Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 493,126 T→G 5.2% R345R (CGT→CGG dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913493,1260TG 5.2% 454.7 / 11.7 129R345R (CGT→CGGdnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base T (59/63);  new base G (5/2);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 131 total aligned reads displayed.

AACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAAT  >  NC_000913/492978‑493274
                                                                                                                                                    |                                                                                                                                                    
aaCTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGt                                                                                                                                                      >  1:1603322/1‑149 (MQ=255)
  cTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCg                                                                                                                                                    >  4:8032/1‑149 (MQ=255)
     tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                                 <  2:719536/149‑1 (MQ=255)
     tCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCAt                                                                                                                                                 <  2:109691/149‑1 (MQ=255)
         ctgctgCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGc                                                                                                                                             <  2:1594801/149‑1 (MQ=255)
         ctgctgCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGc                                                                                                                                             <  3:1506624/149‑1 (MQ=255)
           gctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGt                                                                                                                                           >  4:1139968/1‑149 (MQ=255)
            ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTATCTTATGTAATTGTCCGTCGCATggaccat                                                                                                                                          <  2:1321284/149‑6 (MQ=255)
            ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGtt                                                                                                                                          <  2:1429315/149‑1 (MQ=255)
            ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGACGGTCTGGCGCAtacggtaa                                                                                                                                          <  3:118455/149‑8 (MQ=255)
            ctgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATAACGCCGACGGATATTCAGCTTTACCATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGtt                                                                                                                                          <  1:1585914/149‑1 (MQ=255)
             tgcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTg                                                                                                                                         >  1:530092/1‑149 (MQ=255)
              gcACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTga                                                                                                                                        <  2:722721/149‑1 (MQ=255)
                 cTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGAt                                                                                                                                     <  2:875494/149‑1 (MQ=255)
                   tGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGa                                                                                                                                   <  1:80165/149‑1 (MQ=255)
                    ggCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGAc                                                                                                                                  <  1:1568502/149‑1 (MQ=255)
                              tGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgc                                                                                                                        <  2:530092/149‑1 (MQ=255)
                               ggCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcg                                                                                                                       >  2:1439908/1‑149 (MQ=255)
                                 ccgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcg                                                                                                                     >  4:731416/1‑149 (MQ=255)
                                  cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                                    <  3:968945/149‑1 (MQ=255)
                                  cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                                    <  2:371375/149‑1 (MQ=255)
                                  cgccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTgcgcgcgc                                                                                                                    <  3:1513097/149‑1 (MQ=255)
                                    ccATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTg                                                                                                                  <  1:85805/149‑1 (MQ=255)
                                         gAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCAtt                                                                                                             <  4:425115/149‑1 (MQ=255)
                                         gAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCAtt                                                                                                             <  1:1202464/149‑1 (MQ=255)
                                              gCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATc                                                                                                        >  3:659015/1‑149 (MQ=255)
                                                      gTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATg                                                                                                >  4:1125744/1‑149 (MQ=255)
                                                      gTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATg                                                                                                >  2:270829/1‑149 (MQ=255)
                                                       tGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGc                                                                                               >  3:770562/1‑149 (MQ=255)
                                                        gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                              <  1:769968/149‑1 (MQ=255)
                                                        gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                              >  1:1696092/1‑149 (MQ=255)
                                                        gAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGcc                                                                                              >  3:9452/1‑149 (MQ=255)
                                                         aaCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCg                                                                                             <  1:1279072/149‑1 (MQ=255)
                                                          aCTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGc                                                                                            <  3:1139968/149‑1 (MQ=255)
                                                              gcgcgcACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATgg                                                                                                                                               >  4:558015/1‑94 (MQ=255)
                                                              gcgcgcACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGAATgg                                                                                                                                               <  3:558015/94‑1 (MQ=255)
                                                                   cACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGc                                                                                   <  2:816649/149‑1 (MQ=255)
                                                                   cACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGc                                                                                   >  2:7999/1‑149 (MQ=255)
                                                                        tACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGaa                                                                              <  4:1401172/149‑1 (MQ=255)
                                                                        tACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGaa                                                                              >  3:413834/1‑149 (MQ=255)
                                                                         aCCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAg                                                                             >  3:645088/1‑149 (MQ=255)
                                                                          ccgccgACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGt                                                                            <  2:1603322/149‑1 (MQ=255)
                                                                               gACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCAc                                                                       <  4:169087/149‑1 (MQ=255)
                                                                                  ggATATTCAGCTTTATTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCac                                                                                <  4:1090310/137‑2 (MQ=255)
                                                                                  ggATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAg                                                                                >  3:1090310/1‑137 (MQ=255)
                                                                                   gATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACa                                                                   >  2:1098195/1‑149 (MQ=255)
                                                                                     tatTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGt                                                                 >  1:1135358/1‑149 (MQ=255)
                                                                                      atTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTc                                                                <  2:1472852/149‑1 (MQ=255)
                                                                                       ttCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                               <  1:1353854/149‑1 (MQ=255)
                                                                                        tCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                              >  1:1269846/1‑149 (MQ=255)
                                                                                          aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                            <  4:659015/149‑1 (MQ=255)
                                                                                          aGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCttt                                                            <  1:109891/149‑1 (MQ=255)
                                                                                             tttACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCa                                                         >  2:531494/1‑149 (MQ=255)
                                                                                                aCTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTgag                                                                                                                                       <  4:394084/68‑1 (MQ=255)
                                                                                                aCTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTgag                                                                                                                                       >  3:394084/1‑68 (MQ=255)
                                                                                                  tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGa                                                                                     <  3:854407/116‑1 (MQ=255)
                                                                                                  tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                                    >  1:705278/1‑149 (MQ=255)
                                                                                                  tATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                                    >  4:1208409/1‑149 (MQ=255)
                                                                                                   aTCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAccc                                                      >  4:334585/1‑146 (MQ=255)
                                                                                                   aTCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCAccc                                                      <  3:334585/146‑1 (MQ=255)
                                                                                                    tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                                  >  2:369476/1‑149 (MQ=255)
                                                                                                    tCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTcg                                                  >  1:574413/1‑149 (MQ=255)
                                                                                                       gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTcc                                                               <  2:7586/133‑1 (MQ=255)
                                                                                                       gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCgcgc                                               >  1:344420/1‑149 (MQ=255)
                                                                                                       gACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCgcgc                                               <  1:270829/149‑1 (MQ=255)
                                                                                                          gCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCTGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCaa                                            <  3:1125744/149‑1 (MQ=255)
                                                                                                          gCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCaa                                            <  1:929228/149‑1 (MQ=255)
                                                                                                            tGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACg                                          <  3:1352420/149‑1 (MQ=255)
                                                                                                             gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCt                                                                                       >  1:361222/1‑103 (MQ=255)
                                                                                                             gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCt                                                                                       <  2:361222/103‑1 (MQ=255)
                                                                                                             gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                         >  3:848793/1‑149 (MQ=255)
                                                                                                             gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                         <  4:848793/149‑1 (MQ=255)
                                                                                                             gTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACgg                                         >  2:860937/1‑149 (MQ=255)
                                                                                                              ttGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGc                                        >  1:1708932/1‑149 (MQ=255)
                                                                                                               tGATTGGTCGCAAAGCATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTTCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                                       <  2:1470857/149‑1 (MQ=255)
                                                                                                               tGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCa                                       <  1:1145747/149‑1 (MQ=255)
                                                                                                                gATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAg                                      <  4:1441352/149‑1 (MQ=255)
                                                                                                                gATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAg                                      >  1:916319/1‑149 (MQ=255)
                                                                                                                  ttGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTa                                    >  1:757392/1‑149 (MQ=255)
                                                                                                                   tGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTaa                                   >  1:699617/1‑149 (MQ=255)
                                                                                                                      tCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGa                                <  1:1439908/149‑1 (MQ=255)
                                                                                                                         cAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGc                             >  1:78408/1‑149 (MQ=255)
                                                                                                                          aaaGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGcc                            >  4:1593010/1‑149 (MQ=255)
                                                                                                                              aaTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCt                                                              <  3:554464/111‑1 (MQ=255)
                                                                                                                               aTTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAAccc                       <  2:1135358/149‑1 (MQ=255)
                                                                                                                                ttACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCa                      >  1:1383330/1‑149 (MQ=255)
                                                                                                                                ttACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCa                      <  1:889132/149‑1 (MQ=255)
                                                                                                                                 tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg                     <  3:1208409/149‑1 (MQ=255)
                                                                                                                                 tACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAg                     <  1:1507175/149‑1 (MQ=255)
                                                                                                                                   gcccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGt                   >  4:534682/5‑149 (MQ=255)
                                                                                                                                   ccGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGt                   <  4:645088/149‑1 (MQ=255)
                                                                                                                                   acGTATGCTCCGGACCGGCGCATGGGCGTTGGGATGACGGTGCTGCCCGGGATGGCATTCCACCCGCGTAAGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTACTTTGCTCACGTAGCGCCAACGGTAGTAATGACGCCAACCCAGGt                   <  2:413243/148‑1 (MQ=255)
                                                                                                                                    cGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTg                  >  1:219947/1‑149 (MQ=255)
                                                                                                                                     ccaTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgc                 >  4:1464832/3‑149 (MQ=255)
                                                                                                                                      tATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc                >  2:670999/1‑149 (MQ=255)
                                                                                                                                      caTGCGACGGTCCGGCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgcc                >  1:614865/2‑149 (MQ=255)
                                                                                                                                         gcgcCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTgccgcc             <  1:446004/149‑1 (MQ=255)
                                                                                                                                           gcCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCcgccgc           >  4:482148/1‑149 (MQ=255)
                                                                                                                                                  cGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGt                                                    <  3:1677135/101‑1 (MQ=255)
                                                                                                                                                    tCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAAt  <  2:1383330/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AACTTTCGCCTGCTGCACTTGGCAACGACATGGCCGCCATCGAGCTGCGGATGCGTGAACTGGCGCGCACCATACCGCCGACGGATATTCAGCTTTACTATCAGACGCTGTTGATTGGTCGCAAAGAATTACCGTATGCGCCGGACCGTCGCATGGGCGTTGAGATGACGCTGCTGCGCGCGCTGGCATTCCATCCGCGTATGCCGCTGCCTGAGCCAGAAGTGCCACGACAGTCCTTTGCACCCGTCGCGCCAACGGCAGTAATGACGCCAACCCAGGTGCCGCCGCAACCGCAAT  >  NC_000913/492978‑493274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: