Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,873,915 A→G 5.9% H26H (CAT→CAC) ‡ cysC ← adenosine 5'‑phosphosulfate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,873,9150AG 5.9% 558.7 / 15.6 168H26H (CAT→CAC) ‡cysCadenosine 5'‑phosphosulfate kinase
Reads supporting (aligned to +/- strand):  ref base A (81/77);  new base G (5/5);  total (86/82)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 168 total aligned reads displayed.

AGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCA  >  NC_000913/2873769‑2874062
                                                                                                                                                  |                                                                                                                                                   
        gCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtggt                                                                                                                                           <  1:1060377/149‑1 (MQ=255)
          aTAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGgtgt                                                                                                                                         >  1:1093832/1‑149 (MQ=255)
          aTAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGgtgt                                                                                                                                         <  2:682454/149‑1 (MQ=255)
           tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGGGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                        <  3:660916/149‑1 (MQ=255)
           tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        <  3:198337/149‑1 (MQ=255)
           tAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        <  4:538635/149‑1 (MQ=255)
           tAATCCGTGGCGAACATTGTCGCCATCCAGAAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        <  1:855402/149‑1 (MQ=255)
             aTCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGc                                                                                                                                      <  3:919938/149‑1 (MQ=255)
                      gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCTCAGCCCTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             >  4:1467223/1‑149 (MQ=255)
                      gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                        <  3:841833/138‑1 (MQ=255)
                      gAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTa                                                                                                                                        >  4:841833/1‑138 (MQ=255)
                       aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                            >  4:1566566/1‑149 (MQ=255)
                       aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                            <  4:498072/149‑1 (MQ=255)
                       aaCATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgtt                                                                                                                            <  4:1371848/149‑1 (MQ=255)
                         cATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGAACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGc                                                                                                                          >  1:1221054/1‑149 (MQ=255)
                          aTTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGca                                                                                                                         <  3:1561514/149‑1 (MQ=255)
                           ttGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGcac                                                                                                                        >  4:747020/1‑149 (MQ=255)
                             gTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGttgttg                                                                                                                           <  2:1308293/144‑1 (MQ=255)
                             gTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGttgttg                                                                                                                           >  1:1308293/1‑144 (MQ=255)
                                   aTCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCg                                                                                                                >  1:947873/1‑149 (MQ=255)
                                      cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgt                                                                                                                                <  3:492732/130‑1 (MQ=255)
                                      cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  1:1055252/149‑1 (MQ=255)
                                      cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  1:200620/149‑1 (MQ=255)
                                      cagcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  2:1434012/149‑1 (MQ=255)
                                       agcagATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATg                                                                                                            >  3:625504/1‑149 (MQ=255)
                                              aCGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGc                                                                                                     >  4:480628/1‑149 (MQ=255)
                                                  aCTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACATCACTACAGCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAga                                                                                                 >  1:169290/1‑149 (MQ=255)
                                                    tGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacg                                                                                               >  4:898715/1‑149 (MQ=255)
                                                     gACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAgacga                                                                                              <  1:716392/149‑1 (MQ=255)
                                                         ccGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGAACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtt                                                                                          <  2:1221054/149‑1 (MQ=255)
                                                           gAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCgtg                                                                                                                                             >  3:839107/1‑96 (MQ=255)
                                                           gAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGtttt                                                                                        >  1:1371589/1‑149 (MQ=255)
                                                            aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGTCAGACGACGTTTTc                                                                                       <  1:911715/149‑1 (MQ=255)
                                                            aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       <  3:747020/149‑1 (MQ=255)
                                                            aGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTc                                                                                       <  4:1084355/149‑1 (MQ=255)
                                                              tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                     >  2:1265484/1‑149 (MQ=255)
                                                              tttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGt                                                                                     >  1:609679/1‑149 (MQ=255)
                                                               ttATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTc                                                                                    <  3:480628/149‑1 (MQ=255)
                                                                tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCa                                                                                   >  3:427658/1‑149 (MQ=255)
                                                                tATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCa                                                                                   <  2:549646/149‑1 (MQ=255)
                                                                 aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                                  <  2:300802/149‑1 (MQ=255)
                                                                 aTGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCAt                                                                                  <  2:1167256/149‑1 (MQ=255)
                                                                    tAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCa                                                                               >  4:308597/1‑149 (MQ=255)
                                                                      aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGcaca                                                                                                                       <  2:174637/107‑1 (MQ=255)
                                                                      aCGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                             >  4:1441719/1‑149 (MQ=255)
                                                                        gCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgcgc                                                                           >  3:180739/1‑149 (MQ=255)
                                                                         cctcctCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGcc                                                                          >  3:1253152/1‑149 (MQ=255)
                                                                          ctcctgCAGCGCCCCGGCGACCGTTGATTTACCCGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCa                                                                         >  1:489860/1‑149 (MQ=255)
                                                                          ctcctcCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCa                                                                         >  3:1154787/1‑149 (MQ=255)
                                                                           tcctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCat                                                                        >  3:1018706/1‑149 (MQ=255)
                                                                             ctccAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCgttgt                                                                                                                             <  2:1502718/94‑1 (MQ=255)
                                                                                 aGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttattta                                                                  <  2:982694/149‑1 (MQ=255)
                                                                                  gcgcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCAttatttat                                                                 <  1:1251164/149‑1 (MQ=255)
                                                                                    gcCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATcc                                                               <  4:427658/149‑1 (MQ=255)
                                                                                     ccccGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             >  3:1362975/1‑102 (MQ=255)
                                                                                       ccGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        >  1:1607967/1‑73 (MQ=38)
                                                                                       ccGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGGTCATCGTGGTGTa                                                                                                                                        <  2:1607967/73‑1 (MQ=37)
                                                                                            gACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCaa                                                       >  1:1539349/1‑149 (MQ=255)
                                                                                               cGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                    <  2:1427694/149‑1 (MQ=255)
                                                                                               cGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATc                                                    <  1:539719/149‑1 (MQ=255)
                                                                                                gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                                   >  1:1669259/1‑149 (MQ=255)
                                                                                                gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                                   >  2:501382/1‑149 (MQ=255)
                                                                                                gTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcg                                                   >  2:347292/1‑149 (MQ=255)
                                                                                                 ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCa                                                           >  2:782338/1‑140 (MQ=255)
                                                                                                 ttGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCa                                                           <  1:782338/140‑1 (MQ=255)
                                                                                                   gATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgc                                                <  2:1589883/149‑1 (MQ=255)
                                                                                                    aTTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCat                                                                        <  4:64862/124‑1 (MQ=255)
                                                                                                    aTTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgcg                                               >  3:1390798/1‑149 (MQ=255)
                                                                                                     tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGAt                                                                                                             <  4:840483/86‑1 (MQ=255)
                                                                                                     tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGcc                                                                          >  1:868857/1‑121 (MQ=255)
                                                                                                     tttACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgc                                                <  4:696046/147‑1 (MQ=255)
                                                                                                           ggACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCa                                        >  4:134845/1‑149 (MQ=255)
                                                                                                               ccGGAGAGGCCGGTAAACCACAGCACTACACTACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGc                                    <  2:548168/149‑1 (MQ=255)
                                                                                                                      ggCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGt                             <  2:365826/149‑1 (MQ=255)
                                                                                                                       gCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTg                            >  3:1083137/1‑149 (MQ=255)
                                                                                                                        ccGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTgg                           <  3:1566566/149‑1 (MQ=255)
                                                                                                                         cGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGc                          <  2:1093832/149‑1 (MQ=255)
                                                                                                                          ggTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGcg                         >  2:772180/1‑149 (MQ=255)
                                                                                                                            tAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                             >  1:827250/1‑95 (MQ=255)
                                                                                                                            tAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAgc                                                                             <  2:827250/95‑1 (MQ=255)
                                                                                                                                ccACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAc                   >  4:98021/1‑149 (MQ=255)
                                                                                                                                ccACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAc                   >  2:1077214/1‑149 (MQ=255)
                                                                                                                                 cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAcc                  >  2:611191/1‑149 (MQ=255)
                                                                                                                                 cacaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAAcc                  >  4:1340536/1‑149 (MQ=255)
                                                                                                                                  acaGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCa                 >  1:599400/1‑149 (MQ=255)
                                                                                                                                   caGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgc                                                >  2:180663/1‑117 (MQ=255)
                                                                                                                                   caGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATcgcgc                                                <  1:180663/117‑1 (MQ=255)
                                                                                                                                    aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAATTCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga               <  2:88509/149‑1 (MQ=255)
                                                                                                                                    aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga               <  1:337321/149‑1 (MQ=255)
                                                                                                                                    aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga               <  2:842979/149‑1 (MQ=255)
                                                                                                                                    aGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCaga               <  3:1677853/149‑1 (MQ=255)
                                                                                                                                       aCTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTTCGGAAAGTGGCGACGAACCAGAGCa            >  4:793774/1‑149 (MQ=255)
                                                                                                                                         tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt          >  3:1129636/1‑149 (MQ=255)
                                                                                                                                         tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt          >  1:991982/1‑149 (MQ=255)
                                                                                                                                         tACACCACGGTCATCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCAtt          >  1:462697/1‑149 (MQ=255)
                                                                                                                                            accacGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCaa       <  2:1669259/149‑1 (MQ=255)
                                                                                                                                               acGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTc    <  1:1192761/149‑1 (MQ=255)
                                                                                                                                                 gATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCa  <  2:866690/149‑1 (MQ=255)
                                                                                                                                                 gATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCa  <  3:1441719/149‑1 (MQ=255)
                                                                                                                                                 gATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCa  <  3:98021/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
AGATCGCTGCATAATCCGTGGCGAACATTGTCGCCATCCAGCAGATACGTACTGACGCCGAGTTTATGTAACGCCTCCTCCAGCGCCCCGGCGACCGTTGATTTACCGGACCCGGAGAGGCCGGTAAACCACAGCACTACACCACGATGACCGTGGTGTAGCTCGCGTTGTTGCACAGTGACCGGATGGCTATGCCAGACGACGTTTTCGTCATGCAGCGCCATTATTTATCCCCCAGCAAATCGCGCGCGCCCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCA  >  NC_000913/2873769‑2874062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: