Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,271,825 T→A 7.3% intergenic (‑55/+42) garK ← / ← garR glycerate kinase I/tartronate semialdehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,271,8250TA 7.3% 554.5 / 25.4 162intergenic (‑55/+42)garK/garRglycerate kinase I/tartronate semialdehyde reductase
Reads supporting (aligned to +/- strand):  ref base T (64/86);  new base A (8/4);  total (72/90)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 167 total aligned reads displayed.

GAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAA  >  NC_000913/3271677‑3271973
                                                                                                                                                     |                                                                                                                                                    
gAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATGACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGAt                                                                                                                                                      >  3:1009841/1‑149 (MQ=255)
 aaaaaTTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGAtt                                                                                                                                                     >  3:900666/1‑149 (MQ=255)
  aaaaTTTCCCGAAATCCTTTTCCTATCGCTTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACCATTTTCATACCTATGCCTGTTACCACATGACGCGGGAGGGCGTTTCTCTTATTCCGCCTGGATTc                                                                                                                                                    <  3:1407834/149‑1 (MQ=255)
   aaaTTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTcc                                                                                                                                                   <  3:1142629/149‑1 (MQ=255)
     aTTTCCCGAAATCCTTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCa                                                                                                                                                  <  2:67234/149‑1 (MQ=255)
     aTTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAg                                                                                                                                                 >  1:622166/1‑149 (MQ=255)
          ccGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCg                                                                                                                                            >  3:1116113/1‑149 (MQ=255)
            gAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGa                                                                                                                                          >  4:1550061/1‑149 (MQ=255)
            gAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGCCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGa                                                                                                                                          >  4:772611/1‑149 (MQ=255)
               aTCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTg                                                                                                                                       <  3:362474/149‑1 (MQ=255)
                tCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATGAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGc                                                                                                                                      <  1:844058/149‑1 (MQ=255)
                tCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGc                                                                                                                                      <  3:1430250/149‑1 (MQ=255)
                 ccTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCa                                                                                                                                     <  2:225110/149‑1 (MQ=255)
                  cTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCaa                                                                                                                                    >  1:557090/1‑149 (MQ=255)
                    tttttCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAAt                                                                                                                                   >  1:543761/1‑149 (MQ=255)
                     ttttCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATa                                                                                                                                  <  2:492966/149‑1 (MQ=255)
                        tCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGc                                                                                                                               <  4:1391156/149‑1 (MQ=255)
                          tATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGGGCGCACGATTTTCATACCTATGCCTGTTACCACATGACGCCGTAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATCCGCCa                                                                                                                             >  2:1023130/1‑149 (MQ=255)
                            tCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATc                                                                                                                           <  3:1550061/149‑1 (MQ=255)
                               ccTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCgg                                                                                                                        <  2:66737/149‑1 (MQ=255)
                                cTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCggg                                                                                                                       <  4:1116113/149‑1 (MQ=255)
                                   cgcAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCac                                                                                                                    >  4:227921/1‑149 (MQ=255)
                                     cAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTAGCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCacga                                                                                                                  >  1:31582/1‑149 (MQ=255)
                                        ccTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGt                                                                                                               >  1:223277/1‑149 (MQ=255)
                                         cTCGCTGGCAGGTAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGTCCAGGATTCCAGGCCCGGATTGCGATACGCCATCCGGGCACGACGTc                                                                                                              >  4:1477140/1‑149 (MQ=255)
                                          tCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACGTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCa                                                                                                             >  2:1475284/1‑149 (MQ=255)
                                          tCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCa                                                                                                             >  4:1062134/1‑149 (MQ=255)
                                               ggCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAAc                                                                                                        <  2:1473038/149‑1 (MQ=255)
                                               ggCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAAc                                                                                                        <  1:505567/149‑1 (MQ=255)
                                                 cAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACTCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      <  3:1224776/149‑1 (MQ=255)
                                                 cAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      <  1:962856/149‑1 (MQ=255)
                                                 cAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      <  4:1453941/149‑1 (MQ=255)
                                                 cAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      <  4:1646380/149‑1 (MQ=255)
                                                 cAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      <  3:670497/149‑1 (MQ=255)
                                                  aGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAg                                                                                                     >  4:1115558/1‑149 (MQ=255)
                                                     tAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTaa                                                                                                  <  2:300267/149‑1 (MQ=255)
                                                          tttctttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCg                                                                                             >  1:798312/1‑149 (MQ=255)
                                                            tctttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGAc                                                                                           <  4:1619810/149‑1 (MQ=255)
                                                              tttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGCCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACtt                                                                                         >  1:1032068/1‑149 (MQ=255)
                                                              tttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGAATCCAGGc                                                                                                                                               >  1:1028596/1‑95 (MQ=255)
                                                              tttATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGAATCCAGGc                                                                                                                                               <  2:1028596/95‑1 (MQ=255)
                                                                 ataAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCg                                                                                      >  2:1203306/1‑149 (MQ=255)
                                                                     gagTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAg                                                                                  <  4:1202684/149‑1 (MQ=255)
                                                                           ggggCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTc                                                                            <  3:599910/149‑1 (MQ=255)
                                                                           ggggCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTc                                                                            <  2:1244975/149‑1 (MQ=255)
                                                                            gggCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCg                                                                           >  3:245315/1‑149 (MQ=255)
                                                                              gCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgta                                                                         <  3:1681238/149‑1 (MQ=255)
                                                                              gCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgta                                                                         <  3:127183/149‑1 (MQ=255)
                                                                              gCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgta                                                                         <  1:1688921/149‑1 (MQ=255)
                                                                              gCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgta                                                                         <  4:1549324/149‑1 (MQ=255)
                                                                                 aTTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgtagta                                                                      >  3:1623698/1‑149 (MQ=255)
                                                                                      gATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAAc                                                                                                        >  3:1685589/1‑110 (MQ=255)
                                                                                      gATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACg                                                                 >  2:1070122/1‑149 (MQ=255)
                                                                                           tCATACCTATGCCTGTTAGCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAgg                                                            <  2:31582/149‑1 (MQ=255)
                                                                                               aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcgc                                                        <  1:649877/149‑1 (MQ=255)
                                                                                               aCCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcgc                                                        <  4:1308078/149‑1 (MQ=255)
                                                                                                ccTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCt                                                       <  3:5423/149‑1 (MQ=255)
                                                                                                 cTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGt                                                                                                               >  2:1418669/1‑92 (MQ=255)
                                                                                                 cTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGAc                                                                                           <  2:1685072/112‑1 (MQ=255)
                                                                                                 cTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGAc                                                                                           >  1:1685072/1‑112 (MQ=255)
                                                                                                 cTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTa                                                      <  1:300729/149‑1 (MQ=255)
                                                                                                 cTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTa                                                      >  1:1563473/1‑149 (MQ=255)
                                                                                                  tATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTAt                                                     <  4:1092452/149‑1 (MQ=255)
                                                                                                  tATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTAt                                                     >  4:3036/1‑149 (MQ=255)
                                                                                                    tGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCTTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGa                                                   <  2:1034306/149‑1 (MQ=255)
                                                                                                     gCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGAt                                                  <  3:505253/149‑1 (MQ=255)
                                                                                                       cTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGa                                                                                                      >  3:1085910/1‑95 (MQ=255)
                                                                                                       cTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGa                                                                                            >  4:281034/1‑105 (MQ=255)
                                                                                                       cTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATcc                                                >  1:1013508/1‑149 (MQ=255)
                                                                                                        tGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATccg                                               >  2:188515/1‑149 (MQ=255)
                                                                                                            aCCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGt                                           >  1:1600300/1‑149 (MQ=255)
                                                                                                             ccACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGtt                                          <  3:783761/149‑1 (MQ=255)
                                                                                                              cacaTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTc                                         >  1:120905/1‑149 (MQ=255)
                                                                                                              cacaTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTc                                         <  3:1115558/149‑1 (MQ=255)
                                                                                                               acaTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                        >  1:640308/1‑149 (MQ=255)
                                                                                                                 aTGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGttttt                                                                             >  3:947887/1‑110 (MQ=255)
                                                                                                                   gACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaaa                                    <  1:1345575/149‑1 (MQ=255)
                                                                                                                     cGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAcc                                  <  2:622166/149‑1 (MQ=255)
                                                                                                                         ggAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCt                              <  2:798312/149‑1 (MQ=255)
                                                                                                                              gCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCg                         >  1:1159848/1‑149 (MQ=255)
                                                                                                                               cGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCg                                                                           >  4:51367/1‑98 (MQ=255)
                                                                                                                                gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGtttt                                                                              >  2:1299582/1‑94 (MQ=255)
                                                                                                                                gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGtttt                                                                              <  1:1299582/94‑1 (MQ=255)
                                                                                                                                gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCgtag                                                                        <  1:1436173/100‑1 (MQ=255)
                                                                                                                                gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                       >  1:1103337/1‑149 (MQ=255)
                                                                                                                                 tttCTCTTATTCGGCCTGCATTCCAAGCCCGGTTTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCAACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTTTGATCCGCCGTTCCTAAACCATCTGCTCGCaa                      >  2:1209175/1‑148 (MQ=255)
                                                                                                                                     tctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCTTGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                  >  2:25104/1‑149 (MQ=255)
                                                                                                                                     tctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                  >  3:775687/1‑149 (MQ=255)
                                                                                                                                     tctTATTCGGCCTGGATTCCAGGCCCGGATTGCAACACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                  <  3:1587121/149‑1 (MQ=255)
                                                                                                                                      ctTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                 <  4:1272131/149‑1 (MQ=255)
                                                                                                                                          ttCGGCCTGGATTCCAGGCCCGGATTGAAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCAAGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat             >  2:1237649/1‑149 (MQ=255)
                                                                                                                                           cgggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGgcgc                                                        <  4:1669468/103‑1 (MQ=255)
                                                                                                                                            gggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca           >  2:1542738/2‑149 (MQ=255)
                                                                                                                                            gggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatca           >  2:244704/2‑149 (MQ=255)
                                                                                                                                             ggCCTGGAATCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat          >  4:135955/1‑149 (MQ=255)
                                                                                                                                               ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCg                         >  3:1189396/1‑132 (MQ=255)
                                                                                                                                                cTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc       <  3:645032/149‑1 (MQ=255)
                                                                                                                                                 tGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTcc      >  3:188571/1‑149 (MQ=255)
                                                                                                                                                 tGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTcc      >  1:1499007/1‑149 (MQ=255)
                                                                                                                                                  ggATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCa     >  2:994461/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAAAATTTCCCGAAATCCTTTTTCTATCGCCTGCGCAACCTCGCTGGCAGATAAACTTTCTTTATAAGAGTCTGGGGCGATTACGATTTTCATACCTATGCCTGTTACCACATGACGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAA  >  NC_000913/3271677‑3271973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: