Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,797,391 A→C 8.0% L185R (CTG→CGG)  pheT ← phenylalanine tRNA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,797,3910AC 8.0% 692.0 / 45.7 213L185R (CTG→CGG) pheTphenylalanine tRNA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base A (101/95);  new base C (3/14);  total (104/109)
Fisher's exact test for biased strand distribution p-value = 9.87e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01

Only 100 of 215 total aligned reads displayed.

CCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGGCAGAACAGCA  >  NC_000913/1797121‑1797657
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                           
                        atagcggagaTCGGCTGGCCCAGTGCGAGCAGGAAATAGTAGGGGTCTTCAACAAACGCAACGAAGTAACGGATACCGCAACGACGCAGTTTTGCTTTAATCGCCAGCGGAGTTGGCGGTTGAACGTTAATGCCGTTTACCCCACGGCCAAGAGAGCGCGGGCAGGCTTCCGGCGCGTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGAAACCATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACgcgc                                                                                                                                                                                                                                                 <  4:647007/266‑1 (MQ=255)
                         aaGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCCCGGCAACGTCAcgcc                                                                                                                                                                                                                                                  >  2:231090/1‑272 (MQ=255)
                         aaGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgcg                                                                                                                                                                                                                                                  >  2:469909/1‑273 (MQ=255)
                                                                  gTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCTACTGTAATCGGCAGCGTTCCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCACCGGCACCTGGTTCCGCCCGGCAACCTCACCCCGAACACCAATGATGCCTAAGCAGTCGGGCCGGTTTGGCATCAc                                                                                                                                                                                                       >  4:377948/1‑275 (MQ=255)
                                                                  gTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                                                                                                                                                                                                       >  2:61212/1‑275 (MQ=255)
                                                                                                                           aTCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAg                                                                                                                                                                                                                                                                            >  5:297901/1‑149 (MQ=255)
                                                                                                                               acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                        >  6:145356/1‑149 (MQ=255)
                                                                                                                               acaGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                        >  8:1676284/1‑149 (MQ=255)
                                                                                                                                caGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGt                                                                                                                                                                                                                                                                       <  7:62243/149‑1 (MQ=255)
                                                                                                                                caGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGCTGAACCAGCTGCCGCTGGt                                                                                                                                                                                                                                                                       <  8:238000/149‑1 (MQ=255)
                                                                                                                                 aGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGgcg                                                                                                                                         >  2:376318/1‑274 (MQ=255)
                                                                                                                                  ttggAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc                                                                                                                                                                                                                                                                     >  6:286552/3‑149 (MQ=255)
                                                                                                                                  gCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTc                                                                                                                                                                                                                                                                     >  6:786390/1‑149 (MQ=255)
                                                                                                                                    ggAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaa                                                                                                                                                                                                                                                                   <  8:292808/149‑2 (MQ=255)
                                                                                                                                    ggAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaa                                                                                                                                                                                                                                                                   <  7:857123/149‑2 (MQ=255)
                                                                                                                                       gTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGTTCaac                                                                                                                                                                                                                                                                  <  6:987142/147‑3 (MQ=255)
                                                                                                                                             gcTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAAc                                                                                                                                                                                                                                                          >  6:1388608/1‑149 (MQ=255)
                                                                                                                                             gcTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAAc                                                                                                                                                                                                                                                          <  5:1579749/149‑1 (MQ=255)
                                                                                                                                             gcTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAAc                                                                                                                                                                                                                                                          <  5:692729/149‑1 (MQ=255)
                                                                                                                                                ttAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTc                                                                                                                                                                                                                                                       <  8:1073259/149‑1 (MQ=255)
                                                                                                                                                    cGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAcgc                                                                                                                                                                                                                                                   <  6:893472/149‑1 (MQ=255)
                                                                                                                                                         aTGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCTACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCCCGGCTATCGTTTCCAATCGGCGCATCCGCAGGCAGTTCGAAAATGcc                                                                                                                >  2:582052/1‑275 (MQ=255)
                                                                                                                                                          tGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAaca                                                                                                                                                                                                                                             >  7:1397985/1‑149 (MQ=255)
                                                                                                                                                           gCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                                                                                                                                            >  7:1503737/1‑149 (MQ=255)
                                                                                                                                                           gCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAacac                                                                                                                                                                                                                                            >  5:598093/1‑149 (MQ=255)
                                                                                                                                                              ttttACCACACGGCCAAGATAACGCGGGCAGGTTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                         <  7:1550967/149‑1 (MQ=255)
                                                                                                                                                              ttttACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                         <  5:633518/149‑1 (MQ=255)
                                                                                                                                                                  aCCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                                                                                                                                                     <  5:1100980/149‑1 (MQ=255)
                                                                                                                                                                     acacGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGcc                                                                                                                                                                                                                                  >  6:1057831/1‑149 (MQ=255)
                                                                                                                                                                       acGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTa                                                                                                                                                                                                                                <  7:819829/149‑1 (MQ=255)
                                                                                                                                                                        cGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTaa                                                                                                                                                                                                                               <  5:1388608/149‑1 (MQ=255)
                                                                                                                                                                         ggCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                        <  8:1423105/107‑1 (MQ=255)
                                                                                                                                                                         ggCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTgg                                                                                                                                                                                                                                                                        >  7:1423105/1‑107 (MQ=255)
                                                                                                                                                                           ccAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAatgat                                                                                                                                                                                                                                     <  8:1182500/140‑1 (MQ=255)
                                                                                                                                                                            cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGc                                                                                                                                                                                                                                   <  8:67454/141‑1 (MQ=255)
                                                                                                                                                                            cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAg                                                                                                                                                                                                                           >  5:661858/1‑149 (MQ=255)
                                                                                                                                                                            cAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAg                                                                                                                                                                                                                           >  8:126746/1‑149 (MQ=255)
                                                                                                                                                                              aGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTc                                                                                                                                                                                                                         >  7:236978/1‑149 (MQ=255)
                                                                                                                                                                               gttAACGCGGGCAGGCTGCCGGCGCTTCAACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCGGACAGTTCGATAATGCCGCTGTGATCGTCAGAAATGcc                                                                                          <  2:445884/272‑1 (MQ=255)
                                                                                                                                                                                   aCGCGGGCAGGCTTCCGGTGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGt                                                                                                                                                                                                                                                        >  7:1250205/1‑113 (MQ=255)
                                                                                                                                                                                   aCGCGGGCAGGCTTCCGGTGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGt                                                                                                                                                                                                                                                        <  8:1250205/113‑1 (MQ=255)
                                                                                                                                                                                   aCGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCAc                                                                                                                                                                                                                    <  7:505041/149‑1 (MQ=255)
                                                                                                                                                                                        ggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGttt                                                                                                                                                                                                               <  6:1299919/149‑1 (MQ=255)
                                                                                                                                                                                        ggCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGttt                                                                                                                                                                                                               <  6:1588638/149‑1 (MQ=255)
                                                                                                                                                                                         gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTg                                                                                                                                                                                                              <  5:495196/149‑1 (MQ=255)
                                                                                                                                                                                         gCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCagag                                                                                >  2:35046/1‑274 (MQ=255)
                                                                                                                                                                                               ttCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCa                                                                                                                                                                                                        >  7:1115811/1‑149 (MQ=255)
                                                                                                                                                                                               ttCCGGCGCTTCGACTGTAATCGGCAGCGTGCCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCTGCCGCTGGtt                                                                                                                                                                                                                                                                      >  5:1412255/1‑87 (MQ=255)
                                                                                                                                                                                                tCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCAc                                                                                                                                                                                                       <  7:126746/149‑1 (MQ=255)
                                                                                                                                                                                                   ggCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCa                                                                                                                                                                                                                                                                 >  7:747760/1‑88 (MQ=255)
                                                                                                                                                                                                    gcgcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGANGTCGGCACGGTTTGGCGTCACGCNGATTNCGATGGTGTTGTCATCAAGt                                                                                                                                                                           >  4:702139/1‑173 (MQ=255)
                                                                                                                                                                                                      gcTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGAt                                                                                                                                                                                                 >  6:1652737/1‑149 (MQ=255)
                                                                                                                                                                                                         tCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTc                                                                                                                                                                                              >  5:434216/1‑149 (MQ=255)
                                                                                                                                                                                                           gACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGACACGGTTTGGCGTCACGCTGATTTCGa                                                                                                                                                                                            <  8:440464/149‑1 (MQ=255)
                                                                                                                                                                                                            aCTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                                                                                                                                                                                           <  8:1551057/149‑1 (MQ=255)
                                                                                                                                                                                                              tGTAATCGGCAGCGTGTCGTCGATGGTCGCACCACCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATgg                                                                                                                                                                                         >  7:456426/1‑149 (MQ=255)
                                                                                                                                                                                                                tAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATg                                                                                                                                                                                          <  5:763223/146‑1 (MQ=255)
                                                                                                                                                                                                                  agcGGCAGCGTGTCGCCGATGGACGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGGCACGCGCAACAACATCGATGCCTAAGCAGTCGGCACGGTGTGGCGTCACGCTGATTTCGATGgttgt                                                                                                                                                                                     <  6:1207635/147‑4 (MQ=255)
                                                                                                                                                                                                                  aTCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGtgtt                                                                                                                                                                                     <  6:661858/149‑1 (MQ=255)
                                                                                                                                                                                                                        aGCGTGTCGTCGATGGTCGCACCACCCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGCTCAGCACGGCCACGTCACCCGCAACACCATCGCTGCCTCAGCAGTCGGCCCGCTTTGGCATCACGCTGATTTCGATTCTGTTTTCATCAAGTTTCAGGTATTCACGGATTTCGGTCCCAACCGGCCCATCCTCCGTCATTTCGATATTTCCGCTGATGACGGCAGAAAGCCCCTGTTCAGAACAGGGTCACACCTTCCCtttcaccaggtccc                                                 >  4:469614/1‑262 (MQ=255)
                                                                                                                                                                                                                        aGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatc                                                                                                                                                                               >  7:1554853/1‑149 (MQ=255)
                                                                                                                                                                                                                         gCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCATTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGtcatca                                                                                                                                                                              >  6:1264050/1‑149 (MQ=255)
                                                                                                                                                                                                                          cGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCaa                                                                                                                                                                             <  8:823106/149‑1 (MQ=255)
                                                                                                                                                                                                                            tgtCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGt                                                                                                                                                                           >  7:1671377/1‑149 (MQ=255)
                                                                                                                                                                                                                             gtcgtcGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGtt                                                                                                                                                                          <  8:504654/149‑1 (MQ=255)
                                                                                                                                                                                                                             gtcgtcGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTATCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGaaa                                                                                              >  2:168991/1‑224 (MQ=255)
                                                                                                                                                                                                                              tcgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGttt                                                                                                                                                                         >  6:1408997/1‑149 (MQ=255)
                                                                                                                                                                                                                               cgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTc                                                                                                                                                                        <  6:201438/149‑1 (MQ=255)
                                                                                                                                                                                                                               cgtcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACgg                                                                                                                                                            >  4:285765/1‑161 (MQ=255)
                                                                                                                                                                                                                                 tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                                                                                                                                                                                                     >  8:393639/1‑70 (MQ=255)
                                                                                                                                                                                                                                 tcgATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCAc                                                                                                                                                                                                                                                     <  7:393639/70‑1 (MQ=255)
                                                                                                                                                                                                                                           cACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                      >  8:327503/1‑139 (MQ=255)
                                                                                                                                                                                                                                           cACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                      <  7:327503/139‑1 (MQ=255)
                                                                                                                                                                                                                                            aCCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                      <  7:671161/138‑1 (MQ=255)
                                                                                                                                                                                                                                            aCCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGa                                                                                                                                                           >  5:555915/1‑149 (MQ=255)
                                                                                                                                                                                                                                              cAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGata                                                                                                                                                         >  5:1491814/1‑149 (MQ=255)
                                                                                                                                                                                                                                                 ccGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCCGAATTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCg                                                                                                                                                      >  5:622316/1‑149 (MQ=255)
                                                                                                                                                                                                                                                   ggAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCGACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                    <  5:330144/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    gAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTg                                                                                                                                                   >  6:735861/1‑149 (MQ=255)
                                                                                                                                                                                                                                                     aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGt                                                                                                                                                    >  7:1199184/1‑147 (MQ=255)
                                                                                                                                                                                                                                                     aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                                                                                                                                                  <  5:983528/149‑1 (MQ=255)
                                                                                                                                                                                                                                                     aaCGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGc                                                                                                                                                  <  6:603315/149‑1 (MQ=255)
                                                                                                                                                                                                                                                        gATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                         >  6:100724/1‑59 (MQ=255)
                                                                                                                                                                                                                                                        gATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                         <  5:100724/59‑1 (MQ=255)
                                                                                                                                                                                                                                                        gATTTCCGATTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATAGCGGTGCCaa                                                                                                                                               >  8:735274/1‑149 (MQ=255)
                                                                                                                                                                                                                                                          tttCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATc                                                                                                                                             >  8:631746/1‑149 (MQ=255)
                                                                                                                                                                                                                                                               ggTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGgcgc                                                                                                                                        <  5:258315/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ttGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCaa                                                                                                                                                                                                                                         >  7:706390/1‑50 (MQ=255)
                                                                                                                                                                                                                                                                 ttGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCAt                                                                                                                                      >  8:140483/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                  tGAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                      <  6:809188/116‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tGAACCAGCTGCCGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAg                                                                                                                                                                      >  5:809188/1‑116 (MQ=255)
                                                                                                                                                                                                                                                                  tGAACCAGCGGCAGCGGGTTCAGCACGGCAACGTCACGCGCAACACCTATGAAGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCACTCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATTCCCAGTTCAGCGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTATCGCCGCTTTAATTTTGAAATCACCAGGCAGAACAGc   >  1:111264/1‑278 (MQ=255)
                                                                                                                                                                                                                                                                   gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGAt                                                                                                                                                                                           >  5:1021149/1‑94 (MQ=255)
                                                                                                                                                                                                                                                                   gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCAAANCGGCTCATCCGCAGGCCGGTCGATACTTCCGATGTGATCGTCAGCAATGCCCATTTCAGCGAATGATCACAGCATCCCTTNAGACGGTTGCACACGCAGCTTCCCCNCCTCAATTTTTAAATCCCCCCGCCGcacaccc  >  3:171614/1‑275 (MQ=255)
                                                                                                                                                                                                                                                                   gAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTACTCACGGATATCGGTGCCAATCGGCGCATcc                                                                                                                                    <  5:1264050/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ccAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCgg                                                                                                                                           >  5:119868/1‑139 (MQ=255)
                                                                                                                                                                                                                                                                       cAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGa                                                                                                                                                                                            <  6:302257/89‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGCGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcagg                                                                                                                               >  6:498775/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                         gCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCgcaggc                                                                                                                              >  6:1152712/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              aGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTc                                                                                                                         >  8:543840/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                           
CCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCCGGTTGAACCAGCGGCAGCTGGTTCAGCACGGCAACGTCACGCGCAACACCAATGATGCCTAAGCAGTCGGCACGGTTTGGCGTCACGCTGATTTCGATGGTGTTGTCATCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCGCCACGCAGTTTCGCCGCTTTAATTTTGAAATCACCCGGCAGAACAGCA  >  NC_000913/1797121‑1797657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: