Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,118 C→A 5.3% P343T (CCG→ACG)  dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,1180CA 5.3% 465.9 / 10.8 132P343T (CCG→ACG) dnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base C (64/61);  new base A (5/2);  total (69/63)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 134 total aligned reads displayed.

TGGTACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGC  >  NC_000913/492972‑493265
                                                                                                                                                  |                                                                                                                                                   
tGGTACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCg                                                                                                                                                   >  2:1507175/1‑149 (MQ=255)
   tACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAc                                                                                                                                                >  2:109891/1‑149 (MQ=255)
    aCAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAcc                                                                                                                                               >  2:1353854/1‑149 (MQ=255)
    aCAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAcc                                                                                                                                               >  2:1202464/1‑149 (MQ=255)
        cTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCg                                                                                                                                           >  4:8032/1‑149 (MQ=255)
         tttCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGc                                                                                                                                          >  2:769968/1‑149 (MQ=255)
           tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                        <  2:719536/149‑1 (MQ=255)
           tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                        <  1:466626/149‑1 (MQ=255)
             gCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATgg                                                                                                                                      <  3:424018/149‑1 (MQ=255)
               ctgctgCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGc                                                                                                                                    <  3:1506624/149‑1 (MQ=255)
               ctgctgCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGc                                                                                                                                    <  2:1594801/149‑1 (MQ=255)
                  ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTATCTTATGTAATTGTCCGTCGCATggaccat                                                                                                                                 <  2:1321284/149‑6 (MQ=255)
                  ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGACGGTCTGGCGCAtacggtaa                                                                                                                                 <  3:118455/149‑8 (MQ=255)
                  ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATAACGCCGACGGATATTCAGCTTTACCATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGtt                                                                                                                                 <  1:1585914/149‑1 (MQ=255)
                   tgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTg                                                                                                                                >  1:530092/1‑149 (MQ=255)
                    gcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTga                                                                                                                               <  2:722721/149‑1 (MQ=255)
                    gcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTga                                                                                                                               <  4:1096893/149‑1 (MQ=255)
                       cTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGAt                                                                                                                            <  2:875494/149‑1 (MQ=255)
                         tGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGa                                                                                                                          <  1:80165/149‑1 (MQ=255)
                          ggCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGAc                                                                                                                         <  1:1568502/149‑1 (MQ=255)
                            cAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACgc                                                                                                                       <  4:729885/149‑1 (MQ=255)
                                    tGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgc                                                                                                               <  2:530092/149‑1 (MQ=255)
                                     ggCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcg                                                                                                              >  2:1439908/1‑149 (MQ=255)
                                       ccgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcg                                                                                                            >  4:731416/1‑149 (MQ=255)
                                        cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                           <  2:371375/149‑1 (MQ=255)
                                          ccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTg                                                                                                         >  2:929228/1‑149 (MQ=255)
                                          ccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTg                                                                                                         <  1:85805/149‑1 (MQ=255)
                                               gAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCAtt                                                                                                    <  4:425115/149‑1 (MQ=255)
                                                   tGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCAt                                                                                                <  1:675003/149‑1 (MQ=255)
                                                    gCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATc                                                                                               >  3:659015/1‑149 (MQ=255)
                                                         tGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGt                                                                                          <  4:406333/149‑1 (MQ=255)
                                                            gTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATg                                                                                       >  2:270829/1‑149 (MQ=255)
                                                            gTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATg                                                                                       >  4:1125744/1‑149 (MQ=255)
                                                             tGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGc                                                                                      >  3:770562/1‑149 (MQ=255)
                                                              gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                     >  1:1696092/1‑149 (MQ=255)
                                                              gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                     >  3:9452/1‑149 (MQ=255)
                                                              gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                     <  1:769968/149‑1 (MQ=255)
                                                               aaCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCg                                                                                    <  1:1279072/149‑1 (MQ=255)
                                                                aCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGc                                                                                   <  3:1139968/149‑1 (MQ=255)
                                                                    gcgcgcACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATgg                                                                                                                                      >  4:558015/1‑94 (MQ=255)
                                                                    gcgcgcACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGAATgg                                                                                                                                      <  3:558015/94‑1 (MQ=255)
                                                                         cACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGc                                                                          <  2:816649/149‑1 (MQ=255)
                                                                         cACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGc                                                                          >  2:7999/1‑149 (MQ=255)
                                                                             aTACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGa                                                                      >  2:1553666/1‑149 (MQ=255)
                                                                              tACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGaa                                                                     <  4:1401172/149‑1 (MQ=255)
                                                                                     gACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCAc                                                              <  4:169087/149‑1 (MQ=255)
                                                                                        ggATATTCAGCTTTATTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCac                                                                       <  4:1090310/137‑2 (MQ=255)
                                                                                        ggATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAg                                                                       >  3:1090310/1‑137 (MQ=255)
                                                                                         gATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACa                                                          >  1:152381/1‑149 (MQ=255)
                                                                                         gATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACa                                                          >  2:1098195/1‑149 (MQ=255)
                                                                                          atatTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAg                                                         <  1:1367167/149‑1 (MQ=255)
                                                                                           tatTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGt                                                        >  1:1135358/1‑149 (MQ=255)
                                                                                              tCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     >  1:1269846/1‑149 (MQ=255)
                                                                                              tCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     <  1:989401/149‑1 (MQ=255)
                                                                                                aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                   <  1:109891/149‑1 (MQ=255)
                                                                                                aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                   <  4:659015/149‑1 (MQ=255)
                                                                                                   tttACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCa                                                >  2:531494/1‑149 (MQ=255)
                                                                                                     tACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAcc                                              >  3:149429/1‑149 (MQ=255)
                                                                                                      aCTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTgag                                                                                                                              >  3:394084/1‑68 (MQ=255)
                                                                                                      aCTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTgag                                                                                                                              <  4:394084/68‑1 (MQ=255)
                                                                                                        tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGa                                                                            <  3:854407/116‑1 (MQ=255)
                                                                                                        tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                           >  4:1208409/1‑149 (MQ=255)
                                                                                                         aTCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAccc                                             <  3:334585/146‑1 (MQ=255)
                                                                                                          tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  1:325227/1‑149 (MQ=255)
                                                                                                          tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  4:1352420/1‑149 (MQ=255)
                                                                                                          tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  2:367360/1‑149 (MQ=255)
                                                                                                             gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                      >  1:7586/1‑133 (MQ=255)
                                                                                                             gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCgcgc                                      <  1:270829/149‑1 (MQ=255)
                                                                                                             gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCgcgc                                      >  1:344420/1‑149 (MQ=255)
                                                                                                               cGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCa                                    >  2:1434111/1‑149 (MQ=255)
                                                                                                                gCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCaa                                   <  1:929228/149‑1 (MQ=255)
                                                                                                                  tGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACg                                 <  3:1352420/149‑1 (MQ=255)
                                                                                                                   gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCt                                                                              <  2:361222/103‑1 (MQ=255)
                                                                                                                   gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                <  4:848793/149‑1 (MQ=255)
                                                                                                                    ttGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGc                               >  1:1708932/1‑149 (MQ=255)
                                                                                                                    ttGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGc                               >  3:1173612/1‑149 (MQ=255)
                                                                                                                     tGATTGGTCGCAAAGCATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTTCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                              <  2:1470857/149‑1 (MQ=255)
                                                                                                                     tGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                              <  1:1145747/149‑1 (MQ=255)
                                                                                                                      gATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAg                             >  1:916319/1‑149 (MQ=255)
                                                                                                                        ttGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTa                           >  1:757392/1‑149 (MQ=255)
                                                                                                                         tGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTaa                          >  1:699617/1‑149 (MQ=255)
                                                                                                                            tCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGa                       <  1:1439908/149‑1 (MQ=255)
                                                                                                                                aaaGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGGTGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGcc                   >  1:1668448/1‑149 (MQ=255)
                                                                                                                                aaaGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGcc                   >  1:1259240/1‑149 (MQ=255)
                                                                                                                                aaaGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGcc                   >  4:1593010/1‑149 (MQ=255)
                                                                                                                                    aaTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     <  3:554464/111‑1 (MQ=255)
                                                                                                                                    aaTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     >  4:554464/1‑111 (MQ=255)
                                                                                                                                     aTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAAccc              <  2:1135358/149‑1 (MQ=255)
                                                                                                                                      ttACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCa             >  1:1383330/1‑149 (MQ=255)
                                                                                                                                       tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg            <  1:860937/149‑1 (MQ=255)
                                                                                                                                         gcccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGt          >  4:534682/5‑149 (MQ=255)
                                                                                                                                         acGTATGCTCCGGACCGGCGCATGGGCGTTGGGATGACGGTGCTGCCCGGGATGGCATTCCACCCGCGTAAGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTACTTTGCTCACGTAGCGCCAACGGTAGTAATGACGCCAACCCAGGt          <  2:413243/148‑1 (MQ=255)
                                                                                                                                           ccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgc        >  3:223018/3‑149 (MQ=255)
                                                                                                                                           ccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgc        >  4:1464832/3‑149 (MQ=255)
                                                                                                                                            tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc       <  2:699617/149‑1 (MQ=255)
                                                                                                                                            tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc       >  2:670999/1‑149 (MQ=255)
                                                                                                                                            tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGCTgcc       >  3:695643/1‑149 (MQ=255)
                                                                                                                                            caTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc       >  1:614865/2‑149 (MQ=255)
                                                                                                                                             aTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgccg      >  3:931464/1‑149 (MQ=255)
                                                                                                                                               gcgcCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgccgcc    <  1:446004/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TGGTACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGC  >  NC_000913/492972‑493265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: