Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,891,842 C→T 6.6% G465D (GGT→GAT) ‡ yoaA ← putative ATP‑dependent helicase, DinG family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,891,8420CT 6.6% 637.0 / 22.0 197G465D (GGT→GAT) ‡yoaAputative ATP‑dependent helicase, DinG family
Reads supporting (aligned to +/- strand):  ref base C (94/90);  new base T (6/7);  total (100/97)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 198 total aligned reads displayed.

AAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCT  >  NC_000913/1891694‑1891989
                                                                                                                                                    |                                                                                                                                                   
   cgcATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgt                                                                                                                                                  >  2:1677746/1‑149 (MQ=255)
    gcATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgtt                                                                                                                                                 >  1:1031621/1‑149 (MQ=255)
    gcATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgtt                                                                                                                                                 >  1:572633/1‑149 (MQ=255)
     cATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttg                                                                                                                                                >  1:1223540/1‑149 (MQ=255)
      aTTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttgt                                                                                                                                               >  4:358590/1‑149 (MQ=255)
       ttACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttgtt                                                                                                                                              <  4:1358116/149‑1 (MQ=255)
        tACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgctt                                                                                                                                             <  2:1476145/149‑4 (MQ=255)
           cGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgttttat                                                                                                                                          <  1:109322/149‑5 (MQ=255)
               gcTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCga                                                                                                                                      <  1:1270458/149‑1 (MQ=255)
                 tGACAAATTGGTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGATAGCGCGGAACTGCTCGGCAAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatg                                                                                                                                    >  3:1038353/1‑149 (MQ=255)
                 tGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatg                                                                                                                                    >  3:240427/1‑149 (MQ=255)
                    cAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATc                                                                                                                                 <  3:879006/149‑1 (MQ=255)
                     aaaTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCg                                                                                                                                >  2:70268/1‑149 (MQ=255)
                     aaaTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCg                                                                                                                                >  3:1244530/1‑149 (MQ=255)
                      aaTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCgg                                                                                                                               <  2:1278062/149‑1 (MQ=255)
                       aTTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGt                                                                                                                              <  1:1577520/149‑1 (MQ=255)
                        ttGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTc                                                                                                                             <  1:976071/149‑1 (MQ=255)
                             gcAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCgcagc                                                                                                                        >  3:574982/1‑149 (MQ=255)
                               aaCAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCAt                                                                                                                      >  3:177913/1‑149 (MQ=255)
                                 cAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCgatgat                                                                                                                                  <  3:1610239/135‑1 (MQ=255)
                                  aGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGc                                                                                                                   <  4:112369/149‑1 (MQ=255)
                                  aGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGATAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGc                                                                                                                   <  4:151570/149‑1 (MQ=255)
                                    ttGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCg                                                                                                                 >  4:803746/1‑149 (MQ=255)
                                    ttGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCg                                                                                                                 >  4:410849/1‑149 (MQ=255)
                                              cTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCg                                                                                                       >  4:653525/1‑149 (MQ=255)
                                               tGGTTTCCCCCTGCAACAATACAGGAAGGGTCATGGTAGAGCGGAACTGCTCGGCCAGATCGCGCACCATGGCGTGCTAGGTACAGAGAATTAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      >  2:1228110/1‑149 (MQ=255)
                                                  tttCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTTTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCa                                                                                                   >  4:1541218/1‑149 (MQ=255)
                                                     cccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGaa                                                                                                >  3:1448614/1‑149 (MQ=255)
                                                     cccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGaa                                                                                                <  3:1198685/149‑1 (MQ=255)
                                                      ccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgtttta                                                                                                                                           >  4:1613638/1‑102 (MQ=255)
                                                      ccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCgttgtt                                                                                                                                              >  4:1348981/1‑102 (MQ=255)
                                                      ccccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAc                                                                                               >  4:117030/1‑149 (MQ=255)
                                                       cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              >  3:1622718/1‑149 (MQ=255)
                                                       cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              >  4:537557/1‑149 (MQ=255)
                                                       cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              >  4:579019/1‑149 (MQ=255)
                                                       cccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAAcc                                                                                              <  2:55005/149‑1 (MQ=255)
                                                        ccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACct                                                                                             >  3:422453/1‑149 (MQ=255)
                                                        ccTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACct                                                                                             >  3:145697/1‑149 (MQ=255)
                                                                aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAGCGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  2:441764/149‑1 (MQ=255)
                                                                aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  4:968040/149‑1 (MQ=255)
                                                                aaTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     <  1:1631238/149‑1 (MQ=255)
                                                                 aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    <  2:816283/149‑1 (MQ=255)
                                                                 aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    >  3:1348479/1‑149 (MQ=255)
                                                                 aTACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttg                                                                                    <  2:1077329/149‑1 (MQ=255)
                                                                  tACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGGTCgttgtttta                                                                                                                                           >  3:952173/1‑90 (MQ=255)
                                                                  tACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggttgg                                                                                   <  3:1299083/149‑1 (MQ=255)
                                                                    cAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTggttggtt                                                                                 <  4:890522/149‑1 (MQ=255)
                                                                    cAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTggttggtt                                                                                 <  4:1221344/149‑1 (MQ=255)
                                                                         aGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCgg                                                                            <  3:160203/149‑1 (MQ=255)
                                                                           cGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCa                                                                          <  1:425864/149‑1 (MQ=255)
                                                                            gTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCtggtt                                                                                     >  2:489087/1‑137 (MQ=255)
                                                                            gTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCAGTGCCGCTAACTGGCGAGCAGAACCTggttggtt                                                                                     <  1:489087/137‑1 (MQ=255)
                                                                             tCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGa                                                                        <  2:980952/149‑1 (MQ=255)
                                                                             tCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGa                                                                        <  1:70268/149‑1 (MQ=255)
                                                                               aTGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGAtt                                                                      >  1:1323935/1‑149 (MQ=255)
                                                                                           aaCTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGTAGCAGATTGCGCAGcacaca                                                          <  2:295767/149‑1 (MQ=255)
                                                                                           aaCTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGcacaca                                                          >  2:1432684/1‑149 (MQ=255)
                                                                                             cTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACaga                                                        <  1:88215/149‑1 (MQ=255)
                                                                                             cTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACaga                                                        <  1:1413578/149‑1 (MQ=255)
                                                                                              tGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACagag                                                       >  3:80188/1‑149 (MQ=255)
                                                                                               gCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGt                                                      >  2:1147181/1‑149 (MQ=255)
                                                                                                 tCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCg                                                                                                       <  3:470054/98‑1 (MQ=255)
                                                                                                 tCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCg                                                                                                       >  4:470054/1‑98 (MQ=255)
                                                                                                 tCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTaa                                                    <  3:358590/149‑1 (MQ=255)
                                                                                                 tCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTaa                                                    >  3:531309/1‑149 (MQ=255)
                                                                                                   ggCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      <  3:1288578/97‑1 (MQ=255)
                                                                                                   ggCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      >  4:1288578/1‑97 (MQ=255)
                                                                                                      cAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCt                                               >  3:685334/1‑149 (MQ=255)
                                                                                                      cAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCt                                               >  4:184101/1‑149 (MQ=255)
                                                                                                       aGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTg                                              <  1:1688420/149‑1 (MQ=255)
                                                                                                        gATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTgg                                             >  3:164961/1‑149 (MQ=255)
                                                                                                          tCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcg                                           <  3:1343630/149‑1 (MQ=255)
                                                                                                             cgcATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCt                                        <  1:811945/149‑1 (MQ=255)
                                                                                                             cgcATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCt                                        <  4:240427/149‑1 (MQ=255)
                                                                                                               catcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGt                                      >  1:335721/1‑149 (MQ=255)
                                                                                                                 tcatGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTaa                                    <  1:118850/149‑1 (MQ=255)
                                                                                                                     ggCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCaa                                <  4:1338359/149‑1 (MQ=255)
                                                                                                                       cGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAt                              <  4:723407/149‑1 (MQ=255)
                                                                                                                             aGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcggc                                         >  3:1350837/1‑132 (MQ=255)
                                                                                                                                tACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGa                                                                                                      <  4:1609577/68‑1 (MQ=255)
                                                                                                                                   aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCa                                                                          <  3:1075049/93‑1 (MQ=255)
                                                                                                                                   aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCa                                                                          >  4:1075049/1‑93 (MQ=255)
                                                                                                                                   aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTa                                     >  1:1027952/1‑130 (MQ=255)
                                                                                                                                   aaaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTa                                     <  2:1027952/130‑1 (MQ=255)
                                                                                                                                   aaaGCAGAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAAc                  >  2:1218472/1‑149 (MQ=255)
                                                                                                                                    aaGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACa                 <  2:1641613/149‑1 (MQ=255)
                                                                                                                                       cATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCGGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCa              >  2:665034/1‑149 (MQ=255)
                                                                                                                                       aagctaacaacGGTCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTggcggc                                         >  3:1535252/6‑122 (MQ=255)
                                                                                                                                        aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGa                                                                        <  2:1198752/90‑1 (MQ=255)
                                                                                                                                        aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             >  4:568661/1‑149 (MQ=255)
                                                                                                                                        aTAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCaa             <  2:646562/149‑1 (MQ=255)
                                                                                                                                         tAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAAtgggct                         <  4:513193/136‑1 (MQ=255)
                                                                                                                                          aaaaCAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGa          <  4:752213/149‑1 (MQ=255)
                                                                                                                                           aaaCAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGa          >  2:345024/1‑149 (MQ=255)
                                                                                                                                             acaacGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACt        >  4:1310991/1‑149 (MQ=255)
                                                                                                                                               aacGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCg      >  3:1134496/1‑149 (MQ=255)
                                                                                                                                               aacGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCg      <  4:1448614/149‑1 (MQ=255)
                                                                                                                                                  gACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGcc   >  1:167232/1‑149 (MQ=255)
                                                                                                                                                   aCCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCt  <  3:1310991/149‑1 (MQ=255)
                                                                                                                                                   aCCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCt  <  1:1143689/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGCGCATTACCGGCGCTGACAAATTGCTGCAACAGTTGCCCTTTGCTGGTTTCCCCCTGCAACAATACAGGAAGCGTCATGGTAGCGCGGAACTGCTCGGCCAGATCGCGCATCATGGCGTGCGAGGTACAAAGCATAAAACAACGACCGTTGTTAGCTTCGATGATCGGTCGCAGCATTGCCGCTAACTGGCGAGCAGAACCTGGCTGGTTGGTTTGCGGCAGATTGCGCAGCACACAGAGTAACGCCTGGCGGCTGTAATCAAATGGGCTGGGCAACAGCAACGACTCGGCCT  >  NC_000913/1891694‑1891989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: