Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,106,995 A→T 6.7% intergenic (+25/+25) nupG → / ← speC nucleoside transporter/ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,106,9950AT 6.7% 568.3 / 20.6 163intergenic (+25/+25)nupG/speCnucleoside transporter/ornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base A (78/73);  new base T (3/8);  total (82/81)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 165 total aligned reads displayed.

CGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATA  >  NC_000913/3106847‑3107142
                                                                                                                                                    |                                                                                                                                                    
cGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCa                                                                                                                                                      >  3:1023444/1‑149 (MQ=255)
    tGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAg                                                                                                                                                  >  2:1123615/1‑149 (MQ=255)
      gCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                                >  1:422071/1‑149 (MQ=255)
         gACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGAc                                                                                                                                            <  4:184094/149‑1 (MQ=255)
              tATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtt                                                                                                                                       <  3:417035/149‑1 (MQ=255)
               aTGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGttt                                                                                                                                      <  1:175244/149‑1 (MQ=255)
                  gTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                                                                                                                                   >  2:702212/1‑149 (MQ=255)
                  gTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGtttttt                                                                                                                                   >  3:697166/1‑149 (MQ=255)
                   ttGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGttttttt                                                                                                                                  >  4:867636/1‑149 (MQ=255)
                    tGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                                 <  1:222170/149‑1 (MQ=39)
                     gATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTa                                                                                                                                <  4:864874/149‑1 (MQ=255)
                     gATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTa                                                                                                                                <  4:251254/149‑1 (MQ=255)
                      aTTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCTCTAATGTCGCAAAGATAAACGGGTCACCTTCTGGCGTCCCGtttttctttg                                                                                                                               <  2:780343/149‑3 (MQ=255)
                      aTTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTAt                                                                                                                               <  2:747984/149‑1 (MQ=255)
                        tttCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTc                                                                                                                             <  4:697166/149‑1 (MQ=255)
                          tCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCtt                                                                                                                           <  3:966242/149‑1 (MQ=255)
                           cGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTa                                                                                                                          <  3:710489/149‑1 (MQ=255)
                            gCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCTACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTAc                                                                                                                         <  4:1325818/149‑1 (MQ=255)
                             cTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                                 <  3:880953/140‑1 (MQ=38)
                             cTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctt                                                                                                                                 >  4:880953/1‑140 (MQ=38)
                              tGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                                                                                                                       >  3:136615/1‑149 (MQ=255)
                              tGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACtt                                                                                                                       >  4:61218/1‑149 (MQ=255)
                                 ttACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCaa                                                                                                                    >  2:724458/1‑149 (MQ=255)
                                  tACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac                                                                                                                   <  2:56700/149‑1 (MQ=255)
                                  tACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAac                                                                                                                   >  2:398202/1‑149 (MQ=255)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGaa                                                                                                                                                   >  4:468656/1‑115 (MQ=255)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGa                                                                                                                                              >  2:825044/1‑120 (MQ=255)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                                >  2:1612751/1‑149 (MQ=255)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                                >  1:1447156/1‑149 (MQ=255)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctttgc                                                                                                                              >  3:1529129/1‑132 (MQ=38)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCACCTTCTGGCGACCCGtttttctttgc                                                                                                                              <  4:1529129/135‑4 (MQ=38)
                                     tCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGACGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAAcaca                                                                                                                >  4:331209/1‑149 (MQ=255)
                                         tGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAAc                                                                                                            <  4:1023444/149‑1 (MQ=255)
                                               tGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACa                                                                                                      >  4:1286144/1‑149 (MQ=255)
                                                 gCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAAc                                                                                                    >  4:1140389/1‑149 (MQ=255)
                                                   cTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                                >  3:1659939/1‑104 (MQ=255)
                                                   cTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCg                                                                                                  >  1:261379/1‑149 (MQ=255)
                                                    ttCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGt                                                                                                 >  2:1527024/1‑149 (MQ=255)
                                                    ttCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGt                                                                                                 >  1:1529215/1‑149 (MQ=255)
                                                     tCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGtt                                                                                                <  3:299108/149‑1 (MQ=255)
                                                         gTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                                <  1:101823/98‑1 (MQ=255)
                                                         gTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGt                                                                                                                                                >  2:101823/1‑98 (MQ=255)
                                                           tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                          <  2:637144/149‑1 (MQ=255)
                                                           tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                          >  3:107647/1‑149 (MQ=255)
                                                           tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                          <  3:1162069/149‑1 (MQ=255)
                                                           tCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGc                                                                                          <  4:1380008/149‑1 (MQ=255)
                                                                      ttCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCg                                                                               <  2:377238/149‑1 (MQ=255)
                                                                         aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                            >  4:1263983/1‑149 (MQ=255)
                                                                         aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                            >  2:350891/1‑149 (MQ=255)
                                                                         aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                            <  1:724458/149‑1 (MQ=255)
                                                                         aaaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGtt                                                                            <  1:398202/149‑1 (MQ=255)
                                                                          aaTATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGGCCCGTCTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGttt                                                                           <  3:976672/149‑1 (MQ=255)
                                                                           atatAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTc                                                                          <  4:294339/149‑1 (MQ=255)
                                                                             ataAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGc                                                                        <  2:284915/149‑1 (MQ=255)
                                                                             ataAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGc                                                                        <  4:136615/149‑1 (MQ=255)
                                                                              taAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCt                                                                       <  3:1233671/149‑1 (MQ=255)
                                                                              taAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCt                                                                       <  1:36969/149‑1 (MQ=255)
                                                                                aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                     >  3:514816/1‑149 (MQ=255)
                                                                                aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                     <  2:1447156/149‑1 (MQ=255)
                                                                                aaCACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCtat                                                                     >  2:250706/1‑149 (MQ=255)
                                                                                 acacGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTAc                                                                                                       <  3:810059/114‑1 (MQ=255)
                                                                                     gTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAaca                                                                >  3:1004428/1‑149 (MQ=255)
                                                                                         gtgtCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCtt                                                            <  1:1060328/149‑1 (MQ=255)
                                                                                            tCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTc                                                                                                                             <  3:639786/81‑1 (MQ=255)
                                                                                            tCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCa                                                         >  3:274692/1‑149 (MQ=255)
                                                                                              ccGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGc                                                       >  1:993212/1‑149 (MQ=255)
                                                                                               cGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCt                                                      >  4:1115418/1‑149 (MQ=255)
                                                                                                gACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTc                                                     >  2:1720901/1‑149 (MQ=255)
                                                                                                     gCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCg                                                >  3:558439/1‑149 (MQ=255)
                                                                                                       acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAGCCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                              <  3:346618/149‑1 (MQ=255)
                                                                                                       acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                              >  2:1682219/1‑149 (MQ=255)
                                                                                                       acacaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTCTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaa                                              >  4:326949/1‑149 (MQ=255)
                                                                                                         acaGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaaa                                            >  4:1634010/1‑149 (MQ=255)
                                                                                                          caGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAAt                                           >  1:1556566/1‑149 (MQ=255)
                                                                                                            gACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCACCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATcc                                         <  1:1011406/149‑1 (MQ=255)
                                                                                                               ggTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCgg                                      >  2:844384/1‑149 (MQ=255)
                                                                                                                     ccACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGcaacaa                                >  1:1350305/1‑149 (MQ=255)
                                                                                                                     ccACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGcaacaa                                >  2:413103/1‑149 (MQ=255)
                                                                                                                      cACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACaaa                               <  4:717560/149‑1 (MQ=255)
                                                                                                                           ttaaCGCAAAAAAAACCGGTCCGCCAAAGGTTACCCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAc                          <  2:120121/146‑1 (MQ=255)
                                                                                                                           aTTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAc                          <  2:1294711/149‑1 (MQ=255)
                                                                                                                           aTTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGATTCGCTATAAACACCTTGCAGCTCCGGc                                                 <  3:1447713/126‑1 (MQ=255)
                                                                                                                            ttACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCAcc                         <  1:273519/149‑1 (MQ=255)
                                                                                                                                  aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                   >  4:1499380/1‑149 (MQ=255)
                                                                                                                                  aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                   >  4:1432238/1‑149 (MQ=255)
                                                                                                                                  aaaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttct                   >  3:1476865/1‑149 (MQ=255)
                                                                                                                                   aaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCAc                                                                                            <  4:744849/75‑1 (MQ=255)
                                                                                                                                   aaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttctt                  >  1:1713052/1‑149 (MQ=255)
                                                                                                                                   aaGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCcttctt                  >  1:911062/1‑149 (MQ=255)
                                                                                                                                     aaaaaaCGGGTCACCTTCTGGCGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGc                                                          <  4:1312619/106‑1 (MQ=37)
                                                                                                                                     aaaaaaCGGGTCACCTTCTGGCGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTcc                >  3:997789/2‑149 (MQ=255)
                                                                                                                                      aaaaaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCa               <  3:326949/149‑1 (MQ=255)
                                                                                                                                      aaaaaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCa               <  1:751870/149‑1 (MQ=255)
                                                                                                                                         aaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaa                                             >  1:669394/1‑116 (MQ=255)
                                                                                                                                         aaCGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGaaa                                             <  2:669394/116‑1 (MQ=255)
                                                                                                                                            gggTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCaa         <  4:1476865/149‑1 (MQ=255)
                                                                                                                                              gTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAgg       <  4:721108/149‑1 (MQ=255)
                                                                                                                                               tCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACACAACCGAACAACAGTTGCACGCTATCCGCTTAGGTTTCGCTATAAACACCTTTCCGCTCCGGCGACAATCCCGGCGACTAATTCACCCCTTCTTCCAGTCCAAGGa      >  4:225476/1‑149 (MQ=255)
                                                                                                                                                 gCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGaaa    >  4:393539/1‑149 (MQ=255)
                                                                                                                                                   cAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATa  >  1:126768/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGACTGGCAGACCGTATGGTTGATTTTCGCTGGTTACTCCGTGGTTCTGGCCTTCGCGTTCATGGCGATGTTCAAATATAAACACGTTCGTGTCCCGACAGGCACACAGACGGTTAGCCACTAATTACGCAAAGAAAAACGGGTCGCCAGAAGGTGACCCGTTTTTTTTATTCTTACTTCAACACATAACCGTACAACCGTTTCACGCCATCCGCATCGGTTTCGCTATAAACACCTTGCAGCTCCGGCGAAAATCCCGGCAACAAATTCACCCCTTCTTCCAGTGCAAGGAAATA  >  NC_000913/3106847‑3107142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: