Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,829,334 G→T 5.9% P134Q (CCG→CAG)  astD ← succinylglutamic semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,829,3340GT 5.9% 600.8 / 20.3 169P134Q (CCG→CAG) astDsuccinylglutamic semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (81/78);  new base T (7/3);  total (88/81)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.02e-01

Only 100 of 169 total aligned reads displayed.

ACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTC  >  NC_000913/1829189‑1829481
                                                                                                                                                 |                                                                                                                                                   
  gCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                                <  2:418722/149‑1 (MQ=255)
  gCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                                <  3:1068628/149‑1 (MQ=255)
  gCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                                >  3:1502523/1‑149 (MQ=255)
  gCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGa                                                                                                                                                >  3:1543844/1‑149 (MQ=255)
   cATTACCGCTTCGCCACTCCACGGTGTCAGTTCTCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                               <  4:1114938/149‑3 (MQ=255)
   cATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGAt                                                                                                                                               <  4:637019/149‑1 (MQ=255)
    aTTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATg                                                                                                                                              >  4:212148/1‑149 (MQ=255)
     tgcccGCTTCGCCAGTCCACGGTGTCAGTTCGATGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGTAACGATATGTCCGTTCGGCAAAGGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGt                                                                                                                                             <  3:237400/146‑1 (MQ=255)
              cGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGc                                                                                                                                          >  4:129479/1‑143 (MQ=255)
               gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                               <  2:712179/137‑3 (MQ=255)
               gCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                               >  1:712179/1‑135 (MQ=255)
                ccACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgc                                                                                                                                  >  1:761412/1‑149 (MQ=255)
                      cACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgcc                                                                                                                            >  2:1214241/1‑149 (MQ=255)
                      cACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTcgccgcgcc                                                                                                                            >  3:504621/1‑149 (MQ=255)
                        cGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGt                                                                                                                          <  3:1392090/149‑1 (MQ=255)
                          gtgtCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTcc                                                                                                                        <  2:761412/149‑1 (MQ=255)
                            gtCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCgg                                                                                                                      <  2:219560/149‑1 (MQ=255)
                             tCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGc                                                                                                                     <  3:1254841/149‑1 (MQ=255)
                               aGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGACGTCGCAGGCTCGCCGCGCCGTCCGGCAt                                                                                                                   <  4:1509490/149‑1 (MQ=255)
                                     cTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCCGCACGGCGTGCTGGCggt                                                                                                                                               <  1:218064/115‑3 (MQ=255)
                                          tttAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                        >  1:977497/1‑149 (MQ=255)
                                          tttAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                        >  3:1640095/1‑149 (MQ=255)
                                              aaGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTc                                                                                                    >  3:231216/1‑149 (MQ=255)
                                               aGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAttt                                                                                                                 <  2:183363/135‑1 (MQ=255)
                                               aGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCAttt                                                                                                                 >  1:183363/1‑135 (MQ=255)
                                               aGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCg                                                                                                   >  2:1138100/1‑149 (MQ=255)
                                                gATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGc                                                                                                  >  1:1187612/1‑149 (MQ=255)
                                                gATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGc                                                                                                  >  1:352057/1‑149 (MQ=255)
                                                gATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGc                                                                                                  >  2:1644632/1‑149 (MQ=255)
                                                 aTAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGcc                                                                                                 <  2:352057/149‑1 (MQ=255)
                                                 aTAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGcc                                                                                                 <  4:311507/149‑1 (MQ=255)
                                                       gtgtTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACg                                                                                           >  3:1625027/1‑149 (MQ=255)
                                                       gtgtTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACg                                                                                           >  4:570600/1‑149 (MQ=255)
                                                          ttACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                        <  2:1583512/133‑1 (MQ=255)
                                                          ttACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACgct                                                                                                        >  1:1583512/1‑133 (MQ=255)
                                                          ttACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAAc                                                                                        <  3:136782/149‑1 (MQ=255)
                                                             ccTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTg                                                                                     <  1:1019467/149‑1 (MQ=255)
                                                              cTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGa                                                                                    >  3:685976/1‑149 (MQ=255)
                                                              cTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGa                                                                                    >  3:98973/1‑149 (MQ=255)
                                                              cTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGa                                                                                    >  1:112073/1‑149 (MQ=255)
                                                               tGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGAt                                                                                   >  4:1432/1‑149 (MQ=255)
                                                                 ccAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATAc                                                                                 <  3:453694/149‑1 (MQ=255)
                                                                 ccAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATAc                                                                                 >  2:1037218/1‑149 (MQ=255)
                                                                         gCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAAt                                                                         <  2:1430342/149‑1 (MQ=255)
                                                                          ccGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAAtt                                                                        <  3:369850/149‑1 (MQ=255)
                                                                           cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                       >  1:1233079/1‑149 (MQ=255)
                                                                           cGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTg                                                                       <  4:1034175/149‑1 (MQ=255)
                                                                            ggAACGATATGTCCGTGCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCGGGCTCGCAGCGCCGTCCCGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGa                                                                      <  3:297253/149‑1 (MQ=255)
                                                                              aaCGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGata                                                                    <  1:134486/149‑1 (MQ=255)
                                                                                 gATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                                  >  4:181119/1‑132 (MQ=255)
                                                                                 gATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATa                                                                                  <  3:181119/132‑1 (MQ=255)
                                                                                   tatGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcgcg                                                               >  2:631235/1‑149 (MQ=255)
                                                                                          gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcg                                                                 >  3:420154/1‑140 (MQ=255)
                                                                                          gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATcg                                                                 <  4:420154/140‑1 (MQ=255)
                                                                                          gTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAt                                                        <  3:1679070/149‑1 (MQ=255)
                                                                                           ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       <  1:924484/149‑1 (MQ=255)
                                                                                           ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       <  1:26340/149‑1 (MQ=255)
                                                                                           ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       <  4:1625027/149‑1 (MQ=255)
                                                                                           ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       <  4:1543844/149‑1 (MQ=255)
                                                                                           ttCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       >  2:1687188/1‑149 (MQ=255)
                                                                                              ggCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGat                                                    >  3:786661/1‑149 (MQ=255)
                                                                                              ggCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGat                                                    <  4:1640095/149‑1 (MQ=255)
                                                                                              ggCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGat                                                    <  1:1068609/149‑1 (MQ=255)
                                                                                                 aaaTGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGa                                                              <  4:1378570/136‑1 (MQ=255)
                                                                                                  aaTGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGatcatc                                                <  4:891907/149‑1 (MQ=255)
                                                                                                  aaTGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGat                                                    <  3:156041/145‑1 (MQ=255)
                                                                                                      aCCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCg                                            <  3:10487/149‑1 (MQ=255)
                                                                                                         aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCAc                                                                                                              <  1:542882/80‑1 (MQ=255)
                                                                                                         aGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCa                                         >  4:1207844/1‑149 (MQ=255)
                                                                                                           ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCa                                                                                                                                         <  2:1407961/51‑1 (MQ=255)
                                                                                                           ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGat                                                                     <  2:30263/119‑1 (MQ=255)
                                                                                                           ggAAATTATACGGCCCAAACACCGCCAGCACGCCGTGAGGGCGATGTCGCa                                                                                                                                         >  1:1407961/1‑51 (MQ=255)
                                                                                                             aaaTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCACCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACtgc                                     >  2:496646/1‑147 (MQ=255)
                                                                                                               aTTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGTTACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCgg                                   >  3:884065/1‑149 (MQ=255)
                                                                                                                ttATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGt                                  <  3:1201526/149‑1 (MQ=255)
                                                                                                                ttATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGt                                  >  1:852419/1‑149 (MQ=255)
                                                                                                                   taCGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGc                               <  3:600201/149‑1 (MQ=255)
                                                                                                                      ggCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGc                            >  3:1032238/1‑149 (MQ=255)
                                                                                                                       gCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCt                           >  4:1668727/1‑149 (MQ=255)
                                                                                                                        cccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCtt                          >  2:1553583/1‑149 (MQ=255)
                                                                                                                        cccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCtt                          <  2:112073/149‑1 (MQ=255)
                                                                                                                         ccAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTc                         <  3:1432/149‑1 (MQ=255)
                                                                                                                          cAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTcc                        >  3:742193/1‑149 (MQ=255)
                                                                                                                            aaCACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCa                      >  2:938335/1‑149 (MQ=255)
                                                                                                                            aaCACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCa                      >  2:467700/1‑149 (MQ=255)
                                                                                                                            aaCACCGCCAGCACGCCGTACGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       >  3:993512/1‑116 (MQ=255)
                                                                                                                             acatCGCCCGCACGGCGTGCTGGCGGTGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAg                     >  1:932395/1‑149 (MQ=255)
                                                                                                                               atcGCCCGCACGGCGTGCTGGCGGTGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAgcg                   >  4:149093/3‑149 (MQ=255)
                                                                                                                                  gCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCgg                >  4:277888/1‑149 (MQ=255)
                                                                                                                                    cAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCt              <  3:1668727/149‑1 (MQ=255)
                                                                                                                                      gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATc                                                                  >  1:1148326/1‑95 (MQ=255)
                                                                                                                                      gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc               <  4:1285537/146‑1 (MQ=255)
                                                                                                                                      gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGc               >  3:1285537/1‑146 (MQ=255)
                                                                                                                                      gCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTa            >  4:301675/1‑149 (MQ=255)
                                                                                                                                        aCGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTAcc          <  4:508176/149‑1 (MQ=255)
                                                                                                                                         cGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTAccc         <  3:827299/149‑1 (MQ=255)
                                                                                                                                          gCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGt                                                                                      >  1:1674291/1‑71 (MQ=255)
                                                                                                                                          gCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTAtt                                                       >  3:234314/1‑102 (MQ=255)
                                                                                                                                                cGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTtctc  <  2:211586/149‑1 (MQ=255)
                                                                                                                                                cGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTtctc  <  1:1037218/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ACGCATTACCGCTTCGCCACTCCACGGTGTCAGTTCGCTGGGTTTAAAGATAATGGTGTTACCTGCCAGCAATGCCGGAACGATATGTCCGTTCGGCAAATGACCAGGGAAATTATACGGCCCAAACACCGCCAGCACGCCGTGCGGGCGATGTCGCAGGCTCGCCGCGCCGTCCGGCATTTCACTACGCTGCTCGCCGGTACGAACGTGATACGCCTTAATTGATATCGCGATTTTATTGATCATCGCCGTCACTTCGGTTGCCGCTTCCCAGCGCGGCTTACCCGTTTCTC  >  NC_000913/1829189‑1829481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: