Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,315 C→G 8.0% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,3150CG 8.0% 557.1 / 32.7 163intergenic (+28/+75)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base C (78/71);  new base G (5/8);  total (84/79)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 167 total aligned reads displayed.

GCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACT  >  NC_000913/2286171‑2286462
                                                                                                                                                |                                                                                                                                                   
  aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttgg                                                                                                                                               <  1:226272/149‑1 (MQ=255)
  aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                     <  2:1393361/143‑1 (MQ=255)
  aaTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                     >  1:1393361/1‑143 (MQ=255)
   aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCTTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggt                                                                                                                                              <  1:1149150/149‑1 (MQ=255)
   aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                              <  3:1488752/149‑1 (MQ=255)
   aTCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACggttggt                                                                                                                                              <  4:66970/149‑1 (MQ=255)
    tCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                             <  4:930709/149‑1 (MQ=255)
       gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                          <  3:895122/149‑1 (MQ=255)
       gtgtGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                          <  3:1651611/149‑1 (MQ=255)
       gtatgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGttttt                                                                                                                                          <  3:527147/146‑1 (MQ=255)
        tgtgGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                         <  4:267128/149‑1 (MQ=255)
           ggAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttttttctt                                                                                                                                      <  1:517801/149‑4 (MQ=255)
               aaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCt                                                                                                                                  >  3:1021731/1‑149 (MQ=255)
               aaCGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCt                                                                                                                                  >  4:444176/1‑149 (MQ=255)
                  ggTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGGGTTGGttt                                                                                                                                            <  3:1260863/136‑1 (MQ=255)
                    tagtaTTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaat                                                                                                                             <  2:1182694/149‑1 (MQ=255)
                             cagcCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGAt                                                                                                                    <  1:1519296/149‑1 (MQ=255)
                                ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                                 >  3:22690/1‑149 (MQ=255)
                                ccACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATaaa                                                                                                                 >  1:852380/1‑149 (MQ=255)
                                 cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttt                                                                                                                                         <  3:1202786/124‑1 (MQ=255)
                                 cACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTGTATCTGCAATTAATTCGATAAAc                                                                                                                <  2:415370/149‑1 (MQ=255)
                                  aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTAc                                                                                                                                                     >  2:481711/1‑111 (MQ=255)
                                  aCGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAAc                                                                                                                >  2:1652866/1‑148 (MQ=255)
                                    gAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGa                                                                                                             >  4:841294/1‑149 (MQ=255)
                                    gAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGa                                                                                                             <  3:841294/149‑1 (MQ=255)
                                        cGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCg                                                                                                                      <  1:777189/136‑1 (MQ=255)
                                            aCGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGaca                                                                                                     >  3:1085271/1‑149 (MQ=255)
                                              gTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca                                                                                                   >  4:2912/1‑149 (MQ=255)
                                                         tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGTCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                        <  2:482179/149‑1 (MQ=255)
                                                         tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                        <  2:831464/149‑1 (MQ=255)
                                                         tGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt                                                                                        <  2:256570/149‑1 (MQ=255)
                                                           gTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGttt                                                                                      <  1:1084108/149‑1 (MQ=255)
                                                              gcgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtt                                                                                   <  2:914056/149‑1 (MQ=255)
                                                               cgcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAttt                                                                                  <  4:1562667/149‑1 (MQ=255)
                                                                gcACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtttt                                                                                 <  1:806840/149‑1 (MQ=255)
                                                                  aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGa                                                                                                       <  4:1662435/125‑1 (MQ=255)
                                                                  aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTAtt                                                                                   <  1:1664822/145‑1 (MQ=255)
                                                                  aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                               <  2:104017/149‑1 (MQ=255)
                                                                  aCCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                               <  4:1248080/149‑1 (MQ=255)
                                                                      tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                >  3:1629015/1‑144 (MQ=255)
                                                                      tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                <  4:1629015/144‑1 (MQ=255)
                                                                      tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                                                                           >  3:151992/1‑149 (MQ=255)
                                                                      tCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTa                                                                           <  2:1380083/149‑1 (MQ=255)
                                                                        aTGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCGTAAGggttggtt                                                                                                                                             >  1:1204537/1‑81 (MQ=38)
                                                                           gggTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGa                                                                      >  1:778580/1‑149 (MQ=255)
                                                                             atgtCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc                                                                    <  4:1522588/148‑1 (MQ=255)
                                                                               gtCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGt                                                                  >  2:1627517/1‑149 (MQ=255)
                                                                                  gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCa                                                               >  2:784506/1‑149 (MQ=255)
                                                                                  gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCa                                                               <  1:48885/149‑1 (MQ=255)
                                                                                     ggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGAc                                                            >  1:876442/1‑149 (MQ=255)
                                                                                       tCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCt                                                                               >  3:241209/1‑128 (MQ=255)
                                                                                           aGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc                                                                                            <  4:1616186/111‑1 (MQ=255)
                                                                                           aGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc                                                                                            >  3:1616186/1‑111 (MQ=255)
                                                                                             gTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCaaacttg                                                    >  3:1338444/1‑146 (MQ=255)
                                                                                              ttCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGc                                                   >  2:305855/1‑149 (MQ=255)
                                                                                                 aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                                                                     >  1:957415/1‑64 (MQ=255)
                                                                                                 aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTtata                                                                                                                                     <  2:957415/64‑1 (MQ=255)
                                                                                                 aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTg                                                >  2:1465705/1‑149 (MQ=255)
                                                                                                  aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                               <  4:1085271/149‑1 (MQ=255)
                                                                                                  aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                               >  1:455482/1‑149 (MQ=255)
                                                                                                  aaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGa                                               <  1:1616870/149‑1 (MQ=255)
                                                                                                     ccTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTa                                            >  2:1231915/1‑149 (MQ=255)
                                                                                                      ctctCGTGCCGACCAAAAATACCAAGAAAACACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAt                                           <  2:1116318/149‑1 (MQ=255)
                                                                                                       tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGAc                                                                                                            >  1:241289/1‑83 (MQ=255)
                                                                                                       tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                          >  4:1226906/1‑149 (MQ=255)
                                                                                                       tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                          >  4:467372/1‑149 (MQ=255)
                                                                                                       tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTAtt                                          >  2:1155044/1‑149 (MQ=255)
                                                                                                         tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTcc                                        >  1:1352001/1‑149 (MQ=255)
                                                                                                                gACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGa                                 >  1:1249309/1‑149 (MQ=255)
                                                                                                                    aaaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCt                             >  2:595105/1‑149 (MQ=255)
                                                                                                                     aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGAtt                               <  4:416511/146‑1 (MQ=255)
                                                                                                                      aaaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTAc                           >  2:849704/1‑149 (MQ=255)
                                                                                                                       aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCaattaa                                                                                                                          <  1:378244/53‑1 (MQ=255)
                                                                                                                       aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                                 <  2:1424233/126‑1 (MQ=255)
                                                                                                                       aaTCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCt                                                 >  1:1424233/1‑126 (MQ=255)
                                                                                                                           ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTg                      <  1:1168091/149‑1 (MQ=255)
                                                                                                                           ccAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTg                      >  1:1382122/1‑149 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTc                                                                                >  4:1314395/1‑90 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc                                                                    <  2:531844/102‑1 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAAc                                                                    >  1:531844/1‑102 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCa                                       >  3:455848/1‑131 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                     >  2:1591062/1‑149 (MQ=255)
                                                                                                                            cAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCACACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgc                     >  1:947103/1‑149 (MQ=255)
                                                                                                                             aaGAAAAAACCAACCCTTATGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTgcg                    >  3:992203/1‑149 (MQ=255)
                                                                                                                                aaaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGCGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCa                 >  4:707703/1‑149 (MQ=255)
                                                                                                                                 aaaaaCCAACCGTAAGGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAt                >  3:717918/1‑149 (MQ=255)
                                                                                                                                  aaaaCCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCAtt               <  1:1026508/149‑1 (MQ=255)
                                                                                                                                    aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATc                                                                                                 >  4:33500/1‑65 (MQ=255)
                                                                                                                                    aaccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATc                                                                                                 <  3:33500/65‑1 (MQ=255)
                                                                                                                                     accaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGcc            <  1:305855/149‑1 (MQ=255)
                                                                                                                                      ccaaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTCACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCa           >  1:1123550/1‑149 (MQ=255)
                                                                                                                                       caaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt          >  2:1718741/1‑149 (MQ=255)
                                                                                                                                       caaccCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCAt          <  4:20212/149‑1 (MQ=255)
                                                                                                                                         accCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAg        <  1:1627517/149‑1 (MQ=255)
                                                                                                                                          cccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACCGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGc       <  4:1021731/149‑1 (MQ=255)
                                                                                                                                           ccTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGct      >  2:686772/1‑149 (MQ=255)
                                                                                                                                            cTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctg     >  1:657922/1‑149 (MQ=255)
                                                                                                                                            cTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctg     >  3:450368/1‑149 (MQ=255)
                                                                                                                                            cTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctg     >  2:1364207/1‑149 (MQ=255)
                                                                                                                                             ttACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGctga    >  3:1671611/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAGAACGTCCAGACCACACCCGCCTGAGTATTCCACGGCGATTCTACCCCTGCGCCATTGCCATAGCTGACT  >  NC_000913/2286171‑2286462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: