Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,255,992 A→T 6.6% K213I (AAA→ATA) ‡ yeiL → putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,255,9920AT 6.6% 526.2 / 19.5 152K213I (AAA→ATA) ‡yeiLputative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (71/70);  new base T (7/3);  total (78/74)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 167 total aligned reads displayed.

GAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCG  >  NC_000913/2255844‑2256140
                                                                                                                                                    |                                                                                                                                                    
gagTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaa                                                                                                                                                      >  3:610885/1‑149 (MQ=255)
 agTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                     <  2:1598401/149‑1 (MQ=255)
 agTATTTAGGCGTTTCTTATCGACATCTTCTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAACGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                     <  3:627236/149‑1 (MQ=255)
     tttAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtct                                                                                                                                                 >  2:278385/1‑149 (MQ=255)
      ttAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATtctc                                                                                                                                                <  2:1067651/149‑1 (MQ=255)
       tAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTcc                                                                                                                                               <  1:1227063/149‑1 (MQ=255)
       tAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTcc                                                                                                                                               <  4:1247349/149‑1 (MQ=255)
         ggCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgg                                                                                                                                             <  3:533117/149‑1 (MQ=255)
           cGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATTTATTCTCCgggt                                                                                                                                           <  1:435316/149‑2 (MQ=255)
            gTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGgat                                                                                                                                          >  3:1088827/1‑149 (MQ=255)
                   aTCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAg                                                                                                                                   >  4:1567244/1‑149 (MQ=255)
                    tCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGt                                                                                                                                  >  4:1617209/1‑149 (MQ=255)
                       aCATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTACTTAAAAGCAAGTAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGCaaa                                                                                                                               >  3:1082283/1‑149 (MQ=255)
                         aTCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGCGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa                                                                                                                             <  3:175944/149‑1 (MQ=255)
                         aTCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTaaaaa                                                                                                                             <  1:977878/149‑1 (MQ=255)
                            ttttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAatta                                                                                                                          >  3:1249621/1‑149 (MQ=255)
                             tttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAattat                                                                                                                         <  2:1579059/149‑1 (MQ=255)
                              ttATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAattatt                                                                                                                        <  1:814424/149‑1 (MQ=255)
                                atatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt                                                                                                                      <  2:1506671/149‑1 (MQ=255)
                                atatGTTCTCGCGCAGTTCATTCACGACAGTTTATTAATTAAAAGCAAGAAAGGGTATCTCAATAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAGATTCTCCGGGATGATGCAGTAAAAATTAtttt                                                                                                                      <  2:1554752/149‑1 (MQ=255)
                                 tatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAACGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGTAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                                                                     >  4:338588/1‑149 (MQ=255)
                                 tatGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTc                                                                                                                     <  2:96188/149‑1 (MQ=255)
                                   tGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                   >  1:1013630/1‑149 (MQ=255)
                                   tGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                   <  3:411324/149‑1 (MQ=255)
                                   tGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                   <  4:1317334/149‑1 (MQ=255)
                                    gTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAAt                                                                                                                  >  2:1031905/1‑149 (MQ=255)
                                     ttCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATa                                                                                                                 <  3:1367294/149‑1 (MQ=255)
                                      tctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATaaa                                                                                                                                                     >  3:404853/1‑112 (MQ=255)
                                       ctcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgc                                                                                                               >  2:772725/1‑149 (MQ=255)
                                        tcGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAgcg                                                                                                              >  3:184154/1‑149 (MQ=255)
                                          gcgcAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                                                                            >  2:1293260/1‑149 (MQ=255)
                                          gcgcAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGcgcg                                                                                                            >  1:1115600/1‑149 (MQ=255)
                                            gcAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGAt                                                                                                          >  1:68080/1‑149 (MQ=255)
                                                 tcattcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCg                                                                                                     >  3:351536/1‑149 (MQ=255)
                                                   attcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                   <  2:1566123/133‑1 (MQ=255)
                                                     tcaCGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATg                                                                                                 >  2:1687579/1‑149 (MQ=255)
                                                        cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCaa                                                                                              >  4:208020/1‑149 (MQ=255)
                                                        cgacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCaa                                                                                              >  4:424268/1‑149 (MQ=255)
                                                         gacgGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAAc                                                                                             <  1:613517/149‑1 (MQ=255)
                                                            ggTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt                                                                                          >  3:1203099/1‑149 (MQ=255)
                                                            ggTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCt                                                                                          >  3:581721/1‑149 (MQ=255)
                                                                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                                                                     >  4:1025519/1‑149 (MQ=255)
                                                                 attaattaAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATa                                                                                     <  1:895267/149‑1 (MQ=255)
                                                                    aattaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGt                                                                                  <  2:1699967/149‑1 (MQ=255)
                                                                     attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt                                                                                                                      >  4:1688809/1‑112 (MQ=255)
                                                                     attaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTAtttt                                                                                                                      <  3:1688809/112‑1 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCg                                                                                                     >  1:92064/1‑128 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                                                                                >  2:568988/1‑149 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                                                                                >  2:11010/1‑149 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGttt                                                                                >  3:1274422/1‑149 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                                                                                 <  2:1316146/148‑1 (MQ=255)
                                                                      ttaaAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGtt                                                                                 >  1:1316146/1‑148 (MQ=255)
                                                                         aaaGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTAt                                                                             <  1:1687579/149‑1 (MQ=255)
                                                                           aGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                                                                           <  4:172896/149‑1 (MQ=255)
                                                                              aaGAAAGGGTTTCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAAt                                                                                      >  4:409765/1‑135 (MQ=255)
                                                                               aGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTc                                                                       <  3:1567244/149‑1 (MQ=255)
                                                                                gAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                                                                      >  3:84625/1‑149 (MQ=255)
                                                                                gAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                                                                      >  3:1388545/1‑149 (MQ=255)
                                                                                gAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCt                                                                      <  4:585709/149‑1 (MQ=255)
                                                                                  aaGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTAc                                                                    <  4:331071/149‑1 (MQ=255)
                                                                                     ggTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACaaa                                                                 <  3:36453/149‑1 (MQ=255)
                                                                                      gTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAAt                                                                >  2:1712641/1‑149 (MQ=255)
                                                                                         tctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGa                                                             <  1:1712641/149‑1 (MQ=255)
                                                                                          ctcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAg                                                            <  4:1082283/149‑1 (MQ=255)
                                                                                           tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                           <  3:1352992/149‑1 (MQ=255)
                                                                                           tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                           >  4:1230289/1‑149 (MQ=255)
                                                                                           tcATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGc                                                           <  1:11010/149‑1 (MQ=255)
                                                                                             aTTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCAt                                                         >  3:891276/1‑149 (MQ=255)
                                                                                                aaaaaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTAc                                                      >  1:800305/1‑149 (MQ=255)
                                                                                                  aaaCAGAAAGCGGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGc                                                    <  4:62213/149‑1 (MQ=255)
                                                                                                   aaCAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTaa                                                                                       >  1:101281/1‑113 (MQ=255)
                                                                                                     cAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATc                                                                            <  2:817050/122‑1 (MQ=255)
                                                                                                      aGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGa                                                <  1:123906/149‑1 (MQ=255)
                                                                                                       gAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGAc                                               <  2:661033/149‑1 (MQ=255)
                                                                                                             aGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATaa                                         >  2:385841/1‑149 (MQ=255)
                                                                                                                    aGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCaa                                                                                                                   >  1:1167435/1‑68 (MQ=255)
                                                                                                                    aGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAAccc                                  >  4:1567054/1‑149 (MQ=255)
                                                                                                                     ggACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGAAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCg                                 <  4:657568/149‑1 (MQ=255)
                                                                                                                           gcgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATa                           <  4:361106/149‑1 (MQ=255)
                                                                                                                            cgcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAg                                       <  3:162164/136‑1 (MQ=255)
                                                                                                                             gcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAg                                                            <  4:560903/114‑1 (MQ=255)
                                                                                                                             gcTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGt                         >  2:851832/1‑149 (MQ=255)
                                                                                                                                 gagaTGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCa                     <  1:567459/149‑1 (MQ=255)
                                                                                                                                      ggACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcat                >  1:1570149/1‑149 (MQ=255)
                                                                                                                                       gACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCa                                                                                               >  2:1131229/1‑69 (MQ=255)
                                                                                                                                       gACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcatc               >  3:90925/1‑149 (MQ=255)
                                                                                                                                        tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCaa                                            >  1:1524635/2‑119 (MQ=255)
                                                                                                                                        tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCaa                                            <  2:1524635/118‑1 (MQ=255)
                                                                                                                                        tcccGGAGAATTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcatca              >  1:105159/2‑149 (MQ=255)
                                                                                                                                          ccGGAGAAGTTATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAgg            >  4:520108/1‑149 (MQ=255)
                                                                                                                                           cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                                                                           <  1:1440037/85‑1 (MQ=255)
                                                                                                                                           cGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATcc                                                                           >  2:1440037/1‑85 (MQ=255)
                                                                                                                                            ggAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGtt          >  2:281097/1‑149 (MQ=255)
                                                                                                                                            ggAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGtt          <  4:84625/149‑1 (MQ=255)
                                                                                                                                            ggAGAATAAATGCTCCGGGATGATGCAGTATAAATTATTTTCAATAGCGCGATTGCAGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGtt          <  4:1041875/149‑1 (MQ=255)
                                                                                                                                             gagaATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTg         <  4:90925/149‑1 (MQ=255)
                                                                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAAcc                                   <  3:749983/118‑1 (MQ=255)
                                                                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACtcatca              <  4:466771/139‑1 (MQ=255)
                                                                                                                                                  tAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAg    >  3:497948/1‑149 (MQ=255)
                                                                                                                                                    aaTTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCg  >  3:170803/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAGTTCATTCACGACGGTTTATTAATTAAAAGCAAGAAAGGGTATCTCATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAATTCTCCGGGATGATGCAGTAAAAATTATTTTCAATAGCGCGATTGCCGGATGCAACGCTTAATACGTTTTATCCGGTCTACAAATCGAGCATTACGCCAGACCAATAAAGAACCCGGCAATAGTCGCACTCATCAGGTTGGAAAGCG  >  NC_000913/2255844‑2256140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: