Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,122 A→T 5.1% D344V (GAC→GTC)  dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,1220AT 5.1% 470.5 / 10.2 133D344V (GAC→GTC) dnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base A (64/62);  new base T (5/2);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

Only 100 of 133 total aligned reads displayed.

TACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGC  >  NC_000913/492975‑493265
                                                                                                                                                   |                                                                                                                                               
tACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAc                                                                                                                                                >  2:109891/1‑149 (MQ=255)
 aCAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAcc                                                                                                                                               >  2:675003/1‑149 (MQ=255)
 aCAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAcc                                                                                                                                               >  4:762782/1‑149 (MQ=255)
 aCAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGAcc                                                                                                                                               >  2:1353854/1‑149 (MQ=255)
   aaCTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGt                                                                                                                                             >  1:1603322/1‑149 (MQ=255)
     cTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCg                                                                                                                                           >  4:8032/1‑149 (MQ=255)
      tttCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGc                                                                                                                                          >  2:769968/1‑149 (MQ=255)
        tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                        <  2:719536/149‑1 (MQ=255)
        tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                        <  2:109691/149‑1 (MQ=255)
        tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                        <  1:466626/149‑1 (MQ=255)
          gCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATgg                                                                                                                                      <  3:424018/149‑1 (MQ=255)
            ctgctgCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGc                                                                                                                                    <  2:1594801/149‑1 (MQ=255)
              gctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGt                                                                                                                                  >  4:1139968/1‑149 (MQ=255)
               ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTATCTTATGTAATTGTCCGTCGCATggaccat                                                                                                                                 <  2:1321284/149‑6 (MQ=255)
               ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGtt                                                                                                                                 <  2:1429315/149‑1 (MQ=255)
               ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATAACGCCGACGGATATTCAGCTTTACCATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGtt                                                                                                                                 <  1:1585914/149‑1 (MQ=255)
                tgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTg                                                                                                                                >  1:530092/1‑149 (MQ=255)
                 gcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTga                                                                                                                               <  4:1096893/149‑1 (MQ=255)
                    cTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGAt                                                                                                                            <  2:875494/149‑1 (MQ=255)
                       ggCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGAc                                                                                                                         <  1:1568502/149‑1 (MQ=255)
                         cAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACgc                                                                                                                       <  4:729885/149‑1 (MQ=255)
                                aTGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcg                                                                                                                >  2:1145747/1‑149 (MQ=255)
                                 tGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgc                                                                                                               <  2:530092/149‑1 (MQ=255)
                                  ggCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcg                                                                                                              >  2:1439908/1‑149 (MQ=255)
                                     cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                           <  3:968945/149‑1 (MQ=255)
                                     cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                           <  2:371375/149‑1 (MQ=255)
                                     cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                           <  3:1513097/149‑1 (MQ=255)
                                       ccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTg                                                                                                         >  2:929228/1‑149 (MQ=255)
                                       ccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTg                                                                                                         <  1:85805/149‑1 (MQ=255)
                                            gAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCAtt                                                                                                    <  1:1202464/149‑1 (MQ=255)
                                                tGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCAt                                                                                                <  1:675003/149‑1 (MQ=255)
                                                 gCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATc                                                                                               >  3:659015/1‑149 (MQ=255)
                                                      tGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGt                                                                                          <  4:406333/149‑1 (MQ=255)
                                                         gTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATg                                                                                       >  2:270829/1‑149 (MQ=255)
                                                           gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                     <  1:769968/149‑1 (MQ=255)
                                                           gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                     >  1:1696092/1‑149 (MQ=255)
                                                            aaCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCg                                                                                    <  1:1279072/149‑1 (MQ=255)
                                                             aCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGc                                                                                   <  3:1139968/149‑1 (MQ=255)
                                                                      cACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGc                                                                          >  2:7999/1‑149 (MQ=255)
                                                                           tACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGaa                                                                     >  3:413834/1‑149 (MQ=255)
                                                                            aCCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAg                                                                    >  3:645088/1‑149 (MQ=255)
                                                                             ccgccgACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGt                                                                   <  2:1603322/149‑1 (MQ=255)
                                                                                     ggATATTCAGCTTTATTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCac                                                                       <  4:1090310/137‑2 (MQ=255)
                                                                                     ggATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGAc                                                           >  2:484435/1‑149 (MQ=255)
                                                                                        tatTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGt                                                        >  1:1135358/1‑149 (MQ=255)
                                                                                         atTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTc                                                       <  2:1472852/149‑1 (MQ=255)
                                                                                          ttCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                      <  1:1353854/149‑1 (MQ=255)
                                                                                           tCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     <  1:989401/149‑1 (MQ=255)
                                                                                           tCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     >  1:1269846/1‑149 (MQ=255)
                                                                                             aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                   <  1:109891/149‑1 (MQ=255)
                                                                                             aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                   <  4:659015/149‑1 (MQ=255)
                                                                                                tttACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCa                                                >  2:531494/1‑149 (MQ=255)
                                                                                                  tACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAcc                                              >  3:149429/1‑149 (MQ=255)
                                                                                                   aCTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTgag                                                                                                                              <  4:394084/68‑1 (MQ=255)
                                                                                                     tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGa                                                                            <  3:854407/116‑1 (MQ=255)
                                                                                                     tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                           >  4:1208409/1‑149 (MQ=255)
                                                                                                     tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                           >  1:705278/1‑149 (MQ=255)
                                                                                                      aTCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAccc                                             >  4:334585/1‑146 (MQ=255)
                                                                                                      aTCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAccc                                             <  3:334585/146‑1 (MQ=255)
                                                                                                       tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  1:574413/1‑149 (MQ=255)
                                                                                                       tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  1:325227/1‑149 (MQ=255)
                                                                                                       tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  2:367360/1‑149 (MQ=255)
                                                                                                       tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  4:1352420/1‑149 (MQ=255)
                                                                                                       tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                         >  2:369476/1‑149 (MQ=255)
                                                                                                          gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                      >  1:7586/1‑133 (MQ=255)
                                                                                                          gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                      <  2:7586/133‑1 (MQ=255)
                                                                                                          gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCgcgc                                      >  1:344420/1‑149 (MQ=255)
                                                                                                             gCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCTGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCaa                                   <  3:1125744/149‑1 (MQ=255)
                                                                                                             gCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCaa                                   <  1:929228/149‑1 (MQ=255)
                                                                                                               tGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACg                                 <  3:1352420/149‑1 (MQ=255)
                                                                                                                gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCt                                                                              >  1:361222/1‑103 (MQ=255)
                                                                                                                gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                >  3:848793/1‑149 (MQ=255)
                                                                                                                gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                <  4:848793/149‑1 (MQ=255)
                                                                                                                gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                >  2:860937/1‑149 (MQ=255)
                                                                                                                 ttGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGc                               >  1:1708932/1‑149 (MQ=255)
                                                                                                                 ttGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGc                               >  3:1173612/1‑149 (MQ=255)
                                                                                                                  tGATTGGTCGCAAAGCATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTTCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                              <  2:1470857/149‑1 (MQ=255)
                                                                                                                  tGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                              <  1:1145747/149‑1 (MQ=255)
                                                                                                                   gATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAg                             <  4:1441352/149‑1 (MQ=255)
                                                                                                                      tGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTaa                          >  1:699617/1‑149 (MQ=255)
                                                                                                                         tCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGa                       <  1:1439908/149‑1 (MQ=255)
                                                                                                                            cAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGc                    >  1:78408/1‑149 (MQ=255)
                                                                                                                             aaaGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGcc                   >  4:1593010/1‑149 (MQ=255)
                                                                                                                                 aaTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     >  4:554464/1‑111 (MQ=255)
                                                                                                                                 aaTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                     <  3:554464/111‑1 (MQ=255)
                                                                                                                                  aTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAAccc              <  2:1135358/149‑1 (MQ=255)
                                                                                                                                   ttACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCa             >  1:1383330/1‑149 (MQ=255)
                                                                                                                                   ttACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCa             <  1:889132/149‑1 (MQ=255)
                                                                                                                                    tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg            <  1:1507175/149‑1 (MQ=255)
                                                                                                                                    tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg            <  3:1208409/149‑1 (MQ=255)
                                                                                                                                    tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg            <  1:860937/149‑1 (MQ=255)
                                                                                                                                      gcccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGt          >  4:534682/5‑149 (MQ=255)
                                                                                                                                      ccGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGt          <  4:645088/149‑1 (MQ=255)
                                                                                                                                       cGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTg         >  1:219947/1‑149 (MQ=255)
                                                                                                                                        ccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgc        >  4:1464832/3‑149 (MQ=255)
                                                                                                                                        ccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgc        >  3:223018/3‑149 (MQ=255)
                                                                                                                                         tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc       <  2:699617/149‑1 (MQ=255)
                                                                                                                                         tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGCTgcc       >  3:695643/1‑149 (MQ=255)
                                                                                                                                          aTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgccg      >  3:931464/1‑149 (MQ=255)
                                                                                                                                              gcCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCcgccgc  >  4:482148/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TACAACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGC  >  NC_000913/492975‑493265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑