Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,620,218 T→A 5.1% intergenic (‑58/+28) uraA ← / ← upp uracil permease/uracil phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,620,2180TA 5.1% 487.6 / 12.3 137intergenic (‑58/+28)uraA/uppuracil permease/uracil phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (71/59);  new base A (3/4);  total (74/63)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 143 total aligned reads displayed.

GCACCAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCC  >  NC_000913/2620069‑2620366
                                                                                                                                                      |                                                                                                                                                     
gCACCAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAGCCGACTCtt                                                                                                                                                       <  2:1322380/149‑1 (MQ=255)
 cACCAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTAATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCtt                                                                                                                                                       >  3:1168190/1‑149 (MQ=255)
          tGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGc                                                                                                                                             <  2:888689/149‑1 (MQ=255)
            gCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATTGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCtt                                                                                                                                           <  3:1175244/149‑1 (MQ=255)
            gCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGCtt                                                                                                                                           <  4:1250729/149‑1 (MQ=255)
              gAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTa                                                                                                                                         >  3:354924/1‑149 (MQ=255)
               aaCAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGCttttt                                                                                                                                        <  2:810419/149‑3 (MQ=255)
               aaCAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGCttttt                                                                                                                                        <  1:1297723/149‑3 (MQ=255)
                aCAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAat                                                                                                                                       >  1:552054/1‑149 (MQ=255)
                aCAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAat                                                                                                                                       >  3:14663/1‑149 (MQ=255)
                 cAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAatt                                                                                                                                      >  1:638638/1‑149 (MQ=255)
                 cAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAatt                                                                                                                                      >  3:810121/1‑149 (MQ=255)
                  aaaTGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAatta                                                                                                                                     <  3:1457177/149‑1 (MQ=255)
                   aaTGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAattat                                                                                                                                    >  2:991802/1‑149 (MQ=255)
                    aTGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAattatt                                                                                                                                   <  3:194820/149‑1 (MQ=255)
                       ttGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTAttttt                                                                                                                                >  1:1366314/1‑149 (MQ=255)
                        tGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTa                                                                                                                               <  2:249390/149‑1 (MQ=255)
                           aaaCTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTCTTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTc                                                                                                                                                >  3:587532/1‑129 (MQ=255)
                           aaaCTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTc                                                                                                                                                <  4:587532/129‑1 (MQ=255)
                                      gATTGTCTGTAAAAGTGGCGGTTTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                                 <  2:833374/149‑1 (MQ=255)
                                      gATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                                 >  1:1361756/1‑149 (MQ=255)
                                      gATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                                 <  4:14663/149‑1 (MQ=255)
                                           tCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCaaa                                                                                                            >  2:173543/1‑149 (MQ=255)
                                              gTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTa                                                                                                                               >  2:1477380/1‑127 (MQ=255)
                                                aaaaGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGAttt                                                                                                       <  1:107825/149‑1 (MQ=255)
                                                  aaGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTg                                                                                                     >  2:785835/1‑149 (MQ=255)
                                                    gTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTc                                                                                                   <  1:147513/149‑1 (MQ=255)
                                                         ggTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCgg                                                                                              >  4:564741/1‑149 (MQ=255)
                                                         ggTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCgg                                                                                              >  4:519389/1‑149 (MQ=255)
                                                             tttCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATc                                                                                          <  3:1291570/149‑1 (MQ=255)
                                                                 actcacCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCgg                                                                                                                                              <  3:568704/93‑1 (MQ=255)
                                                                 actcacCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCg                                                                                      >  4:868019/1‑149 (MQ=255)
                                                                  ctcacCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTTTAAGAGTCGGc                                                                                                                                             <  1:1447190/93‑1 (MQ=255)
                                                                     acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                                 <  2:458671/118‑1 (MQ=255)
                                                                     acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTa                                                                                                                 >  1:458671/1‑118 (MQ=255)
                                                                     acCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGc                                                                                  >  3:1677450/1‑149 (MQ=255)
                                                                       cccGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGccc                                                                                >  3:498884/1‑149 (MQ=255)
                                                                         cGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCg                                                                               <  2:1696809/149‑1 (MQ=255)
                                                                          gATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCgca                                                                             <  2:1366314/149‑3 (MQ=255)
                                                                              gCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataa                                                                         <  4:882748/149‑1 (MQ=255)
                                                                               cACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                        >  3:887946/1‑149 (MQ=255)
                                                                               cACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                        <  3:563489/149‑1 (MQ=255)
                                                                               cACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                        <  1:1685933/149‑1 (MQ=255)
                                                                                  ggCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATc                                                                                          <  1:941899/128‑1 (MQ=255)
                                                                                   gcgcGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTAt                                                                    <  1:785835/149‑1 (MQ=255)
                                                                                     gcgTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt                                                                                                                              >  4:1289529/1‑89 (MQ=255)
                                                                                     gcgTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTAt                                                                                                                              <  3:1289529/89‑1 (MQ=255)
                                                                                     gcgTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATcc                                                                  <  2:632738/149‑1 (MQ=255)
                                                                                       gTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGt                                                                >  4:678978/1‑149 (MQ=255)
                                                                                                  atCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGt                                                     <  1:306096/149‑1 (MQ=255)
                                                                                                   tCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTc                                                    >  2:271962/1‑149 (MQ=255)
                                                                                                    ccTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTcc                                                   <  4:1366127/149‑1 (MQ=255)
                                                                                                     ctctGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTccc                                                  >  1:1544103/1‑149 (MQ=255)
                                                                                                      tctGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTTAAGTCGGCTTTAATTATTTTTATTCTTTATCTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 >  4:1660563/1‑149 (MQ=255)
                                                                                                      tctGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 <  2:897774/149‑1 (MQ=255)
                                                                                                      tctGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 >  1:202823/1‑149 (MQ=255)
                                                                                                      tctGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 >  1:1078349/1‑149 (MQ=255)
                                                                                                        tGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGa                                               <  3:1299244/149‑1 (MQ=255)
                                                                                                        tGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGa                                               <  4:354924/149‑1 (MQ=255)
                                                                                                        tGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGa                                               <  1:465791/149‑1 (MQ=255)
                                                                                                         gTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                        >  2:1503133/1‑123 (MQ=255)
                                                                                                         gTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGaataat                                                                        <  1:1503133/123‑1 (MQ=255)
                                                                                                          tattatGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCa                                                       <  3:378570/139‑1 (MQ=255)
                                                                                                                gtgtTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCa                                                       <  4:1688772/133‑1 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAGGATTTTGTCACCGGCATCGCCGATGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCAATGc                                    >  3:283589/1‑149 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  4:1015052/149‑1 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  1:991802/149‑1 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    >  3:48296/1‑149 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  2:1599410/149‑1 (MQ=255)
                                                                                                                   ttATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  4:439690/149‑1 (MQ=255)
                                                                                                                       aGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGta                                >  4:1002139/1‑149 (MQ=255)
                                                                                                                        ggCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGtat                               >  2:1206402/1‑149 (MQ=255)
                                                                                                                         gcgcTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCTAGTCCCGGAATAATGTATCCTTGCTCGTTCAGTCCCTGATCAATTGATGTGGtata                              >  4:1200181/1‑149 (MQ=255)
                                                                                                                         gcgcTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGtata                              <  1:418137/149‑1 (MQ=255)
                                                                                                                         gcgcTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTGATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGtata                              <  2:352074/149‑1 (MQ=255)
                                                                                                                             tttACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGt                          <  1:818118/149‑1 (MQ=255)
                                                                                                                               tACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTc                        <  1:1206402/149‑1 (MQ=255)
                                                                                                                                  tCAAAAAAAAGCCGGCTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGt                                                                      <  1:1282821/100‑1 (MQ=255)
                                                                                                                                  tCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTTACCGGCATCGCCGAGGCCCGGAATAATGt                                                                      >  2:1282821/1‑100 (MQ=255)
                                                                                                                                  tCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGAc                     >  1:1038588/1‑149 (MQ=255)
                                                                                                                                   cAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  2:445022/133‑1 (MQ=255)
                                                                                                                                     taattaaaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGt                   >  3:882706/6‑149 (MQ=255)
                                                                                                                                     aaaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 >  1:1174901/1‑119 (MQ=255)
                                                                                                                                     aaaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCt                                                 <  2:1174901/119‑1 (MQ=255)
                                                                                                                                     aaaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGt                   >  1:1165464/1‑149 (MQ=255)
                                                                                                                                     aaaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGt                   >  2:1173673/1‑149 (MQ=255)
                                                                                                                                     aaaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGt                   >  4:690250/1‑149 (MQ=255)
                                                                                                                                      aaaaaaaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTc                  >  3:555047/1‑149 (MQ=255)
                                                                                                                                         taaaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCgg               >  4:1340660/2‑149 (MQ=255)
                                                                                                                                          aaaGCCGACTTTAAGAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCggg              >  2:426371/1‑149 (MQ=255)
                                                                                                                                           aaGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCAGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGt             <  4:1168190/149‑1 (MQ=255)
                                                                                                                                              ccGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTAACCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTgcg          >  3:1596705/1‑149 (MQ=255)
                                                                                                                                                gACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTcc                 >  4:1069228/1‑140 (MQ=255)
                                                                                                                                                gACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTcc                 <  3:1069228/140‑1 (MQ=255)
                                                                                                                                                gACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCt        >  1:834946/1‑149 (MQ=255)
                                                                                                                                                   tctTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCtttt     <  2:1165464/149‑1 (MQ=255)
                                                                                                                                                   tctTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGATGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCACTGATCAACCAATGCGGTCTACAGTTCGACGTCCGGGTGCGCtttt     >  4:602123/1‑149 (MQ=255)
                                                                                                                                                     ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGc                                    <  3:214161/116‑1 (MQ=255)
                                                                                                                                                     ttAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTc   <  4:249173/149‑1 (MQ=255)
                                                                                                                                                      tAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTcc  <  1:1626193/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCACCAAACATGGCGAACAAATGTTGCAAACTAAGCGGGATTGTCTGTAAAAGTGGCGGTCTTTCACTCACCCCGATAGCACGGCGCGTCATAGTATTATCCTCTGTATTATGTGTTATAGGCGCTTTACTCAAAAAAAAGCCGACTCTTAAAGTCGGCTTTAATTATTTTTATTCTTTATTTCGTACCAAAGATTTTGTCACCGGCATCGCCGAGGCCCGGAATAATGTATCCGTGCTCGTTCAGTCCCTGATCAATCGATGCGGTATACAGTTCGACGTCCGGGTGCGCTTTTTCC  >  NC_000913/2620069‑2620366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: