Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,141,148 C→T 6.8% intergenic (+162/+34) flgL → / ← rne flagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,141,1480CT 6.8% 619.1 / 24.0 192intergenic (+162/+34)flgL/rneflagellar hook‑filament junction protein/endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
Reads supporting (aligned to +/- strand):  ref base C (81/98);  new base T (3/10);  total (84/108)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.39e-01

Only 100 of 194 total aligned reads displayed.

TCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGA  >  NC_000913/1141004‑1141296
                                                                                                                                                |                                                                                                                                                    
tCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGtt                                                                                                                                                  >  2:1090763/1‑149 (MQ=255)
  aTGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAc                                                                                                                                                >  4:1181885/1‑149 (MQ=255)
   tGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTAcc                                                                                                                                               >  4:926418/1‑149 (MQ=255)
    gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              <  2:1339600/149‑1 (MQ=255)
    gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              <  1:128025/149‑1 (MQ=255)
    gAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCa                                                                                                                                              <  3:175602/149‑1 (MQ=255)
      aaCTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAgg                                                                                                                                            <  2:916090/149‑1 (MQ=255)
        cTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGc                                                                                                                                          <  3:383373/149‑1 (MQ=255)
         tGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCt                                                                                                                                         <  2:861738/149‑1 (MQ=255)
           ggTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCttt                                                                                                                                       <  2:522313/149‑2 (MQ=255)
            gTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCtttt                                                                                                                                      <  4:425993/149‑3 (MQ=255)
             tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                     >  3:71558/1‑149 (MQ=255)
             tATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAt                                                                                                                                     >  3:701799/1‑149 (MQ=255)
              aTGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAtt                                                                                                                                    >  3:1230708/1‑149 (MQ=255)
              aTGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGAtt                                                                                                                                    <  1:1646183/149‑1 (MQ=255)
                 ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                 >  3:875865/1‑149 (MQ=255)
                 ttttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACt                                                                                                                                 >  2:679850/1‑149 (MQ=255)
                   ttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACttt                                                                                                                               <  2:968765/149‑1 (MQ=255)
                   ttGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACttt                                                                                                                               <  3:1023843/149‑1 (MQ=255)
                    tGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTg                                                                                                                              <  2:73734/149‑1 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             <  1:1121541/149‑1 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             >  2:486446/1‑149 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             <  3:794478/149‑1 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             <  4:710346/149‑1 (MQ=255)
                     gTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             >  4:1357226/1‑149 (MQ=255)
                          cctgcTCTGGGATCGCTGGGGCGGGCATTGTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTaa                                                                                                                        >  3:1007033/1‑149 (MQ=255)
                           ctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCATGGCTTGATTACTTTGAGCTAat                                                                                                                       <  4:456737/149‑1 (MQ=255)
                           ctgcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAat                                                                                                                       >  3:395140/1‑149 (MQ=255)
                             gcTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAatta                                                                                                                     <  3:1181885/149‑1 (MQ=255)
                              ctctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattat                                                                                                                    <  2:1322529/149‑1 (MQ=255)
                               tctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAattatt                                                                                                                   <  4:1237520/149‑1 (MQ=255)
                                ctGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAAttatta                                                                                                                  <  1:992566/149‑1 (MQ=255)
                                   ggATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTc                                                                                                               >  3:776645/1‑149 (MQ=255)
                                     aTCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGa                                                                                                                                      <  3:1622600/124‑1 (MQ=255)
                                     aTCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCaa                                                                                                             <  4:1169880/149‑1 (MQ=255)
                                      tCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAAc                                                                                                            <  2:1379351/149‑1 (MQ=255)
                                       cGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACa                                                                                                           <  1:1210674/149‑1 (MQ=255)
                                        gCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAg                                                                                                          <  1:367810/149‑1 (MQ=255)
                                         cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             >  2:1417038/1‑129 (MQ=255)
                                         cTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGa                                                                                                                             <  1:1417038/129‑1 (MQ=255)
                                             ggcgggcATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGc                                                                                                     <  1:1322198/149‑1 (MQ=255)
                                                     tttttttGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACgcgc                                                                                             <  2:618311/149‑1 (MQ=255)
                                                      ttttttGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCa                                                                                            <  4:1573644/149‑1 (MQ=255)
                                                               tATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCa                                                                                   >  3:325371/1‑149 (MQ=255)
                                                                 ttttGTATAGCTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCATAGATGAAATAAAAAAGCCCTGGCAGTTACTAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCaga                                                                                 >  2:361772/1‑149 (MQ=255)
                                                                      cATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCat                                                                            <  1:486446/149‑1 (MQ=255)
                                                                       aTTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCatg                                                                           <  4:840203/149‑1 (MQ=255)
                                                                        ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCgg                                                                                     <  2:204588/138‑1 (MQ=255)
                                                                        ttgttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCgg                                                                                     >  1:204588/1‑138 (MQ=255)
                                                                           ttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCttt                                                                                                                                       >  4:507902/1‑84 (MQ=38)
                                                                           ttgGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGTAACTGCCAGGGCttt                                                                                                                                       <  3:507902/85‑2 (MQ=38)
                                                                             ggTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATgtgt                                                                     <  4:395140/149‑1 (MQ=255)
                                                                                tAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGc                                                                  <  1:530590/149‑1 (MQ=255)
                                                                                   cAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACt                                                                                                                >  1:225419/1‑100 (MQ=255)
                                                                                   cAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGAATGATGTGTTGCCGt                                                               <  4:1245642/149‑1 (MQ=255)
                                                                                    aaGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGTAGCGGCAGAGGCATGATGTGTTGCCGTa                                                              <  3:111743/149‑1 (MQ=255)
                                                                                    aaGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTa                                                              >  2:1213808/1‑149 (MQ=255)
                                                                                       gATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGa                                                           <  1:1476104/149‑1 (MQ=255)
                                                                                            cATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAatt                                                                                                                      <  3:220653/85‑1 (MQ=255)
                                                                                            cATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAatt                                                                                                                      >  4:220653/1‑85 (MQ=255)
                                                                                            cATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGAc                                                                                                 >  1:314716/1‑106 (MQ=255)
                                                                                            cATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGaccacc                                                      >  4:26644/1‑149 (MQ=255)
                                                                                              ttCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCt                                                                                                                                         >  1:742221/1‑64 (MQ=255)
                                                                                              ttCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGAtgtg                                                                      >  1:621657/1‑131 (MQ=255)
                                                                                              ttCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTg                                                    >  3:89285/1‑149 (MQ=255)
                                                                                                 gATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCgg                                                 <  2:556349/149‑1 (MQ=255)
                                                                                                  aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGtt                                                                                                       >  2:1047072/1‑94 (MQ=255)
                                                                                                  aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGtt                                                                                                       <  1:1047072/94‑1 (MQ=255)
                                                                                                  aTGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGc                                                >  4:179125/1‑149 (MQ=255)
                                                                                                   tGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGc                                                                              >  2:175920/1‑118 (MQ=255)
                                                                                                   tGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGc                                                                              <  1:175920/118‑1 (MQ=255)
                                                                                                   tGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCa                                               >  3:999099/1‑149 (MQ=255)
                                                                                                   tGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCa                                               >  4:588233/1‑149 (MQ=255)
                                                                                                      aTTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCt                                            >  1:857303/1‑149 (MQ=255)
                                                                                                       ttttAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCtt                                           >  1:1054000/1‑149 (MQ=255)
                                                                                                       ttttAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCtt                                           >  2:959643/1‑149 (MQ=255)
                                                                                                          tAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTAc                                        <  2:315621/149‑1 (MQ=255)
                                                                                                           aaTATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTAcc                                       <  4:1224708/149‑1 (MQ=255)
                                                                                                               tGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACttt                                                                                                                               <  1:43103/57‑1 (MQ=255)
                                                                                                               tGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACttt                                                                                                                               >  2:43103/1‑57 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                 <  3:269805/149‑1 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                 <  1:361772/149‑1 (MQ=255)
                                                                                                                 ttGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGaa                                 >  2:986055/1‑149 (MQ=255)
                                                                                                                        aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                          <  2:857303/149‑1 (MQ=255)
                                                                                                                        aaaGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCaaaa                          <  3:399072/149‑1 (MQ=255)
                                                                                                                           gATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTa                       <  2:1599109/149‑1 (MQ=255)
                                                                                                                              gAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAggg                    <  3:1240019/149‑1 (MQ=255)
                                                                                                                               aaaTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATCACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGgc                   <  3:1099471/149‑1 (MQ=255)
                                                                                                                                aaTAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGgcg                  >  4:6780/1‑149 (MQ=255)
                                                                                                                                  tAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCt                >  4:962019/1‑149 (MQ=255)
                                                                                                                                     aaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAg                                <  3:1105906/130‑1 (MQ=255)
                                                                                                                                     aaaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAg                                >  4:1105906/1‑130 (MQ=255)
                                                                                                                                      aaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCa            <  2:241169/149‑1 (MQ=255)
                                                                                                                                      aaaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCa            <  1:337822/149‑1 (MQ=255)
                                                                                                                                       aaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGac                                                          >  2:1632392/1‑102 (MQ=255)
                                                                                                                                       aaGCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGac                                                          <  1:1632392/102‑1 (MQ=255)
                                                                                                                                       aaGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAg           >  1:7709/1‑149 (MQ=255)
                                                                                                                                         gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTa                                                              <  3:1440695/96‑1 (MQ=255)
                                                                                                                                         gCCCTGGTAACTGCCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTc         >  1:1439818/1‑149 (MQ=255)
                                                                                                                                                cAGTTACCACGGCTTGATTACTTTGAGCTAATTATTACTCTTTAGGTTGCGGACGCGCAGGATCGGCAGAGGCATGATGTGTTGTCGTATGACCACCTCCGGTACCTTTACCTTCGAAGGCAAAAGTAGCGCGCTGCCAGTTACtgtgg  <  4:746099/149‑2 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TCATGAAACTGGGTATGTTTTGTCTGCCTGCTCTGGGATCGCTGGGGCGGGCATTTTTTTGCCTATTTTGCATTGTTGGTTAGCAAGGATGCCATTCGATGAATTTTAATATGTTGATTCAAAGATGAAATAAAAAAGCCCTGGCAGTTACCAGGGCTTGATTACTTTGAGCTAATTATTACTCAACAGGTTGCGGACGCGCAGGAGCGGCAGAGGCATGATGTGTTGCCGTATGACCACCTGCGGCACCTTTACCTTCGAAGGCAAAAGTAGGGCGCTGCCAGTCACTGTGA  >  NC_000913/1141004‑1141296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: