Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,518,082 C→G 6.0% noncoding (35/76 nt) alaX ← tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,518,0820CG 6.0% 774.3 / 26.6 224noncoding (35/76 nt)alaXtRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base C (96/114);  new base G (3/11);  total (99/125)
Fisher's exact test for biased strand distribution p-value = 9.75e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCC  >  NC_000913/2517934‑2518229
                                                                                                                                                    |                                                                                                                                                   
ccAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgc                                                                                                                                                     >  3:1302285/1‑149 (MQ=255)
   atgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccat                                                                                                                                                  >  3:395269/1‑149 (MQ=255)
   atgatgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccat                                                                                                                                                  <  1:397425/149‑1 (MQ=255)
       tgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCCGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCaa                                                                                                                                              <  3:367235/149‑1 (MQ=255)
       tgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCaa                                                                                                                                              <  1:1582540/149‑1 (MQ=255)
        gCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAg                                                                                                                                             >  4:1621500/1‑149 (MQ=255)
         cTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCTGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGGCATGCAAga                                                                                                                                            <  2:1181475/149‑2 (MQ=255)
         cTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGGCATGCAAga                                                                                                                                            <  3:1032887/149‑2 (MQ=255)
         cTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAAACCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                                                                                            >  2:106051/1‑149 (MQ=255)
          tttCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGGCATGCAAgag                                                                                                                                           <  1:1526534/149‑3 (MQ=255)
              aCCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctct                                                                                                                                       >  1:287582/1‑149 (MQ=255)
                cAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTcc                                                                                                                                     >  3:34819/1‑149 (MQ=255)
                      gcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcca                                                                                                                                                   <  4:679871/129‑1 (MQ=255)
                         cTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGc                                                                                                                            <  3:1559313/149‑1 (MQ=255)
                          tAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCt                                                                                                                           <  1:1495668/149‑1 (MQ=255)
                              ccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAg                                                                                                                       >  2:1313128/1‑149 (MQ=255)
                                         cTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTAGCAGCGAGGGTTGGAAACTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGc                                                                                                            <  4:1006380/149‑1 (MQ=255)
                                          tGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCg                                                                                                           >  3:690928/1‑149 (MQ=255)
                                          tGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCg                                                                                                           >  2:895083/1‑149 (MQ=255)
                                          tGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCg                                                                                                           >  4:774413/1‑149 (MQ=255)
                                             aTTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                                                                                        >  1:333946/1‑149 (MQ=255)
                                             aTTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                                                                                        >  2:1539591/1‑149 (MQ=255)
                                              ttACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGcatata                                                                                                       >  2:65563/1‑144 (MQ=255)
                                                 cAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGt                                                                                                    <  3:871269/149‑1 (MQ=255)
                                                  aaaaGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTa                                                                                                   <  1:486209/149‑1 (MQ=255)
                                                   aaaGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTAc                                                                                                  >  3:48006/1‑149 (MQ=255)
                                                           aaaaaGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTg                                                                                          >  1:1097701/1‑149 (MQ=255)
                                                             aaaGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCt                                                                                        <  1:1639486/149‑1 (MQ=255)
                                                               aGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTgg                                                                                      <  3:1005197/149‑1 (MQ=255)
                                                               aGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTgg                                                                                      >  3:801887/1‑149 (MQ=255)
                                                                gCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgc                                                                                                                                                     >  3:1608536/1‑85 (MQ=255)
                                                                 cAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCACTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGt                                                                                    <  2:1526230/149‑1 (MQ=255)
                                                                       cAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaaa                                                                              <  2:496404/149‑1 (MQ=255)
                                                                        aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc                                                                                                                 >  1:1085063/1‑113 (MQ=255)
                                                                        aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc                                                                                                                 <  2:1085063/113‑1 (MQ=255)
                                                                        aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCaa                                                                                               <  1:826751/131‑1 (MQ=255)
                                                                        aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAt                                                                             >  2:171086/1‑149 (MQ=255)
                                                                        aaaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAt                                                                             >  4:175523/1‑149 (MQ=255)
                                                                        aaaaaaCCCCCACTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAt                                                                             >  1:1274521/1‑149 (MQ=255)
                                                                         aaaaaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCtaatat                                                                            >  2:1445545/1‑146 (MQ=255)
                                                                          aaaaCCCCCGCTTTGCAGCGAGGGTTGAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAttt                                                                           <  3:1401327/149‑1 (MQ=255)
                                                                            aaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg                                                                         <  4:587100/149‑1 (MQ=255)
                                                                            aaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg                                                                         <  2:358874/149‑1 (MQ=255)
                                                                            aaCCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCCGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg                                                                         <  2:1112878/149‑1 (MQ=255)
                                                                              cccccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaa                                                                                >  1:542280/1‑140 (MQ=255)
                                                                                cccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                                     <  4:926557/149‑1 (MQ=255)
                                                                                cccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                                     >  3:1368111/1‑149 (MQ=255)
                                                                                cccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                                     >  2:544781/1‑149 (MQ=255)
                                                                                 ccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCtat                                                                                                                         <  1:1359333/96‑1 (MQ=255)
                                                                                 ccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg                                                                    >  4:1084378/1‑149 (MQ=255)
                                                                                 ccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg                                                                    >  4:537877/1‑149 (MQ=255)
                                                                                 ccGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg                                                                    <  1:950075/149‑1 (MQ=255)
                                                                                     tttGCAGCGAGGGTTGGAAATTTGGTGGCGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGGCATGCAAga                                                                                                                                            >  3:664494/1‑72 (MQ=255)
                                                                                     tttGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCACGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTaa                                                                <  4:1183734/149‑1 (MQ=255)
                                                                                       tGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGc                                                              <  2:1124440/149‑1 (MQ=255)
                                                                                        gCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCCCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCg                                                             <  2:96992/149‑1 (MQ=255)
                                                                                          aGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCggg                                                           <  3:1497861/149‑1 (MQ=255)
                                                                                           gCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGa                                                          <  1:1407575/149‑1 (MQ=255)
                                                                                           gCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGa                                                          <  4:1606803/149‑1 (MQ=255)
                                                                                              aGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgc                                                                                                                                                     <  2:586746/55‑1 (MQ=255)
                                                                                                         tttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGTGTTTACTTACGAAGTTTGCTGGGAGCAAAATTTGGTGGAGCTGAGCGGGATCGACCAGCTGAcc                                            >  4:1466448/1‑149 (MQ=21)
                                                                                                         tttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAcc                                            <  3:1131918/149‑1 (MQ=255)
                                                                                                         tttGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAcc                                            >  2:139925/1‑149 (MQ=255)
                                                                                                             gtggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCtt                                        <  4:1302285/149‑1 (MQ=255)
                                                                                                             gtggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCtt                                        <  3:1055572/149‑1 (MQ=255)
                                                                                                             gtggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCtt                                        <  3:175523/149‑1 (MQ=255)
                                                                                                               ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg                                                                         >  1:122760/1‑114 (MQ=255)
                                                                                                               ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                                              <  4:1577181/141‑1 (MQ=255)
                                                                                                               ggAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGa                                              >  3:1577181/1‑141 (MQ=255)
                                                                                                                   cTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgc                                  <  2:1274521/149‑1 (MQ=255)
                                                                                                                    tAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc                                 <  4:1368111/149‑1 (MQ=255)
                                                                                                                      aGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACTTACCAAGTTTGCTGGGTGCAAAATTTGGTGCAGCTAAACTGGGTCGAACCGCTGTCCTCTTGcataccat                               >  4:1653848/1‑149 (MQ=25)
                                                                                                                       gCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatg                              >  3:1007893/1‑149 (MQ=255)
                                                                                                                          ggATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtg                                                                          >  3:768636/1‑102 (MQ=255)
                                                                                                                            aTCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGa                                                                     <  2:251745/105‑1 (MQ=255)
                                                                                                                             tCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGGCATGCAAgag                        <  4:751180/149‑3 (MQ=255)
                                                                                                                             tCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCGCTTGCATGGCATGCAAgag                        <  4:1274135/149‑3 (MQ=255)
                                                                                                                                aaCCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctc                     >  1:989344/1‑149 (MQ=255)
                                                                                                                                   cGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACTGCTGACCTCTTGcatgc                                  >  1:1458591/1‑133 (MQ=255)
                                                                                                                                        aCCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                         >  1:17050/1‑137 (MQ=255)
                                                                                                                                         gcgctTGCATGGCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAACTTGGTGGAGCTAAGCGGGATCGAACCGCTGACATCTTGCATGCCATGCAAGCGCTCTCCCAGCTg            >  2:1468814/4‑149 (MQ=255)
                                                                                                                                          ctctTGCATGGCATGCAAGCGCTTTCCCAGCTCAGCAATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           <  2:919749/149‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGtt                                                                                            <  2:552997/68‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGtt                                                                                            >  1:552997/1‑68 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGAt                                                         <  1:800346/103‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           >  3:1598169/1‑149 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           <  1:739509/149‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           <  3:235636/149‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           <  4:370743/149‑1 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAATTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCCCTCTCCCAGCTGa           >  2:227400/1‑149 (MQ=255)
                                                                                                                                          ctctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACCATGCGATTATGTACCAAGTTTGCTGGGTGCAAAAGTGGATGGAGCTAAGCTGGATCGAACCGCTGACCTCTTGCATGCCATCCAAGCGCTCTCCCAGCTGa           >  3:285395/1‑149 (MQ=37)
                                                                                                                                          ctctTGCATGCCATGAAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGa           <  2:785766/149‑1 (MQ=255)
                                                                                                                                           tctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCaa                           <  4:908017/132‑1 (MQ=255)
                                                                                                                                           tctTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAg          >  4:1531917/1‑149 (MQ=255)
                                                                                                                                             ttGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCt        >  1:23741/1‑149 (MQ=255)
                                                                                                                                               gCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCtat      >  4:286829/1‑149 (MQ=255)
                                                                                                                                                  tgccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGc   >  1:1062410/1‑149 (MQ=255)
                                                                                                                                                   gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTAc                                                                                                      >  3:1194758/1‑49 (MQ=255)
                                                                                                                                                   gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTAc                                                                                                      <  4:1194758/49‑1 (MQ=255)
                                                                                                                                                   gccatgcAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGcc  >  3:951616/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCC  >  NC_000913/2517934‑2518229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: