Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,426,422 G→A 6.3% intergenic (+340/‑32) ydaY → / → tmpR pseudogene, Rac prophage;Phage or Prophage Related/Rac prophage; pseudogene, tail protein family;Phage or Prophage Related; putative alpha helix protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,426,4220GA 6.3% 670.2 / 21.2 204intergenic (+340/‑32)ydaY/tmpRpseudogene, Rac prophage;Phage or Prophage Related/Rac prophage; pseudogene, tail protein family;Phage or Prophage Related; putative alpha helix protein
Reads supporting (aligned to +/- strand):  ref base G (86/105);  new base A (5/8);  total (91/113)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 206 total aligned reads displayed.

GAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGC  >  NC_000913/1426274‑1426566
                                                                                                                                                    |                                                                                                                                                
gAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTcgcg                                                                                                                                                  >  1:192111/1‑149 (MQ=255)
 aGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCgg                                                                                                                                                 >  4:1064776/1‑149 (MQ=255)
    aGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTgg                                                                                                                                              <  3:759294/149‑1 (MQ=255)
    aGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTgg                                                                                                                                              <  2:1618708/149‑1 (MQ=255)
       gtgtgtAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACCGCGAAGTGGGtt                                                                                                                                           <  3:117881/149‑1 (MQ=255)
        tgtgtAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGttt                                                                                                                                          >  3:1313199/1‑149 (MQ=255)
          tgtAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGttttt                                                                                                                                        >  1:667821/1‑149 (MQ=255)
          tgtAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGttttt                                                                                                                                        <  1:174156/149‑1 (MQ=255)
          tgtAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACCGCGAAGTGGGtttca                                                                                                                                        <  3:1058556/149‑3 (MQ=255)
              gttgttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTttgtt                                                                                                                                    <  1:1235262/149‑1 (MQ=255)
              gttgttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTttgtt                                                                                                                                    <  1:310365/149‑1 (MQ=255)
                tgttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTtgttgt                                                                                                                                  <  3:593804/149‑1 (MQ=255)
                 gttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTc                                                                                                                                 <  4:100992/149‑1 (MQ=255)
                  ttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCa                                                                                                                                <  3:1319276/149‑1 (MQ=255)
                  ttAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCa                                                                                                                                <  4:706477/149‑1 (MQ=255)
                         cGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGttt                                                                                                                         <  3:114308/149‑1 (MQ=255)
                         cGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACCATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGttt                                                                                                                         <  4:1111574/149‑1 (MQ=255)
                          gTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGtttttt                                                                                                                                       <  2:357268/134‑1 (MQ=255)
                          gTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGtttttt                                                                                                                                       >  1:357268/1‑134 (MQ=255)
                            acacTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTtaa                                                                                                                      <  4:159011/149‑1 (MQ=255)
                             cacTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTtaat                                                                                                                     >  2:542789/1‑149 (MQ=255)
                             cacTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTtaat                                                                                                                     <  1:1379519/149‑1 (MQ=255)
                              acTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTtaata                                                                                                                    <  1:40792/149‑1 (MQ=255)
                                tctGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATaaa                                                                                                                  <  3:1655584/149‑1 (MQ=255)
                                tctGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATaaa                                                                                                                  <  1:432137/149‑1 (MQ=255)
                                 ctGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAAt                                                                                                                 <  2:192111/149‑1 (MQ=255)
                                       cAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCaga                                                                                                           >  4:625944/1‑149 (MQ=255)
                                       cAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCaga                                                                                                           <  2:12617/149‑1 (MQ=255)
                                        agagATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTc                                                                                                                                 >  2:244556/1‑126 (MQ=255)
                                        agagATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCagag                                                                                                          >  1:1598075/1‑149 (MQ=255)
                                         gagaTGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGc                                                                                                         >  3:514550/1‑149 (MQ=255)
                                          agaTGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCa                                                                                                        >  1:476455/1‑149 (MQ=255)
                                             tgtgATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAAc                                                                                                     <  4:745310/149‑1 (MQ=255)
                                                gATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTc                                                                                                  >  3:539099/1‑149 (MQ=255)
                                                  tGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTttgtt                                                                                                                                    <  4:1130939/113‑1 (MQ=255)
                                                   ggTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTTTGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCATGAGTTTTAATAAATGGCAGAGCAAACCTcgcg                                                                                               >  4:88205/1‑149 (MQ=255)
                                                     tgtCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGtc                                                                                             >  4:539397/1‑149 (MQ=255)
                                                       tCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGtctc                                                                                           >  2:737530/1‑149 (MQ=255)
                                                       tCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGtctc                                                                                           >  1:1437377/1‑149 (MQ=255)
                                                       tCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGtctc                                                                                           >  1:838305/1‑149 (MQ=255)
                                                         tttAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCa                                                                                                                                >  3:368770/1‑110 (MQ=255)
                                                         tttAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAAt                                                                                                                 <  3:402658/125‑1 (MQ=255)
                                                            aattaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCaat                                                                                      >  1:1635037/1‑149 (MQ=255)
                                                             attaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTAAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCaata                                                                                     <  2:166702/149‑1 (MQ=255)
                                                              ttaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTTGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCaataa                                                                                    >  3:634113/1‑149 (MQ=255)
                                                              ttaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCaga                                                                                                           >  3:1281140/1‑126 (MQ=255)
                                                              ttaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCaga                                                                                                           <  4:1281140/126‑1 (MQ=255)
                                                              ttaaATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCaataa                                                                                    >  4:1477936/1‑149 (MQ=255)
                                                                aaaTCGATGATGCTCCTGGAGACAAGCATTGCGTGGACTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAatt                                                                                  <  3:958014/149‑1 (MQ=255)
                                                                 aaTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTGCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAatta                                                                                 <  2:1322142/149‑1 (MQ=255)
                                                                  aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTggg                                                                                                                                             >  1:873769/1‑88 (MQ=255)
                                                                  aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTggg                                                                                                                                             <  2:873769/88‑1 (MQ=255)
                                                                  aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAattat                                                                                <  2:1437377/149‑1 (MQ=255)
                                                                  aTCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAattat                                                                                <  1:223372/149‑1 (MQ=255)
                                                                   tCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAattatt                                                                               <  3:1273069/149‑1 (MQ=255)
                                                                     gatgatGCTCCTGGAGAAAAGCATTGCGTGGCCTTGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGa                                                                             <  4:634113/149‑1 (MQ=255)
                                                                     gatgatGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGa                                                                             >  4:1227606/1‑149 (MQ=255)
                                                                           gCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCAc                                                                       >  3:1082272/1‑149 (MQ=255)
                                                                             tCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTg                                                                     >  3:257905/1‑149 (MQ=255)
                                                                             tCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTg                                                                     >  2:1015001/1‑149 (MQ=255)
                                                                              ccTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGGACTgg                                                                    <  4:355156/149‑1 (MQ=255)
                                                                               cTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGa                                                                   >  2:796083/1‑149 (MQ=255)
                                                                                 ggAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGc                                                                 <  3:555860/149‑1 (MQ=255)
                                                                                  gagaAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCg                                                                >  1:1350582/1‑149 (MQ=255)
                                                                                     aaaaGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGaaa                                                             >  3:1587177/1‑149 (MQ=255)
                                                                                     aaaaGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGaaa                                                             >  4:403649/1‑149 (MQ=255)
                                                                                       aaGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGaaaaa                                                           >  3:1376871/1‑149 (MQ=255)
                                                                                       aaGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGaaaaa                                                           >  2:1566648/1‑149 (MQ=255)
                                                                                           aTTGCGTGGCCTCGTATTCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCTCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAGCCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAaataat                                                       <  4:1374713/149‑1 (MQ=255)
                                                                                            ttGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATg                                                      <  3:625944/149‑1 (MQ=255)
                                                                                                     cTCGTAATCGCTATATCTACTATTATGTCGCCTGGAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTTACAATc                                             >  3:1124153/1‑149 (MQ=255)
                                                                                                     cTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAaataa                                                        >  2:680543/1‑138 (MQ=255)
                                                                                                     cTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATc                                             >  4:1510942/1‑149 (MQ=255)
                                                                                                      tCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCt                                            <  3:1510942/149‑1 (MQ=255)
                                                                                                        gTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGa                                          >  4:1329273/1‑149 (MQ=255)
                                                                                                         tAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAc                                         <  4:1241350/149‑1 (MQ=255)
                                                                                                         tAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAc                                         <  4:1313199/149‑1 (MQ=255)
                                                                                                         tAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGAc                                         <  3:175986/149‑1 (MQ=255)
                                                                                                               cTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACctcctc                                   >  4:291092/1‑149 (MQ=255)
                                                                                                                  tatCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCAtt                                >  1:1043161/1‑149 (MQ=255)
                                                                                                                   atCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTa                               <  2:1108059/149‑1 (MQ=255)
                                                                                                                      tactaTTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCt                                            >  3:74430/1‑133 (MQ=255)
                                                                                                                      tactaTTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTc                            >  1:1533781/1‑149 (MQ=255)
                                                                                                                           ttatGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGa                                                                             >  1:699647/1‑95 (MQ=255)
                                                                                                                             atGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGa                     <  2:119883/149‑1 (MQ=255)
                                                                                                                              tGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGAc                    <  2:1634667/149‑1 (MQ=255)
                                                                                                                                tCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAaataat                                                       >  4:496754/1‑112 (MQ=255)
                                                                                                                                  gCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAg                >  3:901626/1‑149 (MQ=255)
                                                                                                                                    cTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTg                                                                     >  4:530317/1‑94 (MQ=255)
                                                                                                                                     tGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCt             >  3:1583691/1‑149 (MQ=255)
                                                                                                                                     tGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCt             >  4:1395891/1‑149 (MQ=255)
                                                                                                                                       aaaCCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTgg           <  3:88205/149‑1 (MQ=255)
                                                                                                                                       aaaCCCACCGCGAAGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGa                                                                   <  1:637643/93‑1 (MQ=255)
                                                                                                                                       aaaCCCACCGCGAAGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGa                     <  3:822724/139‑1 (MQ=255)
                                                                                                                                       aaaCCCACCGCGAAGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGa                     >  4:822724/1‑139 (MQ=255)
                                                                                                                                       aaaCCCACCGCGAAGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTgg           >  4:1676074/1‑149 (MQ=255)
                                                                                                                                           ccACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTa                               >  4:384008/1‑125 (MQ=255)
                                                                                                                                             aCTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAAc     >  1:1160926/1‑149 (MQ=255)
                                                                                                                                               ttCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAaata                                                         >  4:444104/1‑95 (MQ=255)
                                                                                                                                               ttCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAaata                                                         <  3:444104/95‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GAGCAGTGTGTGTAGTTGTTAATTACGTACACTCTGTTACAGAGATGTGATGGTGTCTTTAATTAAATCGATGATGCTCCTGGAGAAAAGCATTGCGTGGCCTCGTAATCGCTATATCTACTATTATGTCGCCTGAAACCCACTTCGCGGTGGGTTTTTTGTTGTCAGGAGTTTTAATAAATGGCAGAGCAAACCTCGCGTCTCGCAATAATTATTGATAGCACTGGAGCGAAAAATAATGCTGACAATCTGACCTCCTCATTAGTCAAAATGACGCAGGCTGGGGAAACTGC  >  NC_000913/1426274‑1426566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: