Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,672,640 G→T 6.0% Q198H (CAG→CAT) ‡ hcaD → phenylpropionate dioxygenase, ferredoxin reductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,672,6400GT 6.0% 588.2 / 19.4 166Q198H (CAG→CAT) ‡hcaDphenylpropionate dioxygenase, ferredoxin reductase subunit
Reads supporting (aligned to +/- strand):  ref base G (79/77);  new base T (6/4);  total (85/81)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 170 total aligned reads displayed.

GGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCG  >  NC_000913/2672497‑2672788
                                                                                                                                                |                                                                                                                                                    
ggTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTgg                                                                                                                                                 >  3:790756/1‑149 (MQ=255)
     cGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTgc                                                                                                                                            <  3:826302/149‑1 (MQ=255)
      ggAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTgcg                                                                                                                                           >  2:48120/1‑149 (MQ=255)
       gAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCACACCAGCCTGCTGgtggcgc                                                                                                                                           <  2:256469/149‑5 (MQ=255)
           tATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTc                                                                                                                                      >  1:358762/1‑149 (MQ=255)
             ttGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTgc                                                                                                                                            >  1:1626471/1‑141 (MQ=255)
              tGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGc                                                                                                                                   >  2:1629111/1‑149 (MQ=255)
              tGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGc                                                                                                                                   <  4:790756/149‑1 (MQ=255)
                 tCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCa                                                                                                                                >  1:1603887/1‑149 (MQ=255)
                     gAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGATCaataa                                                                                                                            <  2:919123/149‑1 (MQ=255)
                      aaCTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataat                                                                                                                           <  3:1033460/149‑1 (MQ=255)
                         tGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGcc                                                                                                                        <  1:48120/149‑1 (MQ=255)
                          ggCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCa                                                                                                                       >  2:1291031/1‑149 (MQ=255)
                           gCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCAt                                                                                                                      <  3:326572/149‑1 (MQ=255)
                           gCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCGCAATAATGCCAt                                                                                                                      <  2:586355/149‑1 (MQ=255)
                               ccAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGaa                                                                                                                  >  3:1542617/1‑149 (MQ=255)
                               ccAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGaa                                                                                                                  <  2:1537973/149‑1 (MQ=255)
                                 aGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAAc                                                                                                                 >  1:439408/1‑148 (MQ=255)
                                       aCGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGACAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGt                                                                                                          >  2:427545/1‑149 (MQ=255)
                                            gcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATg                                                                                                     <  1:1669847/149‑1 (MQ=255)
                                            gcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATg                                                                                                     <  4:711071/149‑1 (MQ=255)
                                            gcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATg                                                                                                     <  2:1168212/149‑1 (MQ=255)
                                            gcgcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATg                                                                                                     <  3:1676149/149‑1 (MQ=255)
                                              gcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGt                                                                                                   <  3:915661/149‑1 (MQ=255)
                                              gcagATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGt                                                                                                   >  1:1463973/1‑149 (MQ=255)
                                                   tGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGt                                                                                                   <  1:1329776/144‑1 (MQ=255)
                                                   tGTAAGGTGACAGTGATTGAACTCGCGGCAACCGTCATGGGCCGTAATGCACCCACGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTATGCTCAATAATGCCATTGAACATGTGGTCGCTGGTGaaaa                                                                                              >  3:1447786/1‑149 (MQ=255)
                                                    gTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGaaaaa                                                                                             <  1:1274613/149‑1 (MQ=255)
                                                    gTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGGGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGACGGTGaaaaa                                                                                             <  2:1653606/149‑1 (MQ=255)
                                                     tAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAg                                                                                            >  1:1694951/1‑149 (MQ=255)
                                                          tGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGaa                                                                                       >  2:1710981/1‑149 (MQ=255)
                                                          tGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGaa                                                                                       <  2:1099800/149‑1 (MQ=255)
                                                          tGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGaa                                                                                       <  3:1671868/149‑1 (MQ=255)
                                                              aGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCGATCTTTTACAGCGCCACCAGCAGGCTTGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGa                                                                                   >  2:985574/1‑149 (MQ=255)
                                                               gTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGAc                                                                                  <  4:1542617/149‑1 (MQ=255)
                                                                tGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACg                                                                                 >  4:981105/1‑149 (MQ=255)
                                                                 gATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGc                                                                                <  3:317373/149‑1 (MQ=255)
                                                                  aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACAt                                                                                                               <  2:226363/117‑1 (MQ=255)
                                                                  aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCt                                                                               >  2:1044838/1‑149 (MQ=255)
                                                                  aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCt                                                                               <  1:855705/149‑1 (MQ=255)
                                                                  aTTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATGGAACAt                                                                                                               >  1:226363/1‑117 (MQ=255)
                                                                   ttGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataa                                                                                                                            >  3:1317689/1‑103 (MQ=255)
                                                                   ttGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCaataa                                                                                                                            <  4:1317689/103‑1 (MQ=255)
                                                                   ttGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                              >  2:1572052/1‑149 (MQ=255)
                                                                   ttGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTg                                                                              >  2:910193/1‑149 (MQ=255)
                                                                      aaCTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCaa                                                                           >  3:549075/1‑149 (MQ=255)
                                                                       aCTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAGAGTAGAACTGACGCTGCaaa                                                                          <  1:186356/149‑1 (MQ=255)
                                                                           gcggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTgg                                                                      <  1:1044838/149‑1 (MQ=255)
                                                                            cggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTggg                                                                     <  3:1638983/149‑1 (MQ=255)
                                                                             ggcAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTgggg                                                                    >  4:1067500/1‑149 (MQ=255)
                                                                                aaCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCCTTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGaaa                                                                 >  4:28439/1‑149 (MQ=255)
                                                                                 aCCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAAc                                                                >  1:408628/1‑149 (MQ=255)
                                                                                 aCCGTCACGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAAc                                                                <  1:26298/149‑1 (MQ=255)
                                                                                   cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGaa                                                                  <  4:748630/145‑1 (MQ=255)
                                                                                   cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGaa                                                                  >  3:748630/1‑145 (MQ=255)
                                                                                   cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGc                                                              <  1:294708/149‑1 (MQ=255)
                                                                                   cGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGc                                                              >  4:934542/1‑149 (MQ=255)
                                                                                       aTGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACTTGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                          <  1:1104126/149‑1 (MQ=255)
                                                                                         gggCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAgg                                                        >  1:1141857/1‑149 (MQ=255)
                                                                                          ggCCGTAATGCACCACCGCCCGTGCAACGGTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGc                                                       >  3:1007027/1‑149 (MQ=255)
                                                                                          ggCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGc                                                       >  1:1585287/1‑149 (MQ=255)
                                                                                             cGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGa                                                    <  4:549075/149‑1 (MQ=255)
                                                                                             cGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGa                                                    <  1:879852/149‑1 (MQ=255)
                                                                                             cGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGa                                                    >  2:1316736/1‑149 (MQ=255)
                                                                                                aaTGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGAtgt                                                 >  3:257473/1‑149 (MQ=255)
                                                                                                   gCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                              <  1:529937/149‑1 (MQ=255)
                                                                                                   gCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                              <  4:620686/149‑1 (MQ=255)
                                                                                                   gCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                              <  2:1592518/149‑1 (MQ=255)
                                                                                                   gCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtggt                                              <  1:868920/149‑1 (MQ=255)
                                                                                                     accaccGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGa                                            >  2:1613704/1‑149 (MQ=255)
                                                                                                        accGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGAttt                                         >  2:887783/1‑149 (MQ=255)
                                                                                                             ccGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGc                                                                                                                                                    >  3:1560065/1‑37 (MQ=255)
                                                                                                             ccGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGc                                                                                                                                                    <  4:1560065/37‑1 (MQ=255)
                                                                                                              cGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTa                                   <  2:358762/149‑1 (MQ=255)
                                                                                                              cGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTa                                   <  4:1642185/149‑1 (MQ=255)
                                                                                                               gTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAt                                  <  4:252954/149‑1 (MQ=255)
                                                                                                                tGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAtt                                 <  2:861469/149‑1 (MQ=255)
                                                                                                                tGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAtt                                 <  4:385653/149‑1 (MQ=255)
                                                                                                                  cAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTgg                               <  2:774707/149‑1 (MQ=255)
                                                                                                                   aaCGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGt                              >  1:88389/1‑149 (MQ=255)
                                                                                                                     cGCTATCTTTTTCAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTAt                            <  1:985574/149‑1 (MQ=255)
                                                                                                                     cGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATg                                                                                                                          <  3:730411/55‑1 (MQ=255)
                                                                                                                     cGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTAt                            >  4:629610/1‑149 (MQ=255)
                                                                                                                       cTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCa                          >  4:1143409/1‑149 (MQ=255)
                                                                                                                         aTCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAgc                        <  3:629610/149‑1 (MQ=255)
                                                                                                                          tCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAgcg                       >  1:623245/1‑149 (MQ=255)
                                                                                                                            ttttACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGcc                     >  2:11035/1‑149 (MQ=255)
                                                                                                                                 cAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGa                <  1:385481/149‑1 (MQ=255)
                                                                                                                                   gcgcCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGc              >  1:1191192/1‑149 (MQ=255)
                                                                                                                                     gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCa                                                          >  3:496154/4‑104 (MQ=255)
                                                                                                                                     gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCa             >  4:1474700/4‑149 (MQ=255)
                                                                                                                                     gcacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCa             >  4:134130/4‑149 (MQ=255)
                                                                                                                                     gcacaccaGCCTGCTGGTGGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGAtgtg                                                <  3:918786/110‑1 (MQ=255)
                                                                                                                                      cacaccaGCCTGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTAtt                                 <  4:837946/126‑1 (MQ=255)
                                                                                                                                        caccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGccagt          <  4:72497/149‑5 (MQ=255)
                                                                                                                                        caccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACt          >  2:144261/1‑149 (MQ=255)
                                                                                                                                          ccaGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTgg        <  2:408628/149‑1 (MQ=255)
                                                                                                                                           cagcagGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGc       >  1:740592/1‑149 (MQ=255)
                                                                                                                                            agcagGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                               <  4:356232/108‑1 (MQ=255)
                                                                                                                                            agcagGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATgtgg                                               >  3:356232/1‑108 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GGTGCCGGAACTATTGGTCTGGAACTGGCTGCCAGCGCCACGCAGCGCAGATGTAAGGTGACAGTGATTGAACTGGCGGCAACCGTCATGGGCCGTAATGCACCACCGCCCGTGCAACGCTATCTTTTACAGCGCCACCAGCAGGCTGGTGTGCGCATTCTGCTCAATAATGCCATTGAACATGTGGTCGATGGTGAAAAAGTAGAACTGACGCTGCAAAGTGGGGAAACGCTTCAGGCTGATGTGGTGATTTACGGTATTGGTATCAGCGCCAACGAGCAACTGGCTCGCG  >  NC_000913/2672497‑2672788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: