Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,276,887 T→C 6.0% intergenic (+34/‑115) garD → / → prlF D‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,276,8870TC 6.0% 684.3 / 21.4 213intergenic (+34/‑115)garD/prlFD‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (102/97);  new base C (7/6);  total (110/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01

Only 100 of 217 total aligned reads displayed.

CTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGA  >  NC_000913/3276739‑3277035
                                                                                                                                                    |                                                                                                                                                    
cTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCt                                                                                                                                                      <  3:1570295/149‑1 (MQ=255)
 tGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAGACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCtt                                                                                                                                                     <  2:647434/149‑1 (MQ=255)
  ggAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTa                                                                                                                                                    <  4:1434039/149‑1 (MQ=255)
      gttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGc                                                                                                                                                >  1:114813/1‑149 (MQ=255)
       ttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGcc                                                                                                                                               >  1:265738/1‑149 (MQ=255)
            ccACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtt                                                                                                                                          <  3:1199503/149‑1 (MQ=255)
             cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGttt                                                                                                                                         >  1:934988/1‑149 (MQ=255)
             cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGttt                                                                                                                                         <  3:347864/149‑1 (MQ=255)
              aCTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGtttt                                                                                                                                        <  1:840464/149‑1 (MQ=255)
               cTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGttttt                                                                                                                                       <  2:1472560/149‑1 (MQ=255)
                tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTa                                                                                                                                      >  1:1252245/1‑149 (MQ=255)
                 ttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAt                                                                                                                                     <  1:1498066/149‑1 (MQ=255)
                       tcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                               >  4:513241/1‑149 (MQ=255)
                         gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTaaa                                                                                                                             <  2:1220936/149‑1 (MQ=255)
                         gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTaaa                                                                                                                             <  3:1004694/149‑1 (MQ=255)
                            gTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGcc                                                                                                                          <  2:1722977/149‑1 (MQ=255)
                              cGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGcccc                                                                                                                        >  3:1249047/1‑149 (MQ=255)
                                ccAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCa                                                                                                                      >  3:734242/1‑149 (MQ=255)
                                      ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCa                                                                                                                <  3:700287/149‑1 (MQ=255)
                                       gaagaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                               >  3:950077/1‑149 (MQ=255)
                                        aagaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAtt                                                                                                              <  3:507572/149‑1 (MQ=255)
                                          gaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaa                                                                                                            >  2:1483342/1‑149 (MQ=255)
                                            agaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaata                                                                                                          >  2:1319372/1‑149 (MQ=255)
                                             gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGccccc                                                                                                                       <  1:335208/135‑1 (MQ=255)
                                             gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                         >  2:985285/1‑149 (MQ=255)
                                             gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                         >  4:40031/1‑149 (MQ=255)
                                               aaaCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtg                                                                                                       >  2:483772/1‑149 (MQ=255)
                                                 aCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgca                                                                                                     <  2:1278688/149‑1 (MQ=255)
                                                    ttCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcattt                                                                                                  >  3:1602901/1‑149 (MQ=255)
                                                       tcGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgca                                                                                               >  2:620017/1‑149 (MQ=255)
                                                        cGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcat                                                                                              <  2:934988/149‑1 (MQ=255)
                                                        cGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGACACCGGTGACCTTATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcat                                                                                              <  4:1110607/149‑1 (MQ=255)
                                                           aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                           >  2:1605912/1‑149 (MQ=255)
                                                           aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                           <  2:341725/149‑1 (MQ=255)
                                                           aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTCCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                           >  1:1702760/1‑149 (MQ=255)
                                                            tCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGttt                                                                                                                                         <  1:1160500/102‑1 (MQ=255)
                                                            tCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGttt                                                                                                                                         >  2:1160500/1‑102 (MQ=255)
                                                             cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCt                                                                                         >  3:196058/1‑149 (MQ=255)
                                                              aaTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                        >  2:1012780/1‑149 (MQ=255)
                                                               aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                                       <  4:1070584/149‑1 (MQ=255)
                                                               aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                                       <  1:753807/149‑1 (MQ=255)
                                                                       tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGc                                                                               <  4:1010057/149‑1 (MQ=255)
                                                                          aTAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAATGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAAAAATGCATTTGCATTACCTTAGTTCAAGCTta                                                                            <  4:1034540/149‑1 (MQ=255)
                                                                           tAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGTTTTCTTTTGGAACGTCCCTCGCAACACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGCTCAAGCTtat                                                                           >  1:1192494/1‑149 (MQ=255)
                                                                                aGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAAttt                                                                      >  2:149120/1‑149 (MQ=255)
                                                                                aGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAAttt                                                                      <  1:566831/149‑1 (MQ=255)
                                                                                  cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                                    <  4:848637/149‑1 (MQ=255)
                                                                                  cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                                    <  2:265738/149‑1 (MQ=255)
                                                                                           tttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                           >  4:688690/1‑149 (MQ=255)
                                                                                           tttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                           >  1:919826/1‑149 (MQ=255)
                                                                                           tttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                           >  3:170452/1‑149 (MQ=255)
                                                                                            ttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACa                                                          <  1:828430/149‑1 (MQ=255)
                                                                                              aaCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                        >  2:685781/1‑117 (MQ=255)
                                                                                               aCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGc                                                       <  2:259515/149‑1 (MQ=255)
                                                                                                cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                      <  2:1252413/149‑1 (MQ=255)
                                                                                                cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                      <  1:1605912/149‑1 (MQ=255)
                                                                                                cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                      <  3:400726/149‑1 (MQ=255)
                                                                                                cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTAAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                      >  2:1352781/1‑149 (MQ=255)
                                                                                                  cGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGt                                                    <  3:1512363/149‑1 (MQ=255)
                                                                                                      aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                                >  4:239629/1‑149 (MQ=255)
                                                                                                      aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                                >  3:429567/1‑149 (MQ=255)
                                                                                                      aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                                <  4:186288/149‑1 (MQ=255)
                                                                                                        cGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAgg                                              <  3:1018149/149‑1 (MQ=255)
                                                                                                         ggTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGa                                             <  4:429567/149‑1 (MQ=255)
                                                                                                         ggTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGa                                             <  3:394531/149‑1 (MQ=255)
                                                                                                           tGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                           <  4:1297348/149‑1 (MQ=255)
                                                                                                            gACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAg                                          >  2:857075/1‑149 (MQ=255)
                                                                                                              ccTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTg                                        >  3:264625/1‑149 (MQ=255)
                                                                                                               cTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTta                                                                            <  1:1484908/112‑1 (MQ=255)
                                                                                                                tGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                                    <  2:1031992/119‑1 (MQ=255)
                                                                                                                tGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                                      >  1:605733/1‑149 (MQ=255)
                                                                                                                tGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                                      <  4:264625/149‑1 (MQ=255)
                                                                                                                tGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                                      >  2:796913/1‑149 (MQ=255)
                                                                                                                 gATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAAt                                     >  4:1624654/1‑149 (MQ=255)
                                                                                                                   tttCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                                       >  3:1416636/1‑97 (MQ=255)
                                                                                                                   tttCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCa                                   >  2:536422/1‑149 (MQ=255)
                                                                                                                     tCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATg                                 <  3:1523801/149‑1 (MQ=255)
                                                                                                                     tCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATg                                 <  1:1308124/149‑1 (MQ=255)
                                                                                                                      cTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGaaaa                                                            >  4:133802/1‑121 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGaa                                      <  4:495931/142‑1 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                               >  1:1624712/1‑149 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                               >  2:296723/1‑149 (MQ=255)
                                                                                                                       ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                               >  1:18130/1‑149 (MQ=255)
                                                                                                                            gAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGtt                                                                                    >  4:1506872/1‑91 (MQ=255)
                                                                                                                             aaCGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTaa                         >  3:868685/1‑149 (MQ=255)
                                                                                                                              aCGTCCCTCGCAAAACATGACCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAAt                        <  4:826615/149‑1 (MQ=255)
                                                                                                                                 tCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgct                     >  4:582798/1‑149 (MQ=255)
                                                                                                                                  cccTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctc                    <  4:1249047/149‑1 (MQ=255)
                                                                                                                                  cccTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctc                    <  1:440899/149‑1 (MQ=255)
                                                                                                                                   ccTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcg                   >  1:1568280/1‑149 (MQ=255)
                                                                                                                                    cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAg                                                                                                                            >  1:679891/1‑43 (MQ=255)
                                                                                                                                    cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgc                  <  1:1012780/149‑1 (MQ=255)
                                                                                                                                    cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgc                  >  2:888949/1‑149 (MQ=255)
                                                                                                                                      ataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACATCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgctc                >  3:1319116/4‑149 (MQ=255)
                                                                                                                                         aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                           >  2:1435684/1‑119 (MQ=255)
                                                                                                                                         aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa             >  3:1293941/1‑149 (MQ=255)
                                                                                                                                         aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa             >  3:60807/1‑149 (MQ=255)
                                                                                                                                                 gCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTAc     >  3:1106023/1‑149 (MQ=255)
                                                                                                                                                  ccTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACt    >  4:1535936/1‑149 (MQ=255)
                                                                                                                                                    ttAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGa  <  4:734242/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGA  >  NC_000913/3276739‑3277035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: