Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,563,539 T→C 6.1% intergenic (+21/+248) glpD → / ← yzgL sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,563,5390TC 6.1% 683.1 / 20.9 209intergenic (+21/+248)glpD/yzgLsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding/pseudogene, periplasmic solute binding protein homology
Reads supporting (aligned to +/- strand):  ref base T (88/107);  new base C (2/11);  total (91/118)
Fisher's exact test for biased strand distribution p-value = 4.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

CGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAA  >  NC_000913/3563393‑3563687
                                                                                                                                                  |                                                                                                                                                    
cgccgcgccgACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCa                                                                                                                                                    <  1:981955/149‑1 (MQ=255)
    gcgccgACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAtt                                                                                                                                                <  3:1658758/149‑1 (MQ=255)
    gcgccgACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAtt                                                                                                                                                <  1:932004/149‑1 (MQ=255)
     cgccgACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAttt                                                                                                                                               >  1:604807/1‑149 (MQ=255)
       ccgACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTACCGTAAGGTGATCAGGTCAGATTTCa                                                                                                                                             <  4:780959/149‑1 (MQ=255)
        cgacgaCGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCaa                                                                                                                                            >  1:1163131/1‑149 (MQ=255)
         gacgacGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAAt                                                                                                                                           >  3:287029/1‑149 (MQ=255)
            gacgCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTg                                                                                                                                        >  1:1242894/1‑149 (MQ=255)
              cgCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTgac                                                                                                                                      <  2:950596/149‑3 (MQ=255)
              cgCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTgac                                                                                                                                      <  1:1653598/149‑3 (MQ=255)
                  cTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTga                                                                                                                                  <  3:1533751/149‑1 (MQ=255)
                         gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                           <  3:645758/149‑1 (MQ=255)
                           cGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTAATCAGGTCAGATTTCAATCTGGCCTGAGACtgatga                                                                                                                         <  4:1010583/149‑1 (MQ=255)
                              acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaa                                                                                                                      >  4:1528791/1‑149 (MQ=255)
                              acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaa                                                                                                                      >  4:1360215/1‑149 (MQ=255)
                               caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACaaa                                                                                                                     <  1:857424/149‑1 (MQ=255)
                                aaaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGcc                                                                                                                                     <  4:1101146/132‑1 (MQ=255)
                                  aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAacac                                                                                                                  >  1:1485190/1‑149 (MQ=255)
                                  aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAacac                                                                                                                  >  2:1064822/1‑149 (MQ=255)
                                      aGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACaaaa                                                                                                              <  1:1427133/149‑1 (MQ=255)
                                       ggCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAAc                                                                                                             <  4:376408/149‑1 (MQ=255)
                                          aTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGc                                                                                                          <  3:1021016/149‑1 (MQ=255)
                                           tgtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGcc                                                                                                         <  3:631624/149‑1 (MQ=255)
                                              ggCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGa                                                                                                      >  1:1572530/1‑149 (MQ=255)
                                                cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATg                                                                                                    >  2:659355/1‑149 (MQ=255)
                                                     tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTa                                                                                               >  1:1623492/1‑149 (MQ=255)
                                                      gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTAc                                                                                              <  1:1064822/149‑1 (MQ=255)
                                                        ggATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGc                                                                                            >  4:1305849/1‑149 (MQ=255)
                                                        ggATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTCGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGc                                                                                            >  3:1337243/1‑149 (MQ=255)
                                                         gATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCt                                                                                           >  3:215239/1‑149 (MQ=255)
                                                          aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                           >  1:583286/1‑116 (MQ=255)
                                                          aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                           <  2:583286/116‑1 (MQ=255)
                                                          aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTAc                                                                                              <  4:1394654/145‑1 (MQ=255)
                                                           tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTa                                                                                         >  3:817098/1‑149 (MQ=255)
                                                            caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTAt                                                                                        <  2:1495078/149‑1 (MQ=255)
                                                            caacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTAt                                                                                        <  3:1389012/149‑1 (MQ=255)
                                                             aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                       >  2:1644774/1‑149 (MQ=255)
                                                             aacaaTCTCGTGTGAGTCAGTGGCTGGTCGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATc                                                                                       >  3:1078807/1‑149 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa                                                                                                                                                  >  4:1059216/1‑89 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtga                                                                                                                            >  1:346638/1‑111 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtga                                                                                                                            <  2:346638/111‑1 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATgc                                                                                                   <  2:563239/136‑1 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCa                                                                                      <  2:214811/149‑1 (MQ=255)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTgat                                                                                                                                 >  1:1412396/1‑105 (MQ=39)
                                                              acaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGACCTgat                                                                                                                                 <  2:1412396/106‑2 (MQ=39)
                                                                aaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAgg                                                                                    >  1:323088/1‑149 (MQ=255)
                                                                 aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGc                                                                                   <  4:940305/149‑1 (MQ=255)
                                                                 aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGAcc                                                                                                                                     <  3:1446241/99‑1 (MQ=255)
                                                                 aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGCCAGATTGAAATCTGAcc                                                                                                                                     >  4:1446241/1‑99 (MQ=255)
                                                                  tctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGcc                                                                                  <  2:1163131/149‑1 (MQ=255)
                                                                      gtgtGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACg                                                                              >  4:1539409/1‑149 (MQ=255)
                                                                         tgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgatga                                                                                                                         <  1:528952/103‑1 (MQ=255)
                                                                         tgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgatga                                                                                                                         >  2:528952/1‑103 (MQ=255)
                                                                         tgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTgg                                                                           <  1:1685815/149‑1 (MQ=255)
                                                                          gAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGt                                                                          <  3:937055/149‑1 (MQ=255)
                                                                             tCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTa                                                                       >  4:1197953/1‑149 (MQ=255)
                                                                              cAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTAt                                                                      <  2:1378164/149‑1 (MQ=255)
                                                                                gTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGc                                                                    >  2:682578/1‑149 (MQ=255)
                                                                                    cTGGTGGAGTATACGCAGCAGAGGTCATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCTTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAata                                                                <  3:1101633/149‑1 (MQ=255)
                                                                                      ggtggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAatata                                                              <  2:1279327/149‑1 (MQ=255)
                                                                                       gtggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAatatat                                                             >  3:825039/1‑149 (MQ=255)
                                                                                         ggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTgg                                                                           >  1:969934/1‑133 (MQ=255)
                                                                                         ggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGt                                                                          >  3:1598420/1‑134 (MQ=255)
                                                                                         ggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGt                                                                          <  4:1598420/134‑1 (MQ=255)
                                                                                         ggAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTg                                                           <  2:604807/149‑1 (MQ=255)
                                                                                          gAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGa                                                          <  4:1629961/149‑1 (MQ=255)
                                                                                          gAGTATACGCAGCAGAGGTTATCGCCGGCGTCGTAAATTAAAGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGa                                                          >  4:272190/1‑149 (MQ=255)
                                                                                            gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAt                                                        >  3:224014/1‑149 (MQ=255)
                                                                                            gTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAt                                                        >  1:1001651/1‑149 (MQ=255)
                                                                                             tataCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATgcgc                                                                                                 <  3:1270484/107‑1 (MQ=255)
                                                                                             tataCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATgcgc                                                                                                 >  4:1270484/1‑107 (MQ=255)
                                                                                              ataCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAttt                                                      <  2:1250785/149‑1 (MQ=255)
                                                                                                aCGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGc                                                    >  2:1654701/1‑149 (MQ=255)
                                                                                                 cGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCa                                                   <  4:224014/149‑1 (MQ=255)
                                                                                                  gcagcaGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                  >  1:715560/1‑149 (MQ=255)
                                                                                                     gcagAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGt                                               <  4:334007/149‑1 (MQ=255)
                                                                                                      cagAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTc                                              <  1:6705/149‑1 (MQ=255)
                                                                                                         agGTTATCGCTGGCGTCGTAAATTAATGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTAATGACAAACACAAAACTGCCTGATGCGCTACACTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCCTGGTCTTg                                           >  3:1047957/1‑149 (MQ=255)
                                                                                                          ggTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGGATTTGCATGGTCTTGt                                          <  3:1360215/149‑1 (MQ=255)
                                                                                                             tATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAgg                                       >  1:216520/1‑149 (MQ=255)
                                                                                                              aTCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGc                                      <  2:323088/149‑1 (MQ=255)
                                                                                                                     gagtcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATaa                               <  3:1305849/147‑1 (MQ=255)
                                                                                                                     gCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATaa                               <  1:1047078/149‑1 (MQ=255)
                                                                                                                       gtcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTTAATTTGCATGGTCTTGTAGGCCAGATAAGa                             <  3:558862/149‑1 (MQ=255)
                                                                                                                        tcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                  >  1:622727/1‑127 (MQ=255)
                                                                                                                        tcgtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                  <  2:622727/127‑1 (MQ=255)
                                                                                                                          gtAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACtgat                                                                                                                           <  4:829692/52‑1 (MQ=255)
                                                                                                                             aaTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAtt                                                       <  4:9677/117‑1 (MQ=255)
                                                                                                                             aaTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAAtt                                                       >  3:9677/1‑117 (MQ=255)
                                                                                                                             aaTTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCa                       >  3:836566/1‑149 (MQ=255)
                                                                                                                                    gTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCa                >  4:744773/1‑149 (MQ=255)
                                                                                                                                         gTGAGCAGGGCAGAGTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTAAGCTTATCAGGCATACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGAAGTTCACGTCGCAACCgg           >  2:1182835/1‑149 (MQ=21)
                                                                                                                                           gATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                  >  3:894278/1‑108 (MQ=255)
                                                                                                                                           gATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCAt                                                  <  4:894278/108‑1 (MQ=255)
                                                                                                                                           gATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCa         >  1:1026131/1‑149 (MQ=255)
                                                                                                                                            aTCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAg                                                                                     <  1:1317343/72‑1 (MQ=255)
                                                                                                                                                 gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTa                                         <  2:1531645/111‑1 (MQ=255)
                                                                                                                                                 gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTa                                         >  1:1531645/1‑111 (MQ=255)
                                                                                                                                                 gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACa   >  3:1528360/1‑149 (MQ=255)
                                                                                                                                                 gTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACa   <  1:1131297/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGCCGCGCCGACGACGCCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGATTTCAATCTGGCCTGAGACTGATGACAAACACAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGGTTTATGCAATATATTGAATTTGCATGGTCTTGTAGGCCAGATAAGACGTTCACGTCGCATCCGGCATGAACAA  >  NC_000913/3563393‑3563687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: