Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,890,625 A→T 5.8% L425H (CTC→CAC)  cysJ ← sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,890,6250AT 5.8% 529.4 / 17.9 154L425H (CTC→CAC) cysJsulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand):  ref base A (71/74);  new base T (2/7);  total (73/81)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

Only 100 of 160 total aligned reads displayed.

GCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAG  >  NC_000913/2890477‑2890773
                                                                                                                                                    |                                                                                                                                                    
gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGa                                                                                                                                                      <  3:64631/149‑1 (MQ=255)
gcgTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGCGCCTGGGCCCATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTACTTCCACGCGGTCAGCGa                                                                                                                                                      <  4:129812/149‑1 (MQ=255)
    tgctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGaa                                                                                                                                                  <  4:612426/149‑1 (MQ=255)
      ctgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAga                                                                                                                                                <  3:492464/149‑2 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                               <  3:885940/149‑3 (MQ=255)
       tgcATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                               <  3:745501/149‑3 (MQ=255)
          aTAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                            <  3:187747/149‑1 (MQ=255)
           tAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                           >  1:1334683/1‑149 (MQ=255)
              aGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCTCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGca                                                                                                                                        <  2:857706/149‑1 (MQ=255)
              aGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAg                                                                                                                                           >  2:62833/1‑146 (MQ=255)
                         ggCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACg                                                                                                                             >  3:1390479/1‑149 (MQ=255)
                          gcgcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACgg                                                                                                                            <  4:1390479/149‑1 (MQ=255)
                            gcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                            >  4:783777/1‑131 (MQ=255)
                            gcAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                            <  3:783777/131‑1 (MQ=255)
                             cAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcg                                                                                                                         >  4:1277518/1‑149 (MQ=255)
                              aaTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcgc                                                                                                                        >  2:691288/1‑149 (MQ=255)
                              aaTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcgc                                                                                                                        >  1:1042481/1‑149 (MQ=255)
                              aaTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGgcgc                                                                                                                        >  1:256408/1‑149 (MQ=255)
                               aTACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGcgcg                                                                                                                       <  4:801864/149‑1 (MQ=255)
                                     gTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGAGGCCTTc                                                                                                                 <  2:926108/149‑1 (MQ=255)
                                      tGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTcc                                                                                                                <  1:1687182/149‑1 (MQ=255)
                                          tGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCAcgt                                                                                                            <  2:1042481/149‑1 (MQ=255)
                                               cAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTaa                                                                                                       <  4:1632520/149‑1 (MQ=255)
                                                aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTgg                                                                                                                                             >  1:517243/1‑110 (MQ=255)
                                                aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTgg                                                                                                                                             <  2:517243/110‑1 (MQ=255)
                                                aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                      >  3:336765/1‑149 (MQ=255)
                                                aaTCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTCCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                      >  2:800731/1‑149 (MQ=255)
                                                aaTCATAATCACCGGGGCTTCTGGATTGGCCGGCATGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTGGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGTACGGGCGCGGCCTTCCACGTCGTAAc                                                                                                      >  4:1412651/1‑149 (MQ=255)
                                                  tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                               >  1:827732/1‑104 (MQ=255)
                                                  tCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCTCGCTGACCGCGTGGAAgaa                                                                                                                                               <  2:827732/106‑3 (MQ=255)
                                                   cATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGca                                                                                                   >  2:1222974/1‑149 (MQ=255)
                                                     tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCGCGCGGTCAGCGAGGAAGCTGGAGGCACAACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcacc                                                                                                 >  2:618118/1‑149 (MQ=255)
                                                     tAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGa                                                                                                                                            <  4:1623383/106‑1 (MQ=255)
                                                      aaTCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcacca                                                                                                >  3:787028/1‑149 (MQ=255)
                                                        tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                              >  1:216071/1‑149 (MQ=255)
                                                        tCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcaccaca                                                                                              <  1:691288/149‑1 (MQ=255)
                                                            cGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCaa                                                                                          <  2:216071/149‑1 (MQ=255)
                                                             ggggTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAAc                                                                                         >  1:421110/1‑149 (MQ=255)
                                                                gTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGt                                                                                      <  2:421110/149‑1 (MQ=255)
                                                                   tCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                                   <  2:1335768/149‑1 (MQ=255)
                                                                         ttGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                        <  4:558734/138‑1 (MQ=255)
                                                                         ttGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                        >  3:558734/1‑138 (MQ=255)
                                                                            gctggcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTc                                                                          >  1:1058750/1‑149 (MQ=255)
                                                                                gcAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtc                                                                      >  3:378977/1‑149 (MQ=255)
                                                                                 cAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtct                                                                     <  2:662344/149‑1 (MQ=255)
                                                                                  aGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                                    <  3:1636958/149‑1 (MQ=255)
                                                                                       gAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGcac                                                                                                  >  3:554273/1‑114 (MQ=255)
                                                                                        aaGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                                   <  3:519694/128‑1 (MQ=255)
                                                                                        aaGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGAc                                                                                   >  4:519694/1‑128 (MQ=255)
                                                                                          gTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCg                                                            >  2:573100/1‑149 (MQ=255)
                                                                                              tCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTg                                                        >  4:694286/1‑149 (MQ=255)
                                                                                               cGTTATGTTCGATAAATACGCGGACTTCGCCCTTTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGc                                                       >  3:1067902/1‑149 (MQ=255)
                                                                                                 ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCga                                                     >  2:420508/1‑149 (MQ=255)
                                                                                                 ttATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCga                                                     >  2:1087589/1‑149 (MQ=255)
                                                                                                    tGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCgagga                                                  <  4:985006/149‑1 (MQ=255)
                                                                                                      ttCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGaggagg                                                <  2:235132/149‑1 (MQ=255)
                                                                                                         gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                             >  3:968783/1‑149 (MQ=255)
                                                                                                         gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                             <  2:465161/149‑1 (MQ=255)
                                                                                                         gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGa                                             <  1:690675/149‑1 (MQ=255)
                                                                                                          aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                            >  1:1029757/1‑149 (MQ=255)
                                                                                                          aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                            <  2:1029757/149‑1 (MQ=255)
                                                                                                          aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                            <  4:378977/149‑1 (MQ=255)
                                                                                                              aTACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGaa                                        >  2:1107586/1‑149 (MQ=255)
                                                                                                                aCGCGGACTTCGCCTTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                      <  4:1247045/149‑1 (MQ=255)
                                                                                                                aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                      >  3:1513676/1‑149 (MQ=255)
                                                                                                                aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATa                                      <  2:1557922/149‑1 (MQ=255)
                                                                                                                aCGCGGACATCGCCGTCTTCTTCCACGCGGTGAGCGAGGAAGCTTGAGGCACCACCGGCACGGGCGCGGCCTTCGACGTCGTAACGCACCACCCCAACGGTGACGTGTACTTCGTTCTCGTCTTCCGCCTGCGAGGAGGCGATGGAATa                                      <  3:1412651/149‑1 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                        >  4:1686745/1‑97 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACg                                                                                        <  3:1686745/97‑1 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACgtgt                                                                               <  2:1228829/106‑1 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                                    >  1:810420/1‑149 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                                    <  1:179594/149‑1 (MQ=255)
                                                                                                                  gcgGACTTCGCCCTCTACTTCCACGCTGTCAGCGAGGAAGCTGGAGGCACCACCGACACGGGCGCGTCCTTCCACGACGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCTAGGAGGCGATGGAATACa                                    <  2:112607/149‑1 (MQ=255)
                                                                                                                    ggACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGa                                  >  2:1593457/1‑149 (MQ=255)
                                                                                                                      aCTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGCGGCAACACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACTACACCAACGGTGACGTGTACTTCGTTCTCGACTGCCGCCTGCGAGGAGGCGATGGAATACAGAcg                                <  2:478479/149‑1 (MQ=255)
                                                                                                                       cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                               <  1:373283/149‑1 (MQ=255)
                                                                                                                       cTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGAcgc                               <  1:1222974/149‑1 (MQ=255)
                                                                                                                        ttCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGAt                                            <  3:1325234/135‑1 (MQ=255)
                                                                                                                          cGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggc                            <  1:1107586/149‑1 (MQ=255)
                                                                                                                           gCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg                           >  1:1291913/1‑149 (MQ=255)
                                                                                                                           gCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGAACAACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACgcggcg                           >  3:725221/1‑149 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                                    >  2:971595/1‑106 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTtctc                                                                    <  1:971595/106‑1 (MQ=255)
                                                                                                                             ccTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGt                          >  2:286504/1‑148 (MQ=255)
                                                                                                                                cttcttCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                                    <  1:1512195/135‑1 (MQ=255)
                                                                                                                                cttcttCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACa                                    >  2:1512195/1‑135 (MQ=255)
                                                                                                                                 agcttcCTCGCTGACCGCGTGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCg                     >  2:1624343/3‑149 (MQ=255)
                                                                                                                                       cACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgca               >  3:359124/1‑149 (MQ=255)
                                                                                                                                        aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCaa                                                                                          >  1:621694/1‑73 (MQ=255)
                                                                                                                                        aCGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTCCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgcag              >  3:156230/1‑149 (MQ=255)
                                                                                                                                            ggTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGa          <  3:1007772/149‑1 (MQ=255)
                                                                                                                                             gTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCggg                   <  1:1529529/139‑1 (MQ=255)
                                                                                                                                              tCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGatt        >  3:1039186/1‑149 (MQ=255)
                                                                                                                                                aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaa      >  1:414021/1‑149 (MQ=255)
                                                                                                                                                aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaa      >  4:387115/1‑149 (MQ=255)
                                                                                                                                                aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGGGTATTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGTGCGCAGCAGattaa      >  4:520215/1‑149 (MQ=255)
                                                                                                                                                 gCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaat     >  3:1183096/1‑149 (MQ=255)
                                                                                                                                                  cGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGattaatt    <  1:1593457/149‑1 (MQ=255)
                                                                                                                                                    aGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAg  <  4:336765/149‑1 (MQ=255)
                                                                                                                                                    aGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAg  >  1:1681339/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCGTTGCTGCATAAAGGCGCGGAACGGCGCAATACCGGTGCCTGGGCCAATCATAATCACCGGGGTTTCTGGATTGGCTGGCAGGCGGAAGTTATCGTTATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAG  >  NC_000913/2890477‑2890773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: