Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,890,619 T→A 6.1% D427V (GAC→GTC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,890,6190TA 6.1% 518.2 / 17.7 150D427V (GAC→GTC) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (66/74);  new base A (2/7);  total (68/82)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 151 total aligned reads displayed.

CGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATT  >  NC_000913/2890473‑2890767
                                                                                                                                                  |                                                                                                                                                    
cggcgCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCa                                                                                                                                                    <  1:694524/149‑1 (MQ=255)
 ggcgCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAg                                                                                                                                                   <  1:724068/149‑1 (MQ=255)
    gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                <  2:1563392/149‑1 (MQ=255)
    gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                <  3:64631/149‑1 (MQ=255)
    gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGCGCCTGGGCCCATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTACTTCCACGCGGTCAGCGa                                                                                                                                                <  4:129812/149‑1 (MQ=255)
        tgctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGaa                                                                                                                                            <  4:612426/149‑1 (MQ=255)
          ctgcATAAAGGCGCGGAACGGCGCAATAGCGGTGCCTGGGCCAATCATAATCACCGTGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGTCTTCGCCCTCTTCTTCCACGCGGGCAGCGAGGAAGc                                                                                                                                          <  2:1137130/149‑1 (MQ=255)
          ctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAga                                                                                                                                          <  3:492464/149‑2 (MQ=255)
          ctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGc                                                                                                                                          <  3:1665301/149‑1 (MQ=255)
           tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                         <  3:745501/149‑3 (MQ=255)
           tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                         <  3:885940/149‑3 (MQ=255)
           tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCt                                                                                                                                         >  3:801864/1‑149 (MQ=255)
              aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                      >  3:151239/1‑149 (MQ=255)
               tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaagagg                                                                                                                                     <  3:225077/149‑7 (MQ=255)
               tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                     >  1:1334683/1‑149 (MQ=255)
                  aGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                     <  1:62833/146‑1 (MQ=255)
                              gcgcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACgg                                                                                                                      <  4:1390479/149‑1 (MQ=255)
                                gcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                      >  4:783777/1‑131 (MQ=255)
                                gcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                      <  3:783777/131‑1 (MQ=255)
                                 cAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcg                                                                                                                   >  4:1277518/1‑149 (MQ=255)
                                  aaTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcgc                                                                                                                  >  1:1042481/1‑149 (MQ=255)
                                   aTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGcgcg                                                                                                                 <  4:801864/149‑1 (MQ=255)
                                         gTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGAGGCCTTc                                                                                                           <  2:926108/149‑1 (MQ=255)
                                          tGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTcc                                                                                                          <  1:1687182/149‑1 (MQ=255)
                                             cTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcg                                                                                                       >  3:1343612/1‑149 (MQ=255)
                                              tGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcgt                                                                                                      <  2:1042481/149‑1 (MQ=255)
                                               gggCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcgtc                                                                                                     <  3:361081/149‑1 (MQ=255)
                                                  ccAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTa                                                                                                  >  1:1557922/1‑149 (MQ=255)
                                                   cAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATATATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTaa                                                                                                 <  2:201614/149‑1 (MQ=255)
                                                    aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTgg                                                                                                                                       <  2:517243/110‑1 (MQ=255)
                                                    aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTgg                                                                                                                                       >  1:517243/1‑110 (MQ=255)
                                                    aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                >  3:336765/1‑149 (MQ=255)
                                                    aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                >  2:690675/1‑149 (MQ=255)
                                                    aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTACCACGTCGTAAc                                                                                                >  1:1359694/1‑149 (MQ=255)
                                                      tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                         <  2:827732/106‑3 (MQ=255)
                                                      tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                         >  1:827732/1‑104 (MQ=255)
                                                       cATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGca                                                                                             >  2:1222974/1‑149 (MQ=255)
                                                         tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCGCGCGGTCAGCGAGGAAGCTGGAGGCACAACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcacc                                                                                           >  2:618118/1‑149 (MQ=255)
                                                         tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                      >  3:1623383/1‑106 (MQ=255)
                                                         tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                      <  4:1623383/106‑1 (MQ=255)
                                                            tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                        >  1:216071/1‑149 (MQ=255)
                                                                cGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCaa                                                                                    <  4:232608/149‑1 (MQ=255)
                                                                 ggggTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAAc                                                                                   <  2:1108613/149‑1 (MQ=255)
                                                                    gTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGt                                                                                <  2:421110/149‑1 (MQ=255)
                                                                     tttCTGGATTGGTTGGAAGGCGGAGGTTATGGTTTGGTTGGATAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGa                                                                              <  2:522572/149‑1 (MQ=255)
                                                                       tCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                             <  2:1335768/149‑1 (MQ=255)
                                                                          ggATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtg                                                                          >  1:235132/1‑149 (MQ=255)
                                                                             ttGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                  >  3:558734/1‑138 (MQ=255)
                                                                             ttGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                  <  4:558734/138‑1 (MQ=255)
                                                                                gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTc                                                                    >  1:1058750/1‑149 (MQ=255)
                                                                                gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTc                                                                    >  1:525394/1‑149 (MQ=255)
                                                                                   ggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGtt                                                                 >  2:848235/1‑149 (MQ=255)
                                                                                    gcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtc                                                                >  3:378977/1‑149 (MQ=255)
                                                                                     cAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtct                                                               <  2:662344/149‑1 (MQ=255)
                                                                                           gAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcac                                                                                            >  3:554273/1‑114 (MQ=255)
                                                                                           gAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcac                                                                                            <  4:554273/114‑1 (MQ=255)
                                                                                            aaGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                             <  3:519694/128‑1 (MQ=255)
                                                                                                 aTCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCt                                                   >  4:1624336/1‑149 (MQ=255)
                                                                                                  tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTg                                                  >  4:694286/1‑149 (MQ=255)
                                                                                                   cGTTATGTTCGATAAATACGCGGACTTCGCCCTTTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGc                                                 >  3:1067902/1‑149 (MQ=255)
                                                                                                     ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCga                                               >  2:420508/1‑149 (MQ=255)
                                                                                                        tGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgagga                                            <  4:985006/149‑1 (MQ=255)
                                                                                                        tGTTCGATAAATACGCGGACTTCGCCCTCTGCGTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgagga                                            <  1:1707573/149‑1 (MQ=255)
                                                                                                          ttCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                          <  2:235132/149‑1 (MQ=255)
                                                                                                             gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                       <  1:690675/149‑1 (MQ=255)
                                                                                                             gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                       >  3:968783/1‑149 (MQ=255)
                                                                                                             gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                       <  2:465161/149‑1 (MQ=255)
                                                                                                              aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                      >  1:1029757/1‑149 (MQ=255)
                                                                                                              aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                      <  4:378977/149‑1 (MQ=255)
                                                                                                              aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                      <  4:1392736/149‑1 (MQ=255)
                                                                                                              aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                      <  2:1082803/149‑1 (MQ=255)
                                                                                                                  aTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGaa                                  >  2:1107586/1‑149 (MQ=255)
                                                                                                                    aCGCGGACTTCGCCTTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                <  4:1247045/149‑1 (MQ=255)
                                                                                                                    aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                <  4:469776/149‑1 (MQ=255)
                                                                                                                    aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                <  2:1557922/149‑1 (MQ=255)
                                                                                                                    aCGCGGACATCGCCGTCTTCTTCCACGCGGTGAGCGAGGAAGCTTGAGGCACCACCGGCACGGGCGCGGCCTTCGACGTCGTAACGCACCACCCCAACGGTGACGTGTACTTCGTTCTCGTCTTCCGCCTGCGAGGAGGCGATGGAATa                                <  3:1412651/149‑1 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                  >  4:1686745/1‑97 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                  <  3:1686745/97‑1 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtgt                                                                         >  1:1228829/1‑106 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                              <  2:720618/149‑1 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                              <  2:941484/149‑1 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                              >  1:810420/1‑149 (MQ=255)
                                                                                                                      gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                              >  2:981538/1‑149 (MQ=255)
                                                                                                                          aCTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGCGGCAACACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACTACACCAACGGTGACGTGTACTTCGTTCTCGACTGCCGCCTGCGAGGAGGCGATGGAATACAGAcg                          <  2:478479/149‑1 (MQ=255)
                                                                                                                           cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                         <  2:1024404/149‑1 (MQ=255)
                                                                                                                           cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                         <  1:373283/149‑1 (MQ=255)
                                                                                                                           cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                         <  1:1222974/149‑1 (MQ=255)
                                                                                                                            ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                      <  3:1325234/135‑1 (MQ=255)
                                                                                                                            ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgcg                        >  3:1058869/1‑149 (MQ=255)
                                                                                                                              cGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                      <  1:1107586/149‑1 (MQ=255)
                                                                                                                                 ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                              <  1:971595/106‑1 (MQ=255)
                                                                                                                                 ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt                    <  1:286504/148‑1 (MQ=255)
                                                                                                                                 ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt                    >  2:286504/1‑148 (MQ=255)
                                                                                                                                    cttcttCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                              >  2:1512195/1‑135 (MQ=255)
                                                                                                                                     agcttcCTCGCTGACCGCGTGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCg               >  2:1624343/3‑149 (MQ=255)
                                                                                                                                            aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCaa                                                                                    <  2:621694/73‑1 (MQ=255)
                                                                                                                                            aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTCCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag        >  3:156230/1‑149 (MQ=255)
                                                                                                                                                ggTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa    <  3:1007772/149‑1 (MQ=255)
                                                                                                                                                ggTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa    <  3:716303/149‑1 (MQ=255)
                                                                                                                                                 gTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCggg             <  1:1529529/139‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGGCGCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATT  >  NC_000913/2890473‑2890767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: