) gAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGcc < 4:19237/214‑1 (MQ=255) aaTATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcgcg < 2:541782/211‑1 (MQ=255) atTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTttagtta < 2:780698/129‑1 (MQ=255) atTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTg > 6:576631/1‑149 (MQ=255) ttttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCCTGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGGCTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTgg < 7:1028068/149‑1 (MQ=255) ttttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTgg < 6:267836/149‑1 (MQ=255) tttGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGATAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTggg > 7:1398126/1‑149 (MQ=255) gTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGaa > 4:352992/1‑191 (MQ=255) tCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGaa < 7:1251117/149‑1 (MQ=255) cGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAg < 5:576631/149‑1 (MQ=255) cTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTggg > 7:308497/1‑142 (MQ=255) tgttgGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGtttt < 6:1022482/149‑1 (MQ=255) cGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAccc < 5:1545759/149‑1 (MQ=255) gtgtCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATATTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCtttt < 8:1108973/149‑1 (MQ=255) cAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAAc < 8:71830/149‑1 (MQ=255) gCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTttagtta < 8:133886/97‑1 (MQ=255) gCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTttagtta > 7:133886/1‑97 (MQ=255) gCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACtt < 6:962661/149‑1 (MQ=255) tGGGATCCGTTCTGCTGAAGTTTCATGGCGTTAATTTTACGGTGACTCTTGGACAGTGAAAAGAGAAAAGGACGCAGAGAGGCC‑TTTGTAGTTGGATCAGATTACTCGTCTTTGGGCGAAGCGGTTTCGACCCGGCTTTGTAACGTCTGCCAGGGTCTGAATGGCAACACGCGCCCGGCGTGAATGGGAGTCTCCACGCCCGATTTGGGGGTTCGGCCAGGGCGTTGCTTGTTGTCACGCAGATCGAAGTTACAAAACACAGAGAGgttacactg > 4:623747/1‑269 (MQ=255) gTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGTCTTTTTAACTTCTGcc > 6:1321532/1‑149 (MQ=255) tATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGtttt > 8:1251611/1‑124 (MQ=255) tATCCGTGCTGCTGAAGTGTCATGCCTTTGATTTTACGGTGACTCGTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTCTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGAccc < 1:283609/130‑1 (MQ=255) aTCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCg > 6:1444244/1‑149 (MQ=255) tCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCgg > 7:1174525/1‑149 (MQ=255) gTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTc > 8:817979/1‑149 (MQ=255) gTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTATTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCTGGTc > 5:1351547/1‑149 (MQ=255) ctgctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCACGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCaaaa > 2:383622/1‑247 (MQ=255) gctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAg > 5:748666/1‑149 (MQ=255) gctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGAGTCTTTTCACGACAATCGAAGTTCACAAACCCAGAGAGTTTCACCTTTTCGCCGTTTTc > 4:117419/1‑274 (MQ=255) ctgACGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCGGGCTTTTTAACTTCTACCCGGGTCTGCAGGTCACCACGCGCCGTGCTGGAATGGTACTAACCTCGCCCGTTTTCGGGTTACGTCCCGCGCGTTTATTCTTATCACACAGACAGAAGTTACCAAAACCAGAGCGTTTAACCTGTTCGCCCTTTTc > 4:65123/1‑273 (MQ=255) ctgAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGc < 6:65400/69‑1 (MQ=255) aGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcgcg < 2:426812/155‑1 (MQ=255) gtgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATc > 4:127177/1‑79 (MQ=255) tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTg > 7:1466382/1‑94 (MQ=255) tgtCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTg < 8:1466382/94‑1 (MQ=255) tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCg > 4:676353/1‑107 (MQ=255) tCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcg > 8:188076/1‑149 (MQ=255) cATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACg < 2:67488/216‑1 (MQ=255) aTGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCAcgcg > 7:213760/1‑149 (MQ=255) gTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCggg > 5:763249/1‑126 (MQ=255) gTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTg > 5:647825/1‑149 (MQ=255) gATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTCATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGAGCGAAGGTAcc > 1:194881/1‑222 (MQ=255) gATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCtt > 2:189403/1‑275 (MQ=255) aTTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGt > 7:1275048/1‑149 (MQ=255) tACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATg < 5:1441946/149‑1 (MQ=255) aCGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATgg < 6:458613/149‑1 (MQ=255) ggTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGa > 7:1626038/1‑149 (MQ=255) aCTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAAt > 1:24759/1‑141 (MQ=255) aCTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAAt < 3:24759/141‑1 (MQ=255) tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc > 7:168606/1‑149 (MQ=255) tctTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATcc > 5:1697821/1‑149 (MQ=255) cGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGc < 7:422558/149‑1 (MQ=255) tGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTCGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTCACTTCTGCCCGGGTCTGAAGGTCACTACGCGCCGTGCTGTAATGGGAATATCCTTGCCCGATTTCGGGCTACGTCCCGTGCGGTGATCCTTATCACGCAGATCGAAGTTACTAAAACCAGAGAGTTTACCCTGTTGGTCGTTCTCCTATGCGCTACGGACATCTTCCAAAAAACATTAAACCAATTCt > 4:431140/1‑274 (MQ=255) tGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTaa > 8:443524/1‑87 (MQ=255) tGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATggg > 7:1172427/1‑132 (MQ=255) tGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATggg < 8:1172427/132‑1 (MQ=255) tGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGttt > 8:348993/1‑149 (MQ=255) gaaaagaaaaAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGtttt > 6:1187835/1‑149 (MQ=255) aaaagaaaaAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGccc < 2:345018/94‑1 (MQ=255) aagaaaaAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGc < 2:471884/119‑1 (MQ=255) taaaaaGGCCGCTCTGCGGCCTTTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGt > 8:1198289/2‑149 (MQ=255) aaaaaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt < 8:168606/149‑1 (MQ=255) aaaaaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGtt < 5:427049/149‑1 (MQ=255) aaaaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGGCTTTGGGCGAAGCGTGTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTGTCGGGTTa > 6:211261/1‑149 (MQ=255) aaaGGCCGCTCTGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCgg > 8:310300/1‑143 (MQ=255) aaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAGGCGTTTTCGACCCGGCTTTTTAACTTCTACCCGGGTCTGAAGGTAACCACGCGCCGTGCTGTAATGGGAATCTCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTGCATTCTTATCCCCCACATCGAAGTTACCAAAAACAACGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCTACGGTACTCTTCGAATAACAGTTCAACCAGTTCttttgacacacc > 4:484917/1‑266 (MQ=255) aaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACg > 6:534794/1‑149 (MQ=255) aaaGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGATTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACTACGCGACGTGCTGTAATGGGAATATCCTAGCCCGTTTTCGGGTTACg > 7:663988/1‑149 (MQ=255) gCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTAc < 7:870731/141‑1 (MQ=255) | CAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAGGAGTAAACCAGTCACCTGGCGAGATCCATTGTTCACCGGCGCAAAGGCTTAAGAGAAGCGCCAGCAGCATCAAAACTGACAGGCATAATAACCAGCGAATATTTTGTCGCTGTTGTTGGCGGGCAAGTGTCAGCATGGTATCCGTTCTGCTGAAGTGTCATGGCGTTGATTTTACGGTGACTCTTCGACAGTGAAAAGAAAAAAGGCCGCAGAGCGGCC‑TTTTTAGTTAGATCAGATTACTCGTCTTTGGGCGAAGCGTTTTCGACCCGGCTTTTTAACTTCTGCCCGGGTCTGAAGGTCACCACGCGCCGTGCTGTAATGGGAATATCCTCGCCCGTTTTCGGGTTACGTCCCGGGCGTTGATTCTTATCACGCAGATCGAAGTTACCAAAACCAGAGAGTTTCACCTGTTCGCCGTTTTCCAGAGCGCGACGGATCTCTTCGAAAAACAGTTCAACCAGTTCTTTGGCATCCCGCTTGCT > NC_000913/1794961‑1795498 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |