Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 958,771 A→T 9.3% intergenic (+30/‑41) serC → / → aroA 3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913958,7710AT 9.3% 752.1 / 48.7 227intergenic (+30/‑41)serC/aroA3‑phosphoserine/phosphohydroxythreonine aminotransferase/5‑enolpyruvylshikimate‑3‑phosphate synthetase
Reads supporting (aligned to +/- strand):  ref base A (104/101);  new base T (8/13);  total (112/115)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 232 total aligned reads displayed.

ACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACAC  >  NC_000913/958624‑958919
                                                                                                                                                   |                                                                                                                                                    
  tGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACagccagcc                                                                                                                                                   >  3:1477415/1‑149 (MQ=255)
      aGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgtg                                                                                                                                               <  2:616097/149‑1 (MQ=255)
        gTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTggg                                                                                                                                             <  1:969277/149‑1 (MQ=255)
          cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGTAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggga                                                                                                                                           <  4:785466/149‑2 (MQ=255)
          cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggga                                                                                                                                           <  3:1554390/149‑2 (MQ=255)
          cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTgggga                                                                                                                                           <  3:750032/149‑2 (MQ=255)
          cACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTAGCTGTgggga                                                                                                                                           <  3:106440/149‑2 (MQ=255)
           aCCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtt                                                                                                                                          >  2:876132/1‑149 (MQ=255)
           aCCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtt                                                                                                                                          <  3:323125/149‑1 (MQ=255)
            ccGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttt                                                                                                                                         >  3:483144/1‑149 (MQ=255)
             cGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGGCTGGCTGTGGGGatta                                                                                                                                        <  3:1362377/149‑2 (MQ=255)
              gtgtGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGttttt                                                                                                                                       <  3:1155878/149‑1 (MQ=255)
                 tgGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAtt                                                                                                                                    <  1:660378/149‑1 (MQ=255)
                   gTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTa                                                                                                                                      >  2:1672948/1‑145 (MQ=255)
                   gTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTa                                                                                                                                      <  1:1672948/145‑1 (MQ=255)
                          gAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTa                                                                                                                           <  2:1345453/149‑1 (MQ=255)
                              gcgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                       <  4:1477415/149‑1 (MQ=255)
                              gcgcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTagaga                                                                                                                       <  2:1375881/149‑1 (MQ=255)
                                gcgcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagt                                                                                                                     >  3:954187/1‑149 (MQ=255)
                                  gcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttg                                                                                                                   >  3:516174/1‑149 (MQ=255)
                                  gcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttg                                                                                                                   >  3:880588/1‑149 (MQ=255)
                                  gcTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttg                                                                                                                   >  1:768017/1‑149 (MQ=255)
                                       tATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTc                                                                                                              >  2:413801/1‑149 (MQ=255)
                                         tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCCCAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                   <  2:1252364/126‑1 (MQ=255)
                                         tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttgag                                                                                                                 <  1:879406/144‑1 (MQ=255)
                                         tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagtt                                                                                                                    >  2:1394159/1‑141 (MQ=255)
                                         tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                            <  2:1197231/149‑1 (MQ=255)
                                         tttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTCCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCGCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                   >  1:1252364/1‑126 (MQ=255)
                                         tttATAACGCCATGCCGCCGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCAt                                                                                                            <  2:1037015/149‑1 (MQ=255)
                                          ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                                                                           <  1:1014449/149‑1 (MQ=255)
                                          ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                                                                           <  3:1507252/149‑1 (MQ=255)
                                          ttATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATg                                                                                                           <  3:88783/149‑1 (MQ=255)
                                           tataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATgg                                                                                                          <  4:483144/149‑1 (MQ=255)
                                            ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                                                                                         >  4:826360/1‑149 (MQ=255)
                                            ataACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGa                                                                                                         >  3:285719/1‑149 (MQ=255)
                                              aaCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                                                                              >  2:674386/1‑126 (MQ=255)
                                               aCGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATc                                                                                                      >  1:1293321/1‑149 (MQ=255)
                                                  ccATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCt                                                                                                   >  4:1541837/1‑149 (MQ=255)
                                                    aTGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGa                                                                                                 <  4:635542/149‑1 (MQ=255)
                                                      gCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACg                                                                                               >  2:902941/1‑149 (MQ=255)
                                                               aGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCtgtg                                                                                                                                               <  2:1708523/92‑1 (MQ=255)
                                                                ggCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGtt                                                                                             >  2:898048/1‑141 (MQ=255)
                                                                 gCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCAt                                                                                    >  4:1609203/1‑149 (MQ=255)
                                                                  cGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATc                                                                                   <  1:1414061/149‑1 (MQ=255)
                                                                   gTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCg                                                                                  >  1:566554/1‑149 (MQ=255)
                                                                        aGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCgt                                                                             <  1:1431035/149‑1 (MQ=255)
                                                                              gACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACCACCCATCGCTCGTGTCGAt                                                                       >  1:840252/1‑149 (MQ=255)
                                                                              gACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGAt                                                                       >  1:1640885/1‑149 (MQ=255)
                                                                               aCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                                                                              >  1:1338276/1‑93 (MQ=255)
                                                                               aCAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgtt                                                                                                                              <  2:1338276/93‑1 (MQ=255)
                                                                               aCAGACTTCATGGCTGAGTTCGTACGCCGTCACGGTTAATGCCGGAATGTTGCTTCATCTCCACAGCCAGAATGCGGGGAATTTATTTCTGTTGTAGAGAGTTTATTTCATGGATTCCCTGAAGTTACAACCCATCGCTCGTGTCGATg                                                                      >  4:175459/1‑149 (MQ=255)
                                                                                cAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATgg                                                                     <  4:919493/149‑1 (MQ=255)
                                                                                 aGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGc                                                                    >  2:332449/1‑149 (MQ=255)
                                                                                     ttCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTa                                                                <  3:373054/149‑1 (MQ=255)
                                                                                      tCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTAt                                                               <  4:380197/149‑1 (MQ=255)
                                                                                      tCATGGTGGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTAt                                                               >  2:914985/1‑149 (MQ=255)
                                                                                         tGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                   <  1:1503509/78‑1 (MQ=255)
                                                                                         tGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTAttt                                                                                                                                   >  2:1503509/1‑78 (MQ=255)
                                                                                           gTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACaa                                                                                         >  2:77264/1‑118 (MQ=255)
                                                                                           gTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATc                                                          >  4:1357557/1‑149 (MQ=255)
                                                                                               aGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGcc                                                      <  1:1161814/149‑1 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtttt                                                                                                                                        <  2:225220/66‑1 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGtttt                                                                                                                                        >  1:225220/1‑66 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACaa                                                                                         >  3:1181900/1‑113 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACaa                                                                                         <  4:1181900/113‑1 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGccc                                                     >  1:1387040/1‑149 (MQ=255)
                                                                                                gTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGccc                                                     <  3:175459/149‑1 (MQ=255)
                                                                                                 ttCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgt                                                                                                                               <  1:1111868/74‑1 (MQ=255)
                                                                                                 ttCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCtgt                                                                                                                               >  2:1111868/1‑74 (MQ=255)
                                                                                                    gAACGCTGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGc                                                       >  4:512761/1‑143 (MQ=255)
                                                                                                    gAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGtt                                                 >  1:1269746/1‑149 (MQ=255)
                                                                                                     aaCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTc                                                >  3:997888/1‑149 (MQ=255)
                                                                                                      aCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTcc                                               >  3:1246856/1‑149 (MQ=255)
                                                                                                      aCGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTcc                                               <  4:199503/149‑1 (MQ=255)
                                                                                                        gCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCaa                                             <  2:154665/149‑1 (MQ=255)
                                                                                                          cGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAaga                                           >  1:350521/1‑149 (MQ=255)
                                                                                                            tCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGc                                         >  3:8110/1‑149 (MQ=255)
                                                                                                                    aaTGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAAc                                <  3:161964/149‑1 (MQ=255)
                                                                                                                         cGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAgagttga                                                                                                                  >  2:706858/1‑63 (MQ=255)
                                                                                                                         cGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACcgcg                            >  3:131697/1‑149 (MQ=255)
                                                                                                                         cGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACcgcg                            >  4:279192/1‑149 (MQ=255)
                                                                                                                          gAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCgcgc                           >  1:902002/1‑149 (MQ=255)
                                                                                                                          gAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCgcgc                           <  4:15147/149‑1 (MQ=255)
                                                                                                                           aaaTTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTc                                                                          <  1:191434/101‑1 (MQ=255)
                                                                                                                              ttttGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGGTTCTAACCGCGCTTTa                       >  3:1602401/1‑149 (MQ=255)
                                                                                                                                  gCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGc                   <  2:116687/149‑1 (MQ=255)
                                                                                                                                   cTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCt                  <  4:409834/149‑1 (MQ=255)
                                                                                                                                    ttAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTg                 >  1:495655/1‑149 (MQ=255)
                                                                                                                                     tAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTgg                >  1:263843/1‑149 (MQ=255)
                                                                                                                                     tAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTgg                <  4:1414323/149‑1 (MQ=255)
                                                                                                                                     tAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTgg                <  2:495655/149‑1 (MQ=255)
                                                                                                                                       taccccACAGGCTGGCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcg              >  4:1093459/3‑149 (MQ=255)
                                                                                                                                         ccccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTggcggc            >  2:1633615/1‑149 (MQ=255)
                                                                                                                                          cccACAGGCTGGCTGTGGGGCTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCa           >  4:527790/1‑149 (MQ=255)
                                                                                                                                          cccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCa           >  4:1241548/1‑149 (MQ=255)
                                                                                                                                           ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt          <  2:263843/149‑1 (MQ=255)
                                                                                                                                           ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt          >  1:311794/1‑149 (MQ=255)
                                                                                                                                           ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt          <  4:551953/149‑1 (MQ=255)
                                                                                                                                           ccACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCAt          >  4:65271/1‑149 (MQ=255)
                                                                                                                                             acaGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTa        <  1:698350/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACTGAAAGGTCACCGTGTGGTCGGCGGAATGCGCGCTTCTATTTATAACGCCATGCCGCTGGAAGGCGTTAAAGCGCTGACAGACTTCATGGTTGAGTTCGAACGCCGTCACGGTTAATGCCGAAATTTTGCTTAATCCCCACAGCCAGCCTGTGGGGTTTTTATTTCTGTTGTAGAGAGTTGAGTTCATGGAATCCCTGACGTTACAACCCATCGCTCGTGTCGATGGCACTATTAATCTGCCCGGTTCCAAGAGCGTTTCTAACCGCGCTTTATTGCTGGCGGCATTAGCACAC  >  NC_000913/958624‑958919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: