Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 8.4% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC 8.4% 1016.5 / 66.5 299intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (137/135);  new base C (10/15);  total (147/152)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01

Only 100 of 304 total aligned reads displayed.

AGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCC  >  NC_000913/1865411‑1865950
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                            
                   ggTTAATGCTTCCCCAACCACCTTTTCGGAACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                                                                                                                                                        <  2:197895/275‑1 (MQ=255)
                                                                 atggcgggggGATGAATGGAGGTTAACCAGAGTTAAACGGCGGGGGGTATGGCAGTAACTTCGTGTTTGCGTTTACTGGAAGCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACGTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTACCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTa                                                                                                                                                                                                          <  4:668199/271‑1 (MQ=255)
                                                                             tCAATGAGGGTTAAACCGAGTTCAATGCCCTCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTGCGCCAATATACCATGTTCCATGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGGTGCATTGTTTCTGCCCAGGTATTGAACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                                                                                                                                                                                          <  1:198607/279‑1 (MQ=255)
                                                                              cAAGGAGGGTTAAACCGAGTTCAATGCCCGAGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTACGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCAGTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                                                                                                <  2:368385/272‑1 (MQ=255)
                                                                                     ggTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                                                                                                                                                                                           <  2:678694/270‑1 (MQ=255)
                                                                                         aaaCCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                                                                                                <  4:171040/261‑1 (MQ=255)
                                                                                               aGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAAt                                                                                                                                                                                                                                                      >  4:587904/1‑201 (MQ=255)
                                                                                                                      atggatttCTGTGTTTACGGTGACTGGCATCTTCGCCCATTTAACCTGTTGCCTGCCTTCCGGGTGGCCGTGTGACAACTCNACGAAATTGAATAATTTTTTGTTGCATTGTTTCCTACCGGCTGTTGCACCACCGTAATCGCAAACAGGGCGTGGCGCCCTTTTTTTATGAAAGAGATGCCGTGGAAAGATGCATTATGAGAATTTGTGGGTTATCCCGGTAGAAATCAGGCCAGTCCAAGATTTATCCGCCATCGGGCTGTCAGTAACTTCATcaga                                                                                                                                                  <  3:210949/273‑1 (MQ=255)
                                                                                                                             tCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTACCCACCATCGGGCTGTCCGTAACTTCAGcagacaga                                                                                                                                              >  4:356254/1‑275 (MQ=255)
                                                                                                                                     tgggcTGACTGGCATCTTCGCCCATATACCTTGTTCCCTGCCCTACGGCTGGCAGTGAGACATCTCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGt                                                                                                                                                                                                                                                                     <  5:1627873/146‑1 (MQ=255)
                                                                                                                                      gcgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGGATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGTGCGTCACGCCCTGtt                                                                                                                                                                                                                                                                    <  7:330478/149‑1 (MQ=255)
                                                                                                                                      gcgcTGACTGGCATCTTCGCCCATATACCACGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCATAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                                                                                                                                                    <  7:1373192/149‑1 (MQ=255)
                                                                                                                                           gACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                                                                                                                                                                                               <  5:1323199/149‑1 (MQ=255)
                                                                                                                                           gACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                                                                                                                                                                                               <  7:1037263/149‑1 (MQ=255)
                                                                                                                                            aCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                                                                                                                                                                                              <  5:97726/149‑1 (MQ=255)
                                                                                                                                              tGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGca                                                                                                                                                                                                                                                            >  7:770913/1‑149 (MQ=255)
                                                                                                                                                   tCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaac                                                                                                                                                                                                                                                       <  5:1554609/149‑2 (MQ=255)
                                                                                                                                                   tCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                                                                                                                                                                                                       <  6:1472533/149‑1 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                                                                                                                                                                                                    >  6:1356218/1‑134 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                                                                                                                                                                                                    <  5:1356218/134‑1 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                                                                                                                                                <  7:826497/138‑1 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                                                                                                                                                >  8:826497/1‑138 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                                                                                                                                                  <  6:1263365/136‑1 (MQ=255)
                                                                                                                                                     ttCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                                                                                                                                                  >  5:1263365/1‑136 (MQ=255)
                                                                                                                                                       cGCCCATATACCATGTTACCTGCCCTACGGCTGGCAGTGAGACATCGCCTCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAATCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                                                                                                   <  8:1423753/149‑1 (MQ=255)
                                                                                                                                                        gCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                                                                                                                                                >  8:61223/1‑135 (MQ=255)
                                                                                                                                                        gCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                                                                                                                                                                                                  >  5:1564974/1‑149 (MQ=255)
                                                                                                                                                            atatACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGa                                                                                                                                                                                                                                              >  6:1459412/1‑149 (MQ=255)
                                                                                                                                                                aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                                                                                                                                                          >  5:1475907/1‑149 (MQ=255)
                                                                                                                                                                aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGt                                                                                                            <  2:166993/275‑1 (MQ=255)
                                                                                                                                                                aCCATGTTCCCTGCCCTACGGCTGGCAGGGAGACATCGCCACTAAATTGCATCATTTTTTGTTGCATTGTTTCGTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                                                                                                                                                          >  8:1625916/1‑149 (MQ=255)
                                                                                                                                                                 cnATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGAAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                                                                                                                                         >  7:520834/1‑149 (MQ=255)
                                                                                                                                                                 ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                                                                                                                                         <  7:641418/149‑1 (MQ=255)
                                                                                                                                                                   aTGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGc                                                                                                                                                                                                                                       >  5:1067806/1‑149 (MQ=255)
                                                                                                                                                                   aTGTTCCCTGCCCTACGGCTGGCAGTGAGACATAGCCACTCAATTGAATCATTTTTTGTTGCATCGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGAGCGTGACGCCCTGTTTTTATGCACAAAATGCCCCGGAAAGATGc                                                                                                                                                                                                                                       >  6:1006845/1‑149 (MQ=255)
                                                                                                                                                                    tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                                                                                                                                                                                                    >  2:284374/1‑183 (MQ=255)
                                                                                                                                                                       tCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTa                                                                                                                                                                                                                                   >  6:1382899/1‑149 (MQ=255)
                                                                                                                                                                       tCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATc                                                                                                                                                                                                    >  4:395964/1‑180 (MQ=255)
                                                                                                                                                                        cccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAt                                                                                                                                                                                                                                  <  6:1550804/149‑1 (MQ=255)
                                                                                                                                                                         ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                                                                                                                                                                 >  7:582969/1‑149 (MQ=255)
                                                                                                                                                                         ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACggg                                                                                                                                          >  2:255975/1‑236 (MQ=255)
                                                                                                                                                                         ccTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTCTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                                                                                                                                                                                                 >  6:1127457/1‑149 (MQ=255)
                                                                                                                                                                          cTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                                                                                                                                                                                                <  6:207781/149‑1 (MQ=255)
                                                                                                                                                                           tGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                                                                                                                                                                                                <  2:395968/180‑1 (MQ=255)
                                                                                                                                                                            gCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                                                                                                                                              >  8:1065991/1‑149 (MQ=255)
                                                                                                                                                                                tACGGCTGGCAGTGAAACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAttt                                                                                                                                                                                                                          >  6:293496/1‑149 (MQ=255)
                                                                                                                                                                                 aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                                                                                                                                                                                                         <  5:705623/149‑1 (MQ=255)
                                                                                                                                                                                  cGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                                                                                                                                                                                                       >  2:511051/1‑118 (MQ=255)
                                                                                                                                                                                   ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                                                                                                                                                                                                       <  7:1352078/149‑1 (MQ=255)
                                                                                                                                                                                    gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                                                                                                                                                      <  8:770913/149‑1 (MQ=255)
                                                                                                                                                                                    gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                                                                                                                                                      <  6:1684493/149‑1 (MQ=255)
                                                                                                                                                                                      tGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                                                                                                   <  4:511047/118‑1 (MQ=255)
                                                                                                                                                                                       ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGa                                                                                                                                                                                                                              <  3:255014/138‑1 (MQ=255)
                                                                                                                                                                                           gTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtca                                                                                                                                                       <  2:634720/205‑1 (MQ=255)
                                                                                                                                                                                            tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                                                                                                                                                                                                       >  5:384561/1‑140 (MQ=255)
                                                                                                                                                                                            tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                                                                                                                                                                                              >  5:217961/1‑149 (MQ=255)
                                                                                                                                                                                             gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTGTGCACAACATGCTCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCg                                                                                                                                                                                                             >  5:790921/1‑149 (MQ=255)
                                                                                                                                                                                                aCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTa                                                                                                                                                                                                          <  6:963526/149‑1 (MQ=255)
                                                                                                                                                                                                  aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                                                                                                   >  5:928485/1‑106 (MQ=255)
                                                                                                                                                                                                  aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                                                                                                                                                   <  6:928485/106‑1 (MQ=255)
                                                                                                                                                                                                       cACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                                                                                                                                                                                                   >  7:260853/1‑149 (MQ=255)
                                                                                                                                                                                                       cACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                                                                                                                                                                                                   <  7:241763/149‑1 (MQ=255)
                                                                                                                                                                                                        aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                                                                                                                                                                                                  <  8:1182818/149‑1 (MQ=255)
                                                                                                                                                                                                            aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                                                                                                                                                                                                       <  7:1127319/124‑1 (MQ=255)
                                                                                                                                                                                                             aTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTTATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                                                                                                                                                                                             >  6:509040/1‑149 (MQ=255)
                                                                                                                                                                                                              ttGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGt                                                                                                                                                                                            >  5:487360/1‑149 (MQ=255)
                                                                                                                                                                                                                    cATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                                                                                                                                                      <  8:935084/149‑1 (MQ=255)
                                                                                                                                                                                                                      ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                                                                                                                                                      <  5:1214826/115‑1 (MQ=255)
                                                                                                                                                                                                                      ttttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtt                                                                                                                                                                                                                                                                    >  5:565193/1‑69 (MQ=255)
                                                                                                                                                                                                                             ttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCATGTTTTTATGCACAAAATGccc                                                                                                                                                                                                                                                  <  5:1041904/80‑1 (MQ=255)
                                                                                                                                                                                                                                 aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                                                                                                                                              <  8:1529340/144‑1 (MQ=255)
                                                                                                                                                                                                                                 aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                                                                                                                                              >  7:1529340/1‑144 (MQ=255)
                                                                                                                                                                                                                                  ttGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATGTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCg                                                                                                                                                                        >  5:886829/1‑149 (MQ=255)
                                                                                                                                                                                                                                          tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAg                                                                                                                                                                                                                                           <  6:386260/74‑1 (MQ=255)
                                                                                                                                                                                                                                          tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCa                                                                                                                                                                >  8:403494/1‑149 (MQ=255)
                                                                                                                                                                                                                                          tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCa                                                                                                                                                                <  8:1327432/149‑1 (MQ=255)
                                                                                                                                                                                                                                          tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGt                                                                                                                             <  1:36692/184‑1 (MQ=255)
                                                                                                                                                                                                                                            ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                                                                                                                                              >  7:423043/1‑149 (MQ=255)
                                                                                                                                                                                                                                            ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                                                                                                                                              >  6:675298/1‑149 (MQ=255)
                                                                                                                                                                                                                                            ccAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTGTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGt                                                                                                                                                              >  8:677448/1‑149 (MQ=255)
                                                                                                                                                                                                                                             cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                                                                                                                                                                                                      >  5:386710/1‑92 (MQ=255)
                                                                                                                                                                                                                                              aGGTATTGCACTACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                                                                                                                                            >  7:958191/1‑149 (MQ=255)
                                                                                                                                                                                                                                              aGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTaa                                                                                                                                                            <  5:1453263/149‑1 (MQ=255)
                                                                                                                                                                                                                                               ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                                                                                                                                                                                                 >  7:717929/1‑111 (MQ=255)
                                                                                                                                                                                                                                               ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGa                         >  1:132901/1‑279 (MQ=255)
                                                                                                                                                                                                                                                gTATTGCACCAGCGTAAGGCAAAACAGAGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACt                                                                                                                                                          <  5:1006845/149‑1 (MQ=255)
                                                                                                                                                                                                                                                    tGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCANCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGNAAGTTGTAGCTGGCGCTCAGCCCAAGGTAAGGGCCCCAGCGGGCGNTNGAGTTATGGCCACGCAAAACGcgggggacgga                    >  3:79981/1‑269 (MQ=255)
                                                                                                                                                                                                                                                     gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatc                                                                                                                                                     <  6:76741/149‑1 (MQ=255)
                                                                                                                                                                                                                                                       accaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcag                                                                                                                                                   >  6:1230438/1‑149 (MQ=255)
                                                                                                                                                                                                                                                        ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAg                                                                                                     <  4:464811/194‑1 (MQ=255)
                                                                                                                                                                                                                                                           ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                                                                                                                                                                                                >  1:254244/1‑68 (MQ=255)
                                                                                                                                                                                                                                                            cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                                                                                                                                              >  6:1571018/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                    aaaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAg                                                                                <  4:262062/203‑1 (MQ=255)
                                                                                                                                                                                                                                                                      aaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                                                                                                                                                      >  7:1469984/1‑99 (MQ=255)
                                                                                                                                                                                                                                                                         aGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                                                                                                 >  5:180679/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                           ggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCg                                                                                                                               <  8:582969/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCgg                                                                                                                              >  7:184719/1‑149 (MQ=255)
                                                                                                                                                                                                                                                                            gCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCgg                                                                                                                              <  6:104557/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcaga                                                                                                                                                  <  7:1242782/125‑1 (MQ=255)
                                                                                                                                                                                                                                                                                gACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc                                                                                                                          <  5:738508/149‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                            
AGACCTTCTCTCGCAGACCGGTTAATGCTTCCCCAACCACCTTTTCGGCACCGCCATCGGCATACATTTCGGCGGTATCAATGAGGGTTAAACCGAGTTCAATGCCCGCGCGTAGTGCAGCAACTTCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGCTCCAGGTAAGGGCTCCAGCTGTCGTTCGGGTTATAGCCACGCAGACCGCTGCGAGCGGACTCTTTGCGCGATACGCC  >  NC_000913/1865411‑1865950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: