BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,985,112	C→G	10.2%	intergenic (‑43/+27)	araG ← / ← araF	L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,985,112	0	C	G	10.2%	869.1 / 75.4	256	intergenic (‑43/+27)	araG/araF	L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base C (120/108); new base G (17/9); total (138/118)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.29e-02

Only 100 of 268 total aligned reads displayed.

GTGTCGGAAAAGGACATTTCCTGCCCATTAATCACTACAGAACCCGTGGTTGGCGCATAGTTGCCGCTGAGGATTTTTAAGAGAGTTGATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTTATAGCCATGTACGTCCGGGCTTGGCAGCAGGGAACCGTAGAAGCCGGTTGCCTGTGCTTTAGACAGTTCGCTCACCGCATCCACACCGTTAATGCCAATGC  >  NC_000913/1984843‑1985377

                                        aaCCCGTGGTTGGCGCATAGTTGCCGCTGAGGATTTTTAAGAGAGTTGATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGACTTAACGCCGGGAACCGTTTTACCGATGCCGCGACATGAGAGATCAGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCAAACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAATTCTTTCCCCCTAAACCt                                                                                                                                                                                                                              >  4:510692/1‑275 (MQ=255)

                                                               ccGCTGAGGATTTTTAAGAGAGTTGATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCCTGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCctttc                                                                                                                                                                                                        >  2:36871/1‑272 (MQ=255)

                                                                       gATTTTTAAGAGAGTTTATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgc                                                                                                                                                                                             <  1:1744/277‑1 (MQ=255)

                                                                            ttAAGAGAGTTGATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTg                                                                                                                                                                                          <  2:259692/275‑1 (MQ=255)

                                                                                               tGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCAGGGAAACGTTTTCCCGATTCCGCGAAATGAGAGATACGGGGTAGACTGTAGCTTCACGACTCCGTGACTCAATGGTCGTTCACACCCCCTCTGCATAATGCAGAGGGGTATTTTTCCGGCCATTTACTTACCCACTAAACCTTTTTTCTACAGTTTTTCTTTAAAGTTTTAACGCGTGATCAGTActggtccatta                                                                                                                                                                        >  4:332349/1‑264 (MQ=255)

                                                                                                   cccttttCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgc                                                                                                                                                                                             <  2:332353/246‑1 (MQ=255)

                                                                                                           aCCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGCGGGGAATTTTTCCGGCAACTTACTTACCGCCTAACCCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtt                                                                                                                                                           >  4:235850/1‑275 (MQ=255)

                                                                                                                           aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatcat                                                                                                                                                                                                                                                                         <  8:1527945/149‑1 (MQ=255)

                                                                                                                           aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatcat                                                                                                                                                                                                                                                                         >  7:91014/1‑149 (MQ=255)

                                                                                                                                aCCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCaga                                                                                                                                                                                                                                                                    <  8:693250/149‑1 (MQ=255)

                                                                                                                                 ccGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCagag                                                                                                                                                                                                                                                                   <  5:572305/149‑1 (MQ=255)

                                                                                                                                  cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAgg                                                                                                                                                                                                                                                                  <  5:431641/149‑1 (MQ=255)

                                                                                                                                  cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAgg                                                                                                                                                                                                                                                                  <  5:1176174/149‑1 (MQ=255)

                                                                                                                                   ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                                                                                                                                                 <  8:91014/149‑1 (MQ=255)

                                                                                                                                   ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                                                                                                                                                 <  5:1289173/149‑1 (MQ=255)

                                                                                                                                     cATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGc                                                                                                                                                                                                                                                    <  2:382496/160‑1 (MQ=255)

                                                                                                                                             tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTcc                                                                                                                                                                                                                                                       >  5:548141/1‑149 (MQ=255)

                                                                                                                                               aaaaCTAATATCCGTCAGCGCCTTGACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                                                                                                                                        <  4:105198/210‑1 (MQ=255)

                                                                                                                                               aaaaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCgg                                                                                                                                                                                                                                                     >  6:648456/1‑149 (MQ=255)

                                                                                                                                               aaaaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCgg                                                                                                                                                                                                                                                     >  6:1258296/1‑149 (MQ=255)

                                                                                                                                                 aaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCa                                                                                                                                                                                                                                                   >  8:1078840/1‑149 (MQ=255)

                                                                                                                                                   cTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCaaa                                                                                                                                                                                                                                                 >  7:267966/1‑149 (MQ=255)

                                                                                                                                                    tAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAt                                                                                                                                                                                                                                                >  8:1686710/1‑149 (MQ=255)

                                                                                                                                                      atatCCGTCAGCGCCTTAACGCCGGGAAACGTTTTNCCGATGNCGCNAAATGAGNGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                                                                                                                                                                                                                     <  3:37350/174‑1 (MQ=255)

                                                                                                                                                      atatCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                                                                                                                                                                                                                     >  1:37350/1‑174 (MQ=255)

                                                                                                                                                       tatCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCAttt                                                                                                                 >  2:85919/1‑273 (MQ=255)

                                                                                                                                                            gTCAGCGGCTTAAAGCCGGGAAACGTTTTCACGATGCCGCGAAATGAGGGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCGCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAGTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCt                                                                                                             <  4:349769/272‑1 (MQ=255)

                                                                                                                                                             tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCg                                                                                                                                                                                                                                       >  8:1385104/1‑149 (MQ=255)

                                                                                                                                                             tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGGAAACAGTAACACGTCGGTAACTTCGGTAAATTTTGGAGGTTCAACCTCTTTTGCTTCCCAGTTGGAAAGAATTTCGCGGGAt                                                                                                         >  4:662145/1‑275 (MQ=255)

                                                                                                                                                              cAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgcg                                                                                                                                                                                            >  1:153659/1‑191 (MQ=255)

                                                                                                                                                                gcgcCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTta                                                                                                      <  2:133054/275‑1 (MQ=255)

                                                                                                                                                                   ccTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTTATAg                                                                                                   <  2:536653/275‑1 (MQ=255)

                                                                                                                                                                         cGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                                                                                                                                           <  7:1004857/149‑1 (MQ=255)

                                                                                                                                                                              ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCTGTGAATCACTGGTCGTTCACACTCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCCCCACTTTTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGCTGGTAACTTCGGTAATTTTTGGCGGTTCAACGTTTCTTGCTACCCAGTTGAAAACAATTTACCTGGATTTATAGTCATGGACGTc                                                                                        >  4:409/1‑275 (MQ=255)

                                                                                                                                                                              ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                                                                                                                                      >  5:966779/1‑149 (MQ=255)

                                                                                                                                                                              ggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTAGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                                                                                                                                      <  5:1078912/149‑1 (MQ=255)

                                                                                                                                                                               gAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTcc                                                                                                                                                                                                                     >  6:211471/1‑149 (MQ=255)

                                                                                                                                                                                aaaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAgg                                                                                                                                                                                                                                                                  <  6:398170/103‑1 (MQ=255)

                                                                                                                                                                                  aCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTgg                                                                                                                                                       <  4:79558/208‑1 (MQ=255)

                                                                                                                                                                                    gTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttc                                                                                                                                                                                                                >  8:310034/1‑149 (MQ=255)

                                                                                                                                                                                     ttttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttct                                                                                                                                                                                                               <  7:226038/149‑1 (MQ=255)

                                                                                                                                                                                      tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttctt                                                                                                                                                                                                              <  7:1466461/149‑1 (MQ=255)

                                                                                                                                                                                             aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                                                                                                                                       <  7:1129483/149‑1 (MQ=255)

                                                                                                                                                                                             aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                                                                                                                                       <  5:608961/149‑1 (MQ=255)

                                                                                                                                                                                                        tGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTCTCAcgcg                                                                                                                                                                                            >  7:135742/1‑149 (MQ=255)

                                                                                                                                                                                                            agaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                                                                                                                                        <  7:407817/149‑1 (MQ=255)

                                                                                                                                                                                                            agaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGAt                                                                                                                                                                                        <  5:1202097/149‑1 (MQ=255)

                                                                                                                                                                                                              aTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      <  7:1078840/149‑1 (MQ=255)

                                                                                                                                                                                                              aTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      >  8:1646519/1‑149 (MQ=255)

                                                                                                                                                                                                              aTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      <  7:740547/149‑1 (MQ=255)

                                                                                                                                                                                                              aTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      >  8:1584738/1‑149 (MQ=255)

                                                                                                                                                                                                              aTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTACTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      >  7:1153495/1‑149 (MQ=255)

                                                                                                                                                                                                                   gggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTAcc                                                                                                                                                                                 <  7:352362/149‑1 (MQ=255)

                                                                                                                                                                                                                        gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCttt                                                                                                                                                                                                                            <  8:1458752/101‑1 (MQ=255)

                                                                                                                                                                                                                        gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCa                                                                                                                                                                                      <  7:1660784/139‑1 (MQ=255)

                                                                                                                                                                                                                                  tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCg                                                                                                                                                                  >  5:653043/1‑149 (MQ=255)

                                                                                                                                                                                                                                  tAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCg                                                                                                                                                                  >  6:530370/1‑149 (MQ=255)

                                                                                                                                                                                                                                      gTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttac                                                                                                                                                                                                                                             <  7:810213/70‑1 (MQ=255)

                                                                                                                                                                                                                                        ctcCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAt                                                                                                                                                            >  6:874831/1‑149 (MQ=255)

                                                                                                                                                                                                                                         tcCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTc                                                                                                                                                                   >  5:1336616/1‑141 (MQ=255)

                                                                                                                                                                                                                                         tcCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtt                                                                                                                                                           >  7:649055/1‑149 (MQ=255)

                                                                                                                                                                                                                                         tcCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtt                                                                                                                                                           >  8:113856/1‑149 (MQ=255)

                                                                                                                                                                                                                                          ccGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTTATAGCCATGTACGTCCGGGCTTGGCAGCAGGGAACCGTAGAAGCCGGTTGCCTGTGCTTTAGACAGTTCGCTCAc                            <  2:616510/275‑1 (MQ=255)

                                                                                                                                                                                                                                           cGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGt                                                                                                                                                                         >  6:344543/1‑133 (MQ=255)

                                                                                                                                                                                                                                           cGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGt                                                                                                                                                                         <  5:344543/133‑1 (MQ=255)

                                                                                                                                                                                                                                           cGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAAtttt                                                                                                                                                         >  5:1457682/1‑149 (MQ=255)

                                                                                                                                                                                                                                             tGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTgg                                                                                                                                                       <  8:639185/149‑1 (MQ=255)

                                                                                                                                                                                                                                             tGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTgg                                                                                                                                                       >  7:144777/1‑149 (MQ=255)

                                                                                                                                                                                                                                             tGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTgg                                                                                                                                                       <  6:548141/149‑1 (MQ=255)

                                                                                                                                                                                                                                               aaTCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTta                                                                                                      <  1:76828/196‑1 (MQ=255)

                                                                                                                                                                                                                                                     tGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                                                                                                                                           <  8:377020/73‑1 (MQ=255)

                                                                                                                                                                                                                                                      ggTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                                                                                                                                              <  5:275979/149‑1 (MQ=255)

                                                                                                                                                                                                                                                      ggTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                                                                                                                                              <  8:144777/149‑1 (MQ=255)

                                                                                                                                                                                                                                                         cGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTTATAGCCATGTACGTCCGGGCTTGGCAGCAGGGAACCGTAGAAGCCGGTTGCCTGTGCTTTAGACAGTTCGCTCACCGCATCCACACCGtt             <  2:235853/275‑1 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCg                                                                                                                                                                                                                                       >  8:5422/1‑56 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCg                                                                                                                                                                                                                                       <  7:5422/56‑1 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          >  7:1622919/1‑149 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          >  5:702285/1‑149 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          <  7:571844/149‑1 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          >  5:800357/1‑149 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          <  5:874831/149‑1 (MQ=255)

                                                                                                                                                                                                                                                          gTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCt                                                                                                                                          >  8:101692/1‑149 (MQ=255)

                                                                                                                                                                                                                                                             cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCa                                                                                                                                                                                                <  2:497733/92‑1 (MQ=255)

                                                                                                                                                                                                                                                             cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCATGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtttt                                                                                                                                       <  5:1615079/149‑1 (MQ=255)

                                                                                                                                                                                                                                                             cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtttt                                                                                                                                       <  6:966779/149‑1 (MQ=255)

                                                                                                                                                                                                                                                              acacCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTg                                                                                                                                      >  5:1150435/1‑149 (MQ=255)

                                                                                                                                                                                                                                                               cacCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGc                                                                                                                                     >  5:1539505/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                ttccccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                                                                                                                                              >  6:1309455/3‑139 (MQ=255)

                                                                                                                                                                                                                                                                acCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCt                                                                                                                                    >  8:40553/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                  ccccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAc                                                                                                                                  >  7:1232535/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                  ccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCgg                                                                                                                                                                 >  6:644409/1‑118 (MQ=255)

                                                                                                                                                                                                                                                                  ccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                                                                                                                                              >  5:394290/1‑137 (MQ=255)

                                                                                                                                                                                                                                                                  ccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAc                                                                                                                                  <  6:1498057/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                  ccccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAc                                                                                                                                  >  7:1679072/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                   cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc                                                                                                                                 >  8:1153860/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                    ccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc                                                                                                                                >  5:471079/1‑149 (MQ=255)

                                                                                                                                                                                                                                                                       ctGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGt                                                                                                                             <  6:1539505/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                        tGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGtt                                                                                                                            <  8:1211559/149‑1 (MQ=255)

                                                                                                                                                                                                                                                                            tcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTATGCTACCCAGTTGTAAAGCATTTc                                                                                                                >  2:456084/1‑157 (MQ=255)

                                                                                                                                                                                                                                                                            tcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTCCTTCTTTAAACTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTaa                                                                                                                        >  6:1689797/1‑149 (MQ=255)

GTGTCGGAAAAGGACATTTCCTGCCCATTAATCACTACAGAACCCGTGGTTGGCGCATAGTTGCCGCTGAGGATTTTTAAGAGAGTTGATTTTCCTGCGCCATTTTCACCCATCAACGCATGAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAGCATTTCGCTGGATTTATAGCCATGTACGTCCGGGCTTGGCAGCAGGGAACCGTAGAAGCCGGTTGCCTGTGCTTTAGACAGTTCGCTCACCGCATCCACACCGTTAATGCCAATGC  >  NC_000913/1984843‑1985377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`