Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,630,919 G→A 5.7% intergenic (+54/+39) yfgI → / ← guaA nalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,630,9190GA 5.7% 519.7 / 14.3 158intergenic (+54/+39)yfgI/guaAnalidixic acid resistance protein, putative periplasmic protein/GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base G (73/76);  new base A (3/6);  total (76/82)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

Only 100 of 165 total aligned reads displayed.

CTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATC  >  NC_000913/2630771‑2631065
                                                                                                                                                    |                                                                                                                                                  
     gCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                               >  1:1485081/1‑149 (MQ=255)
     gCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACa                                                                                                                                               >  2:1284548/1‑149 (MQ=255)
       cAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACaga                                                                                                                                             <  4:357256/149‑1 (MQ=255)
         aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAgg                                                                                                                                           >  3:1219549/1‑149 (MQ=255)
         aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAgg                                                                                                                                           <  1:260772/149‑1 (MQ=255)
          cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAggg                                                                                                                                          <  1:532597/149‑1 (MQ=255)
          cAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAggg                                                                                                                                          <  3:946910/149‑1 (MQ=255)
           aGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGt                                                                                                                                         >  3:1428770/1‑149 (MQ=255)
             aaaTATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGttt                                                                                                                                       <  2:834966/149‑1 (MQ=255)
             aaaTATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGttt                                                                                                                                       <  1:672033/149‑1 (MQ=255)
               atatGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGtttt                                                                                                                                      >  2:250827/1‑148 (MQ=255)
                 atGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGtt                                                                                                                                   <  4:1415047/149‑1 (MQ=255)
                    aCTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcat                                                                                                                                <  1:1205161/149‑1 (MQ=255)
                      tGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGttcattc                                                                                                                              >  2:1511120/1‑149 (MQ=255)
                        aaGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTtcattcat                                                                                                                            >  1:576729/1‑149 (MQ=255)
                         aGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATa                                                                                                                           <  4:476345/149‑1 (MQ=255)
                            cGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTg                                                                                                                        >  3:916395/1‑149 (MQ=255)
                               tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACagag                                                                                                                                            >  3:800413/1‑126 (MQ=255)
                               tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAg                                                                                                                     <  3:1628438/149‑1 (MQ=255)
                                cgcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAgg                                                                                                                    <  1:649143/149‑1 (MQ=255)
                                cgcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAgg                                                                                                                    <  1:840293/149‑1 (MQ=255)
                                   gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTaa                                                                                                                                                 >  4:396379/1‑117 (MQ=255)
                                   gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTaa                                                                                                                                                 <  3:396379/117‑1 (MQ=255)
                                      cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              <  1:561260/149‑1 (MQ=255)
                                      cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              <  1:1698098/149‑1 (MQ=255)
                                        gatgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATc                                                                                                            <  1:695892/149‑1 (MQ=255)
                                         atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCa                                                                                                                      <  3:1227066/138‑1 (MQ=255)
                                         atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCa                                                                                                                      >  4:1227066/1‑138 (MQ=255)
                                           gaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAtt                                                                                                         <  3:38372/149‑1 (MQ=255)
                                           gaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCAtt                                                                                                         <  2:1526832/149‑1 (MQ=255)
                                            aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              <  2:166484/143‑1 (MQ=255)
                                            aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              >  1:166484/1‑143 (MQ=255)
                                            aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTc                                                                                                        <  1:1573482/149‑1 (MQ=255)
                                            aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTc                                                                                                        <  4:502716/149‑1 (MQ=255)
                                              aaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTaa                                                                                                                                                 >  3:873190/1‑106 (MQ=255)
                                               aGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCa                                                                                                     <  4:1428770/149‑1 (MQ=255)
                                                gCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCAc                                                                                                    >  2:981345/1‑149 (MQ=255)
                                                  gTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAggg                                                                                                                                          <  1:796266/109‑1 (MQ=255)
                                                     ggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAAt                                                                                               >  2:1465504/1‑149 (MQ=255)
                                                     ggCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAAt                                                                                               >  2:1017256/1‑149 (MQ=255)
                                                      gCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATg                                                                                              <  4:1588964/149‑1 (MQ=255)
                                                        aaaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGt                                                                                            >  2:649809/1‑149 (MQ=255)
                                                        aaaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGt                                                                                            >  1:934664/1‑149 (MQ=255)
                                                         aaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTa                                                                                           >  1:1307560/1‑149 (MQ=255)
                                                         aaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTa                                                                                           <  1:954870/149‑1 (MQ=255)
                                                               aagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTg                                                                                                                                     <  2:946731/101‑1 (MQ=255)
                                                               aagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTggc                                                                                     <  4:766890/149‑1 (MQ=255)
                                                                 gagaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGtttt                                                                                                                                      <  2:396295/98‑1 (MQ=38)
                                                                 gagaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTTACTACAGAGGGtttt                                                                                                                                      >  1:396295/1‑98 (MQ=38)
                                                                    aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGt                                                                                                                                    <  3:129623/97‑1 (MQ=255)
                                                                      cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGc                                                                              <  4:916395/149‑1 (MQ=255)
                                                                      cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGc                                                                              <  4:812054/149‑1 (MQ=255)
                                                                       aaaTCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGcc                                                                             >  4:1517756/1‑149 (MQ=255)
                                                                            ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGa                                                                        <  3:1466836/149‑1 (MQ=255)
                                                                            ggACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGa                                                                        <  4:569979/149‑1 (MQ=255)
                                                                             gACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGAt                                                                       <  1:176430/149‑1 (MQ=255)
                                                                                aGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              <  4:1505196/107‑1 (MQ=255)
                                                                                aGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCaaa                                                                                                              >  3:1505196/1‑107 (MQ=255)
                                                                                  cTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGtt                                                                                                                                   <  4:487206/84‑1 (MQ=255)
                                                                                  cTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGtt                                                                                                                                   >  3:487206/1‑84 (MQ=255)
                                                                                   tGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 <  3:240814/149‑1 (MQ=255)
                                                                                      tttAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGt                                                                     <  2:696806/142‑1 (MQ=255)
                                                                                       ttaattaaTCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATAcacc                                                             >  1:698049/1‑149 (MQ=255)
                                                                                          attaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAcg                                                          >  1:728961/1‑149 (MQ=255)
                                                                                           ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGttt                                                                                                                                       >  4:416078/1‑71 (MQ=255)
                                                                                           ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGttt                                                                                                                                       <  3:416078/71‑1 (MQ=255)
                                                                                           ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAgg                                                                                                                    <  4:1191408/90‑1 (MQ=255)
                                                                                           ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAgg                                                                                                                    >  3:1191408/1‑90 (MQ=255)
                                                                                           ttaatCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCAcgc                                                         >  1:101339/1‑149 (MQ=255)
                                                                                                    gTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATAc                                                >  2:1691890/1‑149 (MQ=255)
                                                                                                        gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATaca                                                               >  1:1053936/1‑130 (MQ=255)
                                                                                                        gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATaca                                                               <  2:1053936/130‑1 (MQ=255)
                                                                                                        gTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATTCCGtt                                            <  4:212465/149‑1 (MQ=255)
                                                                                                            cTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACt                                        <  4:732194/149‑1 (MQ=255)
                                                                                                             ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  1:1118366/1‑149 (MQ=255)
                                                                                                             ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  2:872105/1‑149 (MQ=255)
                                                                                                             ttCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACtt                                       >  2:1009351/1‑149 (MQ=255)
                                                                                                                aaTCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCAt                                    <  2:1307560/149‑1 (MQ=255)
                                                                                                                  tCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 <  1:33109/118‑1 (MQ=255)
                                                                                                                  tCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 >  2:33109/1‑118 (MQ=255)
                                                                                                                   ccTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTGCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATAcacca                                                            >  3:1145204/1‑122 (MQ=255)
                                                                                                                   ccTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATAcacca                                                            <  4:1145204/122‑1 (MQ=255)
                                                                                                                    cTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGAt                                >  4:793312/1‑149 (MQ=255)
                                                                                                                      ataATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATaa                              <  3:74483/149‑1 (MQ=255)
                                                                                                                      ataATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATaa                              >  4:1485553/1‑149 (MQ=255)
                                                                                                                             ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       <  4:1631865/149‑1 (MQ=255)
                                                                                                                             ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       <  2:645830/149‑1 (MQ=255)
                                                                                                                             ttGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGtt                       <  1:15829/149‑1 (MQ=255)
                                                                                                                                aaGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 >  3:75328/1‑104 (MQ=255)
                                                                                                                                aaGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATa                                                                 <  4:75328/104‑1 (MQ=255)
                                                                                                                                  gATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGaaa                  >  1:138321/1‑149 (MQ=255)
                                                                                                                                       aaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGa                                 >  4:806422/1‑129 (MQ=255)
                                                                                                                                       aaaaCCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGa             >  3:241359/1‑149 (MQ=255)
                                                                                                                                         aaCCCTCTGTTACTACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGAcc           >  3:1657521/1‑149 (MQ=255)
                                                                                                                                           cccTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGa         <  3:444391/149‑1 (MQ=255)
                                                                                                                                             ctctGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAgg       >  3:1628763/1‑149 (MQ=255)
                                                                                                                                             ctctGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAgg       <  2:1700695/149‑1 (MQ=255)
                                                                                                                                                 gtagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAAt   <  1:1009351/149‑1 (MQ=255)
                                                                                                                                                  tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAAt                             >  4:527698/1‑122 (MQ=255)
                                                                                                                                                  tagtaACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAAt                             <  3:527698/122‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CTGACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACCCTCTGTAGTAACAGAGGGTTTTGTTCATTCATAGTGCAGGGTCAAATCATTCCCACTCAATGGTAGCTGGCGGCTTGCCGCTGATGTCATACACCACGCGGGAAATACCGTTCACTTCATTGATAATGCGGTTGGAAACGCGACCGAGGAAATC  >  NC_000913/2630771‑2631065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: