Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,891,841 A→G 6.6% G465G (GGT→GGC) ‡ yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,891,8410AG 6.6% 622.4 / 24.5 196G465G (GGT→GGC) ‡yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base A (94/89);  new base G (6/7);  total (100/96)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.60e-01

Only 100 of 202 total aligned reads displayed.

GAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCT  >  NC_000913/1891693‑1891989
                                                                                                                                                    |                                                                                                                                                    
gaagCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTGCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGa                                                                                                                                                      >  1:1104711/1‑149 (MQ=255)
gaagCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGa                                                                                                                                                      <  1:647436/149‑1 (MQ=255)
    cgcATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgt                                                                                                                                                  >  2:1677746/1‑149 (MQ=255)
     gcATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgtt                                                                                                                                                 >  1:572633/1‑149 (MQ=255)
      cATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttg                                                                                                                                                >  1:1223540/1‑149 (MQ=255)
        ttACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttgtt                                                                                                                                              <  3:433304/149‑1 (MQ=255)
         tACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgctt                                                                                                                                             <  2:1476145/149‑4 (MQ=255)
            cGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgttttat                                                                                                                                          <  3:948026/149‑5 (MQ=255)
            cGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCt                                                                                                                                          <  4:1077310/149‑1 (MQ=255)
                gcTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCga                                                                                                                                      <  1:1270458/149‑1 (MQ=255)
                  tGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatg                                                                                                                                    >  4:773140/1‑149 (MQ=255)
                      aaaTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCg                                                                                                                                >  2:70268/1‑149 (MQ=255)
                       aaTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCgg                                                                                                                               <  2:1278062/149‑1 (MQ=255)
                        aTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGt                                                                                                                              <  1:1577520/149‑1 (MQ=255)
                         ttGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTc                                                                                                                             <  1:976071/149‑1 (MQ=255)
                             tgcAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAACGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTc                                                                                                                             >  3:1401069/1‑145 (MQ=255)
                             tgcAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAACGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTc                                                                                                                             <  4:1401069/145‑1 (MQ=255)
                                  cAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatgat                                                                                                                                  >  4:1610239/1‑135 (MQ=255)
                                        cccTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGAGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatgat                                                                                                                                  >  4:1343244/1‑129 (MQ=255)
                                                tGGTTTCCCCCTGCAACAATACAGGAAGGGTCATGGTAGAGCGGAACTGCTCGGCCAGATCGCGCACCATGGCGTGCTAGGTACAGAGAATTAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      >  2:1228110/1‑149 (MQ=255)
                                                tGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      >  3:1293117/1‑149 (MQ=255)
                                                  gTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGc                                                                                                    >  4:862336/1‑149 (MQ=255)
                                                     tCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGa                                                                                                 <  4:475307/149‑1 (MQ=255)
                                                       ccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgtttta                                                                                                                                           <  3:1613638/105‑4 (MQ=255)
                                                       ccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttgtt                                                                                                                                              <  3:1348981/102‑1 (MQ=255)
                                                        cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGTGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              >  3:1648195/1‑149 (MQ=255)
                                                        cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              <  2:55005/149‑1 (MQ=255)
                                                         ccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATc                                                                                                                                 >  3:380956/1‑113 (MQ=255)
                                                         ccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATc                                                                                                                                 <  4:380956/113‑1 (MQ=255)
                                                         ccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACct                                                                                             <  3:653525/149‑1 (MQ=255)
                                                          cTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACctg                                                                                            >  3:1483845/1‑149 (MQ=255)
                                                            gCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACctggc                                                                                          >  4:691312/1‑149 (MQ=255)
                                                            gCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACctggc                                                                                          >  4:1668541/1‑149 (MQ=255)
                                                                caaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggt                                                                                      <  3:803746/149‑1 (MQ=255)
                                                                 aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAGCGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  2:441764/149‑1 (MQ=255)
                                                                 aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  1:1631238/149‑1 (MQ=255)
                                                                 aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  2:997954/149‑1 (MQ=255)
                                                                  aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    <  2:816283/149‑1 (MQ=255)
                                                                  aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    >  4:1201654/1‑149 (MQ=255)
                                                                  aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    <  2:1077329/149‑1 (MQ=255)
                                                                   tACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgtttta                                                                                                                                           <  4:952173/93‑4 (MQ=255)
                                                                    aCAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttggt                                                                                  <  3:117030/149‑1 (MQ=255)
                                                                        gAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGc                                                                              >  4:1549651/1‑149 (MQ=255)
                                                                            cGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCa                                                                          <  1:425864/149‑1 (MQ=255)
                                                                             gTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGTTCGCAGCAGTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAg                                                                         >  3:1455046/1‑149 (MQ=255)
                                                                             gTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     >  2:489087/1‑137 (MQ=255)
                                                                             gTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCAGTGCCGCTAACTGGCGAGCAGAACCTggttggtt                                                                                     <  1:489087/137‑1 (MQ=255)
                                                                              tCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGa                                                                        <  1:70268/149‑1 (MQ=255)
                                                                                aTGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGAtt                                                                      >  1:1323935/1‑149 (MQ=255)
                                                                                        gcgGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCgcagca                                                              >  3:728916/1‑149 (MQ=255)
                                                                                            aaCTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGTAGCAGATTGCGCAGcacaca                                                          <  2:295767/149‑1 (MQ=255)
                                                                                            aaCTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGcacaca                                                          >  4:1512582/1‑149 (MQ=255)
                                                                                            aaCTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGcacaca                                                          >  2:1432684/1‑149 (MQ=255)
                                                                                              cTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACaga                                                        <  1:1413578/149‑1 (MQ=255)
                                                                                              cTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACaga                                                        <  3:1668541/149‑1 (MQ=255)
                                                                                              cTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACaga                                                        <  1:88215/149‑1 (MQ=255)
                                                                                                gCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGt                                                      >  4:1323660/1‑149 (MQ=255)
                                                                                                gCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGt                                                      >  2:1147181/1‑149 (MQ=255)
                                                                                                  tCGGCCAGATCGCGCATCATGACGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTaa                                                    <  4:1221933/149‑1 (MQ=255)
                                                                                                        aGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTg                                              <  1:1688420/149‑1 (MQ=255)
                                                                                                           tCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcg                                           <  3:1343630/149‑1 (MQ=255)
                                                                                                              cgcATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCt                                        <  1:811945/149‑1 (MQ=255)
                                                                                                              cgcATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCt                                        <  4:726499/149‑1 (MQ=255)
                                                                                                               gcATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTg                                       <  4:531309/149‑1 (MQ=255)
                                                                                                                catcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGt                                      <  4:1293117/149‑1 (MQ=255)
                                                                                                                catcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGt                                      >  1:335721/1‑149 (MQ=255)
                                                                                                                 atcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTa                                     <  4:422453/149‑1 (MQ=255)
                                                                                                                  tcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTaa                                    <  1:118850/149‑1 (MQ=255)
                                                                                                                       gCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCaaa                               <  3:27685/149‑1 (MQ=255)
                                                                                                                        cGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAt                              <  4:264325/149‑1 (MQ=255)
                                                                                                                             gAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAtgggct                         <  3:208245/149‑1 (MQ=255)
                                                                                                                              aGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcggc                                         <  4:1350837/132‑1 (MQ=255)
                                                                                                                                 tACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      >  3:1609577/1‑68 (MQ=255)
                                                                                                                                 tACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAtgggctgggc                     <  4:574982/149‑1 (MQ=255)
                                                                                                                                  aCAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCa                    >  3:1091927/1‑149 (MQ=255)
                                                                                                                                   cAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCaa                   >  3:158911/1‑149 (MQ=255)
                                                                                                                                   cAAAGCATAAAACAACGACCGTTGTTAGCTTAGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAAAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCaa                   >  2:948935/1‑149 (MQ=255)
                                                                                                                                    aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTa                                     >  1:1027952/1‑130 (MQ=255)
                                                                                                                                    aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTa                                     <  2:1027952/130‑1 (MQ=255)
                                                                                                                                    aaaGCAGAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAAc                  >  2:1218472/1‑149 (MQ=255)
                                                                                                                                     aaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACa                 >  3:564083/1‑149 (MQ=255)
                                                                                                                                     aaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACa                 <  2:1641613/149‑1 (MQ=255)
                                                                                                                                        cATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCGGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCa              >  2:665034/1‑149 (MQ=255)
                                                                                                                                        aagctaacaacGGTCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcggc                                         <  4:1535252/117‑1 (MQ=255)
                                                                                                                                         aTAAAACACCGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCTGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACAGAAAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             <  4:1038353/149‑1 (MQ=255)
                                                                                                                                         aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGa                                                                        <  2:1198752/90‑1 (MQ=255)
                                                                                                                                         aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             >  3:105676/1‑149 (MQ=255)
                                                                                                                                         aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             <  2:646562/149‑1 (MQ=255)
                                                                                                                                         aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             >  3:1283547/1‑149 (MQ=255)
                                                                                                                                          tAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAtgggct                         >  3:513193/1‑136 (MQ=255)
                                                                                                                                          tAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCCGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAAc            >  4:1124152/1‑149 (MQ=255)
                                                                                                                                          gctaacaacGGTCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAAc            >  3:1235600/4‑149 (MQ=255)
                                                                                                                                           aaaaCAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACg           >  4:217924/1‑149 (MQ=255)
                                                                                                                                            aaaCAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGa          >  2:345024/1‑149 (MQ=255)
                                                                                                                                               caacGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTc       <  4:564083/149‑1 (MQ=255)
                                                                                                                                                aacGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCg      >  1:1088820/1‑149 (MQ=255)
                                                                                                                                                   gACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGcc   <  4:616071/149‑1 (MQ=255)
                                                                                                                                                   gACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGcc   >  1:167232/1‑149 (MQ=255)
                                                                                                                                                    aCCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCt  <  3:1310991/149‑1 (MQ=255)
                                                                                                                                                    aCCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCt  <  1:1143689/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCT  >  NC_000913/1891693‑1891989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: