Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,106,992 G→A 5.9% intergenic (+22/+28) nupG → / ← speC nucleoside transporter/ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,106,9920GA 5.9% 520.2 / 11.0 162intergenic (+22/+28)nupG/speCnucleoside transporter/ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (78/73);  new base A (3/7);  total (81/81)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 165 total aligned reads displayed.

ACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAA  >  NC_000913/3106845‑3107140
                                                                                                                                                   |                                                                                                                                                     
  cGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                                                                                                                                    >  3:1023444/1‑149 (MQ=255)
  cGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                                                                                                                                    >  1:1294711/1‑149 (MQ=255)
      tGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAg                                                                                                                                                >  2:1123615/1‑149 (MQ=255)
        gCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                              >  1:422071/1‑149 (MQ=255)
           gACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGAc                                                                                                                                          <  4:184094/149‑1 (MQ=255)
                tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtt                                                                                                                                     <  3:417035/149‑1 (MQ=255)
                 aTGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGttt                                                                                                                                    <  1:175244/149‑1 (MQ=255)
                    gTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                                                                                                                                 >  2:702212/1‑149 (MQ=255)
                    gTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                                                                                                                                 >  3:697166/1‑149 (MQ=255)
                     ttGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGttttttt                                                                                                                                >  4:867636/1‑149 (MQ=255)
                      tGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                               <  1:222170/149‑1 (MQ=39)
                       gATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTa                                                                                                                              <  4:864874/149‑1 (MQ=255)
                       gATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTa                                                                                                                              <  4:251254/149‑1 (MQ=255)
                        aTTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCTCTAATGTCGCAAAGATAAACGGGTCACCTTCTGGCGTCCCGtttttctttg                                                                                                                             <  2:780343/149‑3 (MQ=255)
                        aTTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTAt                                                                                                                             <  2:747984/149‑1 (MQ=255)
                          tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTc                                                                                                                           <  4:697166/149‑1 (MQ=255)
                            tCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCtt                                                                                                                         <  3:966242/149‑1 (MQ=255)
                             cGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTa                                                                                                                        <  3:710489/149‑1 (MQ=255)
                              gCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCTACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTAc                                                                                                                       <  4:1325818/149‑1 (MQ=255)
                               cTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                               <  3:880953/140‑1 (MQ=38)
                               cTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                               >  4:880953/1‑140 (MQ=38)
                                tGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                                                                                                                     >  3:136615/1‑149 (MQ=255)
                                tGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                                                                                                                     >  4:61218/1‑149 (MQ=255)
                                   ttACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCaa                                                                                                                  >  2:724458/1‑149 (MQ=255)
                                    tACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac                                                                                                                 <  2:56700/149‑1 (MQ=255)
                                    tACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac                                                                                                                 >  2:398202/1‑149 (MQ=255)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGaa                                                                                                                                                 >  4:468656/1‑115 (MQ=255)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGa                                                                                                                                            >  2:825044/1‑120 (MQ=255)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                              >  1:1543614/1‑149 (MQ=255)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                              >  2:1612751/1‑149 (MQ=255)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctttgc                                                                                                                            >  3:1529129/1‑132 (MQ=38)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctttgc                                                                                                                            <  4:1529129/135‑4 (MQ=38)
                                       tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGACGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                              >  4:331209/1‑149 (MQ=255)
                                           tGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAAc                                                                                                          <  4:1023444/149‑1 (MQ=255)
                                                 tGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACa                                                                                                    >  4:1286144/1‑149 (MQ=255)
                                                   gCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAAc                                                                                                  >  4:1140389/1‑149 (MQ=255)
                                                     cTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                              >  3:1659939/1‑104 (MQ=255)
                                                     cTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCg                                                                                                >  1:261379/1‑149 (MQ=255)
                                                      ttCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGt                                                                                               >  1:1529215/1‑149 (MQ=255)
                                                      ttCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGt                                                                                               >  2:1527024/1‑149 (MQ=255)
                                                       tCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGtt                                                                                              <  3:299108/149‑1 (MQ=255)
                                                          cgTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCa                                                                                           <  1:175983/149‑1 (MQ=255)
                                                           gTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                              <  1:101823/98‑1 (MQ=255)
                                                           gTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                              >  2:101823/1‑98 (MQ=255)
                                                             tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                        <  4:1380008/149‑1 (MQ=255)
                                                             tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                        <  3:1162069/149‑1 (MQ=255)
                                                             tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                        >  3:107647/1‑149 (MQ=255)
                                                             tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                        <  2:637144/149‑1 (MQ=255)
                                                                 ggCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCTACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATc                                                                                    <  1:1440931/149‑1 (MQ=255)
                                                                        ttCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCg                                                                             <  2:377238/149‑1 (MQ=255)
                                                                           aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                          <  1:398202/149‑1 (MQ=255)
                                                                           aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                          <  1:724458/149‑1 (MQ=255)
                                                                           aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                          >  4:1263983/1‑149 (MQ=255)
                                                                           aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                          >  2:350891/1‑149 (MQ=255)
                                                                            aaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGGCCCGTCTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGttt                                                                         <  3:976672/149‑1 (MQ=255)
                                                                             atatAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTc                                                                        <  4:294339/149‑1 (MQ=255)
                                                                               ataAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGc                                                                      <  2:284915/149‑1 (MQ=255)
                                                                               ataAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGc                                                                      <  4:136615/149‑1 (MQ=255)
                                                                                taAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCt                                                                     <  3:1233671/149‑1 (MQ=255)
                                                                                taAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCt                                                                     <  1:36969/149‑1 (MQ=255)
                                                                                  aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                   <  2:1447156/149‑1 (MQ=255)
                                                                                  aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                   >  2:250706/1‑149 (MQ=255)
                                                                                  aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                   >  3:514816/1‑149 (MQ=255)
                                                                                   acacGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTAc                                                                                                     <  3:810059/114‑1 (MQ=255)
                                                                                       gTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAaca                                                              >  3:1004428/1‑149 (MQ=255)
                                                                                           gtgtCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCtt                                                          <  1:1060328/149‑1 (MQ=255)
                                                                                           gtgtCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCtt                                                          <  1:1669571/149‑1 (MQ=255)
                                                                                              tCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTc                                                                                                                           <  3:639786/81‑1 (MQ=255)
                                                                                              tCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCa                                                       >  3:274692/1‑149 (MQ=255)
                                                                                                ccGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGc                                                     >  1:993212/1‑149 (MQ=255)
                                                                                                 cGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCt                                                    >  4:1115418/1‑149 (MQ=255)
                                                                                                  gACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTc                                                   >  2:1720901/1‑149 (MQ=255)
                                                                                                       gCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCg                                              >  3:558439/1‑149 (MQ=255)
                                                                                                         acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAGCCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                            <  3:346618/149‑1 (MQ=255)
                                                                                                         acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                            >  2:1682219/1‑149 (MQ=255)
                                                                                                         acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTCTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                            >  4:326949/1‑149 (MQ=255)
                                                                                                           acaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaaa                                          >  4:1634010/1‑149 (MQ=255)
                                                                                                              gACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCACCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATcc                                       <  1:1011406/149‑1 (MQ=255)
                                                                                                                 ggTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCgg                                    >  2:844384/1‑149 (MQ=255)
                                                                                                                       ccACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGcaacaa                              >  2:413103/1‑149 (MQ=255)
                                                                                                                        cACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACaaa                             <  4:717560/149‑1 (MQ=255)
                                                                                                                             ttaaCGCAAAAAAAACCGGTCCGCCAAAGGTTACCCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAc                        <  2:120121/146‑1 (MQ=255)
                                                                                                                             aTTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAc                        <  2:1294711/149‑1 (MQ=255)
                                                                                                                             aTTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGATTCGCTATAAACACCTTGCAGCTCCGGc                                               <  3:1447713/126‑1 (MQ=255)
                                                                                                                              ttACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAcc                       <  1:273519/149‑1 (MQ=255)
                                                                                                                                    aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                 >  3:1476865/1‑149 (MQ=255)
                                                                                                                                    aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                 >  4:1499380/1‑149 (MQ=255)
                                                                                                                                    aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                 >  4:1432238/1‑149 (MQ=255)
                                                                                                                                     aaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCAc                                                                                          <  4:744849/75‑1 (MQ=255)
                                                                                                                                     aaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttctt                >  1:911062/1‑149 (MQ=255)
                                                                                                                                       aaaaaaCGGGTCACCTTCTGGCGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGc                                                        <  4:1312619/106‑1 (MQ=37)
                                                                                                                                       aaaaaaCGGGTCACCTTCTGGCGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTcc              >  3:997789/2‑149 (MQ=255)
                                                                                                                                        aaaaaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCa             <  3:326949/149‑1 (MQ=255)
                                                                                                                                        aaaaaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCa             <  1:751870/149‑1 (MQ=255)
                                                                                                                                           aaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaa                                           <  2:669394/116‑1 (MQ=255)
                                                                                                                                           aaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaa                                           >  1:669394/1‑116 (MQ=255)
                                                                                                                                              gggTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCaa       <  4:1476865/149‑1 (MQ=255)
                                                                                                                                                gTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAgg     <  4:721108/149‑1 (MQ=255)
                                                                                                                                                 tCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACACAACCGAACAACAGTTGCACGCTATCCGCTTAGGTTTCGCTATAAACACCTTTCCGCTCCGGCGACAATCCCGGCGACTAATTCACCCCTTCTTCCAGTCCAAGGa    >  4:225476/1‑149 (MQ=255)
                                                                                                                                                   gCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGaaa  >  4:393539/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACCGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAA  >  NC_000913/3106845‑3107140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: