Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,736,911 C→T 6.6% intergenic (+28/‑243) bamD → / → raiA BamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,736,9110CT 6.6% 538.2 / 20.1 167intergenic (+28/‑243)bamD/raiABamABCDE complex OM biogenesis lipoprotein/cold shock protein associated with 30S ribosomal subunit
Reads supporting (aligned to +/- strand):  ref base C (72/84);  new base T (2/9);  total (74/93)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.95e-01

Only 100 of 167 total aligned reads displayed.

CCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGA  >  NC_000913/2736768‑2737057
                                                                                                                                               |                                                                                                                                                  
cccAGGCTACGCGTGATGCGCCGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGACACCTGAATCACAAAACGGCAGCCCTTGa                                                                                                                                               <  2:1570170/149‑1 (MQ=255)
 ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                              >  3:1139451/1‑149 (MQ=255)
 ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                              >  2:40877/1‑149 (MQ=255)
 ccAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAg                                                                                                                                              >  1:1415830/1‑149 (MQ=255)
     gCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGc                                                                                                                                          >  2:2239/1‑149 (MQ=255)
      cTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGcc                                                                                                                                         <  1:1590742/149‑1 (MQ=255)
         cgcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtt                                                                                                                                      <  2:873951/149‑1 (MQ=255)
          gcgTGATGCGCTGCCGTTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttt                                                                                                                                     <  3:1437773/149‑1 (MQ=255)
          gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttt                                                                                                                                     <  1:1052059/149‑1 (MQ=255)
          gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttt                                                                                                                                     <  1:229006/149‑1 (MQ=255)
          gcgTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttt                                                                                                                                     >  3:1551176/1‑149 (MQ=255)
            gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                   <  3:268029/149‑1 (MQ=255)
            gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                   >  3:99927/1‑149 (MQ=255)
            gTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGttttt                                                                                                                                   <  3:797349/149‑1 (MQ=255)
             tGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                  <  3:391348/149‑1 (MQ=255)
               aTGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGttttgtgt                                                                                                                                <  1:935055/149‑5 (MQ=255)
                     tGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGt                                                                                                                          >  1:1430931/1‑149 (MQ=255)
                      gCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTc                                                                                                                         <  4:386335/149‑1 (MQ=255)
                       ccGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCa                                                                                                                        <  3:882148/149‑1 (MQ=255)
                          cTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGtt                                                                                                                     <  2:838132/149‑1 (MQ=255)
                          cTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGtt                                                                                                                     <  4:1470245/149‑1 (MQ=255)
                             aTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtttt                                                                                                                                    <  3:676546/131‑1 (MQ=255)
                             aTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtttt                                                                                                                                    <  4:1402465/131‑1 (MQ=255)
                             aTGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCTCAAGGGCTGCCGtttt                                                                                                                                    >  3:1402465/1‑131 (MQ=255)
                               ggAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaa                                                                                                                >  2:289079/1‑149 (MQ=255)
                                gAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGaaa                                                                                                               >  1:378958/1‑149 (MQ=255)
                                 aaaaTGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAAc                                                                                                              >  3:1296671/1‑149 (MQ=255)
                                   aaTGCATCCCGTCAGATGCAGATGCATGCGCACGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACATCAGCAATAGATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTg                                                                                                            >  1:1116854/1‑149 (MQ=255)
                                   aaTGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTg                                                                                                            <  3:1252910/149‑1 (MQ=255)
                                     tGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGaa                                                                                                          <  1:728381/149‑1 (MQ=255)
                                      gCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTc                                                                                                                             <  1:1066314/129‑1 (MQ=255)
                                      gCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTc                                                                                                                             >  2:1066314/1‑129 (MQ=255)
                                        aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                       >  4:835523/1‑149 (MQ=255)
                                        aTACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCg                                                                                                       >  1:871492/1‑149 (MQ=255)
                                         tACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGATACTGAAGCGAt                                                                                                     <  3:1459031/149‑1 (MQ=255)
                                          aCCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGTCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAt                                                                                                     <  4:1237192/149‑1 (MQ=255)
                                          aCCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGAAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAt                                                                                                     <  3:958115/149‑1 (MQ=255)
                                           ccGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAtt                                                                                                    <  3:583682/149‑1 (MQ=255)
                                           ccGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAtt                                                                                                    <  2:420400/149‑1 (MQ=255)
                                            cGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGAttt                                                                                                   >  3:1314935/1‑149 (MQ=255)
                                             gTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTa                                                                                                  <  3:291869/149‑1 (MQ=255)
                                             gTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTa                                                                                                  >  4:1432669/1‑149 (MQ=255)
                                              tCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAg                                                                                                 >  2:1122723/1‑149 (MQ=255)
                                                agatgcagatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTc                                                                                               >  2:1652085/1‑149 (MQ=255)
                                                      agatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATc                                                                                         >  1:1027647/1‑149 (MQ=255)
                                                       gatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                        >  3:952171/1‑149 (MQ=255)
                                                       gatgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCg                                                                                        >  2:1301293/1‑149 (MQ=255)
                                                        atgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGa                                                                                       >  4:1079013/1‑149 (MQ=255)
                                                         tgAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGACTAAATCGCttcagtt                                                                                      <  3:587954/149‑7 (MQ=255)
                                                           aaTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCGAACAGCAGCCATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGTTTTAGTCGCTATCGAtct                                                                                    <  4:128926/149‑1 (MQ=255)
                                                           aaTGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGAtct                                                                                    <  4:1529687/149‑1 (MQ=255)
                                                             tGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtca                                                                                  <  1:1193503/149‑1 (MQ=255)
                                                                gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGTAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                  >  3:1335269/1‑98 (MQ=255)
                                                                gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttttt                                                                                                                                  <  4:1335269/98‑1 (MQ=255)
                                                                gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                               >  2:750227/1‑149 (MQ=255)
                                                                gcAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCtcatca                                                                               >  1:321979/1‑149 (MQ=255)
                                                                  aaGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCaaa                                                                             >  2:1012639/1‑149 (MQ=255)
                                                                      tGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATg                                                                         >  1:544799/1‑149 (MQ=255)
                                                                      tGAAAAAGTAGCGAAAATCATCGCCACAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCt                                                                                                                            <  2:117930/98‑1 (MQ=255)
                                                                        aaaaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGc                                                                       >  2:1280344/1‑149 (MQ=255)
                                                                          aaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttt                                                                                                                                    <  1:532969/86‑1 (MQ=255)
                                                                          aaaGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGtttt                                                                                                                                    >  2:532969/1‑86 (MQ=255)
                                                                              tAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCtt                                                                 <  1:1177916/149‑1 (MQ=255)
                                                                               aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAGCAGAAACCTGAAACGCAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCttt                                                                <  4:1139451/149‑1 (MQ=255)
                                                                               aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCttt                                                                <  1:1586688/149‑1 (MQ=255)
                                                                               aGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCttt                                                                >  3:1019092/1‑149 (MQ=255)
                                                                                 cGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTga                                                              <  1:1326653/149‑1 (MQ=255)
                                                                                 cGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTga                                                              <  3:1432669/149‑1 (MQ=255)
                                                                                    aaaTCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGat                                                           >  3:372303/1‑149 (MQ=255)
                                                                                         atcGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTcc                                                      >  3:543117/1‑149 (MQ=255)
                                                                                          tcGCCGCAAACAGCAGCAATACATAACAGAAACCTTAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCt                                                     <  1:1457729/149‑1 (MQ=255)
                                                                                           cgccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                    <  1:624278/149‑1 (MQ=255)
                                                                                           cgccgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                    <  3:1079013/149‑1 (MQ=255)
                                                                                              cgcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAg                                                 >  2:618458/1‑149 (MQ=255)
                                                                                               gcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAGCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                >  2:1589472/1‑149 (MQ=255)
                                                                                               gcAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGt                                                >  4:366752/1‑149 (MQ=255)
                                                                                                   aCAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTaaaa                                            >  4:437850/1‑149 (MQ=255)
                                                                                                     agcagcAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCaa                                                  <  2:1137740/141‑1 (MQ=255)
                                                                                                         gcaATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAGGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAt                                      >  3:1635439/1‑149 (MQ=255)
                                                                                                          caATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGa                                                                                                           <  1:1015615/79‑1 (MQ=255)
                                                                                                          caATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGa                                                                                                           >  2:1015615/1‑79 (MQ=255)
                                                                                                          caATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                     >  3:211858/1‑149 (MQ=255)
                                                                                                          caATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACAtc                                     <  4:543117/149‑1 (MQ=255)
                                                                                                                  aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                    <  3:301973/126‑1 (MQ=255)
                                                                                                                  aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTc                                                    >  4:301973/1‑126 (MQ=255)
                                                                                                                  aaCAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTg                             >  3:715968/1‑149 (MQ=255)
                                                                                                                     aGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTGTTTTTATTCTGTCAGGTGTGAAACTGAAGCGATTTAGTCGCAATCGATCTCATCAAAAATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACTTCTCTTCCtccca                          >  4:1606302/1‑145 (MQ=255)
                                                                                                                           cTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtctc                    <  3:366752/149‑1 (MQ=255)
                                                                                                                           cTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTtctc                    <  1:1199756/149‑1 (MQ=255)
                                                                                                                            tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                   >  4:1129383/1‑149 (MQ=255)
                                                                                                                            tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                   >  4:699336/1‑149 (MQ=255)
                                                                                                                            tGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTca                   <  3:437850/149‑1 (MQ=255)
                                                                                                                             gAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTcac                  >  2:1379076/1‑149 (MQ=255)
                                                                                                                                  acaAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaa             >  1:300873/1‑149 (MQ=255)
                                                                                                                                   caAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa            >  3:32794/1‑149 (MQ=255)
                                                                                                                                   caAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa            <  1:413357/149‑1 (MQ=255)
                                                                                                                                    aaaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAatat                                                                          <  4:601163/86‑1 (MQ=255)
                                                                                                                                    aaaaCGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACaaaaaa            >  1:872718/1‑148 (MQ=255)
                                                                                                                                        cGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTc       <  2:871492/149‑1 (MQ=255)
                                                                                                                                             gCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGa  <  1:1652085/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CCCAGGCTACGCGTGATGCGCTGCCGCTGATGGAAAATGCATACCGTCAGATGCAGATGAATGCGCAAGCTGAAAAAGTAGCGAAAATCATCGCCGCAAACAGCAGCAATACATAACAGAAACCTGAAACACAAAACGGCAGCCCTTGAGCTGCCGTTTTTTTATTCTGTCAGTTGTGAAACTGAAGCGATTTAGTCGCTATCGATCTCATCAAATATGGCTCGCTTTGAGATATTCCTCAAGTAAAAAAACATCTCTTCCTGCGATTTCTCACAAAAAAGATTCGTTGA  >  NC_000913/2736768‑2737057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: