Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,797,388 G→T 8.0% P186Q (CCG→CAG)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,3880GT 8.0% 720.9 / 33.6 212P186Q (CCG→CAG) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (101/93);  new base T (3/14);  total (104/108)
Fisher's exact test for biased strand distribution p-value = 9.62e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 220 total aligned reads displayed.

ATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGGCAGAACAGC  >  NC_000913/1797118‑1797656
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                             
tgcccgccTGCAATGGTATCTTTATCAAAGTCTGGGACCGGTGGGTACATTTCGAGCATAACATAGTTGGGGAAGTCAACAAAGGCATCGATAGAACGGAGCCCGCAACGACGCAGTTTTTCTTCCATCCACAGCGGAGTTTGCGCTTTAACGTGAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGTACCAACCCGAACGATTTCCGGTTGAACCAGCGGCAg                                                                                                                                                                                                                                                                           <  4:678356/272‑1 (MQ=255)
      ccTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTCCCGGTTGAACCAGCGGCAGCTGGt                                                                                                                                                                                                                                                                      >  2:551075/1‑274 (MQ=255)
                           atagcggagaTCGGCTGGCCCAGTGCGAGCAGGAAATAGTAGGGGTCTTCAACAAACGCAACGAAGTAACGGATACCGCAACGACGCAGTTTTGCTTTAATCGCCAGCGGAGTTGGCGGTTGAACGTTAATGCCGTTTACCCCACGGCCAAGAGAGCGCGGGCAGGCTTCCGGCGCGTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGAAACCATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc                                                                                                                                                                                                                                                <  4:647007/266‑1 (MQ=255)
                            aaGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCCCGGCAACGTCAcgcc                                                                                                                                                                                                                                                 >  2:231090/1‑272 (MQ=255)
                            aaGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgcg                                                                                                                                                                                                                                                 >  2:469909/1‑273 (MQ=255)
                                                                     gTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCTACTGTAATCGGCAGCGTTCCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCACCGGCACCTGGTTCCGCCCGGCAACCTCACCCCGAACACCAATGATGCCTAAGCAGTCGGGCCGGTTTGGCATCAc                                                                                                                                                                                                      >  4:377948/1‑275 (MQ=255)
                                                                                          aTAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCCCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCCAGCTGATTTCGATTGtgttggc                                                                                                                                                                                 >  2:598115/1‑273 (MQ=255)
                                                                                                                          tttcATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               >  6:1008630/1‑149 (MQ=255)
                                                                                                                          tttcATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               >  5:629313/1‑149 (MQ=255)
                                                                                                                          tttcATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               >  7:1314295/1‑149 (MQ=255)
                                                                                                                          tttcATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               >  8:638850/1‑149 (MQ=255)
                                                                                                                              aTCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAg                                                                                                                                                                                                                                                                           >  5:297901/1‑149 (MQ=255)
                                                                                                                                 cacaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTg                                                                                                                                                                                                                                                                        >  7:447452/1‑149 (MQ=255)
                                                                                                                                  acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                       <  8:1265193/149‑1 (MQ=255)
                                                                                                                                  acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                       <  5:530757/149‑1 (MQ=255)
                                                                                                                                  acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                       <  6:797188/149‑1 (MQ=255)
                                                                                                                                  acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                       >  6:145356/1‑149 (MQ=255)
                                                                                                                                    aGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGgcg                                                                                                                                        >  2:376318/1‑274 (MQ=255)
                                                                                                                                     gCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTc                                                                                                                                                                                                                                                                    <  6:272450/149‑1 (MQ=255)
                                                                                                                                     gCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTc                                                                                                                                                                                                                                                                    <  8:559983/149‑1 (MQ=255)
                                                                                                                                       ggAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaa                                                                                                                                                                                                                                                                  <  8:292808/149‑2 (MQ=255)
                                                                                                                                        gAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                                                                                                                                                 <  7:638488/149‑3 (MQ=255)
                                                                                                                                        gAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                                                                                                                                                 <  6:15880/149‑3 (MQ=255)
                                                                                                                                          gTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                                                                                                                                                 >  5:987142/1‑145 (MQ=255)
                                                                                                                                                gcTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAAc                                                                                                                                                                                                                                                         <  5:1579749/149‑1 (MQ=255)
                                                                                                                                                  tttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGt                                                                                                                                                                                                                                                       >  7:1551057/1‑149 (MQ=255)
                                                                                                                                                   ttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTc                                                                                                                                                                                                                                                      >  7:823106/1‑149 (MQ=255)
                                                                                                                                                    tAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               >  6:169484/1‑123 (MQ=255)
                                                                                                                                                    tAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCg                                                                                                                                                                                                                                                                               <  5:169484/123‑1 (MQ=255)
                                                                                                                                                     aaCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                                                                                                                                                                                                    <  5:439011/149‑1 (MQ=255)
                                                                                                                                                     aaCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                                                                                                                                                                                                    <  6:297901/149‑1 (MQ=255)
                                                                                                                                                      aCGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcg                                                                                                                                                                                                                                                   >  8:1529895/1‑149 (MQ=255)
                                                                                                                                                             tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAaca                                                                                                                                                                                                                                            >  7:777217/1‑149 (MQ=255)
                                                                                                                                                              gCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                                                                                                                                           >  5:598093/1‑149 (MQ=255)
                                                                                                                                                              gCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                                                                                                                                           >  7:1503737/1‑149 (MQ=255)
                                                                                                                                                                 ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                        >  8:1079012/1‑149 (MQ=255)
                                                                                                                                                                 ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                        <  6:1065591/149‑1 (MQ=255)
                                                                                                                                                                     aCCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                                                                                                                                                    <  5:1100980/149‑1 (MQ=255)
                                                                                                                                                                         cacGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCt                                                                                                                                                                                                                                >  7:222087/1‑149 (MQ=255)
                                                                                                                                                                         cacGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCt                                                                                                                                                                                                                                <  5:915407/149‑1 (MQ=255)
                                                                                                                                                                           cGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTaa                                                                                                                                                                                                                              <  5:1388608/149‑1 (MQ=255)
                                                                                                                                                                            ggCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                       <  8:1423105/107‑1 (MQ=255)
                                                                                                                                                                              ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                                                                                                                                                    >  7:1182500/1‑140 (MQ=255)
                                                                                                                                                                               cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGc                                                                                                                                                                                                                                  <  8:67454/141‑1 (MQ=255)
                                                                                                                                                                               cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGc                                                                                                                                                                                                                                  >  7:67454/1‑141 (MQ=255)
                                                                                                                                                                               cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAg                                                                                                                                                                                                                          >  5:756826/1‑149 (MQ=255)
                                                                                                                                                                                  gttAACGCGGGCAGGCTGCCGGCGCTTCAACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCGGACAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                                                                         <  2:445884/272‑1 (MQ=255)
                                                                                                                                                                                   aTAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCgg                                                                                                                                                                                                                      <  5:417067/149‑1 (MQ=255)
                                                                                                                                                                                           ggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGttt                                                                                                                                                                                                              <  6:1299919/149‑1 (MQ=255)
                                                                                                                                                                                            gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTg                                                                                                                                                                                                             >  6:394216/1‑149 (MQ=255)
                                                                                                                                                                                            gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCagag                                                                               >  2:35046/1‑274 (MQ=255)
                                                                                                                                                                                               ggCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCg                                                                                                                                                                                                          >  7:478205/1‑149 (MQ=255)
                                                                                                                                                                                                  ttCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCa                                                                                                                                                                                                       >  7:246966/1‑149 (MQ=255)
                                                                                                                                                                                                  ttCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCa                                                                                                                                                                                                       >  6:614550/1‑149 (MQ=255)
                                                                                                                                                                                                  ttCCGGCGCTTCGACTGTAATCGGCAGCGTGCCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                                                                                                                                                     >  5:1412255/1‑87 (MQ=255)
                                                                                                                                                                                                       gcgcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACg                                                                                                                                                                                                     <  8:491787/146‑1 (MQ=255)
                                                                                                                                                                                                         gcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt                                                                                                                                                                                                <  5:675938/149‑1 (MQ=255)
                                                                                                                                                                                                           ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAttt                                                                                                                                                                                              >  6:983528/1‑149 (MQ=255)
                                                                                                                                                                                                           ttCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                                                                                                                                                      <  2:702144/173‑1 (MQ=255)
                                                                                                                                                                                                              gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa                                                                                                                                                                                           <  6:678946/149‑1 (MQ=255)
                                                                                                                                                                                                              gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGACACGGTTTGGCGTCACGCTGATTTCGa                                                                                                                                                                                           <  8:440464/149‑1 (MQ=255)
                                                                                                                                                                                                                cTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATg                                                                                                                                                                                         <  7:1246440/149‑1 (MQ=255)
                                                                                                                                                                                                                  gTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGgt                                                                                                                                                                                       <  7:1265483/149‑1 (MQ=255)
                                                                                                                                                                                                                   tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATg                                                                                                                                                                                         >  6:763223/1‑146 (MQ=255)
                                                                                                                                                                                                                   tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTAGCGTCACGCTGATTTCGATGgtg                                                                                                                                                                                      >  7:165185/1‑149 (MQ=255)
                                                                                                                                                                                                                     agcGGCAGCGTGTCGCCGATGGACGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGGCACGCGCAACAACATCGATGCCTAAGCAGTCGGCACGGTGTGGCGTCACGCTGATTTCGATGgttgt                                                                                                                                                                                    <  6:1207635/147‑4 (MQ=255)
                                                                                                                                                                                                                     aTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgtt                                                                                                                                                                                    <  8:1065120/149‑1 (MQ=255)
                                                                                                                                                                                                                      tCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgttg                                                                                                                                                                                   <  7:1521756/149‑1 (MQ=255)
                                                                                                                                                                                                                          cAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcat                                                                                                                                                                               <  8:173684/149‑1 (MQ=255)
                                                                                                                                                                                                                           aGCGTGTCGTCGATGGTCGCACCACCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGCTCAGCACGGCCACGTCACCCGCAACACCATCGCTGCCTCAGCAGTCGGCCCGCTTTGGCATCACGCTGATTTCGATTCTGTTTTCATCAAGTTTCAGGTATTCACGGATTTCGGTCCCAACCGGCCCATCCTCCGTCATTTCGATATTTCCGCTGATGACGGCAGAAAGCCCCTGTTCAGAACAGGGTCACACCTTCCCtttcaccaggtccc                                                >  4:469614/1‑262 (MQ=255)
                                                                                                                                                                                                                           aGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatc                                                                                                                                                                              <  7:1079012/149‑1 (MQ=255)
                                                                                                                                                                                                                            gCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCATTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca                                                                                                                                                                             >  6:1264050/1‑149 (MQ=255)
                                                                                                                                                                                                                             cGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCaa                                                                                                                                                                            <  8:823106/149‑1 (MQ=255)
                                                                                                                                                                                                                                gtcgtcGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTATCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGaaa                                                                                             >  2:168991/1‑224 (MQ=255)
                                                                                                                                                                                                                                 tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGttt                                                                                                                                                                        >  6:1408997/1‑149 (MQ=255)
                                                                                                                                                                                                                                  cgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACgg                                                                                                                                                           >  4:285765/1‑161 (MQ=255)
                                                                                                                                                                                                                                   gtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCa                                                                                                                                                                      <  6:598093/149‑1 (MQ=255)
                                                                                                                                                                                                                                    tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                                                                                                                                                                                                    >  8:393639/1‑70 (MQ=255)
                                                                                                                                                                                                                                          gTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTc                                                                                                                                                               >  6:813148/1‑149 (MQ=255)
                                                                                                                                                                                                                                              cACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                     >  8:327503/1‑139 (MQ=255)
                                                                                                                                                                                                                                                 cAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGc                                                                                                                                                                                                           >  7:367120/1‑98 (MQ=255)
                                                                                                                                                                                                                                                 cAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGata                                                                                                                                                        >  5:1491814/1‑149 (MQ=255)
                                                                                                                                                                                                                                                    ccGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCCGAATTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCg                                                                                                                                                     >  5:622316/1‑149 (MQ=255)
                                                                                                                                                                                                                                                      ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCGACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                   <  5:330144/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                   <  7:1418939/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                   <  5:652488/149‑1 (MQ=255)
                                                                                                                                                                                                                                                      ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                   <  5:786390/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                   <  8:1199184/147‑1 (MQ=255)
                                                                                                                                                                                                                                                          cGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCa                                                                                                                                               <  7:734500/149‑1 (MQ=255)
                                                                                                                                                                                                                                                           gATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                        <  5:100724/59‑1 (MQ=255)
                                                                                                                                                                                                                                                           gATTTCCGATTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATAGCGGTGCCaa                                                                                                                                              >  8:735274/1‑149 (MQ=255)
                                                                                                                                                                                                                                                             tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATc                                                                                                                                            <  8:1554853/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                    ttGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCAt                                                                                                                                     >  8:140483/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                     tGAACCAGCGGCAGCGGGTTCAGCACGGCAACGTCACGCGCAACACCTATGAAGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCACTCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATTCCCAGTTCAGCGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTATCGCCGCTTTAATTTTGAAATCACCAGGCAGAACAGc  >  1:111264/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                      gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                                                                                                                                                                                          >  5:1021149/1‑94 (MQ=255)
                                                                                                                                                                                                                                                                      gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCAAATCGGCGCATcc                                                                                                                                   <  6:1610419/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTACTCACGGATATCGGTGCCAATCGGCGCATcc                                                                                                                                   <  5:1264050/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCgg                                                                                                                                          >  5:119868/1‑139 (MQ=255)
                                                                                                                                                                                                                                                                            gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                     <  7:525391/109‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggc                                                                                                                             >  6:1152712/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                             
ATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGGCAGAACAGC  >  NC_000913/1797118‑1797656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: